3_variant_call_and_deriving_consensus.ipynb

{
 "cells": [
  {
   "cell_type": "code",
   "execution_count": 1,
   "metadata": {},
   "outputs": [],
   "source": [
    "%%bash\n",
    "mkdir ./samtools_out"
   ]
  },
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": [
    "## these steps are needed to open bam file in IGV :\n",
    "- IGV takes sorted indexed `bam` file as an input"
   ]
  },
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": [
    "#### 1. convert a file from `sam` into `bam` format : \n",
    "\n",
    "- `-b` output is bam\n",
    "- `-S` input is sam\n",
    "- `-o` output file name"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 1,
   "metadata": {},
   "outputs": [],
   "source": [
    "%%bash\n",
    "samtools view \\\n",
    "-b -S -o \\\n",
    "./samtools_out/saratov_map_unpaired.bam \\\n",
    "./bowtie_out/saratov_map_unpaired.sam "
   ]
  },
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": [
    "#### 2. sort reads by their genome positions :"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 2,
   "metadata": {},
   "outputs": [],
   "source": [
    "%%bash\n",
    "samtools sort ./samtools_out/saratov_map_unpaired.bam > ./samtools_out/saratov_map_unpaired_sorted.bam"
   ]
  },
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": [
    "#### 3. remove pcr duplicates from sorted file :"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 3,
   "metadata": {},
   "outputs": [
    {
     "name": "stderr",
     "output_type": "stream",
     "text": [
      "[bam_rmdup_core] processing reference AF409138.1...\n"
     ]
    }
   ],
   "source": [
    "%%bash\n",
    "samtools rmdup  ./samtools_out/saratov_map_unpaired_sorted.bam ./samtools_out/saratov_map_unpaired_sorted_rmdup.bam"
   ]
  },
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": [
    "#### 4. indexing sorted file : "
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 4,
   "metadata": {},
   "outputs": [],
   "source": [
    "%%bash\n",
    "samtools index ./samtools_out/saratov_map_unpaired_sorted_rmdup.bam"
   ]
  },
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": [
    "#### now you can open file `saratov_map_unpaired_sorted_rmdup.bam` with IGV to visualize mapping reads and variants :_"
   ]
  },
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": [
    "![igv](./pictures/igv_example.png)"
   ]
  },
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": [
    "we can also import `sam` file into Ugene (it sorts, creates index and makes file `ugenedb`, which can be opened in Ugene afterwards) to visualize the mapped reads :"
   ]
  },
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": [
    "![ugene](./pictures/ugene_example.png)"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 3,
   "metadata": {},
   "outputs": [
    {
     "name": "stdout",
     "output_type": "stream",
     "text": [
      "1_trim_crop_and_trail_32_1P.fastq\n",
      "1_trim_crop_and_trail_32_1U.fastq\n",
      "1_trim_crop_and_trail_32_2P.fastq\n",
      "1_trim_crop_and_trail_32_2U.fastq\n",
      "2_trim_crop_and_trail_32_2P.fastq\n",
      "SUD-Saratov_S1_L001_R1_001.fastq\n",
      "SUD-Saratov_S1_L001_R2_001.fastq\n",
      "input_data.rar\n",
      "merged_document.aln\n",
      "ref_AF409138.1.fasta\n",
      "ref_AF409138.1.fasta.fai\n",
      "saratov_map_on_AF_consensus.fa\n"
     ]
    }
   ],
   "source": [
    "%%bash\n",
    "ls ./input_data/\n"
   ]
  },
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": [
    "####  6. calculating variants\n",
    "- `mpileup` calculates likelihoods of the variants\n",
    "- we can skip indels by `--skip-indels` flag (can be denoted as `-I` for short)\n",
    "- `--BCF` is the output option (output in binary call format) and may be `-g` for short\n",
    "- `--fasta` denots reference file format, can be `-f` for short"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 6,
   "metadata": {},
   "outputs": [
    {
     "name": "stderr",
     "output_type": "stream",
     "text": [
      "[mpileup] 1 samples in 1 input files\n",
      "<mpileup> Set max per-file depth to 8000\n"
     ]
    }
   ],
   "source": [
    "%%bash\n",
    "samtools mpileup --BCF \\\n",
    "--fasta ./input_data/ref_AF409138.1.fasta \\\n",
    "./samtools_out/saratov_map_unpaired_sorted_rmdup.bam > ./samtools_out/saratov_vars.bcf"
   ]
  },
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": [
    "#### 7. calling variants. conventional variant\n",
    "- `-c`, `--consensus-caller`  the original samtools/bcftools calling method (conflicts with -m)\n",
    "- `-v`, `--variants-only` output variant sites only\n",
    "- `-V`, `--skip-variants snps|indels` skip indel/SNP sites\n",
    "- `--ploidy-file` path to the file which denotes set of chromosomes, if not set, all samples are considered diploid. It's better to set it to improve variants call quality (use to get help : `bcftools call --ploidy ?`)"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 6,
   "metadata": {},
   "outputs": [],
   "source": [
    "%%bash\n",
    "bcftools call \\\n",
    "--consensus-caller \\\n",
    "--variants-only \\\n",
    "--ploidy-file ./samtools_out/ploid.txt \\\n",
    "./samtools_out/saratov_vars.bcf > ./samtools_out/saratov_vars.vcf"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": null,
   "metadata": {},
   "outputs": [],
   "source": []
  },
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": [
    "#### from here we can parse `vcf` to explore the difference from the reference"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 13,
   "metadata": {},
   "outputs": [],
   "source": [
    "import vcf"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 12,
   "metadata": {
    "scrolled": true
   },
   "outputs": [
    {
     "name": "stdout",
     "output_type": "stream",
     "text": [
      "Record(CHROM=AF409138.1, POS=502, REF=A, ALT=[G])\n",
      "47\n",
      "[0, 0, 14, 26]\n",
      "{'DP': 47, 'VDB': 0.242997, 'SGB': -0.693145, 'MQSB': 0.20277, 'MQ0F': 0.340426, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 14, 26], 'MQ': 1, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=759, REF=C, ALT=[T])\n",
      "24\n",
      "[0, 0, 7, 15]\n",
      "{'DP': 24, 'VDB': 0.001429, 'SGB': -0.692562, 'MQSB': 0.760398, 'MQ0F': 0.166667, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 7, 15], 'MQ': 1, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=5386, REF=A, ALT=[G])\n",
      "10\n",
      "[0, 0, 1, 7]\n",
      "{'DP': 10, 'VDB': 0.020653, 'SGB': -0.651104, 'MQSB': 1.0, 'MQ0F': 0.1, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 1, 7], 'MQ': 21, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=5498, REF=T, ALT=[G])\n",
      "21\n",
      "[0, 0, 13, 6]\n",
      "{'DP': 21, 'VDB': 0.650357, 'SGB': -0.69168, 'MQSB': 0.850016, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 13, 6], 'MQ': 39, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=5534, REF=A, ALT=[G])\n",
      "16\n",
      "[0, 0, 4, 3]\n",
      "{'DP': 16, 'VDB': 0.0098501, 'SGB': -0.636426, 'MQSB': 0.810265, 'MQ0F': 0.0625, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 4, 3], 'MQ': 20, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=5536, REF=T, ALT=[C])\n",
      "7\n",
      "[0, 0, 4, 2]\n",
      "{'DP': 7, 'VDB': 0.00688729, 'SGB': -0.616816, 'MQSB': 1.0, 'MQ0F': 0.142857, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 4, 2], 'MQ': 19, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=5562, REF=G, ALT=[A])\n",
      "4\n",
      "[0, 0, 3, 0]\n",
      "{'DP': 4, 'VDB': 0.0221621, 'SGB': -0.511536, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 3, 0], 'MQ': 19, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=5574, REF=ATTTTTT, ALT=[ATTTTTTT])\n",
      "7\n",
      "[0, 0, 5, 2]\n",
      "{'INDEL': True, 'IDV': 6, 'IMF': 0.857143, 'DP': 7, 'VDB': 0.0408989, 'SGB': -0.636426, 'MQSB': 1.0, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 5, 2], 'MQ': 25, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=5657, REF=G, ALT=[A, C])\n",
      "14\n",
      "[0, 0, 1, 10]\n",
      "{'DP': 14, 'VDB': 0.84546, 'SGB': -0.676189, 'MQSB': 1.0, 'MQ0F': 0.214286, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 1, 10], 'MQ': 31, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=5735, REF=C, ALT=[T])\n",
      "6\n",
      "[0, 0, 2, 4]\n",
      "{'DP': 6, 'VDB': 0.0769344, 'SGB': -0.616816, 'MQSB': 0.875, 'MQ0F': 0.333333, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 2, 4], 'MQ': 11, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=5737, REF=C, ALT=[T])\n",
      "6\n",
      "[0, 0, 2, 4]\n",
      "{'DP': 6, 'VDB': 0.0697235, 'SGB': -0.616816, 'MQSB': 0.875, 'MQ0F': 0.333333, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 2, 4], 'MQ': 11, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=5762, REF=C, ALT=[T, G])\n",
      "11\n",
      "[0, 0, 3, 8]\n",
      "{'DP': 11, 'VDB': 0.990565, 'SGB': -0.676189, 'MQSB': 0.994805, 'MQ0F': 0.181818, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 3, 8], 'MQ': 25, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=5775, REF=C, ALT=[T, G])\n",
      "11\n",
      "[0, 0, 0, 10]\n",
      "{'DP': 11, 'VDB': 0.00835326, 'SGB': -0.670168, 'MQ0F': 0.181818, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 0, 10], 'MQ': 26, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=5819, REF=T, ALT=[G])\n",
      "7\n",
      "[0, 0, 4, 3]\n",
      "{'DP': 7, 'VDB': 0.00748664, 'SGB': -0.636426, 'MQSB': 0.202566, 'MQ0F': 0.142857, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 4, 3], 'MQ': 24, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=5886, REF=T, ALT=[C])\n",
      "9\n",
      "[0, 0, 4, 4]\n",
      "{'DP': 9, 'VDB': 0.0330611, 'SGB': -0.651104, 'MQSB': 0.62023, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 4, 4], 'MQ': 31, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=7772, REF=A, ALT=[G])\n",
      "26\n",
      "[0, 0, 3, 17]\n",
      "{'DP': 26, 'VDB': 0.595873, 'SGB': -0.692067, 'MQSB': 0.95083, 'MQ0F': 0.0384615, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 3, 17], 'MQ': 27, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=7773, REF=T, ALT=[C, A])\n",
      "28\n",
      "[0, 0, 3, 16]\n",
      "{'DP': 28, 'VDB': 0.408857, 'SGB': -0.69168, 'MQSB': 0.986036, 'MQ0F': 0.0357143, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 3, 16], 'MQ': 26, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=8466, REF=C, ALT=[A])\n",
      "15\n",
      "[0, 0, 3, 11]\n",
      "{'DP': 15, 'VDB': 0.17229, 'SGB': -0.686358, 'MQSB': 0.334895, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 3, 11], 'MQ': 38, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=9540, REF=T, ALT=[C])\n",
      "20\n",
      "[0, 0, 10, 5]\n",
      "{'DP': 20, 'VDB': 0.36186, 'SGB': -0.688148, 'MQSB': 0.728422, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 10, 5], 'MQ': 38, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=9597, REF=C, ALT=[T])\n",
      "20\n",
      "[0, 0, 10, 7]\n",
      "{'DP': 20, 'VDB': 9.85389e-05, 'SGB': -0.690438, 'MQSB': 0.562035, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 10, 7], 'MQ': 39, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=9614, REF=C, ALT=[T])\n",
      "14\n",
      "[0, 0, 3, 11]\n",
      "{'DP': 14, 'VDB': 0.0322662, 'SGB': -0.686358, 'MQSB': 0.374629, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 3, 11], 'MQ': 40, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=12429, REF=C, ALT=[A])\n",
      "36\n",
      "[0, 0, 9, 21]\n",
      "{'DP': 36, 'VDB': 0.553623, 'SGB': -0.693097, 'MQSB': 0.609176, 'MQ0F': 0.0277778, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 9, 21], 'MQ': 36, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=12523, REF=G, ALT=[A])\n",
      "24\n",
      "[0, 0, 20, 4]\n",
      "{'DP': 24, 'VDB': 0.00274279, 'SGB': -0.692831, 'MQSB': 0.953134, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 20, 4], 'MQ': 38, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=12568, REF=C, ALT=[T])\n",
      "15\n",
      "[0, 0, 9, 2]\n",
      "{'DP': 15, 'VDB': 0.0355294, 'SGB': -0.676189, 'MQSB': 0.555556, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 9, 2], 'MQ': 37, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=12583, REF=A, ALT=[G])\n",
      "19\n",
      "[0, 0, 2, 12]\n",
      "{'DP': 19, 'VDB': 0.00517783, 'SGB': -0.686358, 'MQSB': 0.791667, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 2, 12], 'MQ': 34, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=12633, REF=C, ALT=[A])\n",
      "22\n",
      "[0, 2, 8, 9]\n",
      "{'DP': 22, 'VDB': 0.269298, 'SGB': -0.690438, 'RPB': 1.0, 'MQB': 0.529412, 'MQSB': 1.0, 'BQB': 0.941176, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 2, 8, 9], 'MQ': 38, 'FQ': -999.0, 'PV4': [0.48538, 1.0, 0.253622, 1.0]}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=12723, REF=G, ALT=[A])\n",
      "13\n",
      "[0, 0, 3, 8]\n",
      "{'DP': 13, 'VDB': 0.684373, 'SGB': -0.676189, 'MQSB': 0.954207, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 3, 8], 'MQ': 37, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=14451, REF=C, ALT=[T, G])\n",
      "32\n",
      "[0, 0, 17, 14]\n",
      "{'DP': 32, 'VDB': 0.532986, 'SGB': -0.69311, 'MQSB': 0.916809, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 17, 14], 'MQ': 34, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=14499, REF=C, ALT=[T])\n",
      "35\n",
      "[0, 0, 9, 25]\n",
      "{'DP': 35, 'VDB': 0.417885, 'SGB': -0.693132, 'MQSB': 0.99981, 'MQ0F': 0.0285714, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 9, 25], 'MQ': 34, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=14610, REF=G, ALT=[A, T, C])\n",
      "55\n",
      "[0, 0, 3, 42]\n",
      "{'DP': 55, 'VDB': 0.0078431, 'SGB': -0.693147, 'MQSB': 0.935894, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 3, 42], 'MQ': 37, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=14721, REF=TA, ALT=[TAA])\n",
      "13\n",
      "[0, 1, 1, 4]\n",
      "{'INDEL': True, 'IDV': 7, 'IMF': 0.538462, 'DP': 13, 'VDB': 0.893344, 'SGB': -0.590765, 'MQSB': 1.0, 'MQ0F': 0.0, 'AF1': 0.829413, 'AC1': 1.0, 'DP4': [0, 1, 1, 4], 'MQ': 31, 'FQ': -999.0, 'PV4': [1.0, 1.0, 0.211525, 1.0]}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=14764, REF=T, ALT=[G])\n",
      "8\n",
      "[0, 0, 5, 2]\n",
      "{'DP': 8, 'VDB': 0.653727, 'SGB': -0.636426, 'MQSB': 0.8, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 5, 2], 'MQ': 37, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=14874, REF=A, ALT=[G])\n",
      "17\n",
      "[0, 0, 9, 8]\n",
      "{'DP': 17, 'VDB': 0.404483, 'SGB': -0.690438, 'MQSB': 0.48511, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 9, 8], 'MQ': 37, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=15545, REF=CTTTTT, ALT=[CTTTTTT])\n",
      "16\n",
      "[0, 0, 14, 1]\n",
      "{'INDEL': True, 'IDV': 12, 'IMF': 0.75, 'DP': 16, 'VDB': 0.107561, 'SGB': -0.688148, 'MQSB': 1.0, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 14, 1], 'MQ': 32, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=15605, REF=A, ALT=[G])\n",
      "34\n",
      "[0, 0, 18, 14]\n",
      "{'DP': 34, 'VDB': 0.0946132, 'SGB': -0.69312, 'MQSB': 0.0959281, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 18, 14], 'MQ': 36, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=16415, REF=C, ALT=[T])\n",
      "13\n",
      "[0, 0, 3, 9]\n",
      "{'DP': 13, 'VDB': 0.0523689, 'SGB': -0.680642, 'MQSB': 0.432745, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 3, 9], 'MQ': 36, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=16469, REF=T, ALT=[C, G])\n",
      "26\n",
      "[0, 0, 12, 12]\n",
      "{'DP': 26, 'VDB': 0.991832, 'SGB': -0.692831, 'MQSB': 0.617753, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 12, 12], 'MQ': 33, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=16498, REF=A, ALT=[G, T])\n",
      "27\n",
      "[0, 0, 9, 16]\n",
      "{'DP': 27, 'VDB': 0.100705, 'SGB': -0.692914, 'MQSB': 0.594978, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 9, 16], 'MQ': 34, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=18386, REF=T, ALT=[G, C])\n",
      "11\n",
      "[0, 0, 4, 7]\n",
      "{'DP': 11, 'VDB': 0.406086, 'SGB': -0.676189, 'MQSB': 0.0401934, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 4, 7], 'MQ': 38, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=19764, REF=T, ALT=[C])\n",
      "19\n",
      "[0, 0, 5, 10]\n",
      "{'DP': 19, 'VDB': 0.873702, 'SGB': -0.688148, 'MQSB': 0.544711, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 5, 10], 'MQ': 39, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=20014, REF=C, ALT=[T, G])\n",
      "8\n",
      "[0, 0, 1, 5]\n",
      "{'DP': 8, 'VDB': 0.0846985, 'SGB': -0.616816, 'MQSB': 1.0, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 1, 5], 'MQ': 34, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=20156, REF=C, ALT=[T])\n",
      "12\n",
      "[0, 0, 9, 1]\n",
      "{'DP': 12, 'VDB': 0.00186926, 'SGB': -0.670168, 'MQSB': 1.0, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 9, 1], 'MQ': 32, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=20167, REF=A, ALT=[G])\n",
      "5\n",
      "[0, 0, 2, 0]\n",
      "{'DP': 5, 'VDB': 0.14, 'SGB': -0.453602, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 2, 0], 'MQ': 24, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=20188, REF=C, ALT=[T])\n",
      "3\n",
      "[0, 0, 0, 3]\n",
      "{'DP': 3, 'VDB': 0.21346, 'SGB': -0.511536, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 0, 3], 'MQ': 35, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=20251, REF=G, ALT=[A])\n",
      "9\n",
      "[0, 0, 2, 4]\n",
      "{'DP': 9, 'VDB': 0.936955, 'SGB': -0.616816, 'MQSB': 0.75, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 2, 4], 'MQ': 37, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=20350, REF=G, ALT=[A])\n",
      "23\n",
      "[0, 0, 15, 5]\n",
      "{'DP': 23, 'VDB': 0.51179, 'SGB': -0.692067, 'MQSB': 0.991465, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 15, 5], 'MQ': 40, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=20617, REF=C, ALT=[T])\n",
      "21\n",
      "[0, 0, 6, 14]\n",
      "{'DP': 21, 'VDB': 0.306029, 'SGB': -0.692067, 'MQSB': 0.513417, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 6, 14], 'MQ': 38, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=21462, REF=T, ALT=[C, G])\n",
      "28\n",
      "[0, 0, 15, 10]\n",
      "{'DP': 28, 'VDB': 0.595584, 'SGB': -0.692914, 'MQSB': 0.121705, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 15, 10], 'MQ': 26, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=21466, REF=A, ALT=[G])\n",
      "28\n",
      "[0, 0, 10, 10]\n",
      "{'DP': 28, 'VDB': 0.9559, 'SGB': -0.692067, 'MQSB': 0.115243, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 10, 10], 'MQ': 27, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=22605, REF=A, ALT=[G])\n",
      "26\n",
      "[0, 0, 8, 17]\n",
      "{'DP': 26, 'VDB': 0.110825, 'SGB': -0.692914, 'MQSB': 0.998305, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 8, 17], 'MQ': 38, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=23103, REF=C, ALT=[T, A])\n",
      "13\n",
      "[0, 0, 9, 3]\n",
      "{'DP': 13, 'VDB': 0.287329, 'SGB': -0.680642, 'MQSB': 0.485672, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 9, 3], 'MQ': 37, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=23138, REF=T, ALT=[G])\n",
      "18\n",
      "[0, 1, 5, 10]\n",
      "{'DP': 18, 'VDB': 0.452471, 'SGB': -0.688148, 'RPB': 1.0, 'MQB': 1.0, 'MQSB': 0.960687, 'BQB': 1.0, 'MQ0F': 0.0555556, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 1, 5, 10], 'MQ': 35, 'FQ': -999.0, 'PV4': [1.0, 1.0, 0.236657, 1.0]}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=26021, REF=G, ALT=[A, C])\n",
      "13\n",
      "[0, 0, 2, 10]\n",
