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gtf_tracking.h
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#ifndef GTF_TRACKING_H
#define GTF_TRACKING_H
/*
* gtf_tracking.h
*
*/
#ifdef HEAPROFILE
#include "gperftools/heap-profiler.h"
#endif
#include "gff.h"
#include "GFaSeqGet.h"
#include "GFastaIndex.h"
#include "GStr.h"
#define MAX_QFILES 500
extern bool gtf_tracking_verbose;
extern bool gtf_tracking_largeScale;
//many input files, no accuracy stats are generated, no *.tmap
// and exon attributes are discarded
int cmpByPtr(const pointer p1, const pointer p2);
bool t_contains(GffObj& a, GffObj& b);
//returns true only IF b has fewer exons than a AND a "contains" b
char* getGSeqName(int gseq_id);
//genomic fasta sequence handling
class GFastaHandler {
public:
char* fastaPath;
GFastaIndex* faIdx;
char* getFastaFile(int gseq_id) {
if (fastaPath==NULL) return NULL;
GStr s(fastaPath);
s.trimR('/');
s.appendfmt("/%s",getGSeqName(gseq_id));
GStr sbase(s);
if (!fileExists(s.chars())) s.append(".fa");
if (!fileExists(s.chars())) s.append("sta");
if (fileExists(s.chars())) return Gstrdup(s.chars());
else {
GMessage("Warning: cannot find genomic sequence file %s{.fa,.fasta}\n",sbase.chars());
return NULL;
}
}
GFastaHandler(const char* fpath=NULL) {
fastaPath=NULL;
faIdx=NULL;
if (fpath!=NULL && fpath[0]!=0) init(fpath);
}
void init(const char* fpath) {
if (fpath==NULL || fpath[0]==0) return;
if (!fileExists(fpath))
GError("Error: file/directory %s does not exist!\n",fpath);
fastaPath=Gstrdup(fpath);
if (fastaPath!=NULL) {
if (fileExists(fastaPath)>1) { //exists and it's not a directory
GStr fainame(fastaPath);
//the .fai name might have been given directly
if (fainame.rindex(".fai")==fainame.length()-4) {
//.fai index file given directly
fastaPath[fainame.length()-4]=0;
if (!fileExists(fastaPath))
GError("Error: cannot find fasta file for index %s !\n", fastaPath);
}
else fainame.append(".fai");
//fainame.append(".fai");
faIdx=new GFastaIndex(fastaPath,fainame.chars());
GStr fainamecwd(fainame);
int ip=-1;
if ((ip=fainamecwd.rindex('/'))>=0)
fainamecwd.cut(0,ip+1);
if (!faIdx->hasIndex()) { //could not load index
//try current directory
if (fainame!=fainamecwd) {
if (fileExists(fainamecwd.chars())>1) {
faIdx->loadIndex(fainamecwd.chars());
}
}
} //tried to load index
if (!faIdx->hasIndex()) {
GMessage("No fasta index found for %s. Rebuilding, please wait..\n",fastaPath);
faIdx->buildIndex();
if (faIdx->getCount()==0) GError("Error: no fasta records found!\n");
GMessage("Fasta index rebuilt.\n");
FILE* fcreate=fopen(fainame.chars(), "w");
if (fcreate==NULL) {
GMessage("Warning: cannot create fasta index %s! (permissions?)\n", fainame.chars());
if (fainame!=fainamecwd) fcreate=fopen(fainamecwd.chars(), "w");
if (fcreate==NULL)
GError("Error: cannot create fasta index %s!\n", fainamecwd.chars());
}
if (faIdx->storeIndex(fcreate)<faIdx->getCount())
GMessage("Warning: error writing the index file!\n");
} //index created and attempted to store it
} //multi-fasta
} //genomic sequence given
}
GFaSeqGet* fetch(int gseq_id, bool checkFasta=false) {
if (fastaPath==NULL) return NULL;
//genomic sequence given
GFaSeqGet* faseq=NULL;
if (faIdx!=NULL) { //fastaPath was the multi-fasta file name
