diff --git a/cg_lims/EPPs/files/base.py b/cg_lims/EPPs/files/base.py
index 196e9ce3..94e1a27d 100644
--- a/cg_lims/EPPs/files/base.py
+++ b/cg_lims/EPPs/files/base.py
@@ -9,6 +9,7 @@
 from cg_lims.EPPs.files.ont_json_to_udf import parse_ont_report
 from cg_lims.EPPs.files.placement_map.make_96well_placement_map import placement_map
 from cg_lims.EPPs.files.pooling_map.make_pooling_map import pool_map
+from cg_lims.EPPs.files.sample_sheet.create_ont_sample_sheet import create_ont_sample_sheet
 from cg_lims.EPPs.files.sample_sheet.create_sample_sheet import create_sample_sheet
 from cg_lims.EPPs.files.xml_to_udf import parse_run_parameters
 
@@ -26,6 +27,7 @@ def files(ctx):
 files.add_command(hamilton)
 files.add_command(trouble_shoot_kapa)
 files.add_command(make_barcode_csv)
+files.add_command(create_ont_sample_sheet)
 files.add_command(create_sample_sheet)
 files.add_command(parse_run_parameters)
 files.add_command(parse_ont_report)
diff --git a/cg_lims/EPPs/files/sample_sheet/create_ont_sample_sheet.py b/cg_lims/EPPs/files/sample_sheet/create_ont_sample_sheet.py
new file mode 100644
index 00000000..900fe37c
--- /dev/null
+++ b/cg_lims/EPPs/files/sample_sheet/create_ont_sample_sheet.py
@@ -0,0 +1,106 @@
+import logging
+import sys
+from pathlib import Path
+from typing import List
+
+import click
+from cg_lims import options
+from cg_lims.EPPs.files.sample_sheet.models import NanoporeSampleSheetHeader
+from cg_lims.exceptions import LimsError, MissingUDFsError
+from cg_lims.files.manage_csv_files import build_csv
+from cg_lims.get.artifacts import get_artifacts
+from cg_lims.get.samples import get_one_sample_from_artifact
+from genologics.entities import Artifact, Process
+
+LOG = logging.getLogger(__name__)
+
+
+def get_flow_cell_id(artifact: Artifact) -> str:
+    """Return the flow cell ID of an artifact from the connected container's name."""
+    container_name: str = artifact.container.name
+    if not container_name:
+        raise MissingUDFsError(f"Artifact {artifact.name} is missing a flow cell ID!")
+    return container_name
+
+
+def get_flow_cell_type(process: Process) -> str:
+    """Return the flow cell type used for the sequencing run."""
+    if not process.udf.get("ONT Flow Cell Type"):
+        raise MissingUDFsError(f"Sample sheet generation requires a flow cell type!")
+    return process.udf.get("ONT Flow Cell Type")
+
+
+def get_sample_id(artifact: Artifact) -> str:
+    """Return the sample ID for a given artifact."""
+    return get_one_sample_from_artifact(artifact=artifact).id
+
+
+def get_experiment_name(process: Process) -> str:
+    """Return the experiment name used for the sequencing run."""
+    if not process.udf.get("Experiment Name"):
+        raise MissingUDFsError(f"Sample sheet generation requires an experiment name!")
+    return process.udf.get("Experiment Name")
+
+
+def get_kit(process: Process) -> str:
+    """Return the prep kits used, in the format required for sample sheet generation."""
+    library_kit: str = process.udf.get("ONT Prep Kit")
+    expansion_kit: str = process.udf.get("ONT Expansion Kit")
+    if not library_kit:
+        raise MissingUDFsError("Sample sheet generation requires a library kit name!")
+    if expansion_kit:
+        library_kit = f"{library_kit} {expansion_kit}"
+    return library_kit
+
+
+def get_header() -> List[str]:
+    """Return the header of the sample sheet."""
+    return [
+        NanoporeSampleSheetHeader.FLOW_CELL_ID,
+        NanoporeSampleSheetHeader.FLOW_CELL_PROD_CODE,
+        NanoporeSampleSheetHeader.SAMPLE_ID,
+        NanoporeSampleSheetHeader.EXPERIMENT_ID,
+        NanoporeSampleSheetHeader.KIT,
+    ]
+
+
+def get_row(artifact: Artifact, process: Process) -> List[str]:
+    """Return the sample sheet row of one sample."""
+    return [
+        get_flow_cell_id(artifact=artifact),
+        get_flow_cell_type(process=process),
+        get_sample_id(artifact=artifact),
+        get_experiment_name(process=process),
+        get_kit(process=process),
+    ]
+
+
+def get_sample_sheet_content(process: Process) -> List[List[str]]:
+    """Return the sample sheet content."""
+    rows: List = []
+    artifacts: List[Artifact] = get_artifacts(process=process)
+    for artifact in artifacts:
+        rows.append(get_row(artifact=artifact, process=process))
+    return rows
+
+
+@click.command()
+@options.file_placeholder(help="File placeholder name.")
+@click.pass_context
+def create_ont_sample_sheet(ctx, file: str):
+    """Create an Oxford Nanopore sample sheet .csv file from an 'ONT Start Sequencing' step."""
+    LOG.info(f"Running {ctx.command_path} with params: {ctx.params}")
+
+    process: Process = ctx.obj["process"]
+
+    try:
+        header: List[str] = get_header()
+        sample_sheet_content: List[List[str]] = get_sample_sheet_content(process=process)
+        file_path: Path = Path(f"{file}_sample_sheet_{get_experiment_name(process=process)}.csv")
+        build_csv(rows=sample_sheet_content, headers=header, file=file_path)
+        message: str = "The sample sheet was successfully generated."
+        LOG.info(message)
+        click.echo(message)
+    except LimsError as e:
+        LOG.error(e.message)
+        sys.exit(e.message)
diff --git a/cg_lims/EPPs/files/sample_sheet/models.py b/cg_lims/EPPs/files/sample_sheet/models.py
index 07dc29db..d3e9c12c 100644
--- a/cg_lims/EPPs/files/sample_sheet/models.py
+++ b/cg_lims/EPPs/files/sample_sheet/models.py
@@ -178,3 +178,11 @@ def get_bclconversion_data_row(self) -> str:
         if self.run_settings.barcode_mismatches:
             line = line + f",{self.barcode_mismatch_index_1},{self.barcode_mismatch_index_2}"
         return line + "\n"
+
+
+class NanoporeSampleSheetHeader(StrEnum):
+    FLOW_CELL_ID: str = "flow_cell_id"
+    FLOW_CELL_PROD_CODE: str = "flow_cell_product_code"
+    SAMPLE_ID: str = "sample_id"
+    EXPERIMENT_ID: str = "experiment_id"
+    KIT: str = "kit"