      "{'DP': 13, 'VDB': 0.394296, 'SGB': -0.680642, 'MQSB': 0.7, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 2, 10], 'MQ': 42, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=26070, REF=C, ALT=[T])\n",
      "37\n",
      "[0, 2, 17, 16]\n",
      "{'DP': 37, 'VDB': 0.000444035, 'SGB': -0.693127, 'RPB': 0.681818, 'MQB': 0.575758, 'MQSB': 0.854356, 'BQB': 0.212121, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 2, 17, 16], 'MQ': 40, 'FQ': -999.0, 'PV4': [0.485714, 1.0, 0.274129, 0.329459]}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=26173, REF=C, ALT=[G])\n",
      "2\n",
      "[0, 0, 1, 1]\n",
      "{'DP': 2, 'VDB': 0.6, 'SGB': -0.453602, 'MQSB': 1.0, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 1, 1], 'MQ': 40, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=26232, REF=A, ALT=[G])\n",
      "8\n",
      "[0, 0, 1, 7]\n",
      "{'DP': 8, 'VDB': 0.237012, 'SGB': -0.651104, 'MQSB': 1.0, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 1, 7], 'MQ': 38, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=27805, REF=ATTTTT, ALT=[ATTTTTT])\n",
      "12\n",
      "[0, 0, 5, 0]\n",
      "{'INDEL': True, 'IDV': 7, 'IMF': 0.583333, 'DP': 12, 'VDB': 0.210092, 'SGB': -0.590765, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 5, 0], 'MQ': 35, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=27885, REF=T, ALT=[C])\n",
      "34\n",
      "[0, 0, 18, 16]\n",
      "{'DP': 34, 'VDB': 0.0305142, 'SGB': -0.693132, 'MQSB': 0.00648609, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 18, 16], 'MQ': 39, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=27990, REF=G, ALT=[A, C])\n",
      "16\n",
      "[0, 0, 7, 6]\n",
      "{'DP': 16, 'VDB': 0.595846, 'SGB': -0.683931, 'MQSB': 0.623736, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 7, 6], 'MQ': 39, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=28047, REF=G, ALT=[A, T])\n",
      "21\n",
      "[0, 0, 6, 13]\n",
      "{'DP': 21, 'VDB': 0.0585785, 'SGB': -0.69168, 'MQSB': 0.350949, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 6, 13], 'MQ': 37, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=28175, REF=A, ALT=[G])\n",
      "24\n",
      "[0, 0, 11, 8]\n",
      "{'DP': 24, 'VDB': 0.016364, 'SGB': -0.69168, 'MQSB': 0.946915, 'MQ0F': 0.0833333, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 11, 8], 'MQ': 36, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=28224, REF=A, ALT=[G])\n",
      "25\n",
      "[0, 0, 10, 14]\n",
      "{'DP': 25, 'VDB': 0.220289, 'SGB': -0.692831, 'MQSB': 0.573826, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 10, 14], 'MQ': 36, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=28257, REF=A, ALT=[G, T])\n",
      "34\n",
      "[0, 0, 4, 28]\n",
      "{'DP': 34, 'VDB': 6.81048e-05, 'SGB': -0.69312, 'MQSB': 0.306223, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 4, 28], 'MQ': 31, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=29443, REF=T, ALT=[C])\n",
      "20\n",
      "[0, 0, 2, 16]\n",
      "{'DP': 20, 'VDB': 0.00267966, 'SGB': -0.691153, 'MQSB': 0.4375, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 2, 16], 'MQ': 40, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=29681, REF=AT, ALT=[A])\n",
      "24\n",
      "[0, 0, 9, 6]\n",
      "{'INDEL': True, 'IDV': 18, 'IMF': 0.75, 'DP': 24, 'VDB': 0.0742274, 'SGB': -0.688148, 'MQSB': 0.840624, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 9, 6], 'MQ': 33, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=29908, REF=G, ALT=[A])\n",
      "44\n",
      "[0, 0, 16, 26]\n",
      "{'DP': 44, 'VDB': 3.07683e-10, 'SGB': -0.693146, 'MQSB': 0.931907, 'MQ0F': 0.0227273, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 16, 26], 'MQ': 38, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=30289, REF=C, ALT=[T, A])\n",
      "10\n",
      "[0, 0, 4, 4]\n",
      "{'DP': 10, 'VDB': 0.585032, 'SGB': -0.651104, 'MQSB': 0.992367, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 4, 4], 'MQ': 32, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=30434, REF=T, ALT=[G])\n",
      "21\n",
      "[0, 1, 7, 12]\n",
      "{'DP': 21, 'VDB': 0.00921026, 'SGB': -0.69168, 'RPB': 1.0, 'MQB': 1.0, 'MQSB': 0.992961, 'BQB': 1.0, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 1, 7, 12], 'MQ': 39, 'FQ': -999.0, 'PV4': [1.0, 1.0, 0.398609, 0.46388]}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=30523, REF=C, ALT=[T])\n",
      "17\n",
      "[0, 0, 7, 10]\n",
      "{'DP': 17, 'VDB': 0.00232799, 'SGB': -0.690438, 'MQSB': 0.92664, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 7, 10], 'MQ': 40, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=30581, REF=A, ALT=[G])\n",
      "12\n",
      "[0, 0, 10, 2]\n",
      "{'DP': 12, 'VDB': 0.00127688, 'SGB': -0.680642, 'MQSB': 0.8, 'MQ0F': 0.0833333, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 10, 2], 'MQ': 34, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=30961, REF=A, ALT=[G])\n",
      "17\n",
      "[0, 0, 6, 8]\n",
      "{'DP': 17, 'VDB': 0.0171822, 'SGB': -0.686358, 'MQSB': 0.509156, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 6, 8], 'MQ': 35, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=30994, REF=G, ALT=[A])\n",
      "13\n",
      "[0, 0, 12, 1]\n",
      "{'DP': 13, 'VDB': 0.610232, 'SGB': -0.683931, 'MQSB': 1.0, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 12, 1], 'MQ': 37, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=31141, REF=A, ALT=[G])\n",
      "25\n",
      "[0, 0, 15, 9]\n",
      "{'DP': 25, 'VDB': 0.566267, 'SGB': -0.692831, 'MQSB': 0.978458, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 15, 9], 'MQ': 40, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=31633, REF=C, ALT=[A])\n",
      "12\n",
      "[0, 0, 2, 3]\n",
      "{'DP': 12, 'VDB': 0.422633, 'SGB': -0.590765, 'MQSB': 0.0, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 2, 3], 'MQ': 31, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=31954, REF=G, ALT=[A])\n",
      "35\n",
      "[0, 0, 10, 7]\n",
      "{'DP': 35, 'VDB': 0.428746, 'SGB': -0.690438, 'MQSB': 0.99881, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 10, 7], 'MQ': 38, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=32002, REF=C, ALT=[T, G, A])\n",
      "18\n",
      "[0, 0, 6, 10]\n",
      "{'DP': 18, 'VDB': 0.0336189, 'SGB': -0.689466, 'MQSB': 0.490777, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 6, 10], 'MQ': 40, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=32044, REF=A, ALT=[T])\n",
      "14\n",
      "[1, 0, 6, 6]\n",
      "{'DP': 14, 'VDB': 0.391706, 'SGB': -0.680642, 'RPB': 1.0, 'MQB': 1.0, 'MQSB': 0.920266, 'BQB': 1.0, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [1, 0, 6, 6], 'MQ': 38, 'FQ': -999.0, 'PV4': [1.0, 1.0, 0.25131, 1.0]}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=32266, REF=C, ALT=[T])\n",
      "6\n",
      "[0, 0, 3, 2]\n",
      "{'DP': 6, 'VDB': 0.0550147, 'SGB': -0.590765, 'MQSB': 0.0, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 3, 2], 'MQ': 34, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=32281, REF=A, ALT=[G])\n",
      "4\n",
      "[0, 0, 0, 3]\n",
      "{'DP': 4, 'VDB': 0.0681597, 'SGB': -0.511536, 'MQ0F': 0.25, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 0, 3], 'MQ': 30, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=32284, REF=C, ALT=[T])\n",
      "4\n",
      "[0, 0, 0, 3]\n",
      "{'DP': 4, 'VDB': 0.0681597, 'SGB': -0.511536, 'MQ0F': 0.25, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 0, 3], 'MQ': 30, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=32497, REF=A, ALT=[G])\n",
      "33\n",
      "[0, 0, 16, 16]\n",
      "{'DP': 33, 'VDB': 0.00348412, 'SGB': -0.69312, 'MQSB': 0.215307, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 16, 16], 'MQ': 38, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=32701, REF=T, ALT=[C])\n",
      "16\n",
      "[0, 0, 13, 1]\n",
      "{'DP': 16, 'VDB': 0.0117032, 'SGB': -0.686358, 'MQSB': 1.0, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 13, 1], 'MQ': 32, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=32713, REF=C, ALT=[T])\n",
      "13\n",
      "[0, 0, 7, 5]\n",
      "{'DP': 13, 'VDB': 0.985402, 'SGB': -0.680642, 'MQSB': 0.838008, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 7, 5], 'MQ': 33, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=32743, REF=G, ALT=[A])\n",
      "36\n",
      "[0, 0, 14, 20]\n",
      "{'DP': 36, 'VDB': 0.04022, 'SGB': -0.693132, 'MQSB': 0.966147, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 14, 20], 'MQ': 39, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=37272, REF=A, ALT=[G])\n",
      "25\n",
      "[0, 0, 3, 22]\n",
      "{'DP': 25, 'VDB': 1.67778e-06, 'SGB': -0.692914, 'MQSB': 0.604414, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 3, 22], 'MQ': 41, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=37294, REF=G, ALT=[A, C])\n",
      "6\n",
      "[0, 0, 2, 3]\n",
      "{'DP': 6, 'VDB': 0.233642, 'SGB': -0.590765, 'MQSB': 0.0, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 2, 3], 'MQ': 39, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=37402, REF=T, ALT=[C, G])\n",
      "11\n",
      "[0, 0, 4, 3]\n",
      "{'DP': 11, 'VDB': 0.405339, 'SGB': -0.636426, 'MQSB': 0.405132, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 4, 3], 'MQ': 29, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=37404, REF=C, ALT=[T, G, A])\n",
      "10\n",
      "[0, 0, 4, 3]\n",
      "{'DP': 10, 'VDB': 0.436369, 'SGB': -0.636426, 'MQSB': 0.405132, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 4, 3], 'MQ': 29, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=37564, REF=A, ALT=[G])\n",
      "30\n",
      "[0, 0, 9, 14]\n",
      "{'DP': 30, 'VDB': 0.115894, 'SGB': -0.692717, 'MQSB': 0.289361, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 9, 14], 'MQ': 38, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=37693, REF=A, ALT=[G, C])\n",
      "28\n",
      "[0, 0, 14, 12]\n",
      "{'DP': 28, 'VDB': 0.675262, 'SGB': -0.692976, 'MQSB': 0.216731, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 14, 12], 'MQ': 39, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=43220, REF=C, ALT=[T])\n",
      "30\n",
      "[0, 0, 21, 7]\n",
      "{'DP': 30, 'VDB': 0.742067, 'SGB': -0.693054, 'MQSB': 0.91386, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 21, 7], 'MQ': 35, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=43293, REF=A, ALT=[G])\n",
      "27\n",
      "[0, 0, 17, 9]\n",
      "{'DP': 27, 'VDB': 0.773287, 'SGB': -0.692976, 'MQSB': 0.418189, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 17, 9], 'MQ': 35, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=43328, REF=G, ALT=[A])\n",
      "38\n",
      "[0, 0, 28, 7]\n",
      "{'DP': 38, 'VDB': 0.321014, 'SGB': -0.693136, 'MQSB': 0.57526, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 28, 7], 'MQ': 39, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=43439, REF=C, ALT=[T])\n",
      "18\n",
      "[0, 0, 5, 13]\n",
      "{'DP': 18, 'VDB': 0.0069682, 'SGB': -0.691153, 'MQSB': 0.863325, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 5, 13], 'MQ': 40, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=43742, REF=T, ALT=[C])\n",
      "34\n",
      "[0, 0, 7, 23]\n",
      "{'DP': 34, 'VDB': 0.228077, 'SGB': -0.693097, 'MQSB': 0.0438567, 'MQ0F': 0.0294118, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 7, 23], 'MQ': 36, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=43850, REF=T, ALT=[G])\n",
      "29\n",
      "[0, 0, 20, 6]\n",
      "{'DP': 29, 'VDB': 0.172245, 'SGB': -0.692976, 'MQSB': 1.0, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 20, 6], 'MQ': 38, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=43895, REF=C, ALT=[T])\n",
      "45\n",
      "[0, 0, 13, 31]\n",
      "{'DP': 45, 'VDB': 0.00132604, 'SGB': -0.693146, 'MQSB': 0.937211, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 13, 31], 'MQ': 39, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=44501, REF=A, ALT=[G])\n",
      "23\n",
      "[0, 0, 10, 12]\n",
      "{'DP': 23, 'VDB': 0.00570971, 'SGB': -0.692562, 'MQSB': 0.854648, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 10, 12], 'MQ': 35, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=44699, REF=C, ALT=[A])\n",
      "15\n",
      "[0, 0, 6, 8]\n",
      "{'DP': 15, 'VDB': 0.241919, 'SGB': -0.686358, 'MQSB': 0.740818, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 6, 8], 'MQ': 38, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=44728, REF=G, ALT=[A, C])\n",
      "25\n",
      "[0, 0, 2, 20]\n",
      "{'DP': 25, 'VDB': 0.0113889, 'SGB': -0.692562, 'MQSB': 0.2, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 2, 20], 'MQ': 41, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=44877, REF=A, ALT=[G])\n",
      "14\n",
      "[0, 0, 13, 1]\n",
      "{'DP': 14, 'VDB': 0.139788, 'SGB': -0.686358, 'MQSB': 1.0, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 13, 1], 'MQ': 39, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=45569, REF=A, ALT=[G, T, C])\n",
      "19\n",
      "[0, 0, 8, 11]\n",
      "{'DP': 19, 'VDB': 0.000725691, 'SGB': -0.69168, 'MQSB': 0.587035, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 8, 11], 'MQ': 35, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=45884, REF=A, ALT=[G])\n",
      "26\n",
      "[0, 0, 15, 10]\n",
      "{'DP': 26, 'VDB': 0.308196, 'SGB': -0.692914, 'MQSB': 0.882836, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 15, 10], 'MQ': 41, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=46508, REF=A, ALT=[G, C])\n",
      "52\n",
      "[0, 0, 9, 39]\n",
      "{'DP': 52, 'VDB': 0.0417261, 'SGB': -0.693147, 'MQSB': 0.914564, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 9, 39], 'MQ': 38, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=46634, REF=C, ALT=[T])\n",
      "37\n",
      "[0, 0, 17, 18]\n",
      "{'DP': 37, 'VDB': 0.0775508, 'SGB': -0.693136, 'MQSB': 0.0932427, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 17, 18], 'MQ': 38, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=46717, REF=A, ALT=[G, C])\n",
      "17\n",
      "[0, 0, 12, 5]\n",
      "{'DP': 17, 'VDB': 0.906776, 'SGB': -0.690438, 'MQSB': 0.761685, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 12, 5], 'MQ': 36, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=46819, REF=C, ALT=[T])\n",
      "25\n",
      "[0, 0, 15, 10]\n",
      "{'DP': 25, 'VDB': 0.220231, 'SGB': -0.692914, 'MQSB': 0.927387, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 15, 10], 'MQ': 39, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=46852, REF=T, ALT=[C])\n",
      "12\n",
      "[0, 0, 4, 8]\n",
      "{'DP': 12, 'VDB': 0.848205, 'SGB': -0.680642, 'MQSB': 0.0249144, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 4, 8], 'MQ': 34, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=46906, REF=A, ALT=[T, G])\n",
      "20\n",
      "[2, 0, 8, 8]\n",
      "{'DP': 20, 'VDB': 0.891894, 'SGB': -0.689466, 'RPB': 0.75, 'MQB': 0.8125, 'MQSB': 0.906029, 'BQB': 0.3125, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [2, 0, 8, 8], 'MQ': 41, 'FQ': -999.0, 'PV4': [0.477124, 1.0, 0.339263, 0.117894]}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=47180, REF=G, ALT=[A])\n",
      "12\n",
      "[0, 0, 6, 4]\n",
      "{'DP': 12, 'VDB': 0.117011, 'SGB': -0.670168, 'MQSB': 0.167959, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 6, 4], 'MQ': 40, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=47777, REF=A, ALT=[G])\n",
      "32\n",
      "[0, 0, 19, 8]\n",
      "{'DP': 32, 'VDB': 0.00125761, 'SGB': -0.693021, 'MQSB': 0.843173, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 19, 8], 'MQ': 39, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=47813, REF=C, ALT=[T])\n",
      "24\n",
      "[0, 0, 8, 16]\n",
      "{'DP': 24, 'VDB': 0.0473017, 'SGB': -0.692831, 'MQSB': 0.0338195, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 8, 16], 'MQ': 38, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=48165, REF=A, ALT=[G])\n",
      "6\n",
      "[0, 0, 2, 4]\n",
      "{'DP': 6, 'VDB': 0.9708, 'SGB': -0.616816, 'MQSB': 0.25, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 2, 4], 'MQ': 29, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=48172, REF=G, ALT=[A])\n",
      "10\n",
      "[0, 0, 2, 3]\n",
      "{'DP': 10, 'VDB': 0.0236989, 'SGB': -0.590765, 'MQSB': 0.5, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 2, 3], 'MQ': 27, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=48513, REF=C, ALT=[T, A, G])\n",
      "45\n",
      "[0, 0, 31, 10]\n",
      "{'DP': 45, 'VDB': 0.0218846, 'SGB': -0.693145, 'MQSB': 0.0270699, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 31, 10], 'MQ': 28, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=48516, REF=T, ALT=[C, G])\n",
      "46\n",
      "[0, 0, 31, 10]\n",
      "{'DP': 46, 'VDB': 0.0225974, 'SGB': -0.693145, 'MQSB': 0.0270699, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 31, 10], 'MQ': 28, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=48564, REF=A, ALT=[G])\n",
      "37\n",
      "[0, 0, 15, 19]\n",
      "{'DP': 37, 'VDB': 0.402193, 'SGB': -0.693132, 'MQSB': 0.994601, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 15, 19], 'MQ': 36, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=48618, REF=A, ALT=[G])\n",
      "55\n",
      "[0, 0, 27, 28]\n",
      "{'DP': 55, 'VDB': 0.000176701, 'SGB': -0.693147, 'MQSB': 0.200396, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 27, 28], 'MQ': 39, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=48744, REF=G, ALT=[T])\n",
      "29\n",
      "[4, 0, 1, 24]\n",
      "{'DP': 29, 'VDB': 0.871526, 'SGB': -0.692914, 'RPB': 0.951229, 'MQB': 0.580132, 'MQSB': 0.398353, 'BQB': 0.0441351, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [4, 0, 1, 24], 'MQ': 38, 'FQ': -999.0, 'PV4': [0.000210517, 1.0, 0.133043, 0.0471668]}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=49832, REF=G, ALT=[T])\n",
      "25\n",
      "[3, 0, 14, 8]\n",
      "{'DP': 25, 'VDB': 0.0181563, 'SGB': -0.692562, 'RPB': 0.704935, 'MQB': 0.916299, 'MQSB': 0.0708293, 'BQB': 0.0429872, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [3, 0, 14, 8], 'MQ': 38, 'FQ': -999.0, 'PV4': [0.526956, 1.0, 1.0, 1.0]}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=49846, REF=C, ALT=[T])\n",
      "17\n",
      "[0, 0, 6, 7]\n",
      "{'DP': 17, 'VDB': 0.865136, 'SGB': -0.683931, 'MQSB': 0.695312, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 6, 7], 'MQ': 32, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=49892, REF=T, ALT=[C])\n",
      "17\n",
      "[0, 0, 10, 6]\n",
      "{'DP': 17, 'VDB': 0.247698, 'SGB': -0.689466, 'MQSB': 0.290322, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 10, 6], 'MQ': 37, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=50029, REF=G, ALT=[A])\n",
      "12\n",
      "[0, 0, 4, 5]\n",
      "{'DP': 12, 'VDB': 0.946069, 'SGB': -0.662043, 'MQSB': 0.406082, 'MQ0F': 0.0833333, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 4, 5], 'MQ': 28, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=50037, REF=G, ALT=[A])\n",
      "6\n",
      "[0, 0, 3, 2]\n",
      "{'DP': 6, 'VDB': 0.170249, 'SGB': -0.590765, 'MQSB': 0.166667, 'MQ0F': 0.166667, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 3, 2], 'MQ': 18, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=50064, REF=T, ALT=[C, A])\n",
      "13\n",
      "[0, 0, 5, 6]\n",
      "{'DP': 13, 'VDB': 0.768075, 'SGB': -0.676189, 'MQSB': 0.950952, 'MQ0F': 0.0769231, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 5, 6], 'MQ': 37, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=50233, REF=T, ALT=[C, G])\n",
      "43\n",
      "[0, 0, 31, 6]\n",
      "{'DP': 43, 'VDB': 0.00483067, 'SGB': -0.693141, 'MQSB': 0.0634144, 'MQ0F': 0.0465116, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 31, 6], 'MQ': 40, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=50269, REF=T, ALT=[C])\n",
      "28\n",
