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Any idea about the multiple sample looms to integrated by loompy.combine function? #10

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honghh2018 opened this issue Jul 8, 2021 · 1 comment
Open

Any idea about the multiple sample looms to integrated by loompy.combine function? #10

honghh2018 opened this issue Jul 8, 2021 · 1 comment

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@honghh2018
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Hi all,
Thanks to develop the great pipeline to manipulate the RNA velocity between seurat and anndata object.
I wonder that the vignette you post about the merge is one by one to filter and then taking the sample_one.concatenate function to merge the loom data. in my case, i used to the loompy.combine function to aggregate 20 samples' loom and then filter the barcode identical with seurat's barcode. after that, using the seurat object convert to anndata to analyize the velocity.
Is that right ? and can the velocity apply to the multiple samples?
Any advice would be appreciated.
Best,
Hanhuihong
@honghh2018 honghh2018 changed the title how about the multiple sample looms to integrated by loompy.combine function? Any idea about the multiple sample looms to integrated by loompy.combine function? Jul 8, 2021
@honghh2018
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Had anyone hear this?

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@honghh2018