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addgenesubcloning 6.owl
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<rdf:RDF
xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
xmlns="http://www.semanticweb.org/ad/ontologies/2018/3/untitled-ontology-6#"
xmlns:owl="http://www.w3.org/2002/07/owl#"
xmlns:www="http://www.wet.protocol#"
xmlns:rdfs="http://www.w3.org/2000/01/rdf-schema#"
xmlns:xsd="http://www.w3.org/2001/XMLSchema#">
<owl:Ontology rdf:about="http://www.wet.protocol"/>
<owl:Class rdf:about="http://www.wet.protocol#PipetteRelease">
<rdfs:subClassOf>
<owl:Class rdf:about="http://www.wet.protocol#Step"/>
</rdfs:subClassOf>
</owl:Class>
<owl:Class rdf:about="http://www.wet.protocol#Temperature">
<rdfs:subClassOf>
<owl:Class rdf:about="http://www.wet.protocol#Quantity"/>
</rdfs:subClassOf>
</owl:Class>
<owl:Class rdf:about="http://www.wet.protocol#CentifugeTubeRack">
<rdfs:subClassOf>
<owl:Class rdf:about="http://www.wet.protocol#Container"/>
</rdfs:subClassOf>
</owl:Class>
<owl:Class rdf:about="http://www.wet.protocol#Reagent">
<rdfs:subClassOf>
<owl:Class rdf:about="http://www.wet.protocol#Liquid"/>
</rdfs:subClassOf>
</owl:Class>
<owl:Class rdf:about="http://www.wet.protocol#TemperatureControlledStore">
<rdfs:subClassOf>
<owl:Class rdf:about="http://www.wet.protocol#Store"/>
</rdfs:subClassOf>
</owl:Class>
<owl:Class rdf:about="http://www.wet.protocol#Pipette">
<rdfs:subClassOf>
<owl:Class rdf:about="http://www.wet.protocol#VolumetricContainer"/>
</rdfs:subClassOf>
</owl:Class>
<owl:Class rdf:about="http://www.wet.protocol#Container">
<rdfs:subClassOf>
<owl:Class rdf:about="http://www.wet.protocol#Equipment"/>
</rdfs:subClassOf>
</owl:Class>
<owl:Class rdf:about="http://www.wet.protocol#Transfer">
<rdfs:subClassOf rdf:resource="http://www.wet.protocol#Step"/>
</owl:Class>
<owl:Class rdf:about="http://www.wet.protocol#CentrifugeTube">
<rdfs:subClassOf>
<owl:Class rdf:about="http://www.wet.protocol#VolumetricContainer"/>
</rdfs:subClassOf>
</owl:Class>
<owl:Class rdf:about="http://www.wet.protocol#Enzime">
<rdfs:subClassOf>
<owl:Class rdf:about="http://www.wet.protocol#OrganicCompound"/>
</rdfs:subClassOf>
</owl:Class>
<owl:Class rdf:about="http://www.wet.protocol#Dna">
<rdfs:subClassOf>
<owl:Class rdf:about="http://www.wet.protocol#OrganicCompound"/>
</rdfs:subClassOf>
</owl:Class>
<owl:Class rdf:about="http://www.wet.protocol#Protocol"/>
<owl:Class rdf:about="http://www.wet.protocol#Move">
<rdfs:subClassOf rdf:resource="http://www.wet.protocol#Step"/>
</owl:Class>
<owl:Class rdf:about="http://www.wet.protocol#Liquid">
<rdfs:subClassOf>
<owl:Class rdf:about="http://www.wet.protocol#MovableObject"/>
</rdfs:subClassOf>
</owl:Class>
<owl:Class rdf:about="http://www.wet.protocol#Incubate">
<rdfs:subClassOf rdf:resource="http://www.wet.protocol#Step"/>
</owl:Class>
<owl:Class rdf:about="http://www.wet.protocol#MovableObject">
<rdfs:subClassOf>
<owl:Class rdf:about="http://www.wet.protocol#Place"/>
</rdfs:subClassOf>
</owl:Class>
<owl:Class rdf:about="http://www.wet.protocol#VolumetricContainer">
<rdfs:subClassOf rdf:resource="http://www.wet.protocol#Container"/>
</owl:Class>
<owl:Class rdf:about="http://www.wet.protocol#Bench">
<rdfs:subClassOf>
<owl:Class rdf:about="http://www.wet.protocol#Store"/>
</rdfs:subClassOf>
</owl:Class>
<owl:Class rdf:about="http://www.wet.protocol#OrganicCompound">
<rdfs:subClassOf rdf:resource="http://www.wet.protocol#Liquid"/>
<owl:disjointWith rdf:resource="http://www.wet.protocol#Reagent"/>
</owl:Class>
<owl:Class rdf:about="http://www.wet.protocol#Plasmid">
<rdfs:subClassOf rdf:resource="http://www.wet.protocol#OrganicCompound"/>
</owl:Class>
<owl:Class rdf:about="http://www.wet.protocol#Volume">