      "[0, 0, 20, 5]\n",
      "{'DP': 28, 'VDB': 0.0215562, 'SGB': -0.692914, 'MQSB': 0.829508, 'MQ0F': 0.142857, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 20, 5], 'MQ': 22, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=50272, REF=A, ALT=[G])\n",
      "27\n",
      "[0, 0, 18, 3]\n",
      "{'DP': 27, 'VDB': 0.0244341, 'SGB': -0.692352, 'MQSB': 0.807846, 'MQ0F': 0.148148, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 18, 3], 'MQ': 21, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=50408, REF=G, ALT=[A, C])\n",
      "27\n",
      "[0, 0, 9, 17]\n",
      "{'DP': 27, 'VDB': 0.00374181, 'SGB': -0.692976, 'MQSB': 0.864812, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 9, 17], 'MQ': 39, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=52778, REF=C, ALT=[T])\n",
      "7\n",
      "[0, 0, 4, 3]\n",
      "{'DP': 7, 'VDB': 0.00467508, 'SGB': -0.636426, 'MQSB': 0.810265, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 4, 3], 'MQ': 25, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=52789, REF=C, ALT=[T])\n",
      "8\n",
      "[0, 0, 5, 1]\n",
      "{'DP': 8, 'VDB': 0.565215, 'SGB': -0.616816, 'MQSB': 1.0, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 5, 1], 'MQ': 30, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=53788, REF=T, ALT=[A])\n",
      "14\n",
      "[0, 2, 2, 6]\n",
      "{'DP': 14, 'VDB': 0.316575, 'SGB': -0.651104, 'RPB': 0.6875, 'MQB': 0.375, 'MQSB': 0.0, 'BQB': 0.3125, 'MQ0F': 0.0, 'AF1': 0.571816, 'AC1': 1.0, 'DP4': [0, 2, 2, 6], 'MQ': 26, 'FQ': -999.0, 'PV4': [1.0, 1.0, 0.115895, 1.0]}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=53792, REF=T, ALT=[C])\n",
      "12\n",
      "[0, 0, 2, 10]\n",
      "{'DP': 12, 'VDB': 0.355991, 'SGB': -0.680642, 'MQSB': 0.0, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 2, 10], 'MQ': 29, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=53840, REF=G, ALT=[C])\n",
      "19\n",
      "[1, 0, 7, 7]\n",
      "{'DP': 19, 'VDB': 0.262455, 'SGB': -0.686358, 'RPB': 1.0, 'MQB': 1.0, 'MQSB': 0.68614, 'BQB': 1.0, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [1, 0, 7, 7], 'MQ': 36, 'FQ': -999.0, 'PV4': [1.0, 1.0, 0.260603, 0.0816548]}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=53954, REF=C, ALT=[A, T])\n",
      "14\n",
      "[0, 1, 7, 4]\n",
      "{'DP': 14, 'VDB': 0.128058, 'SGB': -0.676189, 'RPB': 1.0, 'MQB': 1.0, 'MQSB': 0.40926, 'BQB': 1.0, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 1, 7, 4], 'MQ': 38, 'FQ': -999.0, 'PV4': [0.416667, 1.0, 0.276607, 0.456182]}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=54051, REF=G, ALT=[A])\n",
      "10\n",
      "[0, 0, 6, 3]\n",
      "{'DP': 10, 'VDB': 0.00123678, 'SGB': -0.662043, 'MQSB': 0.462292, 'MQ0F': 0.4, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 6, 3], 'MQ': 28, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=54105, REF=T, ALT=[G])\n",
      "13\n",
      "[0, 0, 12, 0]\n",
      "{'DP': 13, 'VDB': 0.0468491, 'SGB': -0.680642, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 12, 0], 'MQ': 40, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=54230, REF=C, ALT=[T])\n",
      "45\n",
      "[0, 0, 8, 35]\n",
      "{'DP': 45, 'VDB': 0.250593, 'SGB': -0.693146, 'MQSB': 0.987898, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 8, 35], 'MQ': 36, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=54300, REF=A, ALT=[G])\n",
      "47\n",
      "[0, 0, 22, 19]\n",
      "{'DP': 47, 'VDB': 0.191273, 'SGB': -0.693145, 'MQSB': 0.905951, 'MQ0F': 0.0212766, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 22, 19], 'MQ': 36, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=54369, REF=T, ALT=[C])\n",
      "13\n",
      "[0, 0, 8, 4]\n",
      "{'DP': 13, 'VDB': 0.223642, 'SGB': -0.680642, 'MQSB': 0.397295, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 8, 4], 'MQ': 36, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=54493, REF=T, ALT=[C])\n",
      "16\n",
      "[0, 0, 8, 8]\n",
      "{'DP': 16, 'VDB': 1.42188e-05, 'SGB': -0.689466, 'MQSB': 0.136585, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 8, 8], 'MQ': 35, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=54507, REF=A, ALT=[G])\n",
      "5\n",
      "[0, 0, 2, 2]\n",
      "{'DP': 5, 'VDB': 0.775568, 'SGB': -0.556411, 'MQSB': 0.0, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 2, 2], 'MQ': 18, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=54534, REF=A, ALT=[G, C])\n",
      "24\n",
      "[0, 0, 20, 2]\n",
      "{'DP': 24, 'VDB': 0.990321, 'SGB': -0.692562, 'MQSB': 0.775, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 20, 2], 'MQ': 34, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=54858, REF=T, ALT=[C])\n",
      "36\n",
      "[0, 0, 10, 24]\n",
      "{'DP': 36, 'VDB': 0.00085763, 'SGB': -0.693132, 'MQSB': 0.982301, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 10, 24], 'MQ': 36, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=55190, REF=C, ALT=[G])\n",
      "22\n",
      "[0, 1, 6, 13]\n",
      "{'DP': 22, 'VDB': 0.989613, 'SGB': -0.69168, 'RPB': 1.0, 'MQB': 1.0, 'MQSB': 0.687289, 'BQB': 1.0, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 1, 6, 13], 'MQ': 39, 'FQ': -999.0, 'PV4': [1.0, 1.0, 0.286335, 0.222837]}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=55382, REF=A, ALT=[G])\n",
      "38\n",
      "[0, 0, 17, 16]\n",
      "{'DP': 38, 'VDB': 0.0873195, 'SGB': -0.693127, 'MQSB': 0.0793147, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 17, 16], 'MQ': 35, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=55496, REF=T, ALT=[C])\n",
      "15\n",
      "[0, 0, 11, 2]\n",
      "{'DP': 15, 'VDB': 0.0273298, 'SGB': -0.683931, 'MQSB': 0.909091, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 11, 2], 'MQ': 40, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=56635, REF=GAAAA, ALT=[GAAAAA])\n",
      "14\n",
      "[0, 0, 2, 1]\n",
      "{'INDEL': True, 'IDV': 6, 'IMF': 0.428571, 'DP': 14, 'VDB': 0.045681, 'SGB': -0.511536, 'MQSB': 1.0, 'MQ0F': 0.285714, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 2, 1], 'MQ': 30, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=56653, REF=ATTTTTT, ALT=[ATTTTTTTT, ATTTTTTT])\n",
      "13\n",
      "[0, 0, 3, 4]\n",
      "{'INDEL': True, 'IDV': 12, 'IMF': 0.923077, 'DP': 13, 'VDB': 0.0827521, 'SGB': -0.636426, 'MQSB': 0.810265, 'MQ0F': 0.384615, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 3, 4], 'MQ': 27, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=56823, REF=A, ALT=[G, C])\n",
      "24\n",
      "[0, 0, 10, 14]\n",
      "{'DP': 24, 'VDB': 0.784293, 'SGB': -0.692831, 'MQSB': 0.979219, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 10, 14], 'MQ': 35, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=59987, REF=C, ALT=[T, G])\n",
      "27\n",
      "[0, 0, 26, 0]\n",
      "{'DP': 27, 'VDB': 3.94339e-05, 'SGB': -0.692976, 'MQ0F': 0.0740741, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 26, 0], 'MQ': 24, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=59993, REF=A, ALT=[G])\n",
      "22\n",
      "[0, 0, 19, 0]\n",
      "{'DP': 22, 'VDB': 2.87987e-06, 'SGB': -0.69168, 'MQ0F': 0.0909091, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 19, 0], 'MQ': 21, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=60005, REF=T, ALT=[C])\n",
      "9\n",
      "[0, 0, 6, 0]\n",
      "{'DP': 9, 'VDB': 0.000757574, 'SGB': -0.616816, 'MQ0F': 0.222222, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 6, 0], 'MQ': 19, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=60023, REF=C, ALT=[T])\n",
      "10\n",
      "[0, 0, 4, 5]\n",
      "{'DP': 10, 'VDB': 0.00270021, 'SGB': -0.662043, 'MQSB': 0.89338, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 4, 5], 'MQ': 35, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=61952, REF=C, ALT=[T, G])\n",
      "33\n",
      "[0, 0, 22, 6]\n",
      "{'DP': 33, 'VDB': 0.00516941, 'SGB': -0.693054, 'MQSB': 0.452261, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 22, 6], 'MQ': 24, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=61955, REF=A, ALT=[G])\n",
      "31\n",
      "[0, 0, 21, 6]\n",
      "{'DP': 31, 'VDB': 0.00499167, 'SGB': -0.693021, 'MQSB': 0.439056, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 21, 6], 'MQ': 24, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=62186, REF=G, ALT=[T])\n",
      "34\n",
      "[3, 0, 9, 12]\n",
      "{'DP': 34, 'VDB': 0.488549, 'SGB': -0.692352, 'RPB': 0.891154, 'MQB': 0.101605, 'MQSB': 0.0272215, 'BQB': 0.851333, 'MQ0F': 0.0588235, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [3, 0, 9, 12], 'MQ': 32, 'FQ': -999.0, 'PV4': [0.217391, 1.0, 0.0322694, 1.0]}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=62189, REF=T, ALT=[C])\n",
      "32\n",
      "[0, 0, 8, 12]\n",
      "{'DP': 32, 'VDB': 0.923811, 'SGB': -0.692067, 'MQSB': 0.114218, 'MQ0F': 0.0625, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 8, 12], 'MQ': 30, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=62363, REF=A, ALT=[G])\n",
      "16\n",
      "[0, 0, 4, 8]\n",
      "{'DP': 16, 'VDB': 0.100499, 'SGB': -0.680642, 'MQSB': 0.793923, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 4, 8], 'MQ': 38, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=62524, REF=G, ALT=[A])\n",
      "19\n",
      "[0, 0, 5, 10]\n",
      "{'DP': 19, 'VDB': 0.593461, 'SGB': -0.688148, 'MQSB': 0.76338, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 5, 10], 'MQ': 37, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=63057, REF=C, ALT=[T, A])\n",
      "14\n",
      "[0, 0, 9, 0]\n",
      "{'DP': 14, 'VDB': 0.159195, 'SGB': -0.662043, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 9, 0], 'MQ': 32, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=63065, REF=A, ALT=[G])\n",
      "7\n",
      "[0, 0, 7, 0]\n",
      "{'DP': 7, 'VDB': 0.834632, 'SGB': -0.636426, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 7, 0], 'MQ': 30, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=63278, REF=A, ALT=[G])\n",
      "46\n",
      "[0, 0, 17, 27]\n",
      "{'DP': 46, 'VDB': 0.123466, 'SGB': -0.693146, 'MQSB': 0.562572, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 17, 27], 'MQ': 39, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=63374, REF=T, ALT=[C, G])\n",
      "32\n",
      "[0, 0, 26, 3]\n",
      "{'DP': 32, 'VDB': 0.0643056, 'SGB': -0.693079, 'MQSB': 0.133954, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 26, 3], 'MQ': 38, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=63527, REF=C, ALT=[A])\n",
      "49\n",
      "[0, 0, 11, 36]\n",
      "{'DP': 49, 'VDB': 0.492876, 'SGB': -0.693147, 'MQSB': 0.864234, 'MQ0F': 0.0204082, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 11, 36], 'MQ': 38, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=63725, REF=T, ALT=[C])\n",
      "40\n",
      "[0, 0, 6, 33]\n",
      "{'DP': 40, 'VDB': 0.00349934, 'SGB': -0.693144, 'MQSB': 0.162198, 'MQ0F': 0.025, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 6, 33], 'MQ': 39, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=64063, REF=C, ALT=[T, G])\n",
      "15\n",
      "[0, 0, 5, 6]\n",
      "{'DP': 15, 'VDB': 0.22041, 'SGB': -0.676189, 'MQSB': 0.950952, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 5, 6], 'MQ': 39, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=64222, REF=C, ALT=[T, G])\n",
      "25\n",
      "[0, 1, 13, 7]\n",
      "{'DP': 25, 'VDB': 0.944417, 'SGB': -0.692067, 'RPB': 1.0, 'MQB': 1.0, 'MQSB': 0.663818, 'BQB': 1.0, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 1, 13, 7], 'MQ': 38, 'FQ': -999.0, 'PV4': [0.380952, 1.0, 0.293745, 1.0]}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=64357, REF=T, ALT=[A])\n",
      "33\n",
      "[0, 5, 13, 8]\n",
      "{'DP': 33, 'VDB': 0.0445311, 'SGB': -0.692352, 'RPB': 0.122456, 'MQB': 0.266401, 'MQSB': 0.827498, 'BQB': 0.660463, 'MQ0F': 0.0909091, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 5, 13, 8], 'MQ': 30, 'FQ': -999.0, 'PV4': [0.0391304, 1.0, 0.0795636, 1.0]}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=64368, REF=A, ALT=[C])\n",
      "32\n",
      "[1, 0, 5, 24]\n",
      "{'DP': 32, 'VDB': 0.0358277, 'SGB': -0.693079, 'RPB': 1.0, 'MQB': 1.0, 'MQSB': 0.16685, 'BQB': 1.0, 'MQ0F': 0.125, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [1, 0, 5, 24], 'MQ': 26, 'FQ': -999.0, 'PV4': [0.2, 1.0, 1.0, 1.0]}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=65003, REF=T, ALT=[C])\n",
      "60\n",
      "[0, 0, 44, 9]\n",
      "{'DP': 60, 'VDB': 0.0551472, 'SGB': -0.693147, 'MQSB': 0.588055, 'MQ0F': 0.0166667, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 44, 9], 'MQ': 35, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=65439, REF=C, ALT=[T])\n",
      "10\n",
      "[0, 0, 6, 3]\n",
      "{'DP': 10, 'VDB': 0.0379436, 'SGB': -0.662043, 'MQSB': 0.115573, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 6, 3], 'MQ': 37, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=66022, REF=A, ALT=[G])\n",
      "34\n",
      "[1, 0, 19, 12]\n",
      "{'DP': 34, 'VDB': 0.955958, 'SGB': -0.69311, 'RPB': 1.0, 'MQB': 1.0, 'MQSB': 0.80337, 'BQB': 1.0, 'MQ0F': 0.0588235, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [1, 0, 19, 12], 'MQ': 24, 'FQ': -999.0, 'PV4': [1.0, 1.0, 1.0, 1.0]}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=66028, REF=T, ALT=[G])\n",
      "31\n",
      "[0, 0, 7, 16]\n",
      "{'DP': 31, 'VDB': 0.00521689, 'SGB': -0.692717, 'MQSB': 0.143342, 'MQ0F': 0.0322581, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 7, 16], 'MQ': 26, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=66058, REF=C, ALT=[T, G])\n",
      "12\n",
      "[0, 0, 4, 8]\n",
      "{'DP': 12, 'VDB': 0.487101, 'SGB': -0.680642, 'MQSB': 0.968069, 'MQ0F': 0.166667, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 4, 8], 'MQ': 31, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=66106, REF=A, ALT=[G])\n",
      "40\n",
      "[0, 0, 18, 13]\n",
      "{'DP': 40, 'VDB': 8.37202e-13, 'SGB': -0.69311, 'MQSB': 0.00864477, 'MQ0F': 0.075, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 18, 13], 'MQ': 27, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=66111, REF=T, ALT=[G])\n",
      "26\n",
      "[0, 0, 15, 4]\n",
      "{'DP': 26, 'VDB': 0.027115, 'SGB': -0.69168, 'MQSB': 0.164474, 'MQ0F': 0.0769231, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 15, 4], 'MQ': 20, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=66170, REF=T, ALT=[C, G])\n",
      "38\n",
      "[0, 0, 31, 1]\n",
      "{'DP': 38, 'VDB': 0.153942, 'SGB': -0.69312, 'MQSB': 1.0, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 31, 1], 'MQ': 39, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=70066, REF=A, ALT=[C])\n",
      "27\n",
      "[0, 0, 17, 0]\n",
      "{'DP': 27, 'VDB': 0.0262438, 'SGB': -0.690438, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 17, 0], 'MQ': 41, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=71081, REF=A, ALT=[G, T])\n",
      "31\n",
      "[0, 0, 4, 24]\n",
      "{'DP': 31, 'VDB': 0.470459, 'SGB': -0.693054, 'MQSB': 0.925347, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 4, 24], 'MQ': 40, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=71789, REF=C, ALT=[T, G])\n",
      "16\n",
      "[1, 0, 13, 2]\n",
      "{'DP': 16, 'VDB': 0.00587084, 'SGB': -0.688148, 'RPB': 1.0, 'MQB': 1.0, 'MQSB': 0.785714, 'BQB': 1.0, 'MQ0F': 0.0625, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [1, 0, 13, 2], 'MQ': 34, 'FQ': -999.0, 'PV4': [1.0, 1.0, 1.0, 1.0]}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=71813, REF=G, ALT=[A])\n",
      "3\n",
      "[0, 0, 1, 2]\n",
      "{'DP': 3, 'VDB': 0.0312982, 'SGB': -0.511536, 'MQSB': 1.0, 'MQ0F': 0.333333, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 1, 2], 'MQ': 24, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=71846, REF=T, ALT=[C])\n",
      "35\n",
      "[0, 0, 14, 21]\n",
      "{'DP': 35, 'VDB': 0.00761487, 'SGB': -0.693136, 'MQSB': 0.350573, 'MQ0F': 0.0285714, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 14, 21], 'MQ': 39, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=73674, REF=T, ALT=[C])\n",
      "43\n",
      "[0, 0, 4, 30]\n",
      "{'DP': 43, 'VDB': 0.000479259, 'SGB': -0.693132, 'MQSB': 0.866878, 'MQ0F': 0.0465116, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 4, 30], 'MQ': 35, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=73890, REF=T, ALT=[C])\n",
      "22\n",
      "[0, 1, 6, 10]\n",
      "{'DP': 22, 'VDB': 0.726572, 'SGB': -0.689466, 'RPB': 1.0, 'MQB': 1.0, 'MQSB': 0.664254, 'BQB': 1.0, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 1, 6, 10], 'MQ': 41, 'FQ': -999.0, 'PV4': [1.0, 1.0, 0.353607, 1.0]}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=74019, REF=A, ALT=[G, C])\n",
      "28\n",
      "[0, 0, 9, 17]\n",
      "{'DP': 28, 'VDB': 0.00204368, 'SGB': -0.692976, 'MQSB': 0.752257, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 9, 17], 'MQ': 41, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=74192, REF=A, ALT=[G, T])\n",
      "8\n",
      "[0, 0, 4, 4]\n",
      "{'DP': 8, 'VDB': 0.346875, 'SGB': -0.651104, 'MQSB': 0.124046, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 4, 4], 'MQ': 35, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=74407, REF=A, ALT=[G, C])\n",
      "24\n",
      "[0, 0, 1, 22]\n",
      "{'DP': 24, 'VDB': 0.00553345, 'SGB': -0.692717, 'MQSB': 1.0, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 1, 22], 'MQ': 40, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=74432, REF=A, ALT=[G])\n",
      "8\n",
      "[0, 0, 1, 7]\n",
      "{'DP': 8, 'VDB': 0.360182, 'SGB': -0.651104, 'MQSB': 1.0, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 1, 7], 'MQ': 39, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=74456, REF=T, ALT=[C])\n",
      "4\n",
      "[0, 0, 4, 0]\n",
      "{'DP': 4, 'VDB': 0.100421, 'SGB': -0.556411, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 4, 0], 'MQ': 37, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=74483, REF=C, ALT=[T, G])\n",
      "21\n",
      "[0, 0, 7, 10]\n",
      "{'DP': 21, 'VDB': 0.00024151, 'SGB': -0.690438, 'MQSB': 0.970677, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 7, 10], 'MQ': 35, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=75090, REF=A, ALT=[T])\n",
      "20\n",
      "[1, 0, 10, 8]\n",
      "{'DP': 20, 'VDB': 0.559746, 'SGB': -0.691153, 'RPB': 1.0, 'MQB': 1.0, 'MQSB': 0.395294, 'BQB': 1.0, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [1, 0, 10, 8], 'MQ': 39, 'FQ': -999.0, 'PV4': [1.0, 1.0, 0.409269, 1.0]}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=75197, REF=A, ALT=[G])\n",
      "38\n",
      "[0, 0, 29, 9]\n",
      "{'DP': 38, 'VDB': 0.552517, 'SGB': -0.693143, 'MQSB': 0.203138, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 29, 9], 'MQ': 36, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=75702, REF=T, ALT=[A])\n",
      "19\n",
      "[0, 2, 5, 5]\n",
      "{'DP': 19, 'VDB': 0.803036, 'SGB': -0.670168, 'RPB': 0.85, 'MQB': 0.4, 'MQSB': 0.935451, 'BQB': 0.65, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 2, 5, 5], 'MQ': 40, 'FQ': -999.0, 'PV4': [0.469697, 1.0, 0.253822, 0.320419]}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=75800, REF=C, ALT=[T])\n",
      "16\n",
      "[0, 0, 5, 11]\n",
      "{'DP': 16, 'VDB': 0.0233712, 'SGB': -0.689466, 'MQSB': 0.594648, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 5, 11], 'MQ': 38, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=78359, REF=G, ALT=[T])\n",
      "15\n",
      "[0, 0, 4, 11]\n",
      "{'DP': 15, 'VDB': 3.68681e-07, 'SGB': -0.688148, 'MQSB': 0.264034, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 4, 11], 'MQ': 34, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=78370, REF=A, ALT=[G])\n",
      "5\n",
      "[0, 0, 3, 1]\n",
      "{'DP': 5, 'VDB': 0.840365, 'SGB': -0.556411, 'MQSB': 1.0, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 3, 1], 'MQ': 41, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=78622, REF=C, ALT=[T])\n",