char* gseqname=getGSeqName(gseq_id);
GFastaRec* farec=faIdx->getRecord(gseqname);
if (farec!=NULL) {
faseq=new GFaSeqGet(fastaPath,farec->seqlen, farec->fpos,
farec->line_len, farec->line_blen);
faseq->loadall(); //just cache the whole sequence, it's faster
}
else {
GMessage("Warning: couldn't find fasta record for '%s'!\n",gseqname);
return NULL;
}
}
else //if (fileExists(fastaPath)==1)
{
char* sfile=getFastaFile(gseq_id);
if (sfile!=NULL) {
//if (gtf_tracking_verbose)
// GMessage("Processing sequence from fasta file '%s'\n",sfile);
faseq=new GFaSeqGet(sfile,checkFasta);
faseq->loadall();
GFREE(sfile);
}
} //one fasta file per contig
return faseq;
}
~GFastaHandler() {
GFREE(fastaPath);
delete faIdx;
}
};
bool betterRef(GffObj* a, GffObj* b); //for better CovLink reference ranking
class GLocus;
class COvLink {
public:
static int coderank(char c) {
switch (c) {
case '=': return 0; //ichain match
case 'c': return 2; //containment (ichain fragment)
case 'j': return 4; // overlap with at least a junction match
case 'e': return 6; // single exon transfrag overlapping an intron of reference (possible pre-mRNA)
case 'o': return 8; // generic exon overlap
case 's': return 16; //"shadow" - an intron overlaps with a ref intron on the opposite strand
case 'x': return 18; // exon overlap on opposite strand (usually wrong strand mapping)
case 'i': return 20; // intra-intron
case 'p': return 90; //polymerase run
case 'r': return 92; //repeats
case 'u': return 94; //intergenic
case 0 : return 100;
default: return 96;
}
}
char code;
int rank;
GffObj* mrna;
int ovlen;
COvLink(char c=0,GffObj* m=NULL, int ovl=0) {
code=c;
mrna=m;
ovlen=ovl;
rank=coderank(c);
}
bool operator<(COvLink& b) {
if (rank==b.rank)
return (ovlen==b.ovlen)? betterRef(mrna, b.mrna) : (ovlen>b.ovlen);
else return rank<b.rank;
}
bool operator==(COvLink& b) {
return (rank==b.rank && mrna==b.mrna);
}
};
class GISeg: public GSeg {
public:
GffObj* t; //pointer to the largest transcript with a segment this exact exon coordinates
GISeg(uint s=0,uint e=0, GffObj* ot=NULL):GSeg(s,e) { t=ot; }
};
class GIArray:public GArray<GISeg> {
public:
GIArray(bool uniq=true):GArray<GISeg>(true,uniq) { }
int IAdd(GISeg* item) {
if (item==NULL) return -1;
int result=-1;
if (Found(*item, result)) {
if (fUnique) {
//cannot add a duplicate, return index of existing item
if (item->t!=NULL && fArray[result].t!=NULL &&
item->t->covlen>fArray[result].t->covlen)
fArray[result].t=item->t;
return result;
}
}
//Found sets result to the position where the item should be
idxInsert(result, *item);
return result;
}
};
class CEqList: public GList<GffObj> {
public:
GffObj* head;
CEqList():GList<GffObj>((GCompareProc*)cmpByPtr, (GFreeProc*)NULL, true) {
head=NULL;
}
};
class CTData { //transcript associated data
public:
GffObj* mrna; //owner transcript
GLocus* locus;
GList<COvLink> ovls; //overlaps with other transcripts (ref vs query)
//-- just for ichain match tracking:
GffObj* eqref; //ref transcript having an ichain match
int qset; //qry set index (qfidx), -1 means reference dataset
//GffObj* eqnext; //next GffObj in the linked list of matching transfrags
bool eqhead;
CEqList* eqlist; //keep track of matching transfrags
//int eqdata; // flags for EQ list (is it a list head?)
