diff --git a/cg_lims/EPPs/udf/calculate/novaseq_x_denaturation.py b/cg_lims/EPPs/udf/calculate/novaseq_x_denaturation.py
index cdb34a11..90691094 100644
--- a/cg_lims/EPPs/udf/calculate/novaseq_x_denaturation.py
+++ b/cg_lims/EPPs/udf/calculate/novaseq_x_denaturation.py
@@ -53,7 +53,7 @@ def __init__(self, pool: float, denaturation: float, phix: float, naoh: float, b
         )
 
     def get_reagent_list(self):
-        return [self.pool, self.phix, self.naoh, self.buffer]
+        return [self.pool, self.denaturation, self.phix, self.naoh, self.buffer]
 
 
 DENATURATION_VOLUMES = {