      "38\n",
      "[0, 0, 22, 15]\n",
      "{'DP': 38, 'VDB': 0.0106374, 'SGB': -0.693141, 'MQSB': 0.274232, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 22, 15], 'MQ': 39, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=78859, REF=A, ALT=[G])\n",
      "7\n",
      "[0, 0, 0, 5]\n",
      "{'DP': 7, 'VDB': 0.206846, 'SGB': -0.590765, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 0, 5], 'MQ': 41, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=78883, REF=C, ALT=[T, A])\n",
      "20\n",
      "[0, 0, 13, 6]\n",
      "{'DP': 20, 'VDB': 0.0531549, 'SGB': -0.69168, 'MQSB': 0.870697, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 13, 6], 'MQ': 39, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=78925, REF=G, ALT=[A])\n",
      "28\n",
      "[0, 0, 14, 10]\n",
      "{'DP': 28, 'VDB': 0.233425, 'SGB': -0.692831, 'MQSB': 0.357364, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 14, 10], 'MQ': 38, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=79043, REF=A, ALT=[G])\n",
      "16\n",
      "[0, 0, 8, 7]\n",
      "{'DP': 16, 'VDB': 0.616449, 'SGB': -0.688148, 'MQSB': 0.941512, 'MQ0F': 0.25, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 8, 7], 'MQ': 21, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=79045, REF=C, ALT=[T])\n",
      "16\n",
      "[0, 0, 8, 6]\n",
      "{'DP': 16, 'VDB': 0.460669, 'SGB': -0.686358, 'MQSB': 0.997919, 'MQ0F': 0.25, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 8, 6], 'MQ': 19, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=79075, REF=A, ALT=[G, C])\n",
      "33\n",
      "[0, 0, 9, 22]\n",
      "{'DP': 33, 'VDB': 0.050883, 'SGB': -0.69311, 'MQSB': 0.140793, 'MQ0F': 0.0606061, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 9, 22], 'MQ': 36, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=79204, REF=A, ALT=[G])\n",
      "14\n",
      "[0, 0, 8, 3]\n",
      "{'DP': 14, 'VDB': 0.978689, 'SGB': -0.676189, 'MQSB': 0.716531, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 8, 3], 'MQ': 38, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=79579, REF=T, ALT=[C])\n",
      "39\n",
      "[0, 0, 17, 17]\n",
      "{'DP': 39, 'VDB': 0.879949, 'SGB': -0.693132, 'MQSB': 0.43424, 'MQ0F': 0.0512821, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 17, 17], 'MQ': 38, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=79636, REF=G, ALT=[A])\n",
      "18\n",
      "[1, 0, 8, 9]\n",
      "{'DP': 18, 'VDB': 0.0426778, 'SGB': -0.690438, 'RPB': 1.0, 'MQB': 1.0, 'MQSB': 0.77918, 'BQB': 1.0, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [1, 0, 8, 9], 'MQ': 31, 'FQ': -999.0, 'PV4': [1.0, 1.0, 0.0808294, 0.17021]}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=79641, REF=C, ALT=[T, G, A])\n",
      "21\n",
      "[0, 0, 16, 2]\n",
      "{'DP': 21, 'VDB': 0.993823, 'SGB': -0.691153, 'MQSB': 0.625, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 16, 2], 'MQ': 32, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=80339, REF=T, ALT=[A])\n",
      "51\n",
      "[0, 4, 18, 28]\n",
      "{'DP': 51, 'VDB': 0.00096907, 'SGB': -0.693147, 'RPB': 0.290007, 'MQB': 0.63826, 'MQSB': 0.612381, 'BQB': 0.812889, 'MQ0F': 0.0196078, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 4, 18, 28], 'MQ': 39, 'FQ': -999.0, 'PV4': [0.282814, 1.0, 0.205785, 1.0]}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=80384, REF=TAAA, ALT=[TAA])\n",
      "10\n",
      "[0, 0, 0, 2]\n",
      "{'INDEL': True, 'IDV': 4, 'IMF': 0.4, 'DP': 10, 'VDB': 0.6, 'SGB': -0.453602, 'MQ0F': 0.2, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 0, 2], 'MQ': 33, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=80443, REF=G, ALT=[A])\n",
      "7\n",
      "[0, 0, 4, 2]\n",
      "{'DP': 7, 'VDB': 0.00324086, 'SGB': -0.616816, 'MQSB': 0.0, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 4, 2], 'MQ': 30, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=80456, REF=T, ALT=[G])\n",
      "7\n",
      "[0, 0, 4, 1]\n",
      "{'DP': 7, 'VDB': 0.0104213, 'SGB': -0.590765, 'MQSB': 1.0, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 4, 1], 'MQ': 36, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=80513, REF=C, ALT=[T, G])\n",
      "21\n",
      "[0, 0, 18, 3]\n",
      "{'DP': 21, 'VDB': 0.0556231, 'SGB': -0.692352, 'MQSB': 0.664254, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 18, 3], 'MQ': 35, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=80544, REF=A, ALT=[G])\n",
      "15\n",
      "[0, 0, 9, 4]\n",
      "{'DP': 15, 'VDB': 0.251386, 'SGB': -0.683931, 'MQSB': 0.864302, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 9, 4], 'MQ': 26, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=80552, REF=G, ALT=[A])\n",
      "11\n",
      "[0, 0, 4, 4]\n",
      "{'DP': 11, 'VDB': 0.333755, 'SGB': -0.651104, 'MQSB': 0.62023, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 4, 4], 'MQ': 22, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=80606, REF=C, ALT=[T, A])\n",
      "21\n",
      "[0, 0, 16, 4]\n",
      "{'DP': 21, 'VDB': 0.00172453, 'SGB': -0.692067, 'MQSB': 0.982301, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 16, 4], 'MQ': 35, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=80759, REF=C, ALT=[T])\n",
      "34\n",
      "[0, 0, 12, 21]\n",
      "{'DP': 34, 'VDB': 0.0601082, 'SGB': -0.693127, 'MQSB': 0.242182, 'MQ0F': 0.0294118, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 12, 21], 'MQ': 40, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=80921, REF=A, ALT=[T])\n",
      "6\n",
      "[1, 0, 4, 0]\n",
      "{'DP': 6, 'VDB': 0.775568, 'SGB': -0.556411, 'RPB': 1.0, 'MQB': 1.0, 'BQB': 1.0, 'MQ0F': 0.0, 'AF1': 0.667176, 'AC1': 1.0, 'DP4': [1, 0, 4, 0], 'MQ': 41, 'FQ': -999.0, 'PV4': [1.0, 1.0, 0.247513, 1.0]}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=80945, REF=T, ALT=[C])\n",
      "18\n",
      "[0, 0, 8, 7]\n",
      "{'DP': 18, 'VDB': 0.02696, 'SGB': -0.688148, 'MQSB': 0.921243, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 8, 7], 'MQ': 38, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=81124, REF=A, ALT=[G])\n",
      "11\n",
      "[0, 0, 8, 0]\n",
      "{'DP': 11, 'VDB': 0.396463, 'SGB': -0.651104, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 8, 0], 'MQ': 35, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=81147, REF=TAAA, ALT=[TAAAA])\n",
      "25\n",
      "[0, 0, 4, 0]\n",
      "{'INDEL': True, 'IDV': 16, 'IMF': 0.64, 'DP': 25, 'VDB': 0.01126, 'SGB': -0.556411, 'MQ0F': 0.36, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 4, 0], 'MQ': 23, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=81154, REF=TAAAA, ALT=[TAAAAA])\n",
      "25\n",
      "[0, 0, 0, 3]\n",
      "{'INDEL': True, 'IDV': 16, 'IMF': 0.64, 'DP': 25, 'VDB': 0.0389723, 'SGB': -0.511536, 'MQ0F': 0.36, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 0, 3], 'MQ': 41, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=81379, REF=C, ALT=[A])\n",
      "42\n",
      "[0, 2, 20, 17]\n",
      "{'DP': 42, 'VDB': 0.702465, 'SGB': -0.693141, 'RPB': 0.540541, 'MQB': 0.459459, 'MQSB': 0.867188, 'BQB': 0.108108, 'MQ0F': 0.0238095, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 2, 20, 17], 'MQ': 39, 'FQ': -999.0, 'PV4': [0.230769, 1.0, 0.260803, 1.0]}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=81757, REF=T, ALT=[C, A])\n",
      "13\n",
      "[0, 0, 7, 3]\n",
      "{'DP': 13, 'VDB': 0.0436921, 'SGB': -0.670168, 'MQSB': 0.366593, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 7, 3], 'MQ': 36, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=81784, REF=G, ALT=[A])\n",
      "6\n",
      "[0, 0, 4, 1]\n",
      "{'DP': 6, 'VDB': 0.724679, 'SGB': -0.590765, 'MQSB': 1.0, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 4, 1], 'MQ': 38, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=81808, REF=A, ALT=[G])\n",
      "26\n",
      "[0, 0, 7, 18]\n",
      "{'DP': 26, 'VDB': 0.0112067, 'SGB': -0.692914, 'MQSB': 0.889393, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 7, 18], 'MQ': 34, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=81859, REF=C, ALT=[A])\n",
      "15\n",
      "[0, 0, 6, 8]\n",
      "{'DP': 15, 'VDB': 0.76967, 'SGB': -0.686358, 'MQSB': 0.94925, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 6, 8], 'MQ': 37, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=82123, REF=C, ALT=[T])\n",
      "17\n",
      "[0, 0, 5, 8]\n",
      "{'DP': 17, 'VDB': 0.553116, 'SGB': -0.683931, 'MQSB': 0.958048, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 5, 8], 'MQ': 39, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=82177, REF=T, ALT=[C])\n",
      "34\n",
      "[0, 0, 18, 14]\n",
      "{'DP': 34, 'VDB': 0.987445, 'SGB': -0.69312, 'MQSB': 0.0225417, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 18, 14], 'MQ': 38, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=82419, REF=G, ALT=[T])\n",
      "23\n",
      "[0, 0, 14, 8]\n",
      "{'DP': 23, 'VDB': 0.050026, 'SGB': -0.692562, 'MQSB': 0.247066, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 14, 8], 'MQ': 39, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=82510, REF=A, ALT=[G, C])\n",
      "30\n",
      "[0, 0, 11, 17]\n",
      "{'DP': 30, 'VDB': 0.347253, 'SGB': -0.693054, 'MQSB': 0.333596, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 11, 17], 'MQ': 42, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=82666, REF=C, ALT=[T])\n",
      "17\n",
      "[0, 1, 9, 5]\n",
      "{'DP': 17, 'VDB': 0.677448, 'SGB': -0.686358, 'RPB': 1.0, 'MQB': 1.0, 'MQSB': 0.972604, 'BQB': 1.0, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 1, 9, 5], 'MQ': 36, 'FQ': -999.0, 'PV4': [0.4, 0.253291, 0.234214, 1.0]}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=82711, REF=A, ALT=[G])\n",
      "18\n",
      "[0, 0, 4, 9]\n",
      "{'DP': 18, 'VDB': 0.474283, 'SGB': -0.683931, 'MQSB': 0.742584, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 4, 9], 'MQ': 39, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=82930, REF=T, ALT=[C])\n",
      "19\n",
      "[0, 0, 15, 3]\n",
      "{'DP': 19, 'VDB': 0.293208, 'SGB': -0.691153, 'MQSB': 1.0, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 15, 3], 'MQ': 39, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=82966, REF=T, ALT=[A])\n",
      "22\n",
      "[0, 0, 17, 4]\n",
      "{'DP': 22, 'VDB': 0.000891448, 'SGB': -0.692352, 'MQSB': 0.984085, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 17, 4], 'MQ': 34, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=82999, REF=A, ALT=[G])\n",
      "5\n",
      "[0, 0, 1, 4]\n",
      "{'DP': 5, 'VDB': 0.922588, 'SGB': -0.590765, 'MQSB': 1.0, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 1, 4], 'MQ': 35, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=83013, REF=T, ALT=[C])\n",
      "18\n",
      "[0, 0, 3, 14]\n",
      "{'DP': 18, 'VDB': 1.59896e-06, 'SGB': -0.690438, 'MQSB': 0.350077, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 3, 14], 'MQ': 37, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=83062, REF=C, ALT=[T])\n",
      "6\n",
      "[0, 0, 1, 5]\n",
      "{'DP': 6, 'VDB': 0.381141, 'SGB': -0.616816, 'MQSB': 1.0, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 1, 5], 'MQ': 36, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=83207, REF=A, ALT=[G])\n",
      "12\n",
      "[0, 0, 9, 3]\n",
      "{'DP': 12, 'VDB': 0.176605, 'SGB': -0.680642, 'MQSB': 0.432745, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 9, 3], 'MQ': 34, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=83271, REF=T, ALT=[C])\n",
      "24\n",
      "[0, 0, 11, 13]\n",
      "{'DP': 24, 'VDB': 0.997328, 'SGB': -0.692831, 'MQSB': 0.999581, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 11, 13], 'MQ': 36, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=83598, REF=T, ALT=[C])\n",
      "17\n",
      "[0, 0, 5, 7]\n",
      "{'DP': 17, 'VDB': 0.00136466, 'SGB': -0.680642, 'MQSB': 0.6821, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 5, 7], 'MQ': 37, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=83676, REF=A, ALT=[G, T])\n",
      "24\n",
      "[0, 0, 6, 15]\n",
      "{'DP': 24, 'VDB': 0.231566, 'SGB': -0.692352, 'MQSB': 0.354474, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 6, 15], 'MQ': 35, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=85467, REF=C, ALT=[T])\n",
      "23\n",
      "[0, 0, 12, 5]\n",
      "{'DP': 23, 'VDB': 0.417749, 'SGB': -0.690438, 'MQSB': 0.761685, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 12, 5], 'MQ': 40, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=85811, REF=G, ALT=[A])\n",
      "32\n",
      "[0, 0, 24, 8]\n",
      "{'DP': 32, 'VDB': 0.107778, 'SGB': -0.69312, 'MQSB': 0.735784, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 24, 8], 'MQ': 38, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=85871, REF=T, ALT=[C])\n",
      "25\n",
      "[0, 0, 7, 13]\n",
      "{'DP': 25, 'VDB': 0.322032, 'SGB': -0.692067, 'MQSB': 0.962269, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 7, 13], 'MQ': 39, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=86054, REF=A, ALT=[C])\n",
      "37\n",
      "[2, 0, 26, 3]\n",
      "{'DP': 37, 'VDB': 0.0276365, 'SGB': -0.693079, 'RPB': 0.448276, 'MQB': 0.827586, 'MQSB': 0.705554, 'BQB': 0.517241, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [2, 0, 26, 3], 'MQ': 39, 'FQ': -999.0, 'PV4': [1.0, 1.0, 1.0, 0.0373937]}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=86151, REF=T, ALT=[C, A, G])\n",
      "14\n",
      "[0, 0, 10, 4]\n",
      "{'DP': 14, 'VDB': 0.0810482, 'SGB': -0.686358, 'MQSB': 0.960789, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 10, 4], 'MQ': 39, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=86224, REF=G, ALT=[A])\n",
      "22\n",
      "[0, 0, 6, 15]\n",
      "{'DP': 22, 'VDB': 0.999761, 'SGB': -0.692352, 'MQSB': 1.0, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 6, 15], 'MQ': 36, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=86449, REF=T, ALT=[C])\n",
      "34\n",
      "[0, 0, 30, 4]\n",
      "{'DP': 34, 'VDB': 0.106595, 'SGB': -0.693132, 'MQSB': 0.613283, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 30, 4], 'MQ': 36, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=90932, REF=T, ALT=[G, C])\n",
      "13\n",
      "[0, 1, 5, 7]\n",
      "{'DP': 13, 'VDB': 0.394296, 'SGB': -0.680642, 'RPB': 1.0, 'MQB': 1.0, 'MQSB': 0.84246, 'BQB': 1.0, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 1, 5, 7], 'MQ': 39, 'FQ': -999.0, 'PV4': [1.0, 1.0, 0.306303, 0.495354]}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=90980, REF=C, ALT=[T, A])\n",
      "2\n",
      "[0, 0, 1, 1]\n",
      "{'DP': 2, 'VDB': 0.28, 'SGB': -0.453602, 'MQSB': 1.0, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 1, 1], 'MQ': 41, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=90992, REF=G, ALT=[A])\n",
      "8\n",
      "[0, 0, 8, 0]\n",
      "{'DP': 8, 'VDB': 0.206858, 'SGB': -0.651104, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 8, 0], 'MQ': 40, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=91076, REF=A, ALT=[G])\n",
      "30\n",
      "[0, 0, 16, 12]\n",
      "{'DP': 30, 'VDB': 3.85723e-05, 'SGB': -0.693054, 'MQSB': 0.897844, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 16, 12], 'MQ': 38, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=91132, REF=C, ALT=[A])\n",
      "18\n",
      "[0, 0, 11, 4]\n",
      "{'DP': 18, 'VDB': 0.115729, 'SGB': -0.688148, 'MQSB': 0.872525, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 11, 4], 'MQ': 39, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=91172, REF=C, ALT=[T])\n",
      "38\n",
      "[0, 1, 23, 13]\n",
      "{'DP': 38, 'VDB': 0.0499416, 'SGB': -0.693139, 'RPB': 1.0, 'MQB': 1.0, 'MQSB': 0.0611055, 'BQB': 1.0, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 1, 23, 13], 'MQ': 39, 'FQ': -999.0, 'PV4': [0.378378, 1.0, 0.286271, 1.0]}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=91202, REF=A, ALT=[G])\n",
      "34\n",
      "[0, 0, 9, 23]\n",
      "{'DP': 34, 'VDB': 0.533778, 'SGB': -0.69312, 'MQSB': 0.96357, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 9, 23], 'MQ': 36, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=91251, REF=G, ALT=[A, C, T])\n",
      "30\n",
      "[0, 0, 13, 14]\n",
      "{'DP': 30, 'VDB': 0.410186, 'SGB': -0.693021, 'MQSB': 0.397295, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 13, 14], 'MQ': 37, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=91372, REF=T, ALT=[C])\n",
      "26\n",
      "[0, 0, 12, 9]\n",
      "{'DP': 26, 'VDB': 0.281035, 'SGB': -0.692352, 'MQSB': 0.796201, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 12, 9], 'MQ': 40, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=91535, REF=A, ALT=[G])\n",
      "12\n",
      "[0, 0, 7, 4]\n",
      "{'DP': 12, 'VDB': 0.212183, 'SGB': -0.676189, 'MQSB': 0.803868, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 7, 4], 'MQ': 41, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=91594, REF=G, ALT=[A])\n",
      "33\n",
      "[0, 0, 14, 16]\n",
      "{'DP': 33, 'VDB': 0.814533, 'SGB': -0.693097, 'MQSB': 0.532647, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 14, 16], 'MQ': 40, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=91832, REF=C, ALT=[T, G])\n",
      "17\n",
      "[0, 0, 10, 6]\n",
      "{'DP': 17, 'VDB': 0.992022, 'SGB': -0.689466, 'MQSB': 0.620971, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 10, 6], 'MQ': 40, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=94994, REF=C, ALT=[T])\n",
      "24\n",
      "[0, 0, 6, 16]\n",
      "{'DP': 24, 'VDB': 0.0761553, 'SGB': -0.692562, 'MQSB': 0.676174, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 6, 16], 'MQ': 38, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=95024, REF=C, ALT=[T])\n",
      "16\n",
      "[0, 0, 2, 12]\n",
      "{'DP': 16, 'VDB': 0.1448, 'SGB': -0.686358, 'MQSB': 0.583333, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 2, 12], 'MQ': 36, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=95666, REF=A, ALT=[G])\n",
      "84\n",
      "[0, 0, 14, 67]\n",
      "{'DP': 84, 'VDB': 7.2105e-09, 'SGB': -0.693147, 'MQSB': 0.708244, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 14, 67], 'MQ': 38, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=95852, REF=A, ALT=[G])\n",
      "32\n",
      "[0, 0, 9, 18]\n",
      "{'DP': 32, 'VDB': 0.561844, 'SGB': -0.693021, 'MQSB': 0.983927, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 9, 18], 'MQ': 39, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=97891, REF=G, ALT=[A])\n",
      "22\n",
      "[0, 0, 7, 12]\n",
      "{'DP': 22, 'VDB': 0.0496011, 'SGB': -0.69168, 'MQSB': 0.314383, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 7, 12], 'MQ': 39, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=97944, REF=G, ALT=[A])\n",
      "11\n",
      "[0, 0, 8, 2]\n",
      "{'DP': 11, 'VDB': 0.138695, 'SGB': -0.670168, 'MQSB': 0.4375, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 8, 2], 'MQ': 37, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=98094, REF=A, ALT=[G])\n",
      "26\n",
      "[0, 0, 10, 11]\n",
      "{'DP': 26, 'VDB': 0.933366, 'SGB': -0.692352, 'MQSB': 0.115597, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 10, 11], 'MQ': 38, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=98133, REF=A, ALT=[G])\n",
      "19\n",
      "[0, 0, 9, 10]\n",
      "{'DP': 19, 'VDB': 0.00080603, 'SGB': -0.69168, 'MQSB': 1.0, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 9, 10], 'MQ': 40, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=98666, REF=C, ALT=[T])\n",
      "8\n",
      "[0, 0, 7, 1]\n",
      "{'DP': 8, 'VDB': 0.052315, 'SGB': -0.651104, 'MQSB': 1.0, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 7, 1], 'MQ': 26, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=98672, REF=G, ALT=[T])\n",
      "8\n",
      "[1, 0, 4, 1]\n",