<rdfs:subClassOf rdf:resource="http://www.wet.protocol#Quantity"/>
</owl:Class>
<owl:Class rdf:about="http://www.wet.protocol#ProvisionAndPlace">
<rdfs:subClassOf>
<owl:Class rdf:about="http://www.wet.protocol#Provision"/>
</rdfs:subClassOf>
</owl:Class>
<owl:Class rdf:about="http://www.wet.protocol#PipetteRack">
<rdfs:subClassOf rdf:resource="http://www.wet.protocol#Container"/>
</owl:Class>
<owl:Class rdf:about="http://www.wet.protocol#Supplier">
<rdfs:subClassOf rdf:resource="http://www.wet.protocol#Place"/>
</owl:Class>
<owl:Class rdf:about="http://www.wet.protocol#TestTube">
<rdfs:subClassOf rdf:resource="http://www.wet.protocol#VolumetricContainer"/>
</owl:Class>
<owl:Class rdf:about="http://www.wet.protocol#Phase">
<rdfs:subClassOf rdf:resource="http://www.wet.protocol#Step"/>
</owl:Class>
<owl:Class rdf:about="http://www.wet.protocol#PipetteAspiration">
<rdfs:subClassOf rdf:resource="http://www.wet.protocol#Step"/>
</owl:Class>
<owl:Class rdf:about="http://www.wet.protocol#Equipment">
<rdfs:subClassOf rdf:resource="http://www.wet.protocol#MovableObject"/>
</owl:Class>
<owl:Class rdf:about="http://www.wet.protocol#NonLiquid">
<rdfs:subClassOf rdf:resource="http://www.wet.protocol#MovableObject"/>
</owl:Class>
<owl:Class rdf:about="http://www.wet.protocol#Time">
<rdfs:subClassOf rdf:resource="http://www.wet.protocol#Quantity"/>
</owl:Class>
<owl:Class rdf:about="http://www.wet.protocol#Provision">
<rdfs:subClassOf rdf:resource="http://www.wet.protocol#Step"/>
</owl:Class>
<owl:Class rdf:about="http://www.wet.protocol#DummyClass"/>
<owl:Class rdf:about="http://www.wet.protocol#Store">
<rdfs:subClassOf rdf:resource="http://www.wet.protocol#MovableObject"/>
</owl:Class>
<owl:ObjectProperty rdf:about="http://www.wet.protocol#provisionedObject">
<rdfs:domain rdf:resource="http://www.wet.protocol#Provision"/>
<rdfs:range rdf:resource="http://www.wet.protocol#MovableObject"/>
</owl:ObjectProperty>
<owl:ObjectProperty rdf:about="http://www.wet.protocol#volumetricContainerVolume">
<rdfs:domain rdf:resource="http://www.wet.protocol#VolumetricContainer"/>
<rdfs:range rdf:resource="http://www.wet.protocol#Volume"/>
</owl:ObjectProperty>
<owl:ObjectProperty rdf:about="http://www.wet.protocol#temperatureControlledStoreTemperature">
<rdfs:subPropertyOf>
<owl:ObjectProperty rdf:about="http://www.wet.protocol#incubateTime"/>
</rdfs:subPropertyOf>
<rdfs:domain rdf:resource="http://www.wet.protocol#TemperatureControlledStore"/>
<rdfs:range rdf:resource="http://www.wet.protocol#Temperature"/>
</owl:ObjectProperty>
<owl:ObjectProperty rdf:about="http://www.wet.protocol#content">
<rdfs:subPropertyOf rdf:resource="http://www.w3.org/2002/07/owl#topObjectProperty"/>
<rdfs:domain rdf:resource="http://www.wet.protocol#Container"/>
<rdfs:range rdf:resource="http://www.wet.protocol#Liquid"/>
</owl:ObjectProperty>
<owl:ObjectProperty rdf:about="http://www.wet.protocol#steps">
<rdfs:domain rdf:resource="http://www.wet.protocol#Transfer"/>
<rdfs:range rdf:resource="http://www.wet.protocol#Step"/>
</owl:ObjectProperty>
<owl:ObjectProperty rdf:about="http://www.wet.protocol#moveDestination">
<rdfs:subPropertyOf>
<owl:ObjectProperty rdf:about="http://www.wet.protocol#preexisting"/>
</rdfs:subPropertyOf>
<rdfs:domain rdf:resource="http://www.wet.protocol#Move"/>
<rdfs:range rdf:resource="http://www.wet.protocol#Place"/>
</owl:ObjectProperty>
<owl:ObjectProperty rdf:about="http://www.wet.protocol#pipetteAspirationSource">
<rdfs:subPropertyOf rdf:resource="http://www.wet.protocol#preexisting"/>
<rdfs:domain rdf:resource="http://www.wet.protocol#PipetteAspiration"/>
<rdfs:range rdf:resource="http://www.wet.protocol#VolumetricContainer"/>
</owl:ObjectProperty>
<owl:ObjectProperty rdf:about="http://www.wet.protocol#pipette">
