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Localized Reconstruction

Localized Reconstruction is a general method for the localized three-dimensional reconstruction of substructures bound to a larger particle. After determining the particle orientations via conventional methods, local areas corresponding to the subunits ('subparticles') can be extracted and treated as single particles.

If you have any questions, please don't hesitate to contact us at [email protected].

Current version

These instructions are for version 1.2.0.

References

If localized reconstruction is useful in your work, please cite:

Ilca SL, Kotecha A, Sun X, Poranen MM, Stuart DI & Huiskonen JT (2015). Localized reconstruction of subunits from electron cryomicroscopy images of macromolecular complexes. Nat Commun 6, 8843.

If you use partial signal subtraction as part of this method, please cite also Bai et al. 2015 eLife.

If you have used Scipion to run localized reconstruction, please cite also de la Rosa-Trevin et al. 2016 J Struct Biol.

Getting started

• How to install

• How to run

• Frequently asked questions

Acknowledgements

The authors acknowledge funding from the European Research Council under the European Union’s Horizon 2020 research and innovation programme (649053) and from the Wellcome Trust PhD Programme in Structural Biology.