Database and bowtie issues

[WebBach]

Hi, I'm trying to use metaphlan 3.0 to calculate the microbial community abundance of my samples. But I get an error that I can't solve. I did an iteration for loop over each sample just to visualize better the dynamics of the run. But the idea is to run them in parallel.
The code is based on your guidance:

metaphlan metagenome_1.fastq,metagenome_2.fastq --bowtie2out metagenome.bowtie2.bz2 --bowtie2db --nproc 5 --input_type fastq -o profiled_metagenome.txt

But I get this repeated error in each sample:

My question are:

1. why does it try o download the database every time that runs the code if I already specify the database folder?
2. how can I solve this bowtie error?

Thank you

[fanninpm]

Try typing the --bowtie2db argument without the trailing slash.

[WebBach]

Hey, thanks for the suggestion but I still get exactly the same error :(

[julianeweller]

I'm having the same issue when installing the tool:
metaphlan --install --bowtie2db [folderpath]

Any suggestions on how to solve this? Thanks!

[julianeweller]

Installing libtbb2 solved the problem for me:
sudo apt-get install libtbb2

[fbeghini]

Please use the BioBakery forum for asking for help. See #123 .