      "{'DP': 8, 'VDB': 0.0765947, 'SGB': -0.590765, 'RPB': 1.0, 'MQB': 1.0, 'MQSB': 1.0, 'BQB': 1.0, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [1, 0, 4, 1], 'MQ': 22, 'FQ': -999.0, 'PV4': [1.0, 1.0, 0.0236699, 1.0]}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=98691, REF=G, ALT=[A, T])\n",
      "9\n",
      "[0, 0, 3, 6]\n",
      "{'DP': 9, 'VDB': 0.00984062, 'SGB': -0.662043, 'MQSB': 0.577865, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 3, 6], 'MQ': 34, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=98717, REF=T, ALT=[G])\n",
      "34\n",
      "[0, 2, 5, 20]\n",
      "{'DP': 34, 'VDB': 0.0656639, 'SGB': -0.692914, 'RPB': 0.84, 'MQB': 0.82, 'MQSB': 0.942774, 'BQB': 0.7, 'MQ0F': 0.0294118, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 2, 5, 20], 'MQ': 39, 'FQ': -999.0, 'PV4': [1.0, 1.0, 0.297313, 1.0]}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=98782, REF=T, ALT=[G])\n",
      "21\n",
      "[0, 1, 4, 10]\n",
      "{'DP': 21, 'VDB': 0.580506, 'SGB': -0.686358, 'RPB': 1.0, 'MQB': 1.0, 'MQSB': 0.579578, 'BQB': 1.0, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 1, 4, 10], 'MQ': 37, 'FQ': -999.0, 'PV4': [1.0, 1.0, 0.266403, 0.148915]}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=98865, REF=G, ALT=[A, T])\n",
      "33\n",
      "[0, 0, 10, 21]\n",
      "{'DP': 33, 'VDB': 0.00362312, 'SGB': -0.69311, 'MQSB': 0.699673, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 10, 21], 'MQ': 37, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=99301, REF=A, ALT=[G])\n",
      "22\n",
      "[0, 0, 14, 7]\n",
      "{'DP': 22, 'VDB': 0.0602601, 'SGB': -0.692352, 'MQSB': 0.817858, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 14, 7], 'MQ': 38, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=99367, REF=A, ALT=[G])\n",
      "41\n",
      "[0, 0, 19, 15]\n",
      "{'DP': 41, 'VDB': 0.00273809, 'SGB': -0.693132, 'MQSB': 0.0787597, 'MQ0F': 0.0243902, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 19, 15], 'MQ': 38, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=99445, REF=T, ALT=[C])\n",
      "14\n",
      "[0, 0, 10, 3]\n",
      "{'DP': 14, 'VDB': 0.814382, 'SGB': -0.683931, 'MQSB': 0.914584, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 10, 3], 'MQ': 34, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=99499, REF=T, ALT=[C])\n",
      "38\n",
      "[0, 0, 5, 26]\n",
      "{'DP': 38, 'VDB': 0.0301662, 'SGB': -0.69311, 'MQSB': 0.922074, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 5, 26], 'MQ': 37, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=100627, REF=T, ALT=[C, G])\n",
      "27\n",
      "[0, 0, 15, 6]\n",
      "{'DP': 27, 'VDB': 0.000181255, 'SGB': -0.692352, 'MQSB': 0.354474, 'MQ0F': 0.0740741, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 15, 6], 'MQ': 24, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=100629, REF=C, ALT=[A])\n",
      "25\n",
      "[0, 0, 11, 6]\n",
      "{'DP': 25, 'VDB': 0.0029828, 'SGB': -0.690438, 'MQSB': 0.345809, 'MQ0F': 0.08, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 11, 6], 'MQ': 25, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=100642, REF=C, ALT=[A])\n",
      "15\n",
      "[0, 1, 5, 8]\n",
      "{'DP': 15, 'VDB': 0.832957, 'SGB': -0.683931, 'RPB': 1.0, 'MQB': 1.0, 'MQSB': 0.726149, 'BQB': 1.0, 'MQ0F': 0.133333, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 1, 5, 8], 'MQ': 28, 'FQ': -999.0, 'PV4': [1.0, 1.0, 0.469795, 0.0411523]}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=100681, REF=G, ALT=[T])\n",
      "18\n",
      "[0, 0, 2, 16]\n",
      "{'DP': 18, 'VDB': 0.000145084, 'SGB': -0.691153, 'MQSB': 0.5, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 2, 16], 'MQ': 39, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=100795, REF=A, ALT=[G])\n",
      "38\n",
      "[0, 0, 9, 26]\n",
      "{'DP': 38, 'VDB': 0.26105, 'SGB': -0.693136, 'MQSB': 0.937742, 'MQ0F': 0.0263158, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 9, 26], 'MQ': 39, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=100921, REF=A, ALT=[G])\n",
      "11\n",
      "[0, 0, 4, 7]\n",
      "{'DP': 11, 'VDB': 0.786011, 'SGB': -0.676189, 'MQSB': 0.924449, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 4, 7], 'MQ': 36, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=100993, REF=G, ALT=[A])\n",
      "24\n",
      "[0, 0, 4, 15]\n",
      "{'DP': 24, 'VDB': 0.858167, 'SGB': -0.69168, 'MQSB': 0.882497, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 4, 15], 'MQ': 37, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=103025, REF=T, ALT=[C, G, A])\n",
      "28\n",
      "[0, 0, 22, 4]\n",
      "{'DP': 28, 'VDB': 3.7972e-05, 'SGB': -0.692976, 'MQSB': 0.997478, 'MQ0F': 0.0357143, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 22, 4], 'MQ': 34, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=103040, REF=A, ALT=[G])\n",
      "20\n",
      "[1, 0, 8, 7]\n",
      "{'DP': 20, 'VDB': 0.999721, 'SGB': -0.688148, 'RPB': 1.0, 'MQB': 1.0, 'MQSB': 0.603142, 'BQB': 1.0, 'MQ0F': 0.05, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [1, 0, 8, 7], 'MQ': 31, 'FQ': -999.0, 'PV4': [1.0, 1.0, 1.0, 0.398594]}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=103082, REF=C, ALT=[T, A])\n",
      "27\n",
      "[0, 0, 9, 10]\n",
      "{'DP': 27, 'VDB': 0.909411, 'SGB': -0.69168, 'MQSB': 0.214203, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 9, 10], 'MQ': 36, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=103142, REF=T, ALT=[A])\n",
      "23\n",
      "[0, 0, 16, 5]\n",
      "{'DP': 23, 'VDB': 0.000213448, 'SGB': -0.692352, 'MQSB': 0.488331, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 16, 5], 'MQ': 38, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=103184, REF=C, ALT=[T, A])\n",
      "29\n",
      "[0, 0, 4, 20]\n",
      "{'DP': 29, 'VDB': 2.78429e-07, 'SGB': -0.692831, 'MQSB': 0.981425, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 4, 20], 'MQ': 34, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=105785, REF=T, ALT=[C, A])\n",
      "11\n",
      "[0, 0, 8, 2]\n",
      "{'DP': 11, 'VDB': 0.42619, 'SGB': -0.670168, 'MQSB': 0.875, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 8, 2], 'MQ': 42, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=105869, REF=G, ALT=[A])\n",
      "5\n",
      "[0, 0, 1, 3]\n",
      "{'DP': 5, 'VDB': 0.459448, 'SGB': -0.556411, 'MQSB': 1.0, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 1, 3], 'MQ': 30, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=106719, REF=GAAAAAAAA, ALT=[GAAAAAAAAA, GAAAAAAAAAA])\n",
      "23\n",
      "[0, 0, 7, 6]\n",
      "{'INDEL': True, 'IDV': 17, 'IMF': 0.73913, 'DP': 23, 'VDB': 0.125181, 'SGB': -0.683931, 'MQSB': 0.828239, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 7, 6], 'MQ': 31, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=107202, REF=T, ALT=[C, A, G])\n",
      "24\n",
      "[0, 0, 13, 6]\n",
      "{'DP': 24, 'VDB': 0.877721, 'SGB': -0.69168, 'MQSB': 0.0903697, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 13, 6], 'MQ': 38, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=108119, REF=T, ALT=[C])\n",
      "48\n",
      "[0, 0, 12, 31]\n",
      "{'DP': 48, 'VDB': 0.00287818, 'SGB': -0.693146, 'MQSB': 0.0353237, 'MQ0F': 0.0208333, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 12, 31], 'MQ': 34, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=108555, REF=C, ALT=[T])\n",
      "38\n",
      "[0, 0, 24, 10]\n",
      "{'DP': 38, 'VDB': 0.43848, 'SGB': -0.693132, 'MQSB': 0.965605, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 24, 10], 'MQ': 38, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=112619, REF=A, ALT=[C, G])\n",
      "31\n",
      "[0, 0, 6, 16]\n",
      "{'DP': 31, 'VDB': 0.0662458, 'SGB': -0.692562, 'MQSB': 0.989189, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 6, 16], 'MQ': 38, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=112721, REF=T, ALT=[C])\n",
      "21\n",
      "[0, 0, 11, 10]\n",
      "{'DP': 21, 'VDB': 0.000486641, 'SGB': -0.692352, 'MQSB': 0.780412, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 11, 10], 'MQ': 36, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=114556, REF=C, ALT=[A])\n",
      "27\n",
      "[0, 1, 3, 23]\n",
      "{'DP': 27, 'VDB': 0.214563, 'SGB': -0.692976, 'RPB': 1.0, 'MQB': 1.0, 'MQSB': 0.999256, 'BQB': 1.0, 'MQ0F': 0.037037, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 1, 3, 23], 'MQ': 38, 'FQ': -999.0, 'PV4': [1.0, 1.0, 0.313022, 0.423317]}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=118390, REF=A, ALT=[G, T])\n",
      "14\n",
      "[0, 0, 8, 5]\n",
      "{'DP': 14, 'VDB': 0.282505, 'SGB': -0.683931, 'MQSB': 1.0, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 8, 5], 'MQ': 35, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=118414, REF=T, ALT=[C])\n",
      "21\n",
      "[0, 0, 9, 11]\n",
      "{'DP': 21, 'VDB': 0.200442, 'SGB': -0.692067, 'MQSB': 0.614016, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 9, 11], 'MQ': 37, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=118486, REF=T, ALT=[C, A])\n",
      "25\n",
      "[0, 0, 19, 3]\n",
      "{'DP': 25, 'VDB': 0.386535, 'SGB': -0.692562, 'MQSB': 0.989755, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 19, 3], 'MQ': 37, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=119207, REF=A, ALT=[G])\n",
      "24\n",
      "[0, 0, 16, 7]\n",
      "{'DP': 24, 'VDB': 0.999929, 'SGB': -0.692717, 'MQSB': 0.173774, 'MQ0F': 0.0416667, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 16, 7], 'MQ': 27, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=119210, REF=T, ALT=[C])\n",
      "28\n",
      "[0, 0, 20, 5]\n",
      "{'DP': 28, 'VDB': 0.998507, 'SGB': -0.692914, 'MQSB': 0.907102, 'MQ0F': 0.0357143, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 20, 5], 'MQ': 28, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=119285, REF=A, ALT=[G])\n",
      "39\n",
      "[0, 0, 7, 25]\n",
      "{'DP': 39, 'VDB': 0.224114, 'SGB': -0.69312, 'MQSB': 0.380415, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 7, 25], 'MQ': 39, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=119323, REF=CTTT, ALT=[CTTTT])\n",
      "54\n",
      "[0, 0, 11, 29]\n",
      "{'INDEL': True, 'IDV': 38, 'IMF': 0.703704, 'DP': 54, 'VDB': 0.669979, 'SGB': -0.693145, 'MQSB': 0.895642, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 11, 29], 'MQ': 32, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=119503, REF=C, ALT=[T, A, G])\n",
      "22\n",
      "[0, 0, 7, 5]\n",
      "{'DP': 22, 'VDB': 0.352232, 'SGB': -0.680642, 'MQSB': 0.896474, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 7, 5], 'MQ': 24, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=119506, REF=T, ALT=[C])\n",
      "18\n",
      "[0, 0, 7, 5]\n",
      "{'DP': 18, 'VDB': 0.298917, 'SGB': -0.680642, 'MQSB': 0.896474, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 7, 5], 'MQ': 24, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=119533, REF=C, ALT=[T])\n",
      "8\n",
      "[0, 0, 2, 4]\n",
      "{'DP': 8, 'VDB': 0.0697235, 'SGB': -0.616816, 'MQSB': 0.375, 'MQ0F': 0.125, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 2, 4], 'MQ': 17, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=119536, REF=G, ALT=[A])\n",
      "7\n",
      "[0, 0, 3, 4]\n",
      "{'DP': 7, 'VDB': 0.308783, 'SGB': -0.636426, 'MQSB': 1.01283, 'MQ0F': 0.142857, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 3, 4], 'MQ': 16, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=119601, REF=C, ALT=[T])\n",
      "13\n",
      "[0, 0, 5, 5]\n",
      "{'DP': 13, 'VDB': 0.887186, 'SGB': -0.670168, 'MQSB': 0.761877, 'MQ0F': 0.384615, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 5, 5], 'MQ': 28, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=119647, REF=T, ALT=[G, C])\n",
      "27\n",
      "[0, 0, 4, 22]\n",
      "{'DP': 27, 'VDB': 0.0244427, 'SGB': -0.692976, 'MQSB': 0.984341, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 4, 22], 'MQ': 38, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=119724, REF=T, ALT=[C, G])\n",
      "10\n",
      "[0, 1, 3, 3]\n",
      "{'DP': 10, 'VDB': 0.954032, 'SGB': -0.616816, 'RPB': 1.0, 'MQB': 1.0, 'MQSB': 1.01283, 'BQB': 1.0, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 1, 3, 3], 'MQ': 34, 'FQ': -999.0, 'PV4': [1.0, 1.0, 1.0, 1.0]}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=119763, REF=T, ALT=[C, G])\n",
      "15\n",
      "[0, 0, 7, 8]\n",
      "{'DP': 15, 'VDB': 1.38418e-05, 'SGB': -0.688148, 'MQSB': 0.97357, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 7, 8], 'MQ': 40, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=119802, REF=T, ALT=[A])\n",
      "17\n",
      "[0, 1, 6, 5]\n",
      "{'DP': 17, 'VDB': 0.00537451, 'SGB': -0.676189, 'RPB': 1.0, 'MQB': 1.0, 'MQSB': 0.660716, 'BQB': 1.0, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 1, 6, 5], 'MQ': 28, 'FQ': -999.0, 'PV4': [1.0, 1.0, 0.107949, 0.49018]}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=119829, REF=C, ALT=[T, A])\n",
      "18\n",
      "[0, 0, 7, 7]\n",
      "{'DP': 18, 'VDB': 0.000104818, 'SGB': -0.686358, 'MQSB': 0.30295, 'MQ0F': 0.0555556, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 7, 7], 'MQ': 22, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=119838, REF=A, ALT=[G])\n",
      "22\n",
      "[1, 0, 5, 13]\n",
      "{'DP': 22, 'VDB': 0.990918, 'SGB': -0.691153, 'RPB': 1.0, 'MQB': 1.0, 'MQSB': 0.373583, 'BQB': 1.0, 'MQ0F': 0.0909091, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [1, 0, 5, 13], 'MQ': 27, 'FQ': -999.0, 'PV4': [0.315789, 1.0, 1.0, 1.0]}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=119888, REF=A, ALT=[C])\n",
      "18\n",
      "[0, 0, 6, 6]\n",
      "{'DP': 18, 'VDB': 0.0607873, 'SGB': -0.680642, 'MQSB': 0.931779, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 6, 6], 'MQ': 36, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=119919, REF=C, ALT=[T, A])\n",
      "20\n",
      "[0, 0, 15, 2]\n",
      "{'DP': 20, 'VDB': 0.0451003, 'SGB': -0.690438, 'MQSB': 0.666667, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 15, 2], 'MQ': 39, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=120029, REF=A, ALT=[G])\n",
      "25\n",
      "[0, 0, 13, 9]\n",
      "{'DP': 25, 'VDB': 0.614429, 'SGB': -0.692562, 'MQSB': 0.428345, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 13, 9], 'MQ': 33, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=120057, REF=A, ALT=[G])\n",
      "29\n",
      "[0, 0, 8, 20]\n",
      "{'DP': 29, 'VDB': 0.00303926, 'SGB': -0.693054, 'MQSB': 0.954515, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 8, 20], 'MQ': 36, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=120320, REF=A, ALT=[G])\n",
      "8\n",
      "[0, 0, 7, 1]\n",
      "{'DP': 8, 'VDB': 0.700218, 'SGB': -0.651104, 'MQSB': 1.0, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 7, 1], 'MQ': 33, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=120347, REF=T, ALT=[C])\n",
      "12\n",
      "[0, 0, 3, 8]\n",
      "{'DP': 12, 'VDB': 0.0228839, 'SGB': -0.676189, 'MQSB': 0.979382, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 3, 8], 'MQ': 37, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=120554, REF=G, ALT=[A, T])\n",
      "23\n",
      "[0, 0, 5, 17]\n",
      "{'DP': 23, 'VDB': 1.30187e-05, 'SGB': -0.692562, 'MQSB': 0.455811, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 5, 17], 'MQ': 40, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=120617, REF=C, ALT=[T])\n",
      "6\n",
      "[0, 0, 2, 4]\n",
      "{'DP': 6, 'VDB': 0.205815, 'SGB': -0.616816, 'MQSB': 1.0, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 2, 4], 'MQ': 12, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=120623, REF=G, ALT=[A])\n",
      "7\n",
      "[0, 0, 2, 4]\n",
      "{'DP': 7, 'VDB': 0.215799, 'SGB': -0.616816, 'MQSB': 1.0, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 2, 4], 'MQ': 12, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=120648, REF=A, ALT=[G, T])\n",
      "20\n",
      "[0, 0, 3, 16]\n",
      "{'DP': 20, 'VDB': 0.196641, 'SGB': -0.69168, 'MQSB': 0.502047, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 3, 16], 'MQ': 35, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=120968, REF=A, ALT=[G])\n",
      "16\n",
      "[0, 0, 9, 3]\n",
      "{'DP': 16, 'VDB': 0.00185177, 'SGB': -0.680642, 'MQSB': 0.151887, 'MQ0F': 0.1875, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 9, 3], 'MQ': 30, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=120986, REF=T, ALT=[C])\n",
      "7\n",
      "[0, 0, 2, 2]\n",
      "{'DP': 7, 'VDB': 0.00911214, 'SGB': -0.556411, 'MQSB': 0.0, 'MQ0F': 0.142857, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 2, 2], 'MQ': 17, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=120995, REF=C, ALT=[T])\n",
      "13\n",
      "[0, 0, 4, 8]\n",
      "{'DP': 13, 'VDB': 2.73933e-05, 'SGB': -0.680642, 'MQSB': 0.543691, 'MQ0F': 0.0769231, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 4, 8], 'MQ': 31, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=121058, REF=A, ALT=[G])\n",
      "21\n",
      "[0, 0, 15, 4]\n",
      "{'DP': 21, 'VDB': 0.547624, 'SGB': -0.69168, 'MQSB': 0.923116, 'MQ0F': 0.047619, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 15, 4], 'MQ': 33, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=121090, REF=C, ALT=[T, G])\n",
      "20\n",
      "[0, 0, 7, 10]\n",
      "{'DP': 20, 'VDB': 0.27262, 'SGB': -0.690438, 'MQSB': 0.981133, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 7, 10], 'MQ': 38, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=121457, REF=G, ALT=[T])\n",
      "15\n",
      "[0, 0, 9, 5]\n",
      "{'DP': 15, 'VDB': 0.359018, 'SGB': -0.686358, 'MQSB': 0.222635, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 9, 5], 'MQ': 21, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=121460, REF=C, ALT=[G])\n",
      "20\n",
      "[0, 3, 11, 4]\n",
      "{'DP': 20, 'VDB': 0.818589, 'SGB': -0.688148, 'RPB': 0.97232, 'MQB': 0.0793939, 'MQSB': 0.500025, 'BQB': 0.206192, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 3, 11, 4], 'MQ': 24, 'FQ': -999.0, 'PV4': [0.0428922, 1.0, 0.0134392, 0.101741]}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=121493, REF=G, ALT=[T])\n",
      "12\n",
      "[0, 0, 7, 4]\n",
      "{'DP': 12, 'VDB': 0.593059, 'SGB': -0.676189, 'MQSB': 0.562708, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 7, 4], 'MQ': 30, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=121529, REF=C, ALT=[T])\n",
      "14\n",
      "[0, 0, 6, 2]\n",
      "{'DP': 14, 'VDB': 0.00238612, 'SGB': -0.651104, 'MQSB': 0.5, 'MQ0F': 0.214286, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 6, 2], 'MQ': 19, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=121532, REF=G, ALT=[C])\n",
      "10\n",
      "[0, 0, 4, 1]\n",
      "{'DP': 10, 'VDB': 0.0180471, 'SGB': -0.590765, 'MQSB': 1.0, 'MQ0F': 0.3, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 4, 1], 'MQ': 12, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=121562, REF=G, ALT=[A, T])\n",
      "5\n",
      "[0, 0, 3, 2]\n",
      "{'DP': 5, 'VDB': 0.0868401, 'SGB': -0.590765, 'MQSB': 0.5, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 3, 2], 'MQ': 33, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=121679, REF=A, ALT=[G])\n",
      "28\n"
     ]
    },
    {
     "name": "stdout",
     "output_type": "stream",
     "text": [
      "[0, 0, 21, 3]\n",
      "{'DP': 28, 'VDB': 1.35148e-05, 'SGB': -0.692831, 'MQSB': 0.271496, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 21, 3], 'MQ': 34, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=121712, REF=A, ALT=[G])\n",
      "2\n",
      "[0, 0, 2, 0]\n",
      "{'DP': 2, 'VDB': 0.32, 'SGB': -0.453602, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 2, 0], 'MQ': 41, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=121814, REF=A, ALT=[G])\n",
      "25\n",
      "[0, 0, 11, 10]\n",