// Cufflinks specific data:
double FPKM;
double conf_hi;
double conf_lo;
double cov;
char classcode; //the best/final classcode
CTData(GffObj* m=NULL, GLocus* l=NULL):ovls(true,true,true) {
mrna=m;
if (mrna!=NULL) mrna->uptr=this;
locus=l;
classcode=0;
eqref=NULL;
eqhead=false;
eqlist=NULL;
qset=-2;
FPKM=0;
conf_lo=0;
conf_hi=0;
cov=0;
}
~CTData() {
ovls.Clear();
//if ((eqdata & EQHEAD_TAG)!=0) delete eqlist;
//if (isEqHead()) delete eqlist;
if (eqhead) delete eqlist;
}
//inline bool eqHead() { return ((eqdata & EQHEAD_TAG)!=0); }
/* bool isEqHead() {
if (eqlist==NULL) return false;
return (eqlist->head==this->mrna);
}
*/
void joinEqList(GffObj* m) { //add list from m
//list head is set to the transfrag with the lower qset#
CTData* md=(CTData*)(m->uptr);
//ASSERT(md);
if (eqlist==NULL) { //no eqlist yet for this node
if (md->eqlist!=NULL) { //m in an eqlist already
eqlist=md->eqlist;
eqlist->Add(this->mrna);
CTData* md_head_d=(CTData*)(md->eqlist->head->uptr);
if (this->qset < md_head_d->qset) {
eqlist->head=this->mrna;
eqhead=true;
md_head_d->eqhead=false;
}
}
else { //m was not in an EQ list either
eqlist=new CEqList();
eqlist->Add(this->mrna);
eqlist->Add(m);
md->eqlist=eqlist;
if (qset<md->qset) {
eqlist->head=this->mrna;
eqhead=true;
}
else {
eqlist->head=m;
md->eqhead=true;
}
}
}//no eqlist before
else { //merge two eqlists
if (eqlist==md->eqlist) //already in the same eqlist, nothing to do
return;
if (md->eqlist!=NULL) {
//copy the smaller list into the larger one
CEqList* srclst, *destlst;
if (md->eqlist->Count()<eqlist->Count()) {
srclst=md->eqlist;
destlst=eqlist;
}
else {
srclst=eqlist;
destlst=md->eqlist;
}
for (int i=0;i<srclst->Count();i++) {
destlst->Add(srclst->Get(i));
CTData* od=(CTData*)((*srclst)[i]->uptr);
od->eqlist=destlst;
}
this->eqlist=destlst;
CTData* s_head_d=(CTData*)(srclst->head->uptr);
CTData* d_head_d=(CTData*)(destlst->head->uptr);
if (s_head_d->qset < d_head_d->qset ) {
this->eqlist->head=srclst->head;
s_head_d->eqhead=true;
d_head_d->eqhead=false;
}
else {
s_head_d->eqhead=false;
d_head_d->eqhead=true;
}
delete srclst;
}
else { //md->eqlist==NULL
eqlist->Add(m);
md->eqlist=eqlist;
CTData* head_d=(CTData*)(eqlist->head->uptr);
if (md->qset<head_d->qset) {
eqlist->head=m;
md->eqhead=true;
}
}
}
}
void addOvl(char code,GffObj* target=NULL, int ovlen=0) {
ovls.AddIfNew(new COvLink(code, target, ovlen));
}
char getBestCode() {
return (ovls.Count()>0) ? ovls[0]->code : 0 ;
}
bool operator<(CTData& b) { return (mrna < b.mrna); }
bool operator==(CTData& b) { return (mrna==b.mrna); }
};
class GSuperLocus;
class GTrackLocus;
class GXLocus;
class GXSeg : public GSeg {
public:
int flags;
GXSeg(uint s=0, uint e=0, int f=0):GSeg(s,e),flags(f) { }
};
//Data structure holding a query locus data (overlapping mRNAs on the same strand)
// and also the accuracy data of all mRNAs of a query locus
// (against all reference loci overlapping the same region)
class GLocus:public GSeg {
public:
int gseq_id; //id of underlying genomic sequence
int qfidx; // for locus tracking
GTrackLocus* t_ptr; //for locus tracking cluster
GffObj* mrna_maxcov; //transcript with maximum coverage (for main "ref" transcript)
GffObj* mrna_maxscore; //transcript with maximum gscore (for major isoform)
GList<GffObj> mrnas; //list of transcripts (isoforms) for this locus
GArray<GXSeg> uexons; //list of unique exons (covered segments) in this region
GArray<GSeg> mexons; //list of merged exons in this region
GIArray introns;
GList<GLocus> cmpovl; //temp list of overlapping qry/ref loci to compare to (while forming superloci)
//only for reference loci --> keep track of all superloci found for each qry dataset
// which contain this reference locus
GList<GSuperLocus>* superlst;
GXLocus* xlocus; //superlocus formed by exon overlaps across all qry datasets
// -- if genomic sequence was given:
int spl_major; // number of GT-AG splice site consensi
int spl_rare; // number of GC-AG, AT-AC and other rare splice site consensi
int spl_wrong; //number of "wrong" (unrecognized) splice site consensi
int ichains; //number of multi-exon mrnas
int ichainTP; //number of intron chains fully matching reference introns
//int ichainATP;
int mrnaTP;
//int mrnaATP;
int v; //user flag/data
GLocus(GffObj* mrna=NULL, int qidx=-1):mrnas(true,false,false),uexons(true,true),mexons(true,true),
introns(), cmpovl(true,false,true) {
//this will NOT free mrnas!
