AssertionError while trying to run Phy2 post-sorting

[KyleWei-UChi]

Hi,

I've been using kilosort 2 for sorting and phy2 for visualization/selection of units post-sorting for a while now. It usually works smoothly until today when I got a large trunk of trace back errors and a "QWidget: Must construct a QApplication before a QWidget" message.

I have limited experience with phy. From reading the errors I guess that something absurd was happening with spike templates, probably because I only got 2 sorted units form kilosort from this session. However, I used to have data with only a few units (like 2 or 3, especially when I was testing the system), and phy worked fine with those datasets.

Here I attached the error messages below - any suggestions/recommendations about solving the problem would be much appreciated!

(phy2) C:\Users\Maunsell Lab>cd C:\Users\Maunsell Lab\Documents\Lai\Meetz_250117\kilosort

(phy2) C:\Users\Maunsell Lab\Documents\Lai\Meetz_250117\kilosort>phy template-gui params.py
23:09:07.833 [W] model:667 Skipping spike waveforms that do not exist, they will be extracted on the fly from the raw data as needed.
23:09:07.836 [E] init:62 An error has occurred (AssertionError):
Traceback (most recent call last):
File "", line 198, in _run_module_as_main
File "", line 88, in run_code
File "C:\ProgramData\anaconda3\envs\phy2\Scripts\phy.exe_main.py", line 7, in
sys.exit(phycli())
^^^^^^^^
File "C:\ProgramData\anaconda3\envs\phy2\Lib\site-packages\click\core.py", line 1157, in call
return self.main(*args, **kwargs)
^^^^^^^^^^^^^^^^^^^^^^^^^^
File "C:\ProgramData\anaconda3\envs\phy2\Lib\site-packages\click\core.py", line 1078, in main
rv = self.invoke(ctx)
^^^^^^^^^^^^^^^^
File "C:\ProgramData\anaconda3\envs\phy2\Lib\site-packages\click\core.py", line 1688, in invoke
return _process_result(sub_ctx.command.invoke(sub_ctx))
^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
File "C:\ProgramData\anaconda3\envs\phy2\Lib\site-packages\click\core.py", line 1434, in invoke
return ctx.invoke(self.callback, **ctx.params)
^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
File "C:\ProgramData\anaconda3\envs\phy2\Lib\site-packages\click\core.py", line 783, in invoke
return _callback(*args, **kwargs)
^^^^^^^^^^^^^^^^^^^^^^^^^^^
File "C:\ProgramData\anaconda3\envs\phy2\Lib\site-packages\click\decorators.py", line 33, in new_func
return f(get_current_context(), *args, **kwargs)
^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
File "C:\ProgramData\anaconda3\envs\phy2\Lib\site-packages\phy\apps_init.py", line 159, in cli_template_gui
template_gui(params_path, **kwargs)
File "C:\ProgramData\anaconda3\envs\phy2\Lib\site-packages\phy\apps\template\gui.py", line 209, in template_gui
model = load_model(params_path)
^^^^^^^^^^^^^^^^^^^^^^^
File "C:\ProgramData\anaconda3\envs\phy2\Lib\site-packages\phylib\io\model.py", line 1440, in load_model
return TemplateModel(**get_template_params(params_path))
^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
File "C:\ProgramData\anaconda3\envs\phy2\Lib\site-packages\phylib\io\model.py", line 339, in init
self._load_data()
File "C:\ProgramData\anaconda3\envs\phy2\Lib\site-packages\phylib\io\model.py", line 446, in _load_data
assert self.similar_templates.shape == (self.n_templates, self.n_templates)
^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
AssertionError

QWidget: Must construct a QApplication before a QWidget