<rdfs:subPropertyOf rdf:resource="http://www.wet.protocol#preexisting"/>
<rdfs:domain rdf:resource="http://www.wet.protocol#PipetteAspiration"/>
<rdfs:domain rdf:resource="http://www.wet.protocol#PipetteRelease"/>
<rdfs:range rdf:resource="http://www.wet.protocol#Pipette"/>
</owl:ObjectProperty>
<owl:ObjectProperty rdf:about="http://www.wet.protocol#pipetteAspirationVolume">
<rdfs:domain rdf:resource="http://www.wet.protocol#PipetteAspiration"/>
<rdfs:range rdf:resource="http://www.wet.protocol#Volume"/>
</owl:ObjectProperty>
<owl:ObjectProperty rdf:about="http://www.wet.protocol#pipetteReleaseVolume">
<rdfs:domain rdf:resource="http://www.wet.protocol#PipetteRelease"/>
<rdfs:range rdf:resource="http://www.wet.protocol#Volume"/>
</owl:ObjectProperty>
<owl:ObjectProperty rdf:about="http://www.wet.protocol#provisionAndPlaceWhere">
<rdfs:subPropertyOf rdf:resource="http://www.wet.protocol#preexisting"/>
<rdfs:domain rdf:resource="http://www.wet.protocol#ProvisionAndPlace"/>
<rdfs:range rdf:resource="http://www.wet.protocol#Place"/>
</owl:ObjectProperty>
<owl:ObjectProperty rdf:about="http://www.wet.protocol#moveSource">
<rdfs:subPropertyOf rdf:resource="http://www.wet.protocol#preexisting"/>
<rdfs:domain rdf:resource="http://www.wet.protocol#Move"/>
<rdfs:range rdf:resource="http://www.wet.protocol#Place"/>
</owl:ObjectProperty>
<owl:ObjectProperty rdf:about="http://www.wet.protocol#pipetteReleaseDestination">
<rdfs:subPropertyOf rdf:resource="http://www.wet.protocol#preexisting"/>
<rdfs:domain rdf:resource="http://www.wet.protocol#PipetteRelease"/>
<rdfs:range rdf:resource="http://www.wet.protocol#VolumetricContainer"/>
</owl:ObjectProperty>
<owl:ObjectProperty rdf:about="http://www.wet.protocol#incubateTime">
<rdfs:domain rdf:resource="http://www.wet.protocol#Incubate"/>
<rdfs:range rdf:resource="http://www.wet.protocol#Time"/>
</owl:ObjectProperty>
<owl:ObjectProperty rdf:about="http://www.wet.protocol#liquidVolume">
<rdfs:domain rdf:resource="http://www.wet.protocol#Liquid"/>
<rdfs:range rdf:resource="http://www.wet.protocol#Volume"/>
</owl:ObjectProperty>
<owl:ObjectProperty rdf:about="http://www.wet.protocol#minimumVolume">
<rdfs:subPropertyOf rdf:resource="http://www.w3.org/2002/07/owl#topObjectProperty"/>
<rdfs:domain rdf:resource="http://www.wet.protocol#Pipette"/>
<rdfs:range rdf:resource="http://www.wet.protocol#Volume"/>
</owl:ObjectProperty>
<owl:DatatypeProperty rdf:about="http://www.wet.protocol#stepCoordinatesProperty">
<rdfs:range rdf:resource="http://www.w3.org/2001/XMLSchema#string"/>
</owl:DatatypeProperty>
<owl:DatatypeProperty rdf:about="http://www.wet.protocol#dummyClassDataProperty">
<rdfs:domain rdf:resource="http://www.wet.protocol#DummyClass"/>
<rdfs:range rdf:resource="http://www.w3.org/2001/XMLSchema#string"/>
</owl:DatatypeProperty>
<owl:DatatypeProperty rdf:about="http://www.wet.protocol#quantityValue">
<rdfs:subPropertyOf rdf:resource="http://www.w3.org/2002/07/owl#topDataProperty"/>
<rdfs:domain rdf:resource="http://www.wet.protocol#Quantity"/>
<rdfs:range rdf:resource="http://www.w3.org/2001/XMLSchema#decimal"/>
</owl:DatatypeProperty>
<owl:DatatypeProperty rdf:about="http://www.wet.protocol#centrifugeTubeRackColumnsCount">
<rdfs:domain rdf:resource="http://www.wet.protocol#CentifugeTubeRack"/>
<rdfs:range rdf:resource="http://www.w3.org/2001/XMLSchema#int"/>
</owl:DatatypeProperty>
<owl:DatatypeProperty rdf:about="http://www.wet.protocol#plasmidName">
<rdfs:subPropertyOf rdf:resource="http://www.w3.org/2002/07/owl#topDataProperty"/>
<rdfs:domain rdf:resource="http://www.wet.protocol#Plasmid"/>
<rdfs:range rdf:resource="http://www.w3.org/2001/XMLSchema#string"/>
</owl:DatatypeProperty>
<owl:DatatypeProperty rdf:about="http://www.wet.protocol#manufacturer">