ichains=0;
gseq_id=-1;
qfidx=qidx;
t_ptr=NULL;
creset();
xlocus=NULL;
mrna_maxcov=NULL;
mrna_maxscore=NULL;
superlst=new GList<GSuperLocus>(true,false,false);
if (mrna!=NULL) {
start=mrna->exons.First()->start;
end=mrna->exons.Last()->end;;
gseq_id=mrna->gseq_id;
GISeg seg;
for (int i=0;i<mrna->exons.Count();i++) {
seg.start=mrna->exons[i]->start;
seg.end=mrna->exons[i]->end;
int flags=0; //terminal exon flags (1=left end, 2=right end)
if (i==0) flags|=1;
if (i==mrna->exons.Count()-1) flags|=2;
GXSeg xseg(seg.start, seg.end, flags);
uexons.Add(xseg);
mexons.Add(seg);
if (i>0) {
seg.start=mrna->exons[i-1]->end+1;
seg.end=mrna->exons[i]->start-1;
seg.t=mrna;
introns.Add(seg);
}
}
mrnas.Add(mrna);
if (mrna->exons.Count()>1) ichains++;
((CTData*)(mrna->uptr))->locus=this;
mrna_maxscore=mrna;
mrna_maxcov=mrna;
}
}
~GLocus() {
delete superlst;
}
void creset() {
spl_major=0;spl_rare=0;spl_wrong=0;
v=0; //visited/other data
ichainTP=0;
//ichainATP=0;
mrnaTP=0;
//mrnaATP=0;
cmpovl.Clear();
}
void addMerge(GLocus& locus, GffObj* lnkmrna) {
//add all the elements of the other locus (merging)
//-- merge mexons
GArray<int> ovlexons(true,true); //list of locus.mexons indexes overlapping existing mexons
int i=0; //index of first mexons with a merge
int j=0; //index current mrna exon
while (i<mexons.Count() && j<locus.mexons.Count()) {
uint istart=mexons[i].start;
uint iend=mexons[i].end;
uint jstart=locus.mexons[j].start;
uint jend=locus.mexons[j].end;
if (iend<jstart) { i++; continue; }
if (jend<istart) { j++; continue; }
//if (mexons[i].overlap(jstart, jend)) {
//exon overlap was found :
ovlexons.Add(j);
//extend mexons[i] as needed
if (jstart<istart) mexons[i].start=jstart;
if (jend>iend) { //mexons[i] end extend
mexons[i].end=jend;
//now this could overlap the next mexon(s), so we have to merge them all
while (i<mexons.Count()-1 && mexons[i].end>mexons[i+1].start) {
uint nextend=mexons[i+1].end;
mexons.Delete(i+1);
if (nextend>mexons[i].end) {
mexons[i].end=nextend;
break; //no need to check next mexons
}
} //while next mexons merge
} // mexons[i] end extend
// } //exon overlap
j++; //check the next locus.mexon
}
//-- add the rest of the non-overlapping mexons:
GSeg seg;
for (int i=0;i<locus.mexons.Count();i++) {
seg.start=locus.mexons[i].start;
seg.end=locus.mexons[i].end;
if (!ovlexons.Exists(i)) mexons.Add(seg);
}
// -- merge uexons
//add to uexons:
for (int i=0;i<locus.uexons.Count();i++) {
uexons.Add(locus.uexons[i]);
}
for (int i=0;i<locus.introns.Count();i++) {
introns.IAdd(&(locus.introns[i]));
}
// -- add locus.mrnas
for (int i=0;i<locus.mrnas.Count();i++) {
((CTData*)(locus.mrnas[i]->uptr))->locus=this;
if (locus.mrnas[i]!=lnkmrna) {
mrnas.Add(locus.mrnas[i]);
if (locus.mrnas[i]->exons.Count()>1) ichains++;
}
}
// -- adjust start/end as needed
if (start>locus.start) start=locus.start;
if (end<locus.end) end=locus.end;
if (mrna_maxcov->covlen<locus.mrna_maxcov->covlen)
mrna_maxcov=locus.mrna_maxcov;
if (mrna_maxscore->gscore<locus.mrna_maxscore->gscore)
mrna_maxscore=locus.mrna_maxscore;
}
bool exonOverlap(GLocus& loc) {
//check if any mexons overlap!
int i=0;
int j=0;
while (i<mexons.Count() && j<loc.mexons.Count()) {
uint istart=mexons[i].start;
uint iend=mexons[i].end;
uint jstart=loc.mexons[j].start;
uint jend=loc.mexons[j].end;
if (iend<jstart) { i++; continue; }
if (jend<istart) { j++; continue; }
//exon overlap found if we're here:
return true;
}
return false;
}
bool add_mRNA(GffObj* mrna) {
if (mrnas.Count()>0 && mrna->gseq_id!=gseq_id) return false; //mrna must be on the same genomic seq
//check for exon overlap with existing mexons
//also update uexons and mexons accordingly, if mrna is added
uint mrna_start=mrna->exons.First()->start;
uint mrna_end=mrna->exons.Last()->end;
if (mrna_start>end || start>mrna_end) return false;
bool hasovl=false;
int i=0; //index of first mexons with a merge
int j=0; //index current mrna exon
GArray<int> ovlexons(true,true); //list of mrna exon indexes overlapping mexons
while (i<mexons.Count() && j<mrna->exons.Count()) {
uint istart=mexons[i].start;
uint iend=mexons[i].end;
uint jstart=mrna->exons[j]->start;
uint jend=mrna->exons[j]->end;
if (iend<jstart) { i++; continue; }
if (jend<istart) { j++; continue; }
//exon overlap found if we're here:
ovlexons.Add(j);
hasovl=true;
//extend mexons[i] as needed
if (jstart<istart) mexons[i].start=jstart;
if (jend>iend) { //mexon stretch up
mexons[i].end=jend;
//now this could overlap the next mexon(s), so we have to merge them all
while (i<mexons.Count()-1 && mexons[i].end>mexons[i+1].start) {
uint nextend=mexons[i+1].end;
mexons.Delete(i+1);
if (nextend>mexons[i].end) {
mexons[i].end=nextend;
break; //no need to check next mexons
}
} //while next mexons merge
} //possible mexons merge
j++; //check the next mrna exon
}//all vs all exon check loop
if (hasovl) {
GSeg seg;
//add the rest of the non-overlapping exons,
// and also to uexons etc.
for (int i=0;i<mrna->exons.Count();i++) {
seg.start=mrna->exons[i]->start;
seg.end=mrna->exons[i]->end;
if (!ovlexons.Exists(i)) mexons.Add(seg);
int xterm=0;
if (i==0) xterm|=1;
if (i==mrna->exons.Count()-1) xterm|=2;
GXSeg xseg(seg.start, seg.end, xterm);
uexons.Add(xseg);
GISeg iseg;
if (i>0) {
iseg.start=mrna->exons[i-1]->end+1;
iseg.end=mrna->exons[i]->start-1;
iseg.t=mrna;
introns.Add(iseg);
}
}
mrnas_add(mrna);
// add to mrnas
((CTData*)mrna->uptr)->locus=this;
gseq_id=mrna->gseq_id;
if (mrna->exons.Count()>1) ichains++;
}
return hasovl;
}
//simpler,basic adding of a mrna
void mrnas_add(GffObj* mrna) {
mrnas.Add(mrna);
// adjust start/end
if (start>mrna->start) start=mrna->start;
if (end<mrna->end) end=mrna->end;
if (mrna_maxcov->covlen<mrna->covlen) mrna_maxcov=mrna;
if (mrna_maxscore->gscore<mrna->gscore) mrna_maxscore=mrna;
}
};
class GSuperLocus;
class GTrackLocus;
class GSuperLocus : public GSeg {
public:
int qfidx; //index of query dataset/file for which this superlocus was created
GList<GLocus> qloci;
GList<GLocus> rloci;
GList<GffObj> qmrnas; //list of transcripts (isoforms) for this locus
GArray<GSeg> qmexons; //list of merged exons in this region
GArray<GXSeg> quexons; //list of unique exons (covered segments) in this region
GIArray qintrons; //list of unique introns in this region
//same lists for reference:
GList<GffObj> rmrnas; //list of ref transcripts (isoforms) for this locus
GArray<GSeg> rmexons; //list of ref merged exons in this region
GArray<GXSeg> ruexons; //list of ref unique exons (covered segments) in this region
GArray<GISeg> rintrons; //list of unique introns in this region
// store problematic introns for printing:
GIArray i_missed; //missed reference introns (not overlapped by any qry intron)
GIArray i_notp; //wrong ref introns (one or both ends not matching any qry intron)
//
GIArray i_qwrong; //totally wrong qry introns (not overlapped by any ref intron)
GIArray i_qnotp; //imperfect qry introns (may overlap but has no "perfect" match)
int qbases_all;
