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Okay,
I'm just going to say it and risk international humiliation but:
SRR2584866_final_variants.vcf is missing the "results" column data in the lesson. This makes the explanation of VCF file format more difficult.
Also, There are only GT:PL metrics presented in SRR2584866_final_variants.vcf. There's no "GQ" in the entire output, and the INFO column does have "DP" (and "DP4") but no "AD". The lack of GQ, and AD, combined with "DP" in a different column, makes explaining VCF format difficult.
Here's the complete file:
#CHROM POS ID REF ALT QUAL FILTER INFO FORMAT results/bam/SRR2584866.aligned.sorted.bam
CP000819.1 1521 . C T 207 . DP=9;VDB=0.993024;SGB=-0.662043;MQSB=0.974597;MQ0F=0;AC=1;AN=1;DP4=0,0,4,5;MQ=60 GT:PL 1:237,0
CP000819.1 1612 . A G 225 . DP=13;VDB=0.52194;SGB=-0.676189;MQSB=0.950952;MQ0F=0;AC=1;AN=1;DP4=0,0,6,5;MQ=60 GT:PL 1:255,0
CP000819.1 9092 . A G 225 . DP=14;VDB=0.717543;SGB=-0.670168;MQSB=0.916482;MQ0F=0;AC=1;AN=1;DP4=0,0,7,3;MQ=60 GT:PL 1:255,0
CP000819.1 9972 . T G 214 . DP=10;VDB=0.022095;SGB=-0.670168;MQSB=1;MQ0F=0;AC=1;AN=1;DP4=0,0,2,8;MQ=60 GT:PL 1:244,0
CP000819.1 10563 . G A 225 . DP=11;VDB=0.958658;SGB=-0.670168;MQSB=0.952347;MQ0F=0;AC=1;AN=1;DP4=0,0,5,5;MQ=60 GT:PL 1:255,0
CP000819.1 22257 . C T 127 . DP=5;VDB=0.0765947;SGB=-0.590765;MQSB=1;MQ0F=0;AC=1;AN=1;DP4=0,0,2,3;MQ=60 GT:PL 1:157,0
CP000819.1 38971 . A G 225 . DP=14;VDB=0.872139;SGB=-0.680642;MQSB=1;MQ0F=0;AC=1;AN=1;DP4=0,0,4,8;MQ=60 GT:PL 1:255,0
CP000819.1 42306 . A G 225 . DP=15;VDB=0.969686;SGB=-0.686358;MQSB=1;MQ0F=0;AC=1;AN=1;DP4=0,0,5,9;MQ=60 GT:PL 1:255,0
CP000819.1 45277 . A G 225 . DP=15;VDB=0.470998;SGB=-0.680642;MQSB=0.95494;MQ0F=0;AC=1;AN=1;DP4=0,0,7,5;MQ=60 GT:PL 1:255,0
CP000819.1 56613 . C G 183 . DP=12;VDB=0.879703;SGB=-0.676189;MQSB=1;MQ0F=0;AC=1;AN=1;DP4=0,0,8,3;MQ=60 GT:PL 1:213,0
CP000819.1 62118 . A G 225 . DP=19;VDB=0.414981;SGB=-0.691153;MQSB=0.906029;MQ0F=0;AC=1;AN=1;DP4=0,0,8,10;MQ=59 GT:PL 1:255,0
CP000819.1 64042 . G A 225 . DP=18;VDB=0.451328;SGB=-0.689466;MQSB=1;MQ0F=0;AC=1;AN=1;DP4=0,0,7,9;MQ=60 GT:PL 1:255,0
The text was updated successfully, but these errors were encountered:
Okay,
I'm just going to say it and risk international humiliation but:
SRR2584866_final_variants.vcf is missing the "results" column data in the lesson. This makes the explanation of VCF file format more difficult.
Also, There are only GT:PL metrics presented in SRR2584866_final_variants.vcf. There's no "GQ" in the entire output, and the INFO column does have "DP" (and "DP4") but no "AD". The lack of GQ, and AD, combined with "DP" in a different column, makes explaining VCF format difficult.
Here's the complete file:
#CHROM POS ID REF ALT QUAL FILTER INFO FORMAT results/bam/SRR2584866.aligned.sorted.bam
CP000819.1 1521 . C T 207 . DP=9;VDB=0.993024;SGB=-0.662043;MQSB=0.974597;MQ0F=0;AC=1;AN=1;DP4=0,0,4,5;MQ=60 GT:PL 1:237,0
CP000819.1 1612 . A G 225 . DP=13;VDB=0.52194;SGB=-0.676189;MQSB=0.950952;MQ0F=0;AC=1;AN=1;DP4=0,0,6,5;MQ=60 GT:PL 1:255,0
CP000819.1 9092 . A G 225 . DP=14;VDB=0.717543;SGB=-0.670168;MQSB=0.916482;MQ0F=0;AC=1;AN=1;DP4=0,0,7,3;MQ=60 GT:PL 1:255,0
CP000819.1 9972 . T G 214 . DP=10;VDB=0.022095;SGB=-0.670168;MQSB=1;MQ0F=0;AC=1;AN=1;DP4=0,0,2,8;MQ=60 GT:PL 1:244,0
CP000819.1 10563 . G A 225 . DP=11;VDB=0.958658;SGB=-0.670168;MQSB=0.952347;MQ0F=0;AC=1;AN=1;DP4=0,0,5,5;MQ=60 GT:PL 1:255,0
CP000819.1 22257 . C T 127 . DP=5;VDB=0.0765947;SGB=-0.590765;MQSB=1;MQ0F=0;AC=1;AN=1;DP4=0,0,2,3;MQ=60 GT:PL 1:157,0
CP000819.1 38971 . A G 225 . DP=14;VDB=0.872139;SGB=-0.680642;MQSB=1;MQ0F=0;AC=1;AN=1;DP4=0,0,4,8;MQ=60 GT:PL 1:255,0
CP000819.1 42306 . A G 225 . DP=15;VDB=0.969686;SGB=-0.686358;MQSB=1;MQ0F=0;AC=1;AN=1;DP4=0,0,5,9;MQ=60 GT:PL 1:255,0
CP000819.1 45277 . A G 225 . DP=15;VDB=0.470998;SGB=-0.680642;MQSB=0.95494;MQ0F=0;AC=1;AN=1;DP4=0,0,7,5;MQ=60 GT:PL 1:255,0
CP000819.1 56613 . C G 183 . DP=12;VDB=0.879703;SGB=-0.676189;MQSB=1;MQ0F=0;AC=1;AN=1;DP4=0,0,8,3;MQ=60 GT:PL 1:213,0
CP000819.1 62118 . A G 225 . DP=19;VDB=0.414981;SGB=-0.691153;MQSB=0.906029;MQ0F=0;AC=1;AN=1;DP4=0,0,8,10;MQ=59 GT:PL 1:255,0
CP000819.1 64042 . G A 225 . DP=18;VDB=0.451328;SGB=-0.689466;MQSB=1;MQ0F=0;AC=1;AN=1;DP4=0,0,7,9;MQ=60 GT:PL 1:255,0
The text was updated successfully, but these errors were encountered: