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configExample.json
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{
"_comment": "SBATCH configuration",
"slurm_header": {
"time": "12:00:00",
"threads": "8",
"qos": "normal",
"job_prefix": "MLST",
"project": "production",
"type": "core"
},
"regex": {
"mail_recipient": "[email protected]",
"_comment": "File finding patterns. Only single capture group accepted (for reverse/forward identifier)",
"file_pattern": "\\w{8,12}_\\w{8,10}(?:-\\d+)*_L\\d_(?:R)*(\\d{1}).fastq.gz",
"_comment": "Organisms recognized enough to be considered stable",
"verified_organisms": []
},
"_comment": "Folders",
"folders": {
"_comment": "Root folder for ALL output",
"results": "/tmp/MLST/results/",
"_comment": "Report collection folder",
"reports": "/tmp/MLST/reports/",
"_comment": "Log file position and name",
"log_file": "/tmp/microsalt.log",
"_comment": "Root folder for input fasta sequencing data",
"seqdata": "/tmp/projects/",
"_comment": "ST profiles. Each ST profile file under 'profiles' have an identicial folder under references",
"profiles": "/tmp/MLST/references/ST_profiles",
"_comment": "ST folder. Each references sub-folder contains the .tfa file for each locus of the organism",
"references": "/tmp/MLST/references/ST_loci",
"_comment": "Resistances. Commonly from resFinder",
"resistances": "/tmp/MLST/references/resistances",
"_comment": "Download path for NCBI genomes, for alignment usage",
"genomes": "/tmp/MLST/references/genomes",
"_comment": "PubMLST credentials",
"pubmlst_credentials": "/tmp/MLST/credentials"
},
"_comment": "Database/Flask configuration",
"database": {
"SQLALCHEMY_DATABASE_URI": "sqlite:////tmp/microsalt.db",
"SQLALCHEMY_TRACK_MODIFICATIONS": "False",
"DEBUG": "True"
},
"_comment": "Thresholds for Displayed results",
"threshold": {
"_comment": "Typing thresholds",
"mlst_id": 100,
"mlst_novel_id": 99.5,
"mlst_span": 90,
"motif_id": 97,
"motif_span": 90,
"_comment": "Quality Control thresholds",
"total_reads_warn": 75,
"total_reads_fail": 70,
"NTC_total_reads_warn": 10,
"NTC_total_reads_fail": 20,
"mapped_rate_warn": 50,
"mapped_rate_fail": 30,
"duplication_rate_warn": 20,
"duplication_rate_fail": 80,
"insert_size_warn": 140,
"insert_size_fail": 100,
"average_coverage_warn": 100,
"average_coverage_fail": 10,
"bp_10x_warn": 85,
"bp_10x_fail": 75,
"bp_30x_warn": 70,
"bp_50x_warn": 50,
"bp_100x_warn": 20
},
"_comment": "Genologics temporary configuration file",
"genologics": {
"baseuri": "https://lims.facility.se/",
"username": "limsuser",
"password": "mypassword"
},
"_comment": "PubMLST credentials",
"pubmlst": {
"client_id": "",
"client_secret": ""
}
}