int rbases_all; //in fact, it's all ref bases overlapping any query loci
int in_rmrnas; //count of ALL ref mrnas and loci given for this region
int in_rloci; //not just those overlapping qry data
// this will keep track of total qry loci, mrnas and exons in an area
int total_superloci;
int total_qloci;
int total_qloci_alt; //total qloci with multiple transcripts
int total_qmrnas;
int total_qexons; //unique exons
int total_qmexons;
int total_qintrons; //unique introns
int total_qichains; //total multi-exon transfrags predicted (incl. duplicates if -G)
// NOTE: these ref totals are only limited to data from loci overlapping any qry loci
int total_rmexons;
int total_rloci;
int total_rmrnas;
int total_richains; //total multi-exon reference transcripts
int total_rexons;
int total_rintrons; //unique introns
//--- accuracy data after compared to ref loci:
int locusQTP;
int locusTP; // +1 if ichainTP+mrnaTP > 0
//int locusAQTP;
//int locusATP; // 1 if ichainATP + mrnaATP > 0
int locusFP;
//int locusAFP;
//int locusAFN;
int locusFN;
//---transcript level accuracy -- all exon coordinates should match (most stringent)
int mrnaTP; // number of qry mRNAs with perfect match with ref transcripts
//int mrnaATP;
//---intron level accuracy (comparing the ordered set of splice sites):
int ichainTP; // number of fully matched ref intron chains (# correctly predicted ichains)
//int ichainFP; // number of qry intron chains not matching a reference intron chain
//int ichainFN; // number of ref intron chains in this region not being covered by a reference intron chain
/*
// same as above, but Approximate -- allowing a 5bp distance around splice site coordinates
int ichainATP; //as opposed to ichainTP, this also includes ref intron chains which are
//sub-chains of qry intron chains (rare cases)
*/
//---projected features ---
//---exon level accuracy:
int exonTP; //number of matched reference exons (true positives)
int exonQTP; //number of query exons matching reference exons
//int exonFP; //number of exons of query with no perfect match with a reference exon
//int exonFN; //number of exons of reference with no perfect match with a query exon
// same as the above but with acceptable approximation (10bp error window):
/*int exonATP;
int exonAFP;
int exonAFN;*/
int intronTP; //number of perfectly overlapping introns (true positives)
int intronFP; //number of introns of query with no perfect match with a reference intron
int intronFN; //number of introns of reference with no perfect match with a query intron
/*
// same as the above but with acceptable approximation (10bp error window):
int intronATP;
int intronAFP;
int intronAFN;
*/
//-- EGASP added these too:
int m_exons; //number of exons totally missed (not overlapped *at all* by any query exon)
int w_exons; //numer of totally wrong exons (query exons not overlapping *at all* any reference exon)
int m_introns; //number of introns totally missed (not overlapped *at all* by any query intron)
int w_introns; //numer of totally wrong introns (query introns not overlapping *at all* any reference intron)
int m_loci; //missed loci
int w_loci; //novel/wrong loci
//---base level accuracy
int baseTP; //number of overlapping bases
int baseFP; //number of qry bases not overlapping reference
int baseFN; //number of ref bases not overlapping qry
// sorted,free,unique sorted,unique
GSuperLocus(uint lstart=0,uint lend=0):qloci(true,false,false),rloci(true,false,false),
qmrnas(true,false,false), qmexons(true,false), quexons(true,false), qintrons(false),
rmrnas(true,false,false), rmexons(true,false), ruexons(true,false), rintrons(false),
i_missed(false),i_notp(false), i_qwrong(false), i_qnotp(false){
qfidx=-1;
start=lstart;
end=lend;
qbases_all=0;
rbases_all=0;
baseTP=0;baseFP=0;baseFN=0;
locusTP=0;locusQTP=0; //locusAQTP=0; locusATP=0;
locusFP=0;// locusAFP=0;locusAFN=0;
locusFN=0;
