You signed in with another tab or window. Reload to refresh your session.You signed out in another tab or window. Reload to refresh your session.You switched accounts on another tab or window. Reload to refresh your session.Dismiss alert
Hello!
I was able to successfully run the FRASER analysis with 11 local bam files. To increase the sample size, I added the data in GTEx. The operation went smoothly at first. But in running rule AberrantSplicing_pipeline_Counting_03_filter_expression_FraseR_R:
There was an error like this:
Loading provided [counts]
Wed Apr 12 09:57:14 2023: Merging data ...
Wed Apr 12 09:57:17 2023: Calculate the PSI 5 and 3 values ...
Wed Apr 12 09:57:26 2023: Calculate the PSI site values ...
Error: subscript is a logical vector with out-of-bounds TRUE values
In addition: Warning message:
In .merge_two_Seqinfo_objects(x, y) :
The 2 combined objects have no sequence levels in common. (Use
suppressWarnings() to suppress this warning.)
Execution halted
Is it a merger problem? My bam file is compared by STAR software and fa in STAR database. I am sure it is hg19.
I uploaded my relevant files
How can I solve this problem? Increasing the sample size by external counting is important to me
I would really appreciate it if you could answer my questions.
Hi! Weird, DROP should be able to recognize different chr styles. Can you double check if indeed your local BAM files have a different chr style than the external junction count matrices?
Hello!
I was able to successfully run the FRASER analysis with 11 local bam files. To increase the sample size, I added the data in GTEx. The operation went smoothly at first. But in running rule AberrantSplicing_pipeline_Counting_03_filter_expression_FraseR_R:
There was an error like this:
Loading provided [counts]
Wed Apr 12 09:57:14 2023: Merging data ...
Wed Apr 12 09:57:17 2023: Calculate the PSI 5 and 3 values ...
Wed Apr 12 09:57:26 2023: Calculate the PSI site values ...
Error: subscript is a logical vector with out-of-bounds TRUE values
In addition: Warning message:
In .merge_two_Seqinfo_objects(x, y) :
The 2 combined objects have no sequence levels in common. (Use
suppressWarnings() to suppress this warning.)
Execution halted
Is it a merger problem? My bam file is compared by STAR software and fa in STAR database. I am sure it is hg19.
I uploaded my relevant files
How can I solve this problem? Increasing the sample size by external counting is important to me
I would really appreciate it if you could answer my questions.
sample_annotation_put.txt
nohup.log
config_put.txt
The text was updated successfully, but these errors were encountered: