diff --git a/README.md b/README.md
index 5c3d05f..4d59a09 100644
--- a/README.md
+++ b/README.md
@@ -57,7 +57,8 @@ pip3 install -r requirements.txt
 
 
 ## Usage
-
+> [!NOTE]
+> SVEN was trained and evaluated based on reference genome <b>GRCh38</b>.
 #### Predict gene expression level based on TSS of genes
 
 ```bash