in_rmrnas=0;
in_rloci=0;
w_loci=0;
m_loci=0;
total_superloci=0;
mrnaTP=0;//mrnaFP=0;mrnaFN=0;
ichainTP=0;//ichainFP=0;ichainFN=0;
exonTP=0;exonQTP=0;
//exonFP=0;exonFN=0;
intronTP=0;intronFP=0;intronFN=0;
/* mrnaATP=0;//mrnaAFP=0;mrnaAFN=0;
ichainATP=0;//ichainAFP=0;ichainAFN=0;
exonATP=0;exonAFP=0;exonAFN=0;
intronATP=0;intronAFP=0;intronAFN=0; */
total_rmexons=0;
total_qmexons=0;
total_qexons=0;total_qloci=0;total_qmrnas=0;
total_qloci_alt=0;
total_qintrons=0;total_qichains=0;
total_rexons=0;total_rloci=0;total_rmrnas=0;
total_rintrons=0;total_richains=0;
w_exons=0;
m_exons=0;
w_introns=0;
m_introns=0;
}
void addQlocus(GLocus& loc) {
if (start==0 || start>loc.start) start=loc.start;
if (end<loc.end) end=loc.end;
qloci.Add(&loc);
total_qloci++;
if (loc.ichains>0 && loc.mrnas.Count()>1)
total_qloci_alt++;
qmrnas.Add(loc.mrnas);
total_qmrnas+=loc.mrnas.Count();
total_qichains+=loc.ichains;
qmexons.Add(loc.mexons);
total_qmexons+=loc.mexons.Count();
quexons.Add(loc.uexons);
total_qexons+=loc.uexons.Count();
qintrons.Add(loc.introns);
total_qintrons+=loc.introns.Count();
}
void addRlocus(GLocus& loc) {
if (start==0 || start>loc.start) start=loc.start;
if (end<loc.end) end=loc.end;
rloci.Add(&loc);
total_rloci++;
rmrnas.Add(loc.mrnas);
total_rmrnas+=loc.mrnas.Count();
total_richains+=loc.ichains;
rmexons.Add(loc.mexons);
total_rmexons+=loc.mexons.Count();
ruexons.Add(loc.uexons);
total_rexons+=loc.uexons.Count();
rintrons.Add(loc.introns);
total_rintrons+=loc.introns.Count();
}
void calcF() {
// base level
baseFP=qbases_all-baseTP;
baseFN=rbases_all-baseTP;
//exon level:
//exonFP=total_qexons-exonTP;
//exonFN=total_rexons-exonTP;
//intron stats
intronFP=total_qintrons-intronTP;
intronFN=total_rintrons-intronTP;
/* intronAFN=total_rintrons-intronATP;
intronAFP=total_qintrons-intronATP;
exonAFP=total_qexons-exonATP;
exonAFN=total_rexons-exonATP; */
// ichain and transcript levels:
//ichainAFP=total_qichains-ichainATP;
//ichainFP=total_qichains-ichainTP;
//ichainAFN=total_richains-ichainATP;
//ichainFN=total_richains-ichainTP;
//mrnaFP=total_qmrnas-mrnaTP;
//mrnaFN=total_rmrnas-mrnaTP;
//mrnaAFP=total_qmrnas-mrnaATP;
//mrnaAFN=total_rmrnas-mrnaATP;
// locus/gene level:
locusFP=total_qloci-locusQTP;
/*locusAFN=total_rloci-locusATP;
locusAFP=total_qloci-locusAQTP;*/
locusFN=total_rloci-locusTP;
}
void addStats(GSuperLocus& s) {
in_rmrnas+=s.in_rmrnas;
in_rloci+=s.in_rloci;
baseTP+=s.baseTP;
exonTP+=s.exonTP;
exonQTP+=s.exonQTP;
intronTP+=s.intronTP;
ichainTP+=s.ichainTP;
mrnaTP+=s.mrnaTP;
locusTP+=s.locusTP;
locusQTP+=s.locusQTP;
/*
mrnaATP+=s.mrnaATP;
exonATP+=s.exonATP;
intronATP+=s.intronATP;
ichainATP+=s.ichainATP;
locusATP+=s.locusATP;
locusAQTP+=s.locusAQTP;
*/
m_exons+=s.m_exons;
w_exons+=s.w_exons;
m_introns+=s.m_introns;
w_introns+=s.w_introns;
if (s.total_superloci==0 && s.qloci.Count()>0) s.total_superloci=1;
total_superloci+=s.total_superloci;
qbases_all+=s.qbases_all;
rbases_all+=s.rbases_all;
m_loci+=s.m_loci;
w_loci+=s.w_loci;
total_qexons+=s.total_qexons;
total_qintrons+=s.total_qintrons;
total_qmexons+=s.total_qmexons;
total_rexons+=s.total_rexons;
total_rintrons+=s.total_rintrons;
total_rmexons+=s.total_rmexons;
total_qmrnas+=s.total_qmrnas;
total_qichains+=s.total_qichains;
total_rmrnas+=s.total_rmrnas;
total_richains+=s.total_richains;
total_qloci+=s.total_qloci;
total_qloci_alt+=s.total_qloci_alt;
total_rloci+=s.total_rloci;
}
};
class GSeqData {
int gseq_id;
public:
const char* gseq_name;
GList<GffObj> refs_f; //forward strand mRNAs
GList<GffObj> refs_r; //reverse strand mRNAs
GList<GffObj> mrnas_f; //forward strand mRNAs
GList<GffObj> mrnas_r; //reverse strand mRNAs
GList<GLocus> loci_f; //forward strand loci
GList<GLocus> loci_r; //reverse strand loci
//--> the fields below are not used by reference data --
GList<GSuperLocus> gstats_f; //stats for forward strand superloci