<rdfs:subPropertyOf rdf:resource="http://www.w3.org/2002/07/owl#topDataProperty"/>
<rdfs:domain rdf:resource="http://www.wet.protocol#Container"/>
<rdfs:domain rdf:resource="http://www.wet.protocol#Store"/>
<rdfs:range rdf:resource="http://www.w3.org/2001/XMLSchema#string"/>
</owl:DatatypeProperty>
<owl:DatatypeProperty rdf:about="http://www.wet.protocol#quantityUnit">
<rdfs:subPropertyOf rdf:resource="http://www.w3.org/2002/07/owl#topDataProperty"/>
<rdfs:domain rdf:resource="http://www.wet.protocol#Quantity"/>
<rdfs:range rdf:resource="http://www.w3.org/2001/XMLSchema#string"/>
</owl:DatatypeProperty>
<owl:DatatypeProperty rdf:about="http://www.wet.protocol#pipetteRackHookCount">
<rdfs:domain rdf:resource="http://www.wet.protocol#PipetteRack"/>
<rdfs:range rdf:resource="http://www.w3.org/2001/XMLSchema#int"/>
</owl:DatatypeProperty>
<owl:DatatypeProperty rdf:about="http://www.wet.protocol#phasePurpose">
<rdfs:subPropertyOf rdf:resource="http://www.w3.org/2002/07/owl#topDataProperty"/>
<rdfs:domain rdf:resource="http://www.wet.protocol#Phase"/>
<rdfs:range rdf:resource="http://www.w3.org/2001/XMLSchema#string"/>
</owl:DatatypeProperty>
<owl:DatatypeProperty rdf:about="http://www.wet.protocol#fasta">
<rdfs:subPropertyOf rdf:resource="http://www.w3.org/2002/07/owl#topDataProperty"/>
<rdfs:domain rdf:resource="http://www.wet.protocol#Dna"/>
<rdfs:range rdf:resource="http://www.w3.org/2001/XMLSchema#string"/>
</owl:DatatypeProperty>
<owl:DatatypeProperty rdf:about="http://www.wet.protocol#quantityMultiplication">
<rdfs:domain rdf:resource="http://www.wet.protocol#Quantity"/>
<rdfs:range rdf:resource="http://www.w3.org/2001/XMLSchema#int"/>
</owl:DatatypeProperty>
<owl:DatatypeProperty rdf:about="http://www.wet.protocol#protocolCounter">
<rdfs:domain rdf:resource="http://www.wet.protocol#Protocol"/>
<rdfs:range rdf:resource="http://www.w3.org/2001/XMLSchema#int"/>
</owl:DatatypeProperty>
<owl:DatatypeProperty rdf:about="http://www.wet.protocol#centrifugeTubeRackRowsCount">
<rdfs:subPropertyOf rdf:resource="http://www.w3.org/2002/07/owl#topDataProperty"/>
<rdfs:domain rdf:resource="http://www.wet.protocol#CentifugeTubeRack"/>
<rdfs:range rdf:resource="http://www.w3.org/2001/XMLSchema#int"/>
</owl:DatatypeProperty>
<owl:DatatypeProperty rdf:about="http://www.wet.protocol#protocolVersion">
<rdfs:subPropertyOf rdf:resource="http://www.w3.org/2002/07/owl#topDataProperty"/>
<rdfs:domain rdf:resource="http://www.wet.protocol#Protocol"/>
<rdfs:range rdf:resource="http://www.w3.org/2001/XMLSchema#string"/>
</owl:DatatypeProperty>
<owl:NamedIndividual rdf:about="http://www.wet.protocol#provisionAndPlaceCentrifugeTubeRack13">
<www:provisionAndPlaceWhere>
<owl:NamedIndividual rdf:about="http://www.wet.protocol#bench3">
<www:manufacturer>mb</www:manufacturer>
<rdfs:label>Label:bench3</rdfs:label>
<rdf:type rdf:resource="http://www.wet.protocol#Bench"/>
</owl:NamedIndividual>
</www:provisionAndPlaceWhere>
<www:provisionedObject>
<owl:NamedIndividual rdf:about="http://www.wet.protocol#centifugeTubeRack14">
<www:centrifugeTubeRackColumnsCount>8</www:centrifugeTubeRackColumnsCount>
<www:centrifugeTubeRackRowsCount>12</www:centrifugeTubeRackRowsCount>
<www:manufacturer>crm</www:manufacturer>
<rdfs:label>Label:centifugeTubeRack14</rdfs:label>
<rdf:type rdf:resource="http://www.wet.protocol#CentifugeTubeRack"/>
</owl:NamedIndividual>
</www:provisionedObject>
<rdfs:label>Label:provisionAndPlace13</rdfs:label>
<www:stepCoordinatesProperty>5.2</www:stepCoordinatesProperty>
<rdf:type rdf:resource="http://www.wet.protocol#ProvisionAndPlace"/>
</owl:NamedIndividual>
<owl:NamedIndividual rdf:about="http://www.wet.protocol#transform28">
<rdfs:label>Label:transform28</rdfs:label>
<www:stepCoordinatesProperty>14.1</www:stepCoordinatesProperty>
