diff --git a/cg_lims/EPPs/udf/calculate/constants.py b/cg_lims/EPPs/udf/calculate/constants.py
index 9a3f7d2a..0d249e5c 100644
--- a/cg_lims/EPPs/udf/calculate/constants.py
+++ b/cg_lims/EPPs/udf/calculate/constants.py
@@ -20,7 +20,7 @@ class FlowCellSize(IntEnum):
 class FlowCellLaneVolumes10B(FloatEnum):
     """The recommended volume of reagents per 10B flow cell lane. All values are in ul."""
 
-    POOL_VOLUME: float = 34
+    POOL_VOLUME: float = 35
     PHIX_VOLUME: float = 1
     NAOH_VOLUME: float = 8.5
     BUFFER_VOLUME: float = 127.5
@@ -29,7 +29,7 @@ class FlowCellLaneVolumes10B(FloatEnum):
 class FlowCellLaneVolumes15B(FloatEnum):
     """The recommended volume of reagents per 1.5B flow cell lane. All values are in ul."""
 
-    POOL_VOLUME: float = 34
+    POOL_VOLUME: float = 35
     PHIX_VOLUME: float = 1
     NAOH_VOLUME: float = 8.5
     BUFFER_VOLUME: float = 127.5
@@ -38,7 +38,7 @@ class FlowCellLaneVolumes15B(FloatEnum):
 class FlowCellLaneVolumes25B(FloatEnum):
     """The recommended volume of reagents per 25B flow cell lane. All values are in ul."""
 
-    POOL_VOLUME: float = 56
+    POOL_VOLUME: float = 57
     PHIX_VOLUME: float = 1.6
     NAOH_VOLUME: float = 14
     BUFFER_VOLUME: float = 210