      "{'DP': 25, 'VDB': 0.00705183, 'SGB': -0.692352, 'MQSB': 0.447846, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 11, 10], 'MQ': 37, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=121839, REF=GAA, ALT=[G])\n",
      "8\n",
      "[0, 0, 6, 2]\n",
      "{'INDEL': True, 'IDV': 5, 'IMF': 0.625, 'DP': 8, 'VDB': 0.00319992, 'SGB': -0.651104, 'MQSB': 0.25, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 6, 2], 'MQ': 18, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=121840, REF=AAT, ALT=[A])\n",
      "4\n",
      "[0, 0, 3, 0]\n",
      "{'INDEL': True, 'IDV': 3, 'IMF': 0.333333, 'DP': 4, 'VDB': 0.0681597, 'SGB': -0.511536, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 3, 0], 'MQ': 19, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=121882, REF=T, ALT=[C])\n",
      "7\n",
      "[0, 0, 2, 0]\n",
      "{'DP': 7, 'VDB': 0.1, 'SGB': -0.453602, 'MQ0F': 0.142857, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 2, 0], 'MQ': 24, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=121883, REF=C, ALT=[T])\n",
      "7\n",
      "[1, 0, 2, 0]\n",
      "{'DP': 7, 'VDB': 0.1, 'SGB': -0.453602, 'RPB': 1.0, 'MQB': 1.0, 'BQB': 1.0, 'MQ0F': 0.142857, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [1, 0, 2, 0], 'MQ': 20, 'FQ': -999.0, 'PV4': [1.0, 1.0, 1.0, 1.0]}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=121994, REF=A, ALT=[G])\n",
      "6\n",
      "[0, 0, 1, 5]\n",
      "{'DP': 6, 'VDB': 0.0137686, 'SGB': -0.616816, 'MQSB': 1.0, 'MQ0F': 0.333333, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 1, 5], 'MQ': 19, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=121999, REF=G, ALT=[A])\n",
      "11\n",
      "[0, 0, 3, 5]\n",
      "{'DP': 11, 'VDB': 0.00653337, 'SGB': -0.651104, 'MQSB': 0.150134, 'MQ0F': 0.181818, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 3, 5], 'MQ': 26, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=122137, REF=A, ALT=[G])\n",
      "33\n",
      "[0, 0, 25, 8]\n",
      "{'DP': 33, 'VDB': 0.127077, 'SGB': -0.693127, 'MQSB': 0.957686, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 25, 8], 'MQ': 39, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=122335, REF=A, ALT=[G, T])\n",
      "44\n",
      "[0, 0, 9, 28]\n",
      "{'DP': 44, 'VDB': 0.922625, 'SGB': -0.693141, 'MQSB': 0.834371, 'MQ0F': 0.0909091, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 9, 28], 'MQ': 27, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=122338, REF=C, ALT=[T, A])\n",
      "37\n",
      "[0, 0, 7, 22]\n",
      "{'DP': 37, 'VDB': 0.646097, 'SGB': -0.693079, 'MQSB': 0.866597, 'MQ0F': 0.0810811, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 7, 22], 'MQ': 24, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=124069, REF=C, ALT=[T, A])\n",
      "31\n",
      "[0, 0, 12, 19]\n",
      "{'DP': 31, 'VDB': 0.442171, 'SGB': -0.69311, 'MQSB': 0.259528, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 12, 19], 'MQ': 34, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=124099, REF=G, ALT=[A])\n",
      "38\n",
      "[0, 0, 16, 14]\n",
      "{'DP': 38, 'VDB': 0.347452, 'SGB': -0.693097, 'MQSB': 0.909135, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 16, 14], 'MQ': 38, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=124137, REF=TAAAAAAAA, ALT=[TAAAAAAA])\n",
      "43\n",
      "[0, 0, 0, 18]\n",
      "{'INDEL': True, 'IDV': 27, 'IMF': 0.627907, 'DP': 43, 'VDB': 0.283434, 'SGB': -0.691153, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 0, 18], 'MQ': 34, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=124253, REF=T, ALT=[A])\n",
      "45\n",
      "[0, 1, 16, 14]\n",
      "{'DP': 45, 'VDB': 8.59705e-05, 'SGB': -0.693097, 'RPB': 1.0, 'MQB': 1.0, 'MQSB': 0.3946, 'BQB': 1.0, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 1, 16, 14], 'MQ': 37, 'FQ': -999.0, 'PV4': [0.483871, 1.0, 0.263672, 0.39943]}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=124274, REF=T, ALT=[C])\n",
      "4\n",
      "[0, 0, 2, 2]\n",
      "{'DP': 4, 'VDB': 0.836469, 'SGB': -0.556411, 'MQSB': 0.0, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 2, 2], 'MQ': 31, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=124391, REF=G, ALT=[A])\n",
      "38\n",
      "[0, 0, 20, 16]\n",
      "{'DP': 38, 'VDB': 0.00361445, 'SGB': -0.693139, 'MQSB': 0.0960157, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 20, 16], 'MQ': 39, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=124445, REF=G, ALT=[A])\n",
      "51\n",
      "[1, 0, 39, 8]\n",
      "{'DP': 51, 'VDB': 0.0868929, 'SGB': -0.693147, 'RPB': 1.0, 'MQB': 1.0, 'MQSB': 0.927743, 'BQB': 1.0, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [1, 0, 39, 8], 'MQ': 36, 'FQ': -999.0, 'PV4': [1.0, 1.0, 1.0, 1.0]}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=124506, REF=T, ALT=[C, A])\n",
      "18\n",
      "[0, 0, 12, 6]\n",
      "{'DP': 18, 'VDB': 0.611318, 'SGB': -0.691153, 'MQSB': 0.728414, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 12, 6], 'MQ': 32, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=124523, REF=C, ALT=[T, G])\n",
      "31\n",
      "[0, 0, 11, 15]\n",
      "{'DP': 31, 'VDB': 0.149234, 'SGB': -0.692976, 'MQSB': 0.0394126, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 11, 15], 'MQ': 34, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=124626, REF=C, ALT=[A])\n",
      "13\n",
      "[0, 0, 4, 7]\n",
      "{'DP': 13, 'VDB': 0.502759, 'SGB': -0.676189, 'MQSB': 0.361741, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 4, 7], 'MQ': 39, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=124645, REF=A, ALT=[G, T])\n",
      "10\n",
      "[0, 0, 2, 8]\n",
      "{'DP': 10, 'VDB': 0.0135058, 'SGB': -0.670168, 'MQSB': 0.875, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 2, 8], 'MQ': 38, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=124934, REF=A, ALT=[G])\n",
      "59\n",
      "[0, 0, 47, 8]\n",
      "{'DP': 59, 'VDB': 7.62902e-05, 'SGB': -0.693147, 'MQSB': 0.994246, 'MQ0F': 0.0338983, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 47, 8], 'MQ': 34, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=124967, REF=T, ALT=[G, C])\n",
      "18\n",
      "[0, 0, 11, 5]\n",
      "{'DP': 18, 'VDB': 0.799629, 'SGB': -0.689466, 'MQSB': 0.878142, 'MQ0F': 0.0555556, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 11, 5], 'MQ': 22, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=124970, REF=A, ALT=[G])\n",
      "22\n",
      "[0, 0, 11, 6]\n",
      "{'DP': 22, 'VDB': 0.333468, 'SGB': -0.690438, 'MQSB': 0.603475, 'MQ0F': 0.0909091, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 11, 6], 'MQ': 21, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=125063, REF=A, ALT=[G])\n",
      "47\n",
      "[0, 0, 27, 18]\n",
      "{'DP': 47, 'VDB': 0.00141528, 'SGB': -0.693147, 'MQSB': 0.685631, 'MQ0F': 0.0212766, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 27, 18], 'MQ': 36, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=125138, REF=T, ALT=[C, G])\n",
      "14\n",
      "[0, 0, 6, 7]\n",
      "{'DP': 14, 'VDB': 0.0189208, 'SGB': -0.683931, 'MQSB': 0.531709, 'MQ0F': 0.0714286, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 6, 7], 'MQ': 37, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=125155, REF=G, ALT=[A])\n",
      "27\n",
      "[0, 0, 1, 21]\n",
      "{'DP': 27, 'VDB': 9.40594e-06, 'SGB': -0.692562, 'MQSB': 1.0, 'MQ0F': 0.111111, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 1, 21], 'MQ': 24, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=125156, REF=G, ALT=[A, T])\n",
      "28\n",
      "[0, 0, 1, 22]\n",
      "{'DP': 28, 'VDB': 8.59447e-06, 'SGB': -0.692717, 'MQSB': 1.0, 'MQ0F': 0.107143, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 1, 22], 'MQ': 24, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=125342, REF=A, ALT=[G])\n",
      "49\n",
      "[0, 0, 15, 27]\n",
      "{'DP': 49, 'VDB': 0.0635029, 'SGB': -0.693146, 'MQSB': 0.44467, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 15, 27], 'MQ': 35, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=125393, REF=C, ALT=[T])\n",
      "28\n",
      "[0, 0, 12, 11]\n",
      "{'DP': 28, 'VDB': 0.988596, 'SGB': -0.692717, 'MQSB': 0.119376, 'MQ0F': 0.0357143, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 12, 11], 'MQ': 34, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=125532, REF=T, ALT=[C])\n",
      "35\n",
      "[0, 0, 11, 19]\n",
      "{'DP': 35, 'VDB': 0.0549936, 'SGB': -0.693097, 'MQSB': 0.875155, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 11, 19], 'MQ': 38, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=126287, REF=G, ALT=[A])\n",
      "39\n",
      "[0, 0, 17, 19]\n",
      "{'DP': 39, 'VDB': 0.116573, 'SGB': -0.693139, 'MQSB': 0.395229, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 17, 19], 'MQ': 37, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=126317, REF=C, ALT=[T, A, G])\n",
      "18\n",
      "[0, 0, 8, 10]\n",
      "{'DP': 18, 'VDB': 5.09429e-06, 'SGB': -0.691153, 'MQSB': 0.240508, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 8, 10], 'MQ': 31, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=126341, REF=T, ALT=[A])\n",
      "7\n",
      "[0, 0, 3, 3]\n",
      "{'DP': 7, 'VDB': 0.302039, 'SGB': -0.616816, 'MQSB': 0.574341, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 3, 3], 'MQ': 31, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=126455, REF=G, ALT=[A])\n",
      "19\n",
      "[0, 0, 7, 11]\n",
      "{'DP': 19, 'VDB': 0.00522, 'SGB': -0.691153, 'MQSB': 0.373326, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 7, 11], 'MQ': 38, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=126503, REF=T, ALT=[C, A])\n",
      "23\n",
      "[0, 0, 14, 4]\n",
      "{'DP': 23, 'VDB': 0.754022, 'SGB': -0.691153, 'MQSB': 0.843173, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 14, 4], 'MQ': 40, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=126575, REF=G, ALT=[A, C])\n",
      "10\n",
      "[0, 0, 4, 5]\n",
      "{'DP': 10, 'VDB': 0.0149639, 'SGB': -0.662043, 'MQSB': 0.89338, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 4, 5], 'MQ': 37, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=126602, REF=A, ALT=[C])\n",
      "22\n",
      "[0, 0, 7, 15]\n",
      "{'DP': 22, 'VDB': 0.155085, 'SGB': -0.692562, 'MQSB': 0.447102, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 7, 15], 'MQ': 37, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=126725, REF=C, ALT=[T])\n",
      "25\n",
      "[0, 0, 16, 5]\n",
      "{'DP': 25, 'VDB': 0.997375, 'SGB': -0.692352, 'MQSB': 0.846161, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 16, 5], 'MQ': 38, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=126854, REF=T, ALT=[A, C])\n",
      "34\n",
      "[0, 0, 24, 10]\n",
      "{'DP': 34, 'VDB': 0.683553, 'SGB': -0.693132, 'MQSB': 0.396248, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 24, 10], 'MQ': 37, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=127079, REF=T, ALT=[C])\n",
      "12\n",
      "[0, 0, 0, 7]\n",
      "{'DP': 12, 'VDB': 0.911144, 'SGB': -0.636426, 'MQ0F': 0.0833333, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 0, 7], 'MQ': 16, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=127085, REF=T, ALT=[C])\n",
      "18\n",
      "[0, 0, 0, 17]\n",
      "{'DP': 18, 'VDB': 0.632343, 'SGB': -0.690438, 'MQ0F': 0.111111, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 0, 17], 'MQ': 18, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=127094, REF=A, ALT=[G, C])\n",
      "32\n",
      "[0, 0, 4, 22]\n",
      "{'DP': 32, 'VDB': 5.27628e-05, 'SGB': -0.692976, 'MQSB': 0.382806, 'MQ0F': 0.0625, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 4, 22], 'MQ': 28, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=127517, REF=T, ALT=[C])\n",
      "8\n",
      "[0, 0, 2, 5]\n",
      "{'DP': 8, 'VDB': 0.626747, 'SGB': -0.636426, 'MQSB': 0.3, 'MQ0F': 0.125, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 2, 5], 'MQ': 31, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=127616, REF=T, ALT=[C])\n",
      "12\n",
      "[0, 0, 11, 1]\n",
      "{'DP': 12, 'VDB': 0.102893, 'SGB': -0.680642, 'MQSB': 1.0, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 11, 1], 'MQ': 36, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=127686, REF=G, ALT=[T, A])\n",
      "23\n",
      "[0, 0, 15, 2]\n",
      "{'DP': 23, 'VDB': 0.673796, 'SGB': -0.690438, 'MQSB': 0.433333, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 15, 2], 'MQ': 38, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=127829, REF=T, ALT=[C])\n",
      "17\n",
      "[0, 0, 8, 9]\n",
      "{'DP': 17, 'VDB': 0.223209, 'SGB': -0.690438, 'MQSB': 0.797039, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 8, 9], 'MQ': 39, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=127853, REF=T, ALT=[C, G])\n",
      "15\n",
      "[0, 0, 8, 7]\n",
      "{'DP': 15, 'VDB': 0.152765, 'SGB': -0.688148, 'MQSB': 0.616426, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 8, 7], 'MQ': 37, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=127892, REF=A, ALT=[G])\n",
      "5\n",
      "[0, 0, 3, 1]\n",
      "{'DP': 5, 'VDB': 0.124686, 'SGB': -0.556411, 'MQSB': 1.0, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 3, 1], 'MQ': 28, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=127905, REF=A, ALT=[C])\n",
      "18\n",
      "[0, 0, 1, 16]\n",
      "{'DP': 18, 'VDB': 2.6302e-05, 'SGB': -0.690438, 'MQSB': 1.0, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 1, 16], 'MQ': 35, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=128004, REF=GATAATAATA, ALT=[GATAATA])\n",
      "17\n",
      "[0, 0, 6, 4]\n",
      "{'INDEL': True, 'IDV': 8, 'IMF': 0.470588, 'DP': 17, 'VDB': 0.0978464, 'SGB': -0.670168, 'MQSB': 0.279932, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 6, 4], 'MQ': 9, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=128040, REF=GATAATA, ALT=[GATAATAATA])\n",
      "21\n",
      "[0, 0, 9, 2]\n",
      "{'INDEL': True, 'IDV': 12, 'IMF': 0.571429, 'DP': 21, 'VDB': 0.0169271, 'SGB': -0.676189, 'MQSB': 0.888889, 'MQ0F': 0.190476, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 9, 2], 'MQ': 10, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=128096, REF=C, ALT=[A])\n",
      "13\n",
      "[0, 0, 9, 4]\n",
      "{'DP': 13, 'VDB': 0.970438, 'SGB': -0.683931, 'MQSB': 0.826565, 'MQ0F': 0.461538, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 9, 4], 'MQ': 24, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=128100, REF=A, ALT=[G])\n",
      "14\n",
      "[0, 0, 7, 4]\n",
      "{'DP': 14, 'VDB': 0.238643, 'SGB': -0.676189, 'MQSB': 0.200967, 'MQ0F': 0.642857, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 7, 4], 'MQ': 16, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=128171, REF=C, ALT=[T])\n",
      "10\n",
      "[0, 0, 3, 5]\n",
      "{'DP': 10, 'VDB': 0.603537, 'SGB': -0.651104, 'MQSB': 0.450401, 'MQ0F': 0.1, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 3, 5], 'MQ': 25, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=128182, REF=C, ALT=[T])\n",
      "18\n",
      "[0, 0, 5, 13]\n",
      "{'DP': 18, 'VDB': 0.226676, 'SGB': -0.691153, 'MQSB': 0.311235, 'MQ0F': 0.0555556, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 5, 13], 'MQ': 30, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=128213, REF=C, ALT=[T])\n",
      "21\n",
      "[0, 0, 2, 11]\n",
      "{'DP': 21, 'VDB': 0.74314, 'SGB': -0.683931, 'MQSB': 0.272727, 'MQ0F': 0.047619, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 2, 11], 'MQ': 36, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=128279, REF=G, ALT=[A, T])\n",
      "26\n",
      "[1, 0, 11, 9]\n",
      "{'DP': 26, 'VDB': 0.00424252, 'SGB': -0.692067, 'RPB': 1.0, 'MQB': 1.0, 'MQSB': 0.27848, 'BQB': 1.0, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [1, 0, 11, 9], 'MQ': 35, 'FQ': -999.0, 'PV4': [1.0, 1.0, 1.0, 1.0]}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=128324, REF=C, ALT=[T, A, G])\n",
      "21\n",
      "[0, 0, 14, 4]\n",
      "{'DP': 21, 'VDB': 0.974022, 'SGB': -0.691153, 'MQSB': 0.994377, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 14, 4], 'MQ': 30, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=128326, REF=A, ALT=[C])\n",
      "25\n",
      "[0, 0, 14, 4]\n",
      "{'DP': 25, 'VDB': 0.974663, 'SGB': -0.691153, 'MQSB': 0.994377, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 14, 4], 'MQ': 30, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=128396, REF=G, ALT=[A])\n",
      "21\n",
      "[0, 0, 5, 14]\n",
      "{'DP': 21, 'VDB': 0.05403, 'SGB': -0.69168, 'MQSB': 0.933727, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 5, 14], 'MQ': 37, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=128438, REF=G, ALT=[A, T])\n",
      "28\n",
      "[0, 0, 13, 15]\n",
      "{'DP': 28, 'VDB': 0.000989662, 'SGB': -0.693054, 'MQSB': 0.116654, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 13, 15], 'MQ': 36, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=128453, REF=G, ALT=[A])\n",
      "8\n",
      "[0, 0, 1, 1]\n",
      "{'DP': 8, 'VDB': 0.72, 'SGB': -0.453602, 'MQSB': 1.0, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 1, 1], 'MQ': 40, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=128517, REF=A, ALT=[G])\n",
      "27\n",
      "[0, 0, 15, 9]\n",
      "{'DP': 27, 'VDB': 0.0192291, 'SGB': -0.692831, 'MQSB': 0.999556, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 15, 9], 'MQ': 39, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=128585, REF=T, ALT=[C])\n",
      "27\n",
      "[0, 0, 20, 5]\n",
      "{'DP': 27, 'VDB': 3.41428e-06, 'SGB': -0.692914, 'MQSB': 0.94394, 'MQ0F': 0.0740741, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 20, 5], 'MQ': 32, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=128599, REF=A, ALT=[G])\n",
      "7\n",
      "[0, 0, 2, 1]\n",
      "{'DP': 7, 'VDB': 0.681436, 'SGB': -0.511536, 'MQSB': 1.0, 'MQ0F': 0.285714, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 2, 1], 'MQ': 20, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=128600, REF=G, ALT=[T])\n",
      "7\n",
      "[0, 0, 2, 1]\n",
      "{'DP': 7, 'VDB': 0.681436, 'SGB': -0.511536, 'MQSB': 1.0, 'MQ0F': 0.285714, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 2, 1], 'MQ': 20, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=128699, REF=T, ALT=[A])\n",
      "22\n",
      "[1, 0, 6, 13]\n",
      "{'DP': 22, 'VDB': 0.465123, 'SGB': -0.69168, 'RPB': 1.0, 'MQB': 1.0, 'MQSB': 0.303044, 'BQB': 1.0, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [1, 0, 6, 13], 'MQ': 36, 'FQ': -999.0, 'PV4': [0.35, 0.0747926, 1.0, 1.0]}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=128769, REF=C, ALT=[A])\n",
      "8\n",
      "[0, 0, 7, 1]\n",
      "{'DP': 8, 'VDB': 0.152401, 'SGB': -0.651104, 'MQSB': 1.0, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 7, 1], 'MQ': 37, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=128789, REF=C, ALT=[T, G])\n",
      "8\n",
      "[0, 0, 5, 2]\n",
      "{'DP': 8, 'VDB': 0.702808, 'SGB': -0.636426, 'MQSB': 0.2, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 5, 2], 'MQ': 41, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=128825, REF=C, ALT=[T, G])\n",
      "13\n",
      "[0, 0, 5, 8]\n",
      "{'DP': 13, 'VDB': 0.659814, 'SGB': -0.683931, 'MQSB': 0.765017, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 5, 8], 'MQ': 38, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=128877, REF=C, ALT=[T])\n",
      "27\n",
      "[0, 0, 13, 8]\n",
      "{'DP': 27, 'VDB': 0.00147284, 'SGB': -0.692352, 'MQSB': 0.936543, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 13, 8], 'MQ': 39, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=129002, REF=T, ALT=[G])\n",
      "37\n",
      "[0, 1, 20, 12]\n",
      "{'DP': 37, 'VDB': 0.912501, 'SGB': -0.69312, 'RPB': 1.0, 'MQB': 1.0, 'MQSB': 0.00362251, 'BQB': 1.0, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 1, 20, 12], 'MQ': 37, 'FQ': -999.0, 'PV4': [0.393939, 1.0, 0.28922, 0.419437]}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=129083, REF=C, ALT=[T, G])\n",