<rdf:type rdf:resource="http://www.wet.protocol#Phase"/>
</owl:NamedIndividual>
<owl:NamedIndividual rdf:about="http://www.wet.protocol#newLeafIndividual40_ofClass_Volume">
<rdfs:label>Label:newLeafIndividual40_ofClass_Volume</rdfs:label>
<rdf:type rdf:resource="http://www.wet.protocol#Volume"/>
</owl:NamedIndividual>
<owl:NamedIndividual rdf:about="http://www.wet.protocol#provisionAndPlaceStopEnzimeCt16">
<www:provisionAndPlaceWhere rdf:resource="http://www.wet.protocol#centifugeTubeRack14"/>
<www:provisionedObject>
<owl:NamedIndividual rdf:about="http://www.wet.protocol#centrifugeTubeStopEnzimeCt21">
<www:content>
<owl:NamedIndividual rdf:about="http://www.wet.protocol#enzimeStop23">
<www:liquidVolume>
<owl:NamedIndividual rdf:about="http://www.wet.protocol#newLeafIndividual24_ofClass_Volume">
<www:quantityMultiplication>-6</www:quantityMultiplication>
<www:quantityUnit>liter</www:quantityUnit>
<www:quantityValue>30</www:quantityValue>
<rdfs:label>Label:newLeafIndividual24_ofClass_Volume</rdfs:label>
<rdf:type rdf:resource="http://www.wet.protocol#Volume"/>
</owl:NamedIndividual>
</www:liquidVolume>
<rdfs:label>Label:enzimeStop23</rdfs:label>
<rdf:type rdf:resource="http://www.wet.protocol#Enzime"/>
</owl:NamedIndividual>
</www:content>
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</owl:NamedIndividual>
</www:volumetricContainerVolume>
<www:manufacturer>mct</www:manufacturer>
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<rdf:type rdf:resource="http://www.wet.protocol#CentrifugeTube"/>
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</www:provisionedObject>
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<rdf:type rdf:resource="http://www.wet.protocol#ProvisionAndPlace"/>
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<www:PipetteRelease rdf:about="http://www.wet.protocol#pipetteReleaseOfStartEnzime">
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<www:Volume rdf:about="http://www.wet.protocol#newLeafIndividual49_ofClass_Volume">
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</www:pipetteReleaseVolume>
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</www:PipetteRelease>
<owl:NamedIndividual rdf:about="http://www.wet.protocol#provisionFridge4">
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<owl:NamedIndividual rdf:about="http://www.wet.protocol#temperatureControlledStore5">
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<owl:NamedIndividual rdf:about="http://www.wet.protocol#newLeafIndividual6_ofClass_Temperature">
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<www:quantityValue>-4</www:quantityValue>
<rdfs:label>Label:newLeafIndividual6_ofClass_Temperature</rdfs:label>
<rdf:type rdf:resource="http://www.wet.protocol#Temperature"/>
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</www:temperatureControlledStoreTemperature>
<www:manufacturer>mf</www:manufacturer>
<rdfs:label>Label:temperatureControlledStore5</rdfs:label>
<rdf:type rdf:resource="http://www.wet.protocol#TemperatureControlledStore"/>
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<rdfs:label>Label:provisionFridge4</rdfs:label>
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<owl:NamedIndividual rdf:about="http://www.wet.protocol#setup1">
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<owl:NamedIndividual rdf:about="http://www.wet.protocol#restrictionDigestOfRecipientPlasmid">
<rdfs:label>Label:phase31</rdfs:label>
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<rdf:type rdf:resource="http://www.wet.protocol#Phase"/>
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<owl:NamedIndividual rdf:about="http://www.wet.protocol#provisionAndPlaceStartEnzimeCt15">
