Troubleshooting

stage not connecting in nis-elements

Disconnect stage USB connection and power cycle and restart NIS-elements

If stage is powercycled - normally we leave the stage on always - then follow these steps to initiate the stage

lasers not turning on

laser power seems to have dropped

nis-elements optical configurations not found

computer won't boot

strange background noise

alignment of dOPM optics not right

my prefind is not working

stage coordinates are not right

dOPM dual view bead data cannot be registered

my position list is not correct

Has the sample been displaced by contact with the microscope objective.

My sample is unhappy

• is my imaging media OK
• is the CO2 humidifier empty/topped up
• is the CO2 5% and stable - use meter
• is temperature stable
• is there an infection
• phototoxicity

A way to rule out dOPM is to run duplicate assays and keep in your incubator

Also beneficial to collect low res widefield images of wells to get an overview of typical growth