      "32\n",
      "[0, 0, 22, 6]\n",
      "{'DP': 32, 'VDB': 0.813848, 'SGB': -0.693054, 'MQSB': 0.652644, 'MQ0F': 0.03125, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 22, 6], 'MQ': 28, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=129089, REF=T, ALT=[C, A])\n",
      "25\n",
      "[0, 0, 12, 11]\n",
      "{'DP': 25, 'VDB': 0.948165, 'SGB': -0.692717, 'MQSB': 0.999527, 'MQ0F': 0.08, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 12, 11], 'MQ': 22, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=129168, REF=T, ALT=[C])\n",
      "23\n",
      "[0, 0, 2, 11]\n",
      "{'DP': 23, 'VDB': 2.29934e-06, 'SGB': -0.683931, 'MQSB': 0.727273, 'MQ0F': 0.0434783, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 2, 11], 'MQ': 23, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=129169, REF=A, ALT=[G])\n",
      "23\n",
      "[0, 0, 2, 11]\n",
      "{'DP': 23, 'VDB': 1.79355e-06, 'SGB': -0.683931, 'MQSB': 0.727273, 'MQ0F': 0.0434783, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 2, 11], 'MQ': 23, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=129179, REF=A, ALT=[C])\n",
      "5\n",
      "[0, 0, 5, 0]\n",
      "{'DP': 5, 'VDB': 0.548401, 'SGB': -0.590765, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 5, 0], 'MQ': 37, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=129251, REF=G, ALT=[A, T])\n",
      "20\n",
      "[0, 0, 2, 15]\n",
      "{'DP': 20, 'VDB': 0.0203068, 'SGB': -0.690438, 'MQSB': 0.133333, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 2, 15], 'MQ': 38, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=129356, REF=A, ALT=[G])\n",
      "16\n",
      "[0, 0, 12, 3]\n",
      "{'DP': 16, 'VDB': 0.271244, 'SGB': -0.688148, 'MQSB': 0.976835, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 12, 3], 'MQ': 38, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=129457, REF=G, ALT=[A])\n",
      "10\n",
      "[0, 0, 2, 8]\n",
      "{'DP': 10, 'VDB': 0.104744, 'SGB': -0.670168, 'MQSB': 0.625, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 2, 8], 'MQ': 28, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=129460, REF=C, ALT=[A])\n",
      "12\n",
      "[0, 1, 3, 8]\n",
      "{'DP': 12, 'VDB': 0.24616, 'SGB': -0.676189, 'RPB': 1.0, 'MQB': 1.0, 'MQSB': 0.290822, 'BQB': 1.0, 'MQ0F': 0.0833333, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 1, 3, 8], 'MQ': 29, 'FQ': -999.0, 'PV4': [1.0, 1.0, 0.138161, 1.0]}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=129489, REF=T, ALT=[C])\n",
      "14\n",
      "[0, 0, 10, 3]\n",
      "{'DP': 14, 'VDB': 0.00525213, 'SGB': -0.683931, 'MQSB': 0.496585, 'MQ0F': 0.214286, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 10, 3], 'MQ': 15, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=129493, REF=G, ALT=[A])\n",
      "13\n",
      "[0, 0, 10, 3]\n",
      "{'DP': 13, 'VDB': 0.0134027, 'SGB': -0.683931, 'MQSB': 0.496585, 'MQ0F': 0.230769, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 10, 3], 'MQ': 12, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=129500, REF=G, ALT=[A])\n",
      "15\n",
      "[0, 0, 11, 2]\n",
      "{'DP': 15, 'VDB': 0.283476, 'SGB': -0.683931, 'MQSB': 0.545455, 'MQ0F': 0.2, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 11, 2], 'MQ': 17, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=129634, REF=A, ALT=[G])\n",
      "21\n",
      "[0, 1, 7, 5]\n",
      "{'DP': 21, 'VDB': 0.0019124, 'SGB': -0.680642, 'RPB': 1.0, 'MQB': 1.0, 'MQSB': 0.31698, 'BQB': 1.0, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 1, 7, 5], 'MQ': 24, 'FQ': -999.0, 'PV4': [0.461538, 1.0, 0.0418156, 1.0]}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=129640, REF=G, ALT=[A])\n",
      "17\n",
      "[0, 0, 7, 5]\n",
      "{'DP': 17, 'VDB': 0.0019124, 'SGB': -0.680642, 'MQSB': 0.506703, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 7, 5], 'MQ': 23, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=129683, REF=A, ALT=[G])\n",
      "22\n",
      "[0, 0, 7, 14]\n",
      "{'DP': 22, 'VDB': 0.000837069, 'SGB': -0.692352, 'MQSB': 0.512426, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 7, 14], 'MQ': 39, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=129779, REF=T, ALT=[A])\n",
      "4\n",
      "[0, 0, 0, 2]\n",
      "{'DP': 4, 'VDB': 0.42, 'SGB': -0.453602, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 0, 2], 'MQ': 34, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=129962, REF=C, ALT=[A])\n",
      "13\n",
      "[0, 2, 1, 9]\n",
      "{'DP': 13, 'VDB': 0.0654841, 'SGB': -0.670168, 'RPB': 0.6, 'MQB': 0.95, 'MQSB': 1.0, 'BQB': 0.3, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 2, 1, 9], 'MQ': 37, 'FQ': -999.0, 'PV4': [1.0, 1.0, 0.25197, 0.0999301]}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=130044, REF=T, ALT=[C, A, G])\n",
      "22\n",
      "[0, 0, 17, 5]\n",
      "{'DP': 22, 'VDB': 0.0156311, 'SGB': -0.692562, 'MQSB': 0.993118, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 17, 5], 'MQ': 39, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=130083, REF=C, ALT=[T, G])\n",
      "16\n",
      "[0, 0, 7, 8]\n",
      "{'DP': 16, 'VDB': 0.00046982, 'SGB': -0.688148, 'MQSB': 0.381255, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 7, 8], 'MQ': 39, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=130105, REF=A, ALT=[G])\n",
      "3\n",
      "[0, 0, 2, 1]\n",
      "{'DP': 3, 'VDB': 0.0681597, 'SGB': -0.511536, 'MQSB': 1.0, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 2, 1], 'MQ': 40, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=130128, REF=C, ALT=[T])\n",
      "22\n",
      "[0, 0, 14, 8]\n",
      "{'DP': 22, 'VDB': 1.11423e-05, 'SGB': -0.692562, 'MQSB': 0.183053, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 14, 8], 'MQ': 40, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=130216, REF=G, ALT=[A])\n",
      "53\n",
      "[0, 0, 10, 37]\n",
      "{'DP': 53, 'VDB': 0.189004, 'SGB': -0.693147, 'MQSB': 0.975887, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 10, 37], 'MQ': 37, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=130245, REF=T, ALT=[C])\n",
      "24\n",
      "[0, 0, 5, 18]\n",
      "{'DP': 24, 'VDB': 0.887648, 'SGB': -0.692717, 'MQSB': 0.0531824, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 5, 18], 'MQ': 36, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=130354, REF=C, ALT=[T, A])\n",
      "30\n",
      "[0, 0, 24, 5]\n",
      "{'DP': 30, 'VDB': 0.707441, 'SGB': -0.693079, 'MQSB': 0.886551, 'MQ0F': 0.0333333, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 24, 5], 'MQ': 36, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=130423, REF=G, ALT=[T])\n",
      "23\n",
      "[0, 0, 7, 15]\n",
      "{'DP': 23, 'VDB': 0.848827, 'SGB': -0.692562, 'MQSB': 0.990111, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 7, 15], 'MQ': 37, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=130590, REF=A, ALT=[G])\n",
      "5\n",
      "[0, 0, 1, 3]\n",
      "{'DP': 5, 'VDB': 0.789602, 'SGB': -0.556411, 'MQSB': 1.0, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 1, 3], 'MQ': 27, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=130602, REF=C, ALT=[T])\n",
      "3\n",
      "[0, 0, 1, 2]\n",
      "{'DP': 3, 'VDB': 0.309755, 'SGB': -0.511536, 'MQSB': 1.0, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 1, 2], 'MQ': 27, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=130683, REF=T, ALT=[C])\n",
      "40\n",
      "[0, 0, 19, 14]\n",
      "{'DP': 40, 'VDB': 0.751844, 'SGB': -0.693127, 'MQSB': 0.953198, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 19, 14], 'MQ': 38, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=130788, REF=C, ALT=[A])\n",
      "11\n",
      "[0, 2, 6, 3]\n",
      "{'DP': 11, 'VDB': 0.0224906, 'SGB': -0.662043, 'RPB': 0.777778, 'MQB': 0.333333, 'MQSB': 0.208021, 'BQB': 0.0, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 2, 6, 3], 'MQ': 41, 'FQ': -999.0, 'PV4': [0.181818, 1.0, 0.0519441, 1.0]}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=130866, REF=A, ALT=[G])\n",
      "16\n",
      "[0, 0, 10, 3]\n",
      "{'DP': 16, 'VDB': 0.781267, 'SGB': -0.683931, 'MQSB': 0.879351, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 10, 3], 'MQ': 32, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=130896, REF=A, ALT=[T])\n",
      "22\n",
      "[0, 0, 14, 7]\n",
      "{'DP': 22, 'VDB': 0.70939, 'SGB': -0.692352, 'MQSB': 0.982757, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 14, 7], 'MQ': 35, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=130954, REF=A, ALT=[G])\n",
      "10\n",
      "[0, 0, 5, 3]\n",
      "{'DP': 10, 'VDB': 0.538025, 'SGB': -0.651104, 'MQSB': 0.900802, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 5, 3], 'MQ': 37, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=131024, REF=C, ALT=[T])\n",
      "36\n",
      "[0, 0, 13, 20]\n",
      "{'DP': 36, 'VDB': 0.535706, 'SGB': -0.693127, 'MQSB': 0.0198339, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 13, 20], 'MQ': 37, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=131103, REF=A, ALT=[G])\n",
      "8\n",
      "[0, 0, 5, 3]\n",
      "{'DP': 8, 'VDB': 0.038698, 'SGB': -0.651104, 'MQSB': 0.750668, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 5, 3], 'MQ': 39, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=131124, REF=A, ALT=[C])\n",
      "5\n",
      "[2, 0, 1, 2]\n",
      "{'DP': 5, 'VDB': 0.192436, 'SGB': -0.511536, 'RPB': 0.0, 'MQB': 0.333333, 'MQSB': 0.0, 'BQB': 0.166667, 'MQ0F': 0.0, 'AF1': 0.503993, 'AC1': 1.0, 'DP4': [2, 0, 1, 2], 'MQ': 39, 'FQ': -999.0, 'PV4': [0.4, 1.0, 0.215727, 0.0320475]}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=131920, REF=C, ALT=[T])\n",
      "9\n",
      "[0, 1, 6, 2]\n",
      "{'DP': 9, 'VDB': 0.901249, 'SGB': -0.651104, 'RPB': 1.0, 'MQB': 1.0, 'MQSB': 0.809011, 'BQB': 1.0, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 1, 6, 2], 'MQ': 37, 'FQ': -999.0, 'PV4': [0.333333, 1.0, 0.247009, 1.0]}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=132076, REF=A, ALT=[C])\n",
      "9\n",
      "[0, 0, 4, 0]\n",
      "{'DP': 9, 'VDB': 0.0238972, 'SGB': -0.556411, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 4, 0], 'MQ': 31, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=132086, REF=A, ALT=[C, G])\n",
      "16\n",
      "[0, 0, 7, 4]\n",
      "{'DP': 16, 'VDB': 1.32041e-05, 'SGB': -0.676189, 'MQSB': 0.361741, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 7, 4], 'MQ': 32, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=132133, REF=C, ALT=[T, A, G])\n",
      "30\n",
      "[0, 0, 23, 7]\n",
      "{'DP': 30, 'VDB': 0.142219, 'SGB': -0.693097, 'MQSB': 0.980949, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 23, 7], 'MQ': 39, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=132213, REF=T, ALT=[G])\n",
      "31\n",
      "[0, 0, 25, 5]\n",
      "{'DP': 31, 'VDB': 0.00302132, 'SGB': -0.693097, 'MQSB': 0.993826, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 25, 5], 'MQ': 38, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=132239, REF=C, ALT=[T, G])\n",
      "20\n",
      "[0, 0, 12, 7]\n",
      "{'DP': 20, 'VDB': 0.188295, 'SGB': -0.69168, 'MQSB': 0.968368, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 12, 7], 'MQ': 36, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=132359, REF=ATTTTTTTT, ALT=[ATTTTTTTTT])\n",
      "19\n",
      "[0, 0, 3, 9]\n",
      "{'INDEL': True, 'IDV': 8, 'IMF': 0.421053, 'DP': 19, 'VDB': 0.378827, 'SGB': -0.680642, 'MQSB': 0.933909, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 3, 9], 'MQ': 31, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=132387, REF=T, ALT=[G])\n",
      "30\n",
      "[0, 0, 3, 24]\n",
      "{'DP': 30, 'VDB': 0.705156, 'SGB': -0.693021, 'MQSB': 0.953497, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 3, 24], 'MQ': 38, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=132768, REF=A, ALT=[C])\n",
      "25\n",
      "[2, 0, 13, 7]\n",
      "{'DP': 25, 'VDB': 1.5713e-05, 'SGB': -0.692067, 'RPB': 0.5, 'MQB': 0.65, 'MQSB': 0.447102, 'BQB': 0.3, 'MQ0F': 0.04, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [2, 0, 13, 7], 'MQ': 33, 'FQ': -999.0, 'PV4': [1.0, 1.0, 1.0, 0.113111]}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=132844, REF=G, ALT=[A])\n",
      "18\n",
      "[0, 0, 11, 6]\n",
      "{'DP': 18, 'VDB': 0.000110097, 'SGB': -0.690438, 'MQSB': 0.980001, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 11, 6], 'MQ': 40, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=132865, REF=A, ALT=[G])\n",
      "2\n",
      "[0, 0, 0, 2]\n",
      "{'DP': 2, 'VDB': 0.42, 'SGB': -0.453602, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 0, 2], 'MQ': 41, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=132917, REF=T, ALT=[G])\n",
      "16\n",
      "[0, 1, 4, 8]\n",
      "{'DP': 16, 'VDB': 0.000408428, 'SGB': -0.680642, 'RPB': 1.0, 'MQB': 1.0, 'MQSB': 0.898397, 'BQB': 1.0, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 1, 4, 8], 'MQ': 37, 'FQ': -999.0, 'PV4': [1.0, 1.0, 0.339625, 0.355402]}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=132946, REF=A, ALT=[G])\n",
      "10\n",
      "[0, 0, 2, 7]\n",
      "{'DP': 10, 'VDB': 0.00724644, 'SGB': -0.662043, 'MQSB': 0.785714, 'MQ0F': 0.1, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 2, 7], 'MQ': 31, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=133055, REF=G, ALT=[A])\n",
      "11\n",
      "[0, 0, 7, 1]\n",
      "{'DP': 11, 'VDB': 0.00610857, 'SGB': -0.651104, 'MQSB': 1.0, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 7, 1], 'MQ': 35, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=133064, REF=T, ALT=[C])\n",
      "10\n",
      "[0, 0, 5, 4]\n",
      "{'DP': 10, 'VDB': 0.746772, 'SGB': -0.662043, 'MQSB': 0.89338, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 5, 4], 'MQ': 32, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=133102, REF=T, ALT=[C])\n",
      "14\n",
      "[0, 0, 9, 4]\n",
      "{'DP': 14, 'VDB': 0.00767807, 'SGB': -0.683931, 'MQSB': 0.826565, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 9, 4], 'MQ': 35, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=133111, REF=G, ALT=[A, C])\n",
      "5\n",
      "[0, 0, 3, 2]\n",
      "{'DP': 5, 'VDB': 0.281015, 'SGB': -0.590765, 'MQSB': 0.666667, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 3, 2], 'MQ': 26, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=133256, REF=A, ALT=[G])\n",
      "11\n",
      "[0, 0, 6, 1]\n",
      "{'DP': 11, 'VDB': 0.734439, 'SGB': -0.636426, 'MQSB': 1.0, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 6, 1], 'MQ': 34, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=133426, REF=G, ALT=[A])\n",
      "35\n",
      "[0, 0, 15, 18]\n",
      "{'DP': 35, 'VDB': 0.110498, 'SGB': -0.693127, 'MQSB': 0.408701, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 15, 18], 'MQ': 41, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=133552, REF=A, ALT=[G])\n",
      "43\n",
      "[0, 0, 8, 35]\n",
      "{'DP': 43, 'VDB': 0.530419, 'SGB': -0.693146, 'MQSB': 0.999878, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 8, 35], 'MQ': 39, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=135394, REF=C, ALT=[T, G])\n",
      "26\n",
      "[1, 0, 16, 9]\n",
      "{'DP': 26, 'VDB': 0.466999, 'SGB': -0.692914, 'RPB': 1.0, 'MQB': 1.0, 'MQSB': 0.689476, 'BQB': 1.0, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [1, 0, 16, 9], 'MQ': 40, 'FQ': -999.0, 'PV4': [1.0, 1.0, 0.352534, 1.0]}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=135471, REF=G, ALT=[A, T])\n",
      "7\n",
      "[0, 0, 0, 7]\n",
      "{'DP': 7, 'VDB': 0.922194, 'SGB': -0.636426, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 0, 7], 'MQ': 36, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=135529, REF=A, ALT=[G])\n",
      "10\n",
      "[0, 0, 7, 3]\n",
      "{'DP': 10, 'VDB': 0.192198, 'SGB': -0.670168, 'MQSB': 0.824834, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 7, 3], 'MQ': 41, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=135971, REF=G, ALT=[A])\n",
      "10\n",
      "[0, 0, 5, 5]\n",
      "{'DP': 10, 'VDB': 0.112814, 'SGB': -0.670168, 'MQSB': 0.904729, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 5, 5], 'MQ': 21, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=135972, REF=A, ALT=[T, G])\n",
      "11\n",
      "[0, 0, 6, 5]\n",
      "{'DP': 11, 'VDB': 0.2216, 'SGB': -0.676189, 'MQSB': 0.742931, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 6, 5], 'MQ': 24, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=136020, REF=A, ALT=[G])\n",
      "3\n",
      "[0, 0, 3, 0]\n",
      "{'DP': 3, 'VDB': 0.235765, 'SGB': -0.511536, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 3, 0], 'MQ': 35, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=136036, REF=T, ALT=[C])\n",
      "2\n",
      "[0, 0, 0, 2]\n",
      "{'DP': 2, 'VDB': 0.22, 'SGB': -0.453602, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 0, 2], 'MQ': 40, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=136089, REF=T, ALT=[C])\n",
      "17\n",
      "[0, 0, 2, 13]\n",
      "{'DP': 17, 'VDB': 0.000438758, 'SGB': -0.688148, 'MQSB': 0.653846, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 2, 13], 'MQ': 37, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=136245, REF=C, ALT=[T, G])\n",
      "16\n",
      "[0, 0, 13, 3]\n",
      "{'DP': 16, 'VDB': 0.622694, 'SGB': -0.689466, 'MQSB': 0.99774, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 13, 3], 'MQ': 39, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=136350, REF=T, ALT=[C])\n",
      "4\n",
      "[0, 0, 1, 3]\n",
      "{'DP': 4, 'VDB': 0.0277111, 'SGB': -0.556411, 'MQSB': 1.0, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 1, 3], 'MQ': 40, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=136375, REF=T, ALT=[C])\n",
      "15\n",
      "[0, 0, 5, 6]\n",
      "{'DP': 15, 'VDB': 0.0868969, 'SGB': -0.676189, 'MQSB': 0.8618, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 5, 6], 'MQ': 35, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=136419, REF=A, ALT=[G])\n",
      "13\n",
      "[0, 0, 8, 2]\n",
      "{'DP': 13, 'VDB': 0.0546694, 'SGB': -0.670168, 'MQSB': 0.25, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 8, 2], 'MQ': 35, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=136441, REF=C, ALT=[T])\n",
      "14\n",
      "[0, 0, 11, 3]\n",
      "{'DP': 14, 'VDB': 0.00204843, 'SGB': -0.686358, 'MQSB': 0.646383, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 11, 3], 'MQ': 41, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=136491, REF=A, ALT=[G])\n",
      "14\n",
      "[0, 0, 1, 9]\n",
      "{'DP': 14, 'VDB': 0.00501405, 'SGB': -0.670168, 'MQSB': 1.0, 'MQ0F': 0.0714286, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 1, 9], 'MQ': 24, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=136494, REF=G, ALT=[A, C, T])\n",
      "17\n",
      "[0, 0, 1, 11]\n",
      "{'DP': 17, 'VDB': 0.00551213, 'SGB': -0.680642, 'MQSB': 1.0, 'MQ0F': 0.0588235, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 1, 11], 'MQ': 25, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=136585, REF=A, ALT=[T, G])\n",
      "21\n",
      "[0, 0, 9, 12]\n",
      "{'DP': 21, 'VDB': 0.0475013, 'SGB': -0.692352, 'MQSB': 0.652615, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 9, 12], 'MQ': 28, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=136593, REF=G, ALT=[A])\n",
      "15\n",
      "[0, 0, 4, 9]\n",
      "{'DP': 15, 'VDB': 0.896961, 'SGB': -0.683931, 'MQSB': 0.304076, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 4, 9], 'MQ': 24, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=136600, REF=C, ALT=[T, G])\n",
      "13\n",
      "[0, 0, 0, 9]\n",
      "{'DP': 13, 'VDB': 0.0308846, 'SGB': -0.662043, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 0, 9], 'MQ': 26, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=136635, REF=C, ALT=[T])\n",