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<owl:NamedIndividual rdf:about="http://www.wet.protocol#centrifugeTubeStartEzimeCt17">
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<owl:NamedIndividual rdf:about="http://www.wet.protocol#enzime19">
<www:liquidVolume>
<owl:NamedIndividual rdf:about="http://www.wet.protocol#newLeafIndividual20_ofClass_Volume">
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<www:quantityUnit>liter</www:quantityUnit>
<www:quantityValue>20</www:quantityValue>
<rdfs:label>Label:newLeafIndividual20_ofClass_Volume</rdfs:label>
<rdf:type rdf:resource="http://www.wet.protocol#Volume"/>
</owl:NamedIndividual>
</www:liquidVolume>
<rdfs:label>Label:enzime19</rdfs:label>
<rdf:type rdf:resource="http://www.wet.protocol#Enzime"/>
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</www:content>
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<owl:NamedIndividual rdf:about="http://www.wet.protocol#newLeafIndividual18_ofClass_Volume">
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<www:quantityUnit>liter</www:quantityUnit>
<www:quantityValue>15</www:quantityValue>
<rdfs:label>Label:newLeafIndividual18_ofClass_Volume</rdfs:label>
<rdf:type rdf:resource="http://www.wet.protocol#Volume"/>
</owl:NamedIndividual>
</www:volumetricContainerVolume>
<www:manufacturer>mct</www:manufacturer>
<rdfs:label>Label:centrifugeTubeStartEzimeCt17</rdfs:label>
<rdf:type rdf:resource="http://www.wet.protocol#CentrifugeTube"/>
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</www:provisionedObject>
<rdfs:label>Label:provisionAndPlaceStartEnzimeCt15</rdfs:label>
<www:stepCoordinatesProperty>7.2</www:stepCoordinatesProperty>
<rdf:type rdf:resource="http://www.wet.protocol#ProvisionAndPlace"/>
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<www:PipetteAspiration rdf:about="http://www.wet.protocol#pipetteAspirationOfStartEnzime">
<rdfs:label>Label:pipetteAspiration42</rdfs:label>
<www:pipetteAspirationVolume>
<www:Volume rdf:about="http://www.wet.protocol#newLeafIndividual43_ofClass_Volume">
<rdfs:label>Label:newLeafIndividual43_ofClass_Volume</rdfs:label>
<www:quantityMultiplication>-6</www:quantityMultiplication>
<www:quantityValue>1</www:quantityValue>
<www:quantityUnit>liter</www:quantityUnit>
</www:Volume>
</www:pipetteAspirationVolume>
<www:pipette>
<owl:NamedIndividual rdf:about="http://www.wet.protocol#pipette10">
<www:minimumVolume>
<owl:NamedIndividual rdf:about="http://www.wet.protocol#newLeafIndividual12_ofClass_Volume">
<www:quantityMultiplication>-6</www:quantityMultiplication>
<www:quantityUnit>liter</www:quantityUnit>
<www:quantityValue>20</www:quantityValue>
<rdfs:label>Label:newLeafIndividual12_ofClass_Volume</rdfs:label>
<rdf:type rdf:resource="http://www.wet.protocol#Volume"/>
</owl:NamedIndividual>
</www:minimumVolume>
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<owl:NamedIndividual rdf:about="http://www.wet.protocol#newLeafIndividual11_ofClass_Volume">
<www:quantityMultiplication>-6</www:quantityMultiplication>
<www:quantityUnit>liter</www:quantityUnit>
<www:quantityValue>200</www:quantityValue>
<rdfs:label>Label:newLeafIndividual11_ofClass_Volume</rdfs:label>
<rdf:type rdf:resource="http://www.wet.protocol#Volume"/>
</owl:NamedIndividual>
</www:volumetricContainerVolume>
<www:manufacturer>mp</www:manufacturer>
<rdfs:label>Label:pipette10</rdfs:label>
<rdf:type rdf:resource="http://www.wet.protocol#Pipette"/>
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</www:pipette>
<www:pipetteAspirationSource rdf:resource="http://www.wet.protocol#centrifugeTubeStartEzimeCt17"/>
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</www:PipetteAspiration>
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<owl:NamedIndividual rdf:about="http://www.wet.protocol#pipetteRack8">
