Hardware reference
Mach3Manual: Editing Hardware Reference
The dOPM system described in this manual is based on an Eclipse Ti2-E which is the motorised version of the Eclipse Ti2-E series.
In particular each system comes with a hardcopy of the Nikon Eclipse Ti2-E manual. Also check the online resources:
https://www.microscope.healthcare.nikon.com/products/inverted-microscopes/eclipse-ti2-series https://www.microscope.healthcare.nikon.com/resources
| photo of system at IRB in 2022 | photo of dOPM optics at IRB in 2022 |
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| Click Device Manager in Device Tab | Device Manager |
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Diagram from NIS-Elements showing how the hardware components are connected.
Comment TS: Could you maybe include how to get to this panel in the software?
Each dOPM system should be using the same laser lines and emission filters and dichroics. Bear in mind that the Ti2 frame may have some extra dichroics in the upper and lower turrets.
To confirm which filters are in use, short of physically checking the labels on the filters, you can check the configuration of filter wheels in NIS-elements. When the system was first installed the filters should have been manually entered into the hardware configuration definition within NIS-elements.
From the screenshot you can see an upper turret, lower turret. Also, 'Filter A' which refers to the filters that are in the Sutter filter wheel use in the dOPM detection path.
Comment TS: a brief explanation about the function of the lower and upper filter would be nice at this section (or a hyperlink to the respective page)
For the Nikon frame there is a lower and upper filter cube turret. For future upgrades there is a mirror in the upper turret. For the lower turret one of the following filter cubes is removed (probably the DAPI - check system setup) to allow clear path to dOPM optics.
Upper and lower turrets for filter wheel - we only use the lower one in the current implementation. For the upper turret there should be no filters or mirrors in the current implementation.
Called the 'Stage up kit' the version of the Nikon Eclipse Ti2-E frame we have has this which allows for an additional filter cube wheel to combine more light-paths. For our purposes this is for future upgrades and not currently being used.
https://downloads.microscope.healthcare.nikon.com/phase4/literature/Brochures/Ti2_2CE-MPOH-1.pdf
Comment TS: is it possible to make a table here instead to provide an easier overview?
| Lower Filter Cube Turret | |
|---|---|
| Cube name | Description |
| MXR00303 DAPI-5060C Filter Cube | Excitation: EX 377/50 Dicroic Mirror DM 409 Barrier Filter: BA 447/60 |
| MXR00304 CFP-2432C Filter Cube | Excitation: EX 377/50 Dicroic Mirror DM 409 Barrier Filter: BA 447/60 |
| MXR00306 GFP-1828A Filter Cube | Excitation: EX 377/50 Dicroic Mirror DM 409 Barrier Filter: BA 447/60 |
| MXR00310 YFP-2427B Filter Cube | Excitation: EX 377/50 Dicroic Mirror DM 409 Barrier Filter: BA 447/60 |
| MXR00313 TXRED-4040C Filter Cube | Excitation: EX 377/50 Dicroic Mirror DM 409 Barrier Filter: BA 447/60 |
| MXR00315 Cy5-4040C Filter Cube | Excitation: EX 377/50 Dicroic Mirror DM 409 Barrier Filter: BA 447/60 |
| Upper Filter Cube Turret | |
| 1CRP8610 | P861/100/254, High quality 100% mirror for switching optical pathways (25x40mm) |
As of 2023/02/26 here is a snapshot of filters that should be in each systems Sutter filter wheel.
Note that each system might have extra or different filters and these can be accessed via the nis elements software as shown in the screenshot above.
| Imperial | |
|---|---|
| Filter Matched Ex (nm) | Em (nm) |
| 445 | FF02-483/32 |
| 488 | FF03-525/50 |
| 515 | FF01-542/20 |
| 561 | FF01-600/52 |
| 642 | FF01-698/70 |
| Edinburgh | |
| Filter Matched Ex (nm) | Em (nm) |
| 445 | FF01-483/32 |
| 445 | FF01-482/18 |
| 488 | FF01-525/45 |
| 515 | FF01-542/20 |
| 561 | FF01-609/54 |
| 642 | FF01-697/58 |
| Crick | |
| Filter Matched Ex (nm) | Em (nm) |
| 445 | FF01-483/32 |
| 445 | FF01-482/18 |
| 488 | FF01-525/45 |
| 515 | FF01-542/20 |
| 561 | FF01-609/54 |
| 642 | FF01-697/58 |
| 642 | FF01-698/70 |
| IRB | |
| Filter Matched Ex (nm) | Em (nm) |
| 445 | FF01-483/32 |
| 445 | FF01-482/18 |
| 488 | FF01-525/45 |
| 515 | FF01-542/20 |
| 561 | FF01-609/54 |
| 642 | FF01-697/58 |
| ICR | |
| Filter Matched Ex (nm) | Em (nm) |
| 445 | FF01-483/32 |
| 445 | FF01-482/18 |
| 488 | FF01-525/45 |
| 515 | FF01-542/20 |
| 561 | FF01-609/54 |
| 642 | FF01-697/58 |