GList<GSuperLocus> gstats_r; //stats for reverse strand superloci
GList<GLocus> nloci_f; //"novel" loci on forward strand
GList<GLocus> nloci_r; //"novel" loci on reverse strand
GList<GffObj> umrnas; //unknown orientation mrnas
GList<GLocus> nloci_u; //"novel" loci with no orientation found
GList<CTData> tdata; //transcript data (uptr holder for all mrnas here)
int get_gseqid() { return gseq_id; }
//--<
GSeqData(int gid=-1):mrnas_f(true,true,false),mrnas_r(true,true,false),
loci_f(true,true,true),loci_r(true,true,true),
gstats_f(true,true,false),gstats_r(true,true,false),
nloci_f(true,false,true), nloci_r(true,false,true),
umrnas(true,true,false), nloci_u(true,true,true), tdata(false,true,false) {
gseq_id=gid;
if (gseq_id>=0)
gseq_name=GffObj::names->gseqs.getName(gseq_id);
}
bool operator==(GSeqData& d){
return (gseq_id==d.gseq_id);
}
bool operator>(GSeqData& d){
return (gseq_id>d.gseq_id);
}
bool operator<(GSeqData& d){
return (gseq_id<d.gseq_id);
}
};
// a group of qry loci and a transcript cluster for a single qry dataset
class GQCluster : public GList<GffObj> {
public:
GffObj* mrna_maxcov; //transcript with maximum coverage (for largest transcript)
GffObj* mrna_maxscore; //transcript with maximum gscore ( = major isoform for Cufflinks)
uint start;
uint end;
GList<GLocus> qloci;
//GCluster cl; //just a more compact way of keeping all transcripts in these loci
GQCluster(GList<GLocus>* loci=NULL):GList<GffObj>(true,false,false),
qloci(true,false,false) {
mrna_maxcov=NULL;
mrna_maxscore=NULL;
start=0;
end=0;
if (loci!=NULL) {
qloci.Add(*loci);
for (int i=0;i<loci->Count();i++) {
addLocus(loci->Get(i),false);
}
}
}
void addLocus(GLocus* loc, bool toLoci=true) {
//check so we don't add locus duplicates
if (toLoci) {
for (int i=0;i<qloci.Count();i++) {
if (loc==qloci[i]) return;
}
qloci.Add(loc);
}
for (int m=0;m<loc->mrnas.Count();m++) {
GffObj* mrna=loc->mrnas[m];
Add(mrna);
if (start==0 || start>mrna->start) start=mrna->start;
if (end<mrna->end) end=mrna->end;
if (mrna_maxcov==NULL || mrna_maxcov->covlen<mrna->covlen) mrna_maxcov=mrna;
if (mrna_maxscore==NULL || mrna_maxscore->gscore<mrna->gscore) mrna_maxscore=mrna;
}
}
};
//track a set of clustered qloci across multiple qry datasets
// the qloci in qcls[] overlap but not necessarily at exon level
// (so there can be multiple genes here in fact)
class GTrackLocus:public GSeg {
public:
char strand;
bool hasQloci;
//GLocus* rloc; //corresponding reference locus, if available
GList<GLocus> rloci; //ref loci found overlapping this region
GQCluster* qcls[MAX_QFILES]; //all qloci for this superlocus, grouped by dataset
GTrackLocus(GLocus* qloc=NULL, int q=-1):GSeg(0,0),rloci(true,false,true) {
strand='.';
for (int i=0;i<MAX_QFILES;i++) qcls[i]=NULL;
if (qloc!=NULL) addQLocus(qloc,q);
}
void addRLocus(GLocus* rl) {
if (rl==NULL) return;
if (rl->qfidx>=0)
GError("Error: GTrackLocus::addRLocus called with a query locus (set# %d)\n",
rl->qfidx+1);
if (strand=='.') strand=rl->mrna_maxcov->strand;
if (start==0 || start>rl->start) start=rl->start;
if (end==0 || end<rl->end) end=rl->end;
rl->t_ptr=this;
rloci.Add(rl);
}
void addQLocus(GLocus* loc, int q=-1) { //adding qry locus
if (loc==NULL) return;
if (strand=='.' && loc->mrna_maxcov->strand!='.')
strand=loc->mrna_maxcov->strand;
if (loc->qfidx<0 && q<0)
GError("Error at GTrackLocus::addQLocus(): locus.qfidx not set and index not given!\n");
if (q>=0) loc->qfidx=q;
else q=loc->qfidx;
if (start==0 || start>loc->start) start=loc->start;
if (end==0 || end<loc->end) end=loc->end;
if (qcls[q]==NULL) qcls[q]=new GQCluster();
hasQloci=true;
loc->t_ptr = this;
qcls[q]->addLocus(loc);
}
bool add_Locus(GLocus* loc) {
if (start==0 || overlap(*loc)) { //simple range overlap, not exon overlap
if (loc->qfidx<0) addRLocus(loc);
else addQLocus(loc);
return true;
}
return false;
}