      "11\n",
      "[0, 0, 1, 8]\n",
      "{'DP': 11, 'VDB': 0.000419752, 'SGB': -0.662043, 'MQSB': 1.0, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 1, 8], 'MQ': 39, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=136685, REF=C, ALT=[A])\n",
      "8\n",
      "[0, 0, 0, 5]\n",
      "{'DP': 8, 'VDB': 0.0513395, 'SGB': -0.590765, 'MQ0F': 0.375, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 0, 5], 'MQ': 21, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=136713, REF=C, ALT=[T])\n",
      "8\n",
      "[0, 0, 1, 7]\n",
      "{'DP': 8, 'VDB': 0.00433074, 'SGB': -0.651104, 'MQSB': 1.0, 'MQ0F': 0.125, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 1, 7], 'MQ': 32, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=136761, REF=G, ALT=[A])\n",
      "11\n",
      "[0, 0, 8, 1]\n",
      "{'DP': 11, 'VDB': 0.0037412, 'SGB': -0.662043, 'MQSB': 1.0, 'MQ0F': 0.0909091, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 8, 1], 'MQ': 26, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=136771, REF=C, ALT=[A])\n",
      "6\n",
      "[0, 0, 5, 1]\n",
      "{'DP': 6, 'VDB': 0.0476592, 'SGB': -0.616816, 'MQSB': 1.0, 'MQ0F': 0.166667, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 5, 1], 'MQ': 15, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=136795, REF=T, ALT=[C])\n",
      "1\n",
      "[0, 0, 0, 1]\n",
      "{'DP': 1, 'SGB': -0.379885, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 0, 1], 'MQ': 40, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=136815, REF=T, ALT=[C])\n",
      "1\n",
      "[0, 0, 1, 0]\n",
      "{'DP': 1, 'SGB': -0.379885, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 1, 0], 'MQ': 40, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=136836, REF=C, ALT=[A])\n",
      "5\n",
      "[0, 0, 3, 2]\n",
      "{'DP': 5, 'VDB': 0.114666, 'SGB': -0.590765, 'MQSB': 0.333333, 'MQ0F': 0.2, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 3, 2], 'MQ': 34, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=136878, REF=G, ALT=[A])\n",
      "10\n",
      "[0, 0, 3, 2]\n",
      "{'DP': 10, 'VDB': 0.810473, 'SGB': -0.590765, 'MQSB': 0.666667, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 3, 2], 'MQ': 16, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=136884, REF=T, ALT=[A, C])\n",
      "14\n",
      "[0, 0, 6, 7]\n",
      "{'DP': 14, 'VDB': 2.8085e-05, 'SGB': -0.683931, 'MQSB': 0.777113, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 6, 7], 'MQ': 24, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=136933, REF=A, ALT=[T])\n",
      "16\n",
      "[1, 0, 8, 4]\n",
      "{'DP': 16, 'VDB': 0.259328, 'SGB': -0.680642, 'RPB': 1.0, 'MQB': 1.0, 'MQSB': 0.558035, 'BQB': 1.0, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [1, 0, 8, 4], 'MQ': 35, 'FQ': -999.0, 'PV4': [1.0, 1.0, 0.193646, 1.0]}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=137006, REF=C, ALT=[T, A])\n",
      "9\n",
      "[0, 0, 4, 5]\n",
      "{'DP': 9, 'VDB': 0.00127738, 'SGB': -0.662043, 'MQSB': 0.974597, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 4, 5], 'MQ': 23, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=137010, REF=G, ALT=[A])\n",
      "8\n",
      "[0, 0, 2, 5]\n",
      "{'DP': 8, 'VDB': 0.00165115, 'SGB': -0.636426, 'MQSB': 0.6, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 2, 5], 'MQ': 18, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=137049, REF=G, ALT=[T])\n",
      "3\n",
      "[0, 0, 2, 1]\n",
      "{'DP': 3, 'VDB': 0.0860477, 'SGB': -0.511536, 'MQSB': 1.0, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 2, 1], 'MQ': 27, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=137157, REF=C, ALT=[T])\n",
      "7\n",
      "[0, 0, 4, 2]\n",
      "{'DP': 7, 'VDB': 0.00595258, 'SGB': -0.616816, 'MQSB': 0.25, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 4, 2], 'MQ': 32, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=137193, REF=G, ALT=[T])\n",
      "28\n",
      "[0, 0, 6, 20]\n",
      "{'DP': 28, 'VDB': 0.000921754, 'SGB': -0.692976, 'MQSB': 0.210682, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 6, 20], 'MQ': 39, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=137233, REF=C, ALT=[T])\n",
      "2\n",
      "[0, 0, 1, 1]\n",
      "{'DP': 2, 'VDB': 0.38, 'SGB': -0.453602, 'MQSB': 1.0, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 1, 1], 'MQ': 33, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=137250, REF=T, ALT=[C])\n",
      "7\n",
      "[0, 0, 5, 1]\n",
      "{'DP': 7, 'VDB': 0.19611, 'SGB': -0.616816, 'MQSB': 1.0, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 5, 1], 'MQ': 41, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=137313, REF=T, ALT=[C])\n",
      "19\n",
      "[0, 0, 11, 7]\n",
      "{'DP': 19, 'VDB': 0.911675, 'SGB': -0.691153, 'MQSB': 0.554013, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 11, 7], 'MQ': 30, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=137349, REF=A, ALT=[G])\n",
      "21\n",
      "[0, 0, 15, 2]\n",
      "{'DP': 21, 'VDB': 0.000444517, 'SGB': -0.690438, 'MQSB': 0.633333, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 15, 2], 'MQ': 25, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=137355, REF=A, ALT=[C])\n",
      "10\n",
      "[0, 0, 10, 0]\n",
      "{'DP': 10, 'VDB': 0.00011539, 'SGB': -0.670168, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 10, 0], 'MQ': 26, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=137371, REF=G, ALT=[A])\n",
      "3\n",
      "[0, 0, 0, 2]\n",
      "{'DP': 3, 'VDB': 0.06, 'SGB': -0.453602, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 0, 2], 'MQ': 24, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=137376, REF=C, ALT=[T])\n",
      "3\n",
      "[0, 0, 1, 2]\n",
      "{'DP': 3, 'VDB': 0.0983484, 'SGB': -0.511536, 'MQSB': 1.0, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 1, 2], 'MQ': 23, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=137425, REF=G, ALT=[A])\n",
      "5\n",
      "[0, 0, 2, 1]\n",
      "{'DP': 5, 'VDB': 0.0433485, 'SGB': -0.511536, 'MQSB': 1.0, 'MQ0F': 0.2, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 2, 1], 'MQ': 27, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=137427, REF=T, ALT=[A])\n",
      "5\n",
      "[0, 0, 2, 1]\n",
      "{'DP': 5, 'VDB': 0.0349627, 'SGB': -0.511536, 'MQSB': 1.0, 'MQ0F': 0.2, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 2, 1], 'MQ': 27, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=137552, REF=T, ALT=[C])\n",
      "2\n",
      "[0, 0, 2, 0]\n",
      "{'DP': 2, 'VDB': 0.28, 'SGB': -0.453602, 'MQ0F': 0.5, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 2, 0], 'MQ': 28, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=137731, REF=C, ALT=[A])\n",
      "17\n",
      "[1, 0, 15, 1]\n",
      "{'DP': 17, 'VDB': 0.42633, 'SGB': -0.689466, 'RPB': 1.0, 'MQB': 1.0, 'MQSB': 1.0, 'BQB': 1.0, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [1, 0, 15, 1], 'MQ': 38, 'FQ': -999.0, 'PV4': [1.0, 1.0, 0.383367, 1.0]}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=137778, REF=G, ALT=[A])\n",
      "12\n",
      "[1, 0, 8, 1]\n",
      "{'DP': 12, 'VDB': 0.00175574, 'SGB': -0.662043, 'RPB': 1.0, 'MQB': 1.0, 'MQSB': 1.0, 'BQB': 1.0, 'MQ0F': 0.0833333, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [1, 0, 8, 1], 'MQ': 32, 'FQ': -999.0, 'PV4': [1.0, 1.0, 0.222554, 0.461228]}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=137784, REF=A, ALT=[G])\n",
      "7\n",
      "[0, 0, 3, 0]\n",
      "{'DP': 7, 'VDB': 0.17915, 'SGB': -0.511536, 'MQ0F': 0.142857, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 3, 0], 'MQ': 33, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=137877, REF=C, ALT=[T])\n",
      "5\n",
      "[0, 0, 1, 4]\n",
      "{'DP': 5, 'VDB': 0.0148128, 'SGB': -0.590765, 'MQSB': 1.0, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 1, 4], 'MQ': 37, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=137885, REF=A, ALT=[G])\n",
      "11\n",
      "[0, 0, 3, 6]\n",
      "{'DP': 11, 'VDB': 0.00129813, 'SGB': -0.662043, 'MQSB': 0.231146, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 3, 6], 'MQ': 37, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=137931, REF=G, ALT=[A])\n",
      "10\n",
      "[0, 0, 6, 4]\n",
      "{'DP': 10, 'VDB': 0.560195, 'SGB': -0.670168, 'MQSB': 0.167959, 'MQ0F': 0.1, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 6, 4], 'MQ': 28, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=137935, REF=C, ALT=[T, G])\n",
      "16\n",
      "[0, 0, 7, 7]\n",
      "{'DP': 16, 'VDB': 0.993788, 'SGB': -0.686358, 'MQSB': 0.194346, 'MQ0F': 0.0625, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 7, 7], 'MQ': 32, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=137964, REF=T, ALT=[C])\n",
      "27\n",
      "[0, 0, 6, 20]\n",
      "{'DP': 27, 'VDB': 1.05423e-05, 'SGB': -0.692976, 'MQSB': 0.874768, 'MQ0F': 0.037037, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 6, 20], 'MQ': 20, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=137967, REF=C, ALT=[T])\n",
      "34\n",
      "[0, 0, 8, 21]\n",
      "{'DP': 34, 'VDB': 0.000164847, 'SGB': -0.693079, 'MQSB': 0.995249, 'MQ0F': 0.0294118, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 8, 21], 'MQ': 22, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=137997, REF=C, ALT=[T, A])\n",
      "16\n",
      "[0, 0, 6, 9]\n",
      "{'DP': 16, 'VDB': 0.00323625, 'SGB': -0.688148, 'MQSB': 0.534333, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 6, 9], 'MQ': 26, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=138088, REF=C, ALT=[A, T])\n",
      "22\n",
      "[0, 0, 2, 20]\n",
      "{'DP': 22, 'VDB': 0.00146879, 'SGB': -0.692562, 'MQSB': 0.775, 'MQ0F': 0.181818, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 2, 20], 'MQ': 24, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=140522, REF=T, ALT=[C])\n",
      "26\n",
      "[0, 0, 5, 20]\n",
      "{'DP': 26, 'VDB': 0.371351, 'SGB': -0.692914, 'MQSB': 0.829508, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 5, 20], 'MQ': 38, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=140657, REF=T, ALT=[C])\n",
      "14\n",
      "[0, 0, 4, 10]\n",
      "{'DP': 14, 'VDB': 0.00268477, 'SGB': -0.686358, 'MQSB': 0.960789, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 4, 10], 'MQ': 35, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=140669, REF=T, ALT=[C])\n",
      "5\n",
      "[0, 0, 2, 1]\n",
      "{'DP': 5, 'VDB': 0.121629, 'SGB': -0.511536, 'MQSB': 1.0, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 2, 1], 'MQ': 36, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=140855, REF=G, ALT=[A, T])\n",
      "23\n",
      "[1, 0, 11, 4]\n",
      "{'DP': 23, 'VDB': 0.010128, 'SGB': -0.688148, 'RPB': 1.0, 'MQB': 1.0, 'MQSB': 0.983592, 'BQB': 1.0, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [1, 0, 11, 4], 'MQ': 39, 'FQ': -999.0, 'PV4': [1.0, 1.0, 0.309469, 1.0]}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=140972, REF=G, ALT=[A])\n",
      "27\n",
      "[0, 0, 11, 15]\n",
      "{'DP': 27, 'VDB': 0.249453, 'SGB': -0.692976, 'MQSB': 0.115917, 'MQ0F': 0.037037, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 11, 15], 'MQ': 35, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=141089, REF=A, ALT=[G])\n",
      "23\n",
      "[0, 0, 17, 6]\n",
      "{'DP': 23, 'VDB': 0.566319, 'SGB': -0.692717, 'MQSB': 0.432208, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 17, 6], 'MQ': 34, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=141116, REF=A, ALT=[G])\n",
      "33\n",
      "[0, 0, 15, 14]\n",
      "{'DP': 33, 'VDB': 0.64279, 'SGB': -0.693079, 'MQSB': 0.525288, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 15, 14], 'MQ': 34, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=141542, REF=C, ALT=[T])\n",
      "16\n",
      "[0, 0, 2, 14]\n",
      "{'DP': 16, 'VDB': 0.54149, 'SGB': -0.689466, 'MQSB': 0.857143, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 2, 14], 'MQ': 41, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=141665, REF=T, ALT=[C, G])\n",
      "30\n",
      "[0, 0, 8, 18]\n",
      "{'DP': 30, 'VDB': 8.80668e-05, 'SGB': -0.692976, 'MQSB': 0.954391, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 8, 18], 'MQ': 41, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=141755, REF=A, ALT=[G])\n",
      "13\n",
      "[0, 0, 7, 4]\n",
      "{'DP': 13, 'VDB': 0.214515, 'SGB': -0.676189, 'MQSB': 0.562708, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 7, 4], 'MQ': 37, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=144942, REF=C, ALT=[T, A, G])\n",
      "32\n",
      "[0, 0, 6, 21]\n",
      "{'DP': 32, 'VDB': 0.400081, 'SGB': -0.693021, 'MQSB': 0.276336, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 6, 21], 'MQ': 39, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=145147, REF=G, ALT=[C])\n",
      "9\n",
      "[0, 0, 4, 4]\n",
      "{'DP': 9, 'VDB': 0.327269, 'SGB': -0.651104, 'MQSB': 0.248092, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 4, 4], 'MQ': 36, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=145173, REF=A, ALT=[G])\n",
      "17\n",
      "[0, 0, 3, 11]\n",
      "{'DP': 17, 'VDB': 0.392815, 'SGB': -0.686358, 'MQSB': 0.12893, 'MQ0F': 0.0, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 3, 11], 'MQ': 36, 'FQ': -999.0}\n",
      "--------------------\n",
      "Record(CHROM=AF409138.1, POS=150349, REF=G, ALT=[A])\n",
      "29\n",
      "[0, 0, 10, 17]\n",
      "{'DP': 29, 'VDB': 0.00241937, 'SGB': -0.693021, 'MQSB': 0.709516, 'MQ0F': 0.206897, 'AF1': 1.0, 'AC1': 1.0, 'DP4': [0, 0, 10, 17], 'MQ': 1, 'FQ': -999.0}\n",
      "--------------------\n",
      "538\n"
     ]
    }
   ],
   "source": [
    "vcf_reader = vcf.Reader(open('./samtools_out/saratov_vars.vcf', 'r'))\n",
    "counter = 0\n",
    "for record in vcf_reader:\n",
    "    print(record)\n",
    "    print(record.INFO[\"DP\"])\n",
    "    print(record.INFO[\"DP4\"])\n",
    "    print(record.INFO)\n",
    "    print(\"--------------------\")\n",
    "    counter += 1\n",
    "\n",
    "print(counter)\n",
    "    "
   ]
  },
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": [
    "#### total number of SNPs in vcf :"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 14,
   "metadata": {},
   "outputs": [
    {
     "name": "stdout",
     "output_type": "stream",
     "text": [
      "total SNPs :  520\n"
     ]
    }
   ],
   "source": [
    "vcf_reader = vcf.Reader(open('./samtools_out/saratov_vars.vcf', 'r'))\n",
    "counter = 0\n",
    "for record in vcf_reader:\n",
    "    if \"INDEL\" not in record.INFO.keys():\n",
    "        counter += 1\n",
    "\n",
    "print(\"total SNPs : \", counter)"
   ]
  },
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": [
    "\n",
    "####  DP4 contains 4 values:\n",
    "1. forward ref alleles\n",
    "2. reverse ref\n",
    "3. forward non-ref \n",
    "4. reverse non-ref alleles\n",
    "\n",
    "Sum can be smaller than DP (The number of reads covering or bridging POS)  because low-quality bases are not counted.\n",
    "\n",
    "#### SNPs with coverage more than 10:"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 15,
   "metadata": {},
   "outputs": [
    {
     "name": "stdout",
     "output_type": "stream",
     "text": [
      "SNP passing filter:  388\n"
     ]
    }
   ],
   "source": [
    "vcf_reader = vcf.Reader(open('./samtools_out/saratov_vars.vcf', 'r'))\n",
    "counter = 0\n",
    "for record in vcf_reader:\n",
    "    if \"INDEL\" not in record.INFO.keys():\n",
    "        if record.INFO[\"DP4\"][2] + record.INFO[\"DP4\"][3] >= 10:\n",
    "            counter += 1\n",
    "print(\"SNP passing filter: \", counter)"
   ]
  },
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": [
    "#### 8. consensus calling\n",
    "- to create consensus we apply variants to the reference\n",
    "\n",
    "#### NOTE : if we want to get consensus, we'll have to redo the calling step, as bcftools can apply only files compressed with bgzip to the reference. If we want just to get vcf file, we don't need this step \n",
    "\n",
    "the command `-Oz -o` means ouput in bzip format \n",
    "- we need this compressed format to get consensus (otherwise it doesn't work)\n",
    "- then we must index compressed `vcf` file, we need it derive consensus as well"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 8,
   "metadata": {},
   "outputs": [],
   "source": [
    "%%bash\n",
    "bcftools call \\\n",
    "--consensus-caller \\\n",
    "--variants-only \\\n",
    "--skip-variants indels \\\n",
    "--ploidy-file ./samtools_out/ploid.txt \\\n",
    "./samtools_out/saratov_vars.bcf \\\n",
    "-Oz -o ./samtools_out/saratov_vars.vcf.gz \\"
   ]
  },
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": [
    "#### we must index the `lsd_2_vars.vcf.gz`"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 9,
   "metadata": {},
   "outputs": [],
   "source": [
    "%%bash\n",
    "bcftools tabix -p vcf ./samtools_out/saratov_vars.vcf.gz"
   ]
  },
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": [
    "#### creating consensus"
   ]
  },
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": [
    "Create consensus sequence by applying VCF variants to a reference fasta file.\n",
    "\n",
    "- reference sequence\n",
    "- `bcf` file with variants calls\n",
    "- indexed `vcf` file\n",
    "\n",
    "but:\n",
    "- we didn't call indels, so we don't need to normalize and filter indels\n",
    "\n",
    "see : http://samtools.github.io/bcftools/howtos/consensus-sequence.html"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 5,
   "metadata": {},
   "outputs": [
    {
     "name": "stdout",
     "output_type": "stream",
     "text": [
      "total 446644\n",
      "-rwxrwxrwx 1 root root  33407745 Feb 15 10:45 1_trim_crop_and_trail_32_1P.fastq\n",
      "-rwxrwxrwx 1 root root   1921926 Feb 15 10:45 1_trim_crop_and_trail_32_1U.fastq\n",
      "-rwxrwxrwx 1 root root  33002253 Feb 15 10:45 1_trim_crop_and_trail_32_2P.fastq\n",
      "-rwxrwxrwx 1 root root    186221 Feb 15 10:45 1_trim_crop_and_trail_32_2U.fastq\n",
      "-rwxrwxrwx 1 root root  26364751 Feb 18 10:44 2_trim_crop_and_trail_32_2P.fastq\n",
      "-rwxrwxrwx 1 root root 133352346 Jun 25  2018 SUD-Saratov_S1_L001_R1_001.fastq\n",
      "-rwxrwxrwx 1 root root 138569286 Jun 25  2018 SUD-Saratov_S1_L001_R2_001.fastq\n",
      "-rwxrwxrwx 1 root root  88870486 Feb 18 12:59 input_data.rar\n",
      "-rwxrwxrwx 1 root root   1363903 Feb 14 16:47 merged_document.aln\n",
      "-rwxrwxrwx 1 root root    152749 Jun 25  2018 ref_AF409138.1.fasta\n",
      "-rwxrwxrwx 1 root root        27 Feb 14 17:40 ref_AF409138.1.fasta.fai\n",
      "-rwxrwxrwx 1 root root    150532 Feb 14 16:43 saratov_map_on_AF_consensus.fa\n"
     ]
    }
   ],
   "source": [
    "%%bash\n",
    "ls -l ./input_data"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 10,
   "metadata": {},
   "outputs": [
    {
     "name": "stderr",
     "output_type": "stream",
     "text": [
      "Note: the --sample option not given, applying all records\n"
     ]
    }
   ],
   "source": [
    "%%bash\n",
    "cat ./input_data/ref_AF409138.1.fasta | \\\n",
    "bcftools consensus \\\n",
    "./samtools_out/saratov_vars.vcf.gz \\\n",
    "> ./samtools_out/consensus_seq_.fasta"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": null,
   "metadata": {},
   "outputs": [],
   "source": []
  },
  {
   "cell_type": "code",
   "execution_count": null,
   "metadata": {},
   "outputs": [],
   "source": []
  },
  {
   "cell_type": "code",
   "execution_count": null,
   "metadata": {},
   "outputs": [],
   "source": []
  },
  {
   "cell_type": "code",
   "execution_count": null,
   "metadata": {},
   "outputs": [],
   "source": []
  },
  {
   "cell_type": "code",
   "execution_count": null,
   "metadata": {},
   "outputs": [],
   "source": []
  }
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