<www:manufacturer>mpr</www:manufacturer>
<www:pipetteRackHookCount>8</www:pipetteRackHookCount>
<rdfs:label>Label:pipetteRack8</rdfs:label>
<rdf:type rdf:resource="http://www.wet.protocol#PipetteRack"/>
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</www:provisionAndPlaceWhere>
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<rdfs:label>Label:provisionAndPlacePipette9</rdfs:label>
<www:stepCoordinatesProperty>6.2</www:stepCoordinatesProperty>
<rdf:type rdf:resource="http://www.wet.protocol#ProvisionAndPlace"/>
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<owl:NamedIndividual rdf:about="http://www.wet.protocol#restrictionDigestOfDonorPlasmid30">
<rdfs:label>Label:restrictionDigestOfDonorPlasmid30</rdfs:label>
<www:stepCoordinatesProperty>10.2</www:stepCoordinatesProperty>
<rdf:type rdf:resource="http://www.wet.protocol#Phase"/>
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<owl:members rdf:parseType="Collection">
<owl:Class rdf:about="http://www.wet.protocol#CentifugeTubeRack"/>
<owl:Class rdf:about="http://www.wet.protocol#Temperature"/>
<owl:Class rdf:about="http://www.wet.protocol#Volume"/>
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<www:ProvisionAndPlace rdf:about="http://www.wet.protocol#provisionAndPlaceMainReactionCentrifugeTube44">
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<www:provisionedObject>
<www:CentrifugeTube rdf:about="http://www.wet.protocol#mainReactionCentrifugeTube45">
<rdfs:label>Label:mainReactionCentrifugeTube45</rdfs:label>
<www:volumetricContainerVolume>
<www:Volume rdf:about="http://www.wet.protocol#newLeafIndividual46_ofClass_Volume">
<rdfs:label>Label:newLeafIndividual46_ofClass_Volume</rdfs:label>
<www:quantityValue>1.5</www:quantityValue>
<www:quantityUnit>liter</www:quantityUnit>
<www:quantityMultiplication>-6</www:quantityMultiplication>
</www:Volume>
</www:volumetricContainerVolume>
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<www:provisionAndPlaceWhere rdf:resource="http://www.wet.protocol#centifugeTubeRack14"/>
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</www:ProvisionAndPlace>
<owl:NamedIndividual rdf:about="http://www.wet.protocol#provisionAndPlacePipetteStand7">
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<www:provisionedObject rdf:resource="http://www.wet.protocol#pipetteRack8"/>
<rdfs:label>Label:provisionAndPlacePipetteStand7</rdfs:label>
<www:stepCoordinatesProperty>4.2</www:stepCoordinatesProperty>
<rdf:type rdf:resource="http://www.wet.protocol#ProvisionAndPlace"/>
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<owl:Class rdf:about="http://www.wet.protocol#Dna"/>
<owl:Class rdf:about="http://www.wet.protocol#Enzime"/>
<owl:Class rdf:about="http://www.wet.protocol#Plasmid"/>
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<owl:NamedIndividual rdf:about="http://www.wet.protocol#topProtocolInstance">
<www:protocolCounter rdf:datatype="http://www.w3.org/2001/XMLSchema#int"
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<www:stepCoordinatesProperty>0.0</www:stepCoordinatesProperty>
<www:protocolVersion>Version 0.1 https://www.addgene.org/protocols/subcloning/</www:protocolVersion>
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<www:Volume rdf:about="http://www.wet.protocol#newLeafIndividual48_ofClass_Volume">
<rdfs:label>Label:newLeafIndividual48_ofClass_Volume</rdfs:label>
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<owl:NamedIndividual rdf:about="http://www.wet.protocol#ligateInsertIntoVector27">
<rdfs:label>Label:ligateInsertIntoVector27</rdfs:label>
<www:stepCoordinatesProperty>13.1</www:stepCoordinatesProperty>
<rdf:type rdf:resource="http://www.wet.protocol#Phase"/>
