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100519MeasureIntesity.cp
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CellProfiler Pipeline: http://www.cellprofiler.org
Version:1
SVNRevision:9777
LoadImages:[module_num:1|svn_version:\'9777\'|variable_revision_number:5|show_window:False|notes:\x5B\x5D]
File type to be loaded:individual images
File selection method:Text-Exact match
Number of images in each group?:3
Type the text that the excluded images have in common:Do not use
Analyze all subfolders within the selected folder?:No
Input image file location:Default Input Folder\x7CNone
Check image sets for missing or duplicate files?:Yes
Group images by metadata?:No
Exclude certain files?:No
Specify metadata fields to group by:
Text that these images have in common (case-sensitive):w2
Name this loaded image:GFP
Position of this image in each group:1
Extract metadata from where?:None
Regular expression that finds metadata in the file name:^(?P<Plate>.*)_(?P<Well>\x5BA-P\x5D\x5B0-9\x5D{2})_s(?P<Site>\x5B0-9\x5D)
Type the regular expression that finds metadata in the subfolder path:.*\x5B\\\\/\x5D(?P<Date>.*)\x5B\\\\/\x5D(?P<Run>.*)$
MeasureImageIntensity:[module_num:2|svn_version:\'9604\'|variable_revision_number:2|show_window:False|notes:\x5B\x5D]
Select the image to measure:GFP
Measure the intensity only from areas enclosed by objects?:No
Select the input objects:None
ExportToSpreadsheet:[module_num:3|svn_version:\'9660\'|variable_revision_number:6|show_window:False|notes:\x5B\x5D]
Select or enter the column delimiter:Comma (",")
Prepend the output file name to the data file names?:Yes
Add image metadata columns to your object data file?:No
Limit output to a size that is allowed in Excel?:No
Select the columns of measurements to export?:Yes
Calculate the per-image mean values for object measurements?:No
Calculate the per-image median values for object measurements?:No
Calculate the per-image standard deviation values for object measurements?:No
Output file location:Default Output Folder\x7CNone
Export all measurements?:Yes
Press button to select measurements to export:Image\x7CFileName_GFP,Image\x7CIntensity_MinIntensity_GFP,Image\x7CIntensity_TotalIntensity_GFP,Image\x7CIntensity_TotalArea_GFP,Image\x7CIntensity_MeanIntensity_GFP,Image\x7CIntensity_MaxIntensity_GFP,Image\x7CIntensity_MedianIntensity_GFP
Data to export:Do not use
Combine these object measurements with those of the previous object?:No
File name:DATA.csv
Use the object name for the file name?:Yes