diff --git a/zUMIs-bbmap.R b/zUMIs-bbmap.R index f89265f..b90a0dc 100644 --- a/zUMIs-bbmap.R +++ b/zUMIs-bbmap.R @@ -14,16 +14,16 @@ outbam <- paste0(inp$out_dir,"/",inp$project,".filtered.tagged.Aligned.out.bam") starbam <- paste0(inp$out_dir,"/",inp$project,".filtered.tagged.Aligned.STAR.bam") bbbam <- paste0(inp$out_dir,"/",inp$project,".filtered.tagged.Aligned.bbmap.sam.gz") fastqfile <- paste0(inp$out_dir,"/",inp$project,".filtered.tagged.cDNA.fastq.gz") -fasta <- paste0(inp$reference$STAR_index,"/Genome") -## rename!! -fasta <- "/data/ngs/genomes/Human/hg38/Homo_sapiens.GRCh38.dna.primary_assembly.fa" +#fasta <- paste0(inp$reference$STAR_index,"/Genome") +#fasta <- "/data/ngs/genomes/Human/hg38/Homo_sapiens.GRCh38.dna.primary_assembly.fa" +fasta <- inp$reference$bbmap_fasta ###### make Fastq system(paste("mv",outbam,starbam)) #system(paste0("picard SamToFastq I=",starbam," O=",fastqfile)) # ###### run BBmap -bb_cmd <- paste0(samtools," fastq ",starbam, +bb_cmd <- paste0(samtools," fastq ",starbam, " | bbmap local=t maxindel=200000 nhtag=t nmtag=t ambiguous=best ordered=t int=f out=stdout.sam in=stdin.fq", " out=",bbbam, " threads=",cores,