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<owl:NamedIndividual rdf:about="http://www.wet.protocol#provisionBench2">
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<www:stepCoordinatesProperty>2.2</www:stepCoordinatesProperty>
<rdf:type rdf:resource="http://www.wet.protocol#Provision"/>
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<owl:NamedIndividual rdf:about="http://www.wet.protocol#newLeafIndividual36_ofClass_Volume">
<rdfs:label>Label:newLeafIndividual36_ofClass_Volume</rdfs:label>
<rdf:type rdf:resource="http://www.wet.protocol#Volume"/>
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<owl:NamedIndividual rdf:about="http://www.wet.protocol#dnaPurification26">
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<www:stepCoordinatesProperty>11.1</www:stepCoordinatesProperty>
<rdf:type rdf:resource="http://www.wet.protocol#Phase"/>
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<owl:NamedIndividual rdf:about="http://www.wet.protocol#newLeafIndividual41_ofClass_Volume">
<rdfs:label>Label:newLeafIndividual41_ofClass_Volume</rdfs:label>
<rdf:type rdf:resource="http://www.wet.protocol#Volume"/>
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<owl:NamedIndividual rdf:about="http://www.wet.protocol#newLeafIndividual37_ofClass_Pipette">
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<owl:NamedIndividual rdf:about="http://www.wet.protocol#newLeafIndividual39_ofClass_Volume">
<rdfs:label>Label:newLeafIndividual39_ofClass_Volume</rdfs:label>
<rdf:type rdf:resource="http://www.wet.protocol#Volume"/>
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</www:minimumVolume>
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<owl:NamedIndividual rdf:about="http://www.wet.protocol#newLeafIndividual38_ofClass_Volume">
<rdfs:label>Label:newLeafIndividual38_ofClass_Volume</rdfs:label>
<rdf:type rdf:resource="http://www.wet.protocol#Volume"/>
</owl:NamedIndividual>
</www:volumetricContainerVolume>
<rdfs:label>Label:newLeafIndividual37_ofClass_Pipette</rdfs:label>
<rdf:type rdf:resource="http://www.wet.protocol#Pipette"/>
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<owl:NamedIndividual rdf:about="http://www.wet.protocol#provisionAndPlaceMainCentrifugeTube32">
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<owl:NamedIndividual rdf:about="http://www.wet.protocol#restrictionDigestTube33">
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<owl:NamedIndividual rdf:about="http://www.wet.protocol#newLeafIndividual34_ofClass_Volume">
<www:quantityMultiplication>-3</www:quantityMultiplication>
<www:quantityUnit>liter</www:quantityUnit>
<www:quantityValue>1.5</www:quantityValue>
<rdfs:label>Label:newLeafIndividual34_ofClass_Volume</rdfs:label>
<rdf:type rdf:resource="http://www.wet.protocol#Volume"/>
</owl:NamedIndividual>
</www:volumetricContainerVolume>
<www:manufacturer>eppendorf</www:manufacturer>
<rdfs:label>Label:restrictionDigestTube33</rdfs:label>
<rdf:type rdf:resource="http://www.wet.protocol#CentrifugeTube"/>
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</www:provisionedObject>
<rdfs:label>Label:provisionAndPlaceMainCentrifugeTube32</rdfs:label>
<www:stepCoordinatesProperty>12.2</www:stepCoordinatesProperty>
<rdf:type rdf:resource="http://www.wet.protocol#ProvisionAndPlace"/>
</owl:NamedIndividual>
<owl:NamedIndividual rdf:about="http://www.wet.protocol#isolateFinishedPlasmid29">
<rdfs:label>Label:isolateFinishedPlasmid29</rdfs:label>
<www:stepCoordinatesProperty>19.1</www:stepCoordinatesProperty>
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<owl:NamedIndividual rdf:about="http://www.wet.protocol#dnaDigestion25">
<rdfs:label>Label:DnaDigestion25</rdfs:label>
<www:stepCoordinatesProperty>9.1</www:stepCoordinatesProperty>
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