diff --git a/methods.py b/methods.py new file mode 100644 index 0000000..9e37d36 --- /dev/null +++ b/methods.py @@ -0,0 +1,68 @@ +import numpy as np +import pandas as pd +import matplotlib.pyplot as plt +import seaborn as sns + +# ask user to input the file path +def get_file_path(): + file_path = input("Please enter the file path: ") + return file_path + +# read the file +def read_file(file_path): + try: + data = pd.read_csv(file_path) + return data + except FileNotFoundError: + print("File not found. Please enter a valid file path.") + return None + except Exception as e: + print("An error occurred. Please try again.") + return None + +# display the columns of the data +def display_columns(data): + print("The columns in the data are: ") + for column in data.columns: + print(column) + + +# pick the graph type to plot: volcano plot, MA plot, PCA plot, heatmap +def pick_graph_type(): + print("Please pick the graph type you would like to plot: ") + print("1. Volcano plot") + print("2. MA plot") + print("3. PCA plot") + print("4. Heatmap") + graph_type = input("Enter the number of the graph type you would like to plot: ") + return graph_type + +# volcano plot +def volcano_plot(data): + x_axis = input("Enter the column name for X axis: ") + y_axis = input("Enter the column name for Y axis: ") + threshold = float(input("Enter the threshold value: ")) + + plt.scatter(x=data[x_axis], y=data[y_axis], s=1, label="Not significant") + + # highlight down- or up- regulated genes + down = data[(data[x_axis] <= -threshold) & (data[y_axis] >= threshold)] + up = data[(data[x_axis] >= threshold) & (data[y_axis] >= threshold)] + + plt.scatter(x=down[x_axis], y=down[y_axis], s=3, label="Down-regulated", color="blue") + plt.scatter(x=up[x_axis], y=up[y_axis], s=3, label="Up-regulated", color="red") + + plt.xlabel(x_axis) + plt.ylabel(y_axis) + plt.axvline(-threshold, color="grey", linestyle="--") + plt.axvline(threshold, color="grey", linestyle="--") + plt.axhline(threshold, color="grey", linestyle="--") + plt.legend() + plt.show() + + + +# main function + +df = pd.read_csv('test.csv') +volcano_plot(df) \ No newline at end of file diff --git a/test.csv b/test.csv new file mode 100644 index 0000000..f9499c1 --- /dev/null +++ b/test.csv @@ -0,0 +1,305 @@ +Name,Obp44a-/-_1,Obp44a-/-_2,Obp44a-/-_3,WT_1,WT_2,WT_3,7A Avg,CS Avg,FC,Log2FC,pval,minuslog10(pval) +13-HODE,6652.666667,8484.666667,4238.666667,2898,2331,2392.666667,6458.666667,2540.555556,2.54222611,1.346092352,0.034409127,1.463326342 +DG(i-14:0/0:0/13:0),2812.666667,2066,2966,1888,1489.333333,1759.333333,2614.888889,1712.222222,1.527190136,0.61087969,0.040297441,1.394722537 +L-Phosphoarginine,1187180,1643805.333,1691681.333,814283.3333,1172822,686245.3333,1507555.555,891116.8889,1.691759604,0.758524579,0.046786776,1.329876879 +LysoPE(0:0/20:0),4179.333333,3395.333333,4147.333333,2556,2978,1854.666667,3907.333333,2462.888889,1.586483804,0.665832793,0.025506115,1.59335569 +MG(22:0/0:0/0:0),54641.33333,55478.66667,55193.33333,41929.33333,34877.33333,37518,55104.44444,38108.22222,1.445998822,0.532066377,0.001202731,2.919831589 +PE(30:2),7172.666667,5867.333333,7421.333333,3868,4305.333333,3879.333333,6820.444444,4017.555555,1.697660269,0.76354778,0.005081089,2.294043181 +PE(32:1),60390.66667,59908,58551.33333,53506,48725.33333,46332,59616.66667,49521.11111,1.203863672,0.267672028,0.0097925,2.009106439 +CerPE(d14:1(4E)/19:0),2326,2342,1912.666667,1562,1463.333333,1296,2193.555556,1440.444444,1.522832459,0.606757227,0.009370126,2.028254584 +3-hydroxyoctanoyl carnitine,4454.666667,3430,4032,6262,5340.666667,5068.666667,3972.222222,5557.111111,0.714799856,-0.484388751,0.027568828,1.559581699 +Ala Val Met,1521,1364,1218.666667,1620,1716,1708.666667,1367.888889,1681.555556,0.813466367,-0.297845396,0.027587362,1.559289827 +"D-Fructose 2,6-bisphosphate",58179.33333,41331.33333,32968,84094,65458.66667,110672,44159.55556,86741.55556,0.509093424,-0.973997663,0.047546807,1.32287864 +"DG(14:0/18:3(6Z,9Z,12Z)/0:0)",9929.333333,7514,10823.33333,17076.66667,14235.33333,15474.66667,9422.222221,15595.55556,0.604160729,-0.726995683,0.008641447,2.063413523 +DG(16:1(9Z)/14:1(9Z)/0:0),9052,6014,7992.666667,15796.66667,12457.33333,13977.33333,7686.222222,14077.11111,0.546008493,-0.873004703,0.008238357,2.084159402 +"DG(16:1(9Z)/20:2(11Z,14Z)/0:0)",12230,10830,10061.33333,20492.66667,21146.66667,14995.33333,11040.44444,18878.22222,0.584824371,-0.773924661,0.018764194,1.726670091 +DG(18:1(11Z)/14:1(9Z)/0:0),18000.66667,13636,16718,24151.33333,20914,20898,16118.22222,21987.77778,0.733053717,-0.448009175,0.02539318,1.5952829 +D-Glutamine,1943217.333,1838880.667,1992750,2157962,2060070.667,2253691.333,1924949.333,2157241.333,0.892319883,-0.164367109,0.032056641,1.494081981 +Docosanoylcarnitine,6847.333333,4134.666667,5428.666667,9590.666667,9526.666667,12784.66667,5470.222222,10634,0.51440871,-0.959013024,0.017826546,1.748932808 +Glycerophosphoglycerol,11898.66667,11515.33333,14493.33333,21414,18837.33333,20660.66667,12635.77778,20304,0.622329481,-0.684249504,0.003157718,2.500626629 +Hydroxyhexanoycarnitine,1695,1097.333333,1658.666667,3460,2327.333333,2700.666667,1483.666667,2829.333333,0.52438737,-0.931295155,0.025070168,1.600842747 +L-Glutamic acid 5-phosphate,1462.666667,1044.666667,905.3333333,2788.666667,2247.333333,3172.666667,1137.555556,2736.222222,0.415739462,-1.266248399,0.007214226,2.141810237 +MG(14:0/0:0/0:0),4512.666667,3862.666667,3314,4908.666667,4973.333333,4899.333333,3896.444444,4927.111111,0.790817247,-0.33858376,0.04121127,1.384984002 +Pantetheine 4'-phosphate,2975.333333,1772,2050.666667,11606.66667,9588,7229.333333,2266,9474.666668,0.239164087,-2.063927327,0.005408829,2.26689673 +"PG(16:0/18:3(9Z,12Z,15Z))",15536,17152,17480.66667,19050,20972.66667,21152.66667,16722.88889,20391.77778,0.820079989,-0.286163461,0.015265698,1.816283341 +"PI(18:0/18:3(9Z,12Z,15Z))",9968.666667,9842,11780.66667,13118,13015.33333,12031.33333,10530.44445,12721.55555,0.827763901,-0.272708762,0.037587671,1.42495458 +"PI(18:1(11Z)/18:3(9Z,12Z,15Z))",7258,8701.333333,9248.666667,10688.66667,11420.66667,10688.66667,8402.666667,10932.66667,0.76858345,-0.379726184,0.016941578,1.771046152 +(±)-Propionylcarnitine,8481.333333,8164.666667,8778.666667,9121.333333,22259.33333,12706,8474.888889,14695.55556,0.576697414,-0.794113541,0.188163349,0.725464966 +"(5Z,8Z)-Tetradecadienoylcarnitine",2064,721.3333333,1963.333333,3048.666667,1620,3736,1582.888889,2801.555556,0.565003569,-0.823668113,0.183243391,0.736971681 +16-Methylheptadecanoic acid,240672,223504.6667,190149.3333,267356.6667,173484,194804,218108.6667,211881.5556,1.029389586,0.041789092,0.855417252,0.067821995 +2-Amino-3-oxoadipate,87388.66667,99030,54404.66667,83009.33333,95387.33333,106974.6667,80274.44445,95123.77779,0.843894621,-0.244865236,0.379645559,0.420621676 +2-Hydroxymyristoylcarnitine,3742.666667,1550,2366,5364,2730,4330.666667,2552.888889,4141.555556,0.61640822,-0.698041994,0.186716986,0.728816171 +2-O-(6-Phospho-alpha-mannosyl)-D-glycerate,67458.66667,83315.33333,224874.6667,118801.3333,88152,176892.6667,125216.2222,127948.6667,0.978644213,-0.031143633,0.963682657,0.016065957 +3-Hydroxy-9Z-octadecenoylcarnitine,1989,892,1270.666667,2690.666667,1756,3168,1383.888889,2538.222222,0.545219751,-0.875090268,0.092709274,1.032876821 +3-Hydroxy-cis-5-tetradecenoylcarnitine,2152.666667,1092.666667,1695.333333,4041.333333,2142.666667,3048,1646.888889,3077.333333,0.535167533,-0.9019375,0.085119916,1.069968815 +3-hydroxydecanoyl carnitine,2244,1713.333333,1883,3978.666667,2203.333333,3358,1946.777778,3180,0.61219427,-0.707938554,0.085696288,1.067037991 +3-hydroxydodecanoyl carnitine,2101.333333,1098,1938,4010,2238,3406.666667,1712.444444,3218.222222,0.532108825,-0.910206765,0.067841819,1.168502513 +3-hydroxyhexadecanoyl carnitine,6360.666667,2456,3450.666667,8532.666667,4541.333333,8027.333333,4089.111111,7033.777778,0.581353469,-0.782512491,0.161419633,0.792043643 +3-Methylguanine,11650.66667,8388.666667,7686.666667,11012,7154.666667,5811,9242,7992.555556,1.156326026,0.209548222,0.562317542,0.250018368 +4-(Glutamylamino) butanoate,9905,7330.666667,10136,11778.66667,7094,8749.333333,9123.888889,9207.333333,0.990937176,-0.0131345,0.961860289,0.016888005 +4-Hydroxyphenylacetylglutamic acid,1622,1561.333333,1549.333333,1668.666667,1362.666667,1728.666667,1577.555555,1586.666667,0.994257703,-0.008308261,0.940934654,0.026440536 +5-Acetylamino-6-formylamino-3-methyluracil,8067,8437.333333,13683.33333,6230,6293.333333,13645,10062.55556,8722.777778,1.153595312,0.206137207,0.683840371,0.165045264 +5'-Methylthioadenosine,6004,6200,6838,7749.333333,5770,5963.333333,6347.333333,6494.222222,0.977381604,-0.033006144,0.839192736,0.076138284 +"6-Chloro-N-(1-methylethyl)-1,3,5-triazine-2,4-diamine",153422.6667,200842,221054.6667,153935.3333,140171.3333,177857.3333,191773.1111,157321.3333,1.218989867,0.285686133,0.206399772,0.685290787 +"7,8-Dihydroneopterin",5584,2396,5282.666667,5971,3432,2822,4420.888889,4075,1.084880709,0.117536416,0.817127246,0.087710308 +8-oxo-dGDP,1535.333333,1253,1385,1268,1296,1169,1391.111111,1244.333333,1.11795696,0.160864648,0.179015333,0.74710977 +Acetylcarnitine,2918939.333,2121689.333,3355421.333,3783402,3379502.667,3576361.333,2798683.333,3579755.333,0.781808552,-0.355112729,0.108708798,0.963735305 +Acetylcholine,57134,49677.33333,71692.66667,70539.33333,52684,64764.66667,59501.33333,62662.66667,0.949549971,-0.074684169,0.723734971,0.140420442 +Adenosine 2'-phosphate,118826,32988.66667,55924,206123.3333,32680.66667,202216.6667,69246.22222,147006.8889,0.471040662,-1.086076492,0.282468712,0.54902965 +"Adenosine 3',5'-diphosphate",236246.6667,82448.66667,118547.3333,237173.3333,99749.33333,480485.3333,145747.5556,272469.3333,0.534913613,-0.902622175,0.352704286,0.452589263 +Ala His Ser,3423.333333,8762,2192.666667,1918.666667,1422.666667,1813.333333,4792.666667,1718.222222,2.789317124,1.479911967,0.202997143,0.692510074 +"alpha-D-Glucose 1,6-bisphosphate",372090,321281.3333,241900.6667,425618,395299.3333,566546,311757.3333,462487.7778,0.674087724,-0.568991743,0.081079009,1.091091568 +aminocaproic acid,11331,4444,11666.66667,14586,9153.333333,9205,9147.222222,10981.44444,0.832970769,-0.263662226,0.569582423,0.244443422 +Aminofructose 6-phosphate,12663.33333,11740,14710,12387,10792,15512.66667,13037.77778,12897.22222,1.010898126,0.015637616,0.935851414,0.028793099 +Arg Asn Val Arg,15245.33333,13623.33333,18900.66667,16752,15351.33333,22216,15923.11111,18106.44444,0.879416782,-0.185381031,0.450205166,0.346589526 +Arg Glu Gln,2053.333333,1577.333333,1974.666667,1781.333333,1571,1854.666667,1868.444444,1735.666667,1.076499584,0.106347762,0.478622543,0.32000685 +Arg Thr Gln,3744,4158,4000.666667,3304,3830,4120.666667,3967.555556,3751.555556,1.057576117,0.080761502,0.465012174,0.332535677 +Asn Arg Thr,2418,2532,2793.333333,2659,2271.333333,2233.333333,2581.111111,2387.888889,1.080917593,0.11225654,0.332966106,0.477599973 +Asp Asp Asp,49486,38600.66667,64044,38638.66667,34626,41894.66667,50710.22222,38386.44445,1.321045045,0.40167966,0.183123874,0.737255033 +Asp Ile Ser Glu,10118,7249.333333,9726,12931.33333,10787.33333,12511.33333,9031.111111,12076.66666,0.747814887,-0.419246904,0.051983386,1.284135436 +Aspartylglycosamine,6108,5296,5580,7236,5912,6398.666667,5661.333333,6515.555556,0.868894952,-0.202746326,0.133029317,0.876052639 +beta-N-Acetylglucosamine,4347,2772.666667,5728.666667,4546,2836,3257.333333,4282.777778,3546.444444,1.207625791,0.272173474,0.501119704,0.300058521 +CDP,8165.333333,2733.333333,1341,4030.666667,3088.666667,7542,4079.888889,4887.111111,0.8348263,-0.260452043,0.761492314,0.118334476 +CDP-ethanolamine,109209.3333,91926,94221.33333,83656.66667,89160,96943.33333,98452.22221,89920,1.094886813,0.130781735,0.268778202,0.570605956 +Cer(d18:0/18:1(11Z)),77644,123254,86774,129347.3333,113712,98070,95890.66667,113709.7778,0.843293062,-0.24589401,0.343592035,0.463956912 +Cer(d18:1/12:0),8965.333333,11062.66667,8898,6682.666667,10204,12193.33333,9642.000001,9693.333332,0.994704264,-0.007660433,0.978140045,0.009598961 +Cer(d18:1/14:0),139284.6667,148370,104714.6667,114694.6667,122697.3333,144298.6667,130789.7778,127230.2222,1.02797728,0.039808379,0.834535672,0.078555095 +Cer(d18:1/16:0),284413.3333,315025.3333,227298.6667,283726.6667,258184.6667,264832,275579.1111,268914.4445,1.024783595,0.035319286,0.815994986,0.08831251 +Cer(d18:1/18:0),40522,65140,47240.66667,67654,68190.66667,57741.33333,50967.55556,64528.66667,0.789843618,-0.340361054,0.169166304,0.771686139 +Cer(d18:1/18:1(9Z)),2018.666667,2966,1978,2572,2878.666667,2309.333333,2320.888889,2586.666667,0.897250859,-0.156416695,0.503855153,0.297694295 +Cer(d20:1/18:0),3302,5920.666667,3724,5168.666667,5550,4218,4315.555556,4978.888889,0.86677081,-0.206277526,0.503424877,0.298065327 +Choline,6098.666667,3817.333333,5502,6417,4596,6431.333333,5139.333333,5814.777778,0.883840024,-0.17814283,0.501541239,0.299693352 +Citrulline,313418,188764,198276.6667,122953.3333,100534,143415.3333,233486.2222,122300.8889,1.909113044,0.932902531,0.056882667,1.245020048 +CMP-2-aminoethylphosphonate,1936.666667,1870,2378.666667,2204.666667,2026,2788,2061.777778,2339.555556,0.881268997,-0.182345643,0.377351316,0.423254131 +Cyclohexaneundecanoic acid,4308.666667,4224.666667,4429.333333,4243.333333,4050.666667,3922.666667,4320.888889,4072.222222,1.06106412,0.085511841,0.087634384,1.057325463 +Cytidine,12657,5072,3504,9262.666667,4121,10274.66667,7077.666667,7886.111111,0.89748503,-0.15604022,0.824155687,0.08399074 +D-Asparagine,139858.6667,99435.33333,140345.3333,166586,148434.6667,207157.3333,126546.4444,174059.3333,0.727030502,-0.459912203,0.097190259,1.012377263 +Decanoyl-L-carnitine,6867.333333,2116,3414,9744,4046,10684.66667,4132.444444,8158.222223,0.506537372,-0.981259381,0.184320979,0.734425231 +Deoxyadenosine triphosphate,3824,3694,2964,2102.666667,2358,4217.333333,3494,2892.666667,1.207882,0.272479523,0.449521164,0.347249856 +DG(13:0/13:0/0:0),6962.666667,4868,5337.333333,5024,4960,6250,5722.666667,5411.333333,1.057533572,0.080703463,0.703372205,0.152814798 +DG(14:0/0:0/14:1n5),35236.66667,19544,35897.33333,29653.33333,23104.66667,29353.33333,30226,27370.44444,1.104329894,0.143171209,0.645787182,0.189910579 +DG(14:0/0:0/22:1n9),5850,7224,5567,5614,15268.66667,8710.666667,6213.666667,9864.444444,0.629905384,-0.666792952,0.275367839,0.560086784 +DG(14:1(9Z)/14:1(9Z)/0:0),40127.33333,22028.66667,44711.33333,32600.66667,29220.66667,34863.33333,35622.44444,32228.22222,1.10531832,0.14446191,0.65826134,0.18160165 +DG(15:0/0:0/14:1n5),2496.666667,1424,2532,1942,1535.333333,1535,2150.888889,1670.777778,1.287357851,0.364413139,0.283670982,0.547185088 +DG(15:0/a-17:0/0:0),1807.333333,1221,1144,1911,2215.333333,1373,1390.777778,1833.111111,0.758698024,-0.398402315,0.243062375,0.614282263 +DG(16:1(9Z)/18:1(11Z)/0:0),24141.33333,22295.33333,18706.66667,31310.66667,29276,23862.66667,21714.44444,28149.77778,0.771389551,-0.374468491,0.078332911,1.106055736 +DG(16:1n7/0:0/20:3n9),2241.333333,2155.333333,1156.666667,1256.666667,1606.666667,1021.333333,1851.111111,1294.888889,1.429552085,0.515563185,0.224425372,0.648928046 +DG(17:0/0:0/i-19:0),12822,7351.333333,23881.33333,15804.66667,11437,7168.666667,14684.88889,11470.11111,1.280274336,0.356452983,0.587895701,0.230699716 +DG(18:1n7/0:0/18:3n3),16985.33333,11395.33333,14797.33333,23950.66667,27775.33333,16787.33333,14392.66666,22837.77778,0.630213097,-0.666088358,0.079307252,1.100687099 +"DG(18:3(9Z,12Z,15Z)/14:1(9Z)/0:0)",2656,2946,6111.333333,8197.333333,5904.666667,7572,3904.444444,7224.666667,0.540432469,-0.887813741,0.063176966,1.199441237 +DG(22:0/0:0/16:1n7),18288,19566.66667,8234,7572.666667,58610.66667,16922.66667,15362.88889,27702,0.554576886,-0.850540607,0.485970232,0.313390332 +DG(22:0/18:1(11Z)/0:0),159791.3333,156673.3333,66642.66667,63362.66667,387030.6667,143856.6667,127702.4444,198083.3334,0.644690506,-0.633321357,0.528024436,0.277345979 +"DG(22:5(7Z,10Z,13Z,16Z,19Z)/14:0/0:0)",4664.666667,4214.666667,5333,7158.666667,9151.333333,5489.333333,4737.444444,7266.444444,0.651961834,-0.617140584,0.084407203,1.073620489 +DG(8:0/0:0/10:0),23160,19338.66667,21142.66667,30346,17748.66667,21522,21213.77778,23205.55556,0.914168063,-0.129468676,0.635812951,0.19667063 +DG(8:0/10:0/0:0),585473.3333,811771.3333,667944.6667,646650.6667,699430,581094.6667,688396.4444,642391.7778,1.071614657,0.099786218,0.570095691,0.244052242 +DG(8:0/14:0/0:0),8127.333333,7227.333333,7748.666667,8022.666667,6082.666667,6604.666667,7701.111111,6903.333334,1.11556414,0.157773464,0.277754521,0.556338863 +DG(i-12:0/0:0/12:0),2505.333333,2178,2752.666667,2136,2005.333333,2796.666667,2478.666667,2312.666667,1.071778611,0.10000693,0.604989278,0.218252322 +D-Glyceraldehyde 3-phosphate,4484,4248.666667,4281,5308.666667,5238.666667,8023,4337.888889,6190.111111,0.700777225,-0.512972206,0.114261598,0.942099707 +DL-Glycerol 1-phosphate,147417.3333,102918,154948,156541.3333,118442,188670.6667,135094.4444,154551.3333,0.874107273,-0.194117753,0.495721045,0.304762643 +D-Lysine,1202275,1321085,1378098,1291774,1243295.333,1380140.667,1300486,1305070,0.996487545,-0.005076322,0.947544946,0.02340018 +D-Proline,4541659.333,3476418,4958952,4433374.667,3587751.333,4476685.333,4325676.444,4165937.111,1.038344154,0.054284697,0.777233503,0.109448487 +D-Ribose 5-phosphate,10293.33333,6646,12664,11985.33333,9334,9004,9867.777777,10107.77778,0.976255909,-0.03466872,0.909748043,0.04107887 +FAHFA(16:0/7-O-18:0),3886,5810,3980.666667,3778,5516,6220,4558.888889,5171.333333,0.881569335,-0.181854053,0.557600156,0.253677114 +Flumiclorac-pentyl,3753.333333,2418,4744.666667,3955.333333,2612,1850.666667,3638.666667,2806,1.29674507,0.374894885,0.413196618,0.383843242 +Galactose 1-phosphate,311662.6667,306336.6667,376680.6667,306716.6667,280044,335050.6667,331560,307270.4444,1.079049437,0.109760964,0.429025266,0.36751713 +Galactosylceramide (d18:1/14:0),6506,9554,9177.333333,9800,15484.66667,13798.66667,8412.444444,13027.77778,0.645731343,-0.630994039,0.076026371,1.119035739 +Gamma-glutamyl-L-putrescine,1362,1238.666667,1723,2013.333333,1988.666667,1556.666667,1441.222222,1852.888889,0.777824418,-0.362483569,0.118419414,0.926577094 +gamma-Linolenic acid,11018,9708.666667,6486,3901,7955.333333,6292.666667,9070.888889,6049.666667,1.499403089,0.58438828,0.16639334,0.778864061 +Gartanin,4697.333333,3782,5593.333333,4335,3870,5141.333333,4690.888889,4448.777778,1.054421939,0.076452293,0.724981949,0.139672807 +GDP-glucose,6228,6148,6276.666667,6044.666667,4668,7746,6217.555556,6152.888889,1.010509968,0.015083553,0.945625094,0.024281011 +GlcCer(d18:1/16:0),22594.66667,23134,16998,23712.66667,26588,22662.66667,20908.88889,24321.11111,0.85970122,-0.218092742,0.209768422,0.678259888 +GlcCer(d18:1/9Z-18:1),13629.33333,14634.66667,16404.66667,13260,16916,17662,14889.55556,15946,0.933748624,-0.098893883,0.541237911,0.266611791 +Gln Thr Ala,2351,3885.333333,4871.333333,3625.333333,2571.333333,4213.333333,3702.555556,3470,1.067018892,0.09358572,0.803892122,0.094802228 +Glu Ala Arg,8792,10042,8480.666667,10068.66667,10834,10738,9104.888889,10546.88889,0.863277217,-0.21210418,0.054181502,1.266148959 +Glu Val Asp,15119.33333,14634.66667,12382,15639.33333,29319.33333,10827.33333,14045.33333,18595.33333,0.755314954,-0.404849745,0.462304589,0.335071795 +Glu Val Glu,23542,20721.33333,18616,25558.66667,31914,13991.33333,20959.77778,23821.33333,0.879874249,-0.184630745,0.626495379,0.203082128 +Glutamylcysteine,30650.66667,31164.66667,35107.33333,38531.33333,34083.33333,41671.33333,32307.55556,38095.33333,0.848071213,-0.237742681,0.09111179,1.040425422 +Glutamylglutamic acid,35424,43048,46498,44326,42427.33333,39330,41656.66667,42027.77778,0.991169861,-0.012795775,0.922447666,0.035058264 +Gly Glu Arg,19462,16140,17602,17681.33333,15490.66667,16884.66667,17734.66667,16685.55556,1.062875408,0.087972491,0.41506022,0.381888888 +Guanosine,1758,1430,1531.333333,1789.333333,960,1454.666667,1573.111111,1401.333333,1.122581668,0.166820405,0.544440872,0.264049279 +Hexaethylene glycol,38068,60521.33333,51875.33333,61665.33333,18340.66667,38096.66667,50154.88889,39367.55556,1.274015828,0.349383201,0.487664645,0.311878729 +His Phe Tyr Asp,3658,2806,2910.666667,3641.333333,3379.333333,4138.666667,3124.888889,3719.777778,0.840074079,-0.251411543,0.16314682,0.787421386 +Homocitric acid,52403,25947,48682.66667,39040,24094.66667,30234,42344.22222,31122.88889,1.360549221,0.44418915,0.295726633,0.529109561 +Homocitrulline,1400,1674.666667,820,905,1674,1736.666667,1298.222222,1438.555556,0.902448444,-0.148083581,0.721890431,0.141528715 +Ile Pro Pro,13067,13096.66667,13699.33333,12369.33333,13694,11826.66667,13287.66667,12630,1.052071787,0.073233148,0.328598131,0.483334912 +Imidazoleacetic acid riboside,4117,4143.333333,2580.666667,4215.333333,3322,6301.333333,3613.666667,4612.888889,0.783384719,-0.352207108,0.383896891,0.415785405 +Imidazoleacetic acid ribotide,2806,2421.333333,3347.333333,2782.666667,3082.666667,3100.666667,2858.222222,2988.666667,0.956353632,-0.064383911,0.673770824,0.171487799 +Indoleacetic acid,18622,10814,15465.33333,23008,16955.33333,25289.33333,14967.11111,21750.88889,0.688114917,-0.539278575,0.114045076,0.942923462 +Inositol cyclic phosphate,43854.66667,44384.66667,33902,43366.66667,49462,68976.66667,40713.77778,53935.11111,0.754865929,-0.405707663,0.192412374,0.715767001 +LacCer(d18:1/12:0),1903,844,1004,1073.333333,1121,1357.333333,1250.333333,1183.888889,1.056123885,0.078779076,0.855039995,0.068013571 +L-Asparagine,3977,2414,1812,2750.666667,2217.333333,3134,2734.333333,2700.666667,1.012466058,0.017873543,0.963831596,0.015998841 +L-Aspartic Acid,54941.33333,67758.66667,53105,68146.66667,72934.66667,72998,58601.66667,71359.77778,0.821214254,-0.284169426,0.059184273,1.227793683 +L-Carnitine,3158680.667,2210859.333,2995818,3487194,3055682.667,3350567.333,2788452.667,3297814.667,0.845545596,-0.242045542,0.185671261,0.731255314 +L-Glutamate,2958,1618.666667,3036,4246.666667,1926,3238.666667,2537.555556,3137.111111,0.808882907,-0.30599722,0.502368877,0.298977274 +L-Glutamic acid,3557533.333,2465462,2556575.333,2865092.667,2923211.333,2794332.667,2859856.889,2860878.889,0.999642767,-0.00051547,0.997821255,0.000947249 +L-hydroxy Arginine,48704,54462,64826,70341,49290.66667,90693.33333,55997.33333,70108.33333,0.798725782,-0.324227813,0.333793196,0.47652252 +Linoleic acid,101094,114258,62046.66667,21730,88744.66667,93360,92466.22222,67944.88889,1.360900338,0.444561418,0.429925348,0.366606948 +L-Norleucine,243460.6667,251891.3333,344518.6667,302200,263163.3333,311834,279956.8889,292399.1111,0.957447811,-0.062734245,0.744533076,0.128116004 +LPC 2:0,14804,13838.66667,21425.33333,19410.66667,14557.33333,15330,16689.33333,16432.66667,1.015619295,0.022359709,0.931853229,0.030652485 +L-Targinine,1830,1824,2534.666667,1924.666667,2090.666667,2696.666667,2062.888889,2237.333333,0.922030195,-0.117114098,0.627762827,0.202204405 +L-TYROSINE,811592,854004,1541320.667,1105350,847261.3333,848547.3333,1068972.222,933719.5555,1.14485363,0.195163161,0.619359682,0.208057069 +"LysoPA(22:2(13Z,16Z)/0:0)",6554,3926.666667,6068.666667,4437.333333,4147.333333,3987.333333,5516.444445,4190.666666,1.316364408,0.396558924,0.180308829,0.743983008 +LysoPC(0:0/16:0),267615.3333,187984,237452.6667,231019.3333,213910,248924,231017.3333,231284.4444,0.998845097,-0.001667136,0.992087277,0.00345012 +LysoPC(14:0/0:0),67312.66667,36311.33333,54152,49400.66667,40139.33333,51185.33333,52592,46908.44444,1.121162738,0.164995702,0.586174362,0.231973181 +LysoPC(14:1(9Z)/0:0),10324.66667,8648.666667,18033.33333,10609.33333,7238,13006.66667,12335.55556,10284.66667,1.199412286,0.262327656,0.572297226,0.242378359 +LysoPC(16:1(9Z)/0:0),375596.6667,245761.3333,346444.6667,296731.3333,256029.3333,365453.3333,322600.8889,306071.3333,1.054005566,0.075882486,0.760570289,0.118860644 +LysoPC(18:1(9Z)/0:0),233313.3333,160107.3333,213871.3333,201694,188932,263556,202430.6666,218060.6667,0.928322699,-0.1073017,0.64867485,0.18797294 +"LysoPC(18:2(9Z,12Z)/0:0)",77935.33333,58566,86811.33333,65031.33333,58876.66667,87591.33333,74437.55555,70499.77778,1.05585518,0.07841197,0.760629012,0.118827113 +"LysoPC(18:3(6Z,9Z,12Z)/0:0)",66003,51147,67840,77325,81915.33333,73395,61663.33333,77545.11111,0.795193049,-0.330622949,0.052765789,1.277647565 +LysoPE(0:0/14:1(9Z)),4972,2722.666667,3575.333333,2391.333333,2276,2475,3756.666667,2380.777778,1.577915714,0.658020144,0.10477019,0.97976227 +LysoPE(0:0/16:0),50447.33333,82512,80842.66667,71574,82699.33333,81252,71267.33333,78508.44444,0.907766468,-0.139606897,0.545995385,0.262811028 +LysoPE(0:0/18:1(11Z)),4190,3861.333333,4086,4534,3911.333333,3318,4045.777778,3921.111111,1.031793709,0.045154556,0.7493618,0.125308449 +"LysoPE(0:0/18:2(9Z,12Z))",153964,188310,207276.6667,144016.6667,166670,147636.6667,183183.5556,152774.4445,1.199045797,0.261886763,0.15017971,0.823388737 +LysoPE(14:0/0:0),21905.33333,19321.33333,26520.66667,16460.66667,17290.66667,17617.33333,22582.44444,17122.88889,1.31884547,0.399275533,0.062716454,1.202618503 +LysoPE(14:1(9Z)/0:0),2804.666667,1754,3396.666667,1706.666667,1799.333333,2022.666667,2651.777778,1842.888889,1.438924394,0.52499079,0.173704515,0.760188892 +LysoPE(16:0/0:0),167442,234460,230033.3333,195892.6667,208106,203733.3333,210645.1111,202577.3333,1.039825669,0.056341675,0.731623578,0.135712307 +LysoPE(16:1(9Z)/0:0),139934,104648,130744,112039.3333,102082,115524.6667,125108.6667,109882,1.138572893,0.187226658,0.249444565,0.603025955 +LysoPE(18:0/0:0),25604,37925.33333,34962.66667,31752.66667,37304,31843.33333,32830.66667,33633.33333,0.976134787,-0.034847723,0.855788048,0.067633783 +LysoPE(18:1(11Z)/0:0),148355.3333,181324,220318,169606.6667,166097.3333,153670,183332.4444,163124.6667,1.123879351,0.168487169,0.397540978,0.400618099 +LysoPI(16:0/0:0),4396,2194,2401.333333,1757,1649,2052.666667,2997.111111,1819.555556,1.647166585,0.719986469,0.173635929,0.760360405 +LysoPS(16:0/0:0),8635.333333,14438.66667,9515.333333,10462.66667,11462,13449.33333,10863.11111,11791.33333,0.921279282,-0.118289525,0.667884146,0.175298865 +LysoPS(18:1(9Z)/0:0),7206.666667,7740,6882,5648,7278,7670,7276.222222,6865.333333,1.059849809,0.083859835,0.57159715,0.242909945 +"LysoPS(18:2(9Z,12Z)/0:0)",16562.66667,23926,17651.33333,14460,17991.33333,17699.33333,19380,16716.88889,1.159306623,0.213262193,0.356699284,0.447697763 +Methylmalonic acid,4654,3166.666667,4956,5567.333333,4579.333333,5288.666667,4258.888889,5145.111111,0.827754503,-0.272725142,0.230023233,0.638228297 +MG(0:0/18:0/0:0),13870440,13331676,13713909.33,13872294.67,11587012,12393196.67,13638675.11,12617501.11,1.080933141,0.112277291,0.21192262,0.673822685 +MG(0:0/18:1(11Z)/0:0),4218,3379.333333,3894,4317.333333,2858.666667,3325.333333,3830.444444,3500.444444,1.094273743,0.129973687,0.541134843,0.266694501 +MG(0:0/20:0/0:0),114509.3333,104600.6667,104247.3333,106792,84112,84760,107785.7778,91888,1.173012556,0.230218456,0.123810682,0.907241885 +MG(0:0/i-18:0/0:0),4319.333333,3541.333333,3704,4166,3564,3320.666667,3854.888889,3683.555556,1.046513031,0.065590276,0.64582063,0.189888086 +MG(16:0/0:0/0:0),1291832,1515002,1271080,1270150.667,1134134,1099475.333,1359304.667,1167920,1.163867959,0.218927394,0.111056688,0.954455281 +MG(18:0/0:0/0:0),225880,184353.3333,234037.3333,235520.6667,167672,177726,214756.8889,193639.5556,1.109054853,0.149330722,0.464557851,0.332960196 +MG(18:1(11Z)/0:0/0:0),85368.66667,41178,82066,101948,54586,52862.66667,69537.55556,69798.88889,0.99625591,-0.005411718,0.990867827,0.003984273 +MG(22:1(13Z)/0:0/0:0),79606,61950,64482.66667,65436.66667,73594.66667,62156,68679.55556,67062.44444,1.024113513,0.034375633,0.815127783,0.088774304 +"MG(22:2(13Z,16Z)/0:0/0:0)",130832,72522,104654.6667,140187.3333,147391.3333,175962,102669.5556,154513.5555,0.664469568,-0.589724967,0.061299514,1.212542971 +MG(24:1(15Z)/0:0/0:0),245284,181863.3333,177286.6667,197019.3333,179272,141050.6667,201478,172447.3333,1.168345118,0.224466495,0.350075071,0.455838814 +MG(i-24:0/0:0/0:0),2812.666667,2313.333333,3862.666667,3495,2261.333333,3126,2996.222222,2960.777778,1.011971329,0.017168416,0.954585617,0.020185113 +Muramic acid,1610.666667,1248.666667,1272,2086.666667,1626,2706.666667,1377.111111,2139.777778,0.643576695,-0.635816009,0.084610699,1.072574717 +myo-Inositol 1-phosphate,66118.66667,38262,81403.33333,66573,53365.33333,75012.66667,61928,64983.66667,0.952977928,-0.069485295,0.839178372,0.076145718 +N2-Acetylornithine,3398.666667,4856,2701.333333,1141,1156.666667,5764,3652,2687.222222,1.359024189,0.442571134,0.593187152,0.226808264 +N-a-Acetyl-L-arginine,31341.33333,27530.66667,45791.33333,26384,18970,19110.66667,34887.77778,21488.22222,1.62357674,0.699175576,0.092128982,1.035603725 +N-Acetyl-a-neuraminic acid,1694.666667,1374.666667,1358.666667,1476.666667,1298,1811.333333,1476,1528.666667,0.965547318,-0.050581133,0.791150505,0.10174089 +N-Acetyl-b-glucosaminylamine,2244,1330,1660,2066,1647.333333,2394,1744.666667,2035.777778,0.857002511,-0.222628664,0.444641282,0.351990218 +N-Acetyl-D-mannosamine 6-phosphate,17602,16530,20766,19406,16515.33333,29068,18299.33333,21663.11111,0.844723237,-0.243449358,0.447991385,0.348730337 +N-Acetyl-L-glutamic acid,205618,240303.3333,223470.6667,219561.3333,210248,203122,223130.6667,210977.1111,1.057606038,0.080802319,0.334588949,0.475488407 +N-Acetylneuraminate 9-phosphate,198517.3333,92696,101642.6667,183634.6667,99170.66667,355428,130952,212744.4445,0.615536638,-0.700083364,0.378412593,0.422034419 +N-Acetylneuraminic acid,8486,43164,4568,22969.33333,50138.66667,4984.666667,18739.33333,26030.88889,0.719888338,-0.474154948,0.705577659,0.151455179 +N-Acetylneuraminic acid 9-phosphate,1415.333333,1015.333333,2204,1675.333333,1528,2160,1544.888889,1787.777778,0.864139217,-0.210664339,0.574631093,0.240610878 +NAD,98933.33333,89455.33333,94106,111232,84183.33333,113740,94164.88889,103051.7778,0.913762876,-0.130108264,0.41794045,0.378885594 +Octadecanamide,1690.666667,1473.333333,896,900,3108.666667,2264.666667,1353.333333,2091.111111,0.647183847,-0.627752495,0.342559086,0.465264508 +Octanoyl-L-Carnitine,143652.6667,3456,3786.666667,131109.3333,4639.333333,10132,50298.44446,48626.88888,1.034375129,0.048759492,0.979882092,0.008826179 +Octopamine (p-Hydroxyphenylethanolamine),55147.33333,72738.66667,44236,29630,41612,50294.66667,57374,40512.22222,1.416214585,0.50203988,0.174925536,0.757146787 +Octopine,18241,29203,68342,67652.66667,30013,75661.33333,38595.33333,57775.66667,0.668020562,-0.582035584,0.406973432,0.390433942 +Oleamide,36052,47773.33333,32711.33333,32917.33333,104451.3333,51194.66667,38845.55556,62854.44444,0.618024006,-0.694265217,0.335257746,0.474621179 +O-Phosphoethanolamine,1018475.333,879946,1005528.667,1133148.667,1078118.667,1320428,967983.3333,1177231.778,0.822253826,-0.282344279,0.070901451,1.149344879 +PA(10:0/a-15:0),9649.333333,3234,3270.666667,3562.666667,3985.333333,2426.666667,5384.666667,3324.888889,1.61950274,0.695550909,0.39873622,0.399314312 +PA(14:1(9Z)/14:0),2834,1932.666667,2381.333333,2462.666667,2059.333333,2560,2382.666667,2360.666667,1.009319401,0.013382791,0.945420533,0.02437497 +PA(15:0/14:1(9Z)),1033.333333,1077.333333,1528,1448,1004.666667,1374.666667,1212.888889,1275.777778,0.950705452,-0.072929661,0.778791269,0.108578926 +"PA(15:0/20:2(11Z,14Z))",307572,171742.6667,270940,219224,185162,232249.3333,250084.8889,212211.7778,1.178468469,0.236913159,0.427523789,0.369039714 +"PA(15:0/22:2(13Z,16Z))",333983.3333,193313.3333,218959.3333,206610,199824,252456,248752,219630,1.132595729,0.179632995,0.563529859,0.249083067 +PA(16:0/14:1(9Z)),5171.333333,4676,4910.666667,5146.666667,4472.666667,4497.333333,4919.333333,4705.555556,1.045430933,0.064097752,0.461891876,0.335459676 +"PA(18:0/20:2(11Z,14Z))",32809.33333,22642.66667,25268,19632.66667,20512,15033.33333,26906.66667,18392.66667,1.462901881,0.548833009,0.071169378,1.147706831 +"PA(18:2(9Z,12Z)/16:0)",103585.3333,125404.6667,112604,107527,105290.6667,87550,113864.6667,100122.5556,1.1372529,0.185553114,0.199255103,0.700590547 +"PA(18:3(9Z,12Z,15Z)/22:0)",23584.66667,13856.66667,18476.66667,11990.66667,13304.66667,7946,18639.33334,11080.44445,1.682182825,0.75033451,0.079943435,1.097217194 +"PA(20:2(11Z,14Z)/15:0)",131576.6667,66787.33333,104055.3333,74091.33333,61710.66667,96748.66667,100806.4444,77516.88889,1.300444921,0.379005297,0.337515606,0.471706141 +"PA(22:2(13Z,16Z)/15:0)",1017832,681555.3333,767238.6667,733632.6667,711434.6667,830730.6667,822208.6667,758599.3334,1.083851027,0.116166475,0.585310768,0.232613486 +PA(8:0/i-13:0),1246,987,1429,1590.666667,1300.666667,1574,1220.666667,1488.444444,0.820095551,-0.286136084,0.167427525,0.776173143 +"PA(i-12:0/18:2(9Z,11Z))",8008,5475.333333,8223.333333,5008,5448,5944.666667,7235.555555,5466.888889,1.323523434,0.404383739,0.12778297,0.893527022 +Palmitic amide,30644.66667,31115.33333,19296.66667,18518.66667,55201.33333,44202,27018.88889,39307.33333,0.687375271,-0.540830145,0.346757074,0.45997467 +PC(14:1(9Z)/14:1(9Z)),5654.666667,5047.333333,10316.66667,4847.333333,5332.666667,6122.666667,7006.222223,5434.222222,1.289277828,0.366563185,0.408826339,0.388461132 +PC(16:1(9Z)/14:0),4009.333333,4132.666667,7232,4836,3683.333333,4692.666667,5124.666667,4404,1.163639116,0.218643699,0.553245623,0.257082014 +PC(16:1(9Z)/18:1(11Z)),560121.3333,316244.6667,364553.3333,354874.6667,366133.3333,388355.3333,413639.7778,369787.7778,1.118586937,0.161677388,0.591107905,0.228333232 +PC(18:1(11Z)/16:0),496357.3333,378338,343024,369906.6667,371897.3333,414744.6667,405906.4444,385516.2222,1.052890698,0.074355676,0.696438471,0.157117247 +PC(18:1(9Z)/P-18:0),933.3333333,1647.333333,1060.666667,1165.333333,1158.666667,1424.666667,1213.777778,1249.555556,0.971367597,-0.041910733,0.887158635,0.051998716 +"PC(20:5(5Z,8Z,11Z,14Z,17Z)/16:1(9Z))",4500.666667,3630.666667,5241.333333,6036.666667,5527.333333,4798,4457.555556,5454,0.817300249,-0.291061922,0.165444383,0.781347972 +PC(O-16:0/20:0),1964,1687.333333,1576.666667,2046,1581,1299,1742.666667,1642,1.061307349,0.085842513,0.703763348,0.152573355 +PE(14:0/14:0),3141.333333,2851.333333,3257,3427.333333,3568,2165,3083.222222,3053.444444,1.009752192,0.014001278,0.951712119,0.0214944 +PE(14:0/16:0),14224,14109.33333,16679.33333,12363.33333,13094,12724.66667,15004.22222,12727.33333,1.178897561,0.237438362,0.05792803,1.237111242 +PE(15:0/14:0),9270,6244.666667,10718.66667,9148,7514.666667,9736,8744.444446,8799.555556,0.993737058,-0.009063929,0.972005027,0.012331489 +PE(15:0/14:1(9Z)),2649.333333,1742.666667,3563.333333,2041.333333,1518,2356.666667,2651.777778,1972,1.344714897,0.427300329,0.306010298,0.514263958 +"PE(15:0/18:2(9Z,12Z))",23953,21274,39712,25943.33333,17667.33333,26982,28313,23530.88889,1.203226964,0.266908803,0.500408796,0.300675065 +"PE(15:0/22:2(13Z,16Z))",1597010,988433.3333,1141028,1132358.667,1114617.333,1094068,1242157.111,1113681.333,1.115361346,0.157511179,0.521664444,0.282608763 +PE(16:1(9Z)/14:0),59095.33333,41276,44551.33333,34206.66667,33308,33776,48307.55555,33763.55556,1.43076032,0.516782013,0.056808813,1.245584285 +PE(18:1(9Z)/14:1(9Z)),29837.33333,22857.33333,24838,22993.33333,20408.66667,18385.33333,25844.22222,20595.77778,1.254831087,0.327493176,0.100599147,0.997405702 +PE(18:1(9Z)/15:0),146767,95004,151114,114263.3333,100836,128489.3333,130961.6667,114529.5556,1.143474853,0.193424638,0.45138249,0.345455292 +"PE(18:2(9Z,12Z)/P-16:0)",3074,4729.333333,2867.333333,3813.333333,3496.666667,4432,3556.888889,3914,0.908760575,-0.138027848,0.612022891,0.213232334 +PE(20:0/15:0),1002917.333,584992.6667,724852,775168,733828.6667,666097.3333,770920.6666,725031.3333,1.063292897,0.088539059,0.735873959,0.133196565 +"PE(20:2(11Z,14Z)/15:0)",2972.666667,2154,2212,2661.333333,2216,2254.666667,2446.222222,2377.333333,1.028977379,0.041211266,0.829509329,0.081178725 +PE(22:1(13Z)/14:1(9Z)),118920.6667,133203.3333,120124.6667,128116,130710,110916.6667,124082.8889,123247.5556,1.006777687,0.009745148,0.918971037,0.036698176 +"PE(DiMe(9,3)/MonoMe(13,5))",1978.666667,3864.666667,3042,3294,2302,4624.666667,2961.777778,3406.888889,0.869349684,-0.201991497,0.634533028,0.197545767 +PE-NMe(14:0/16:0),29515.33333,21522,33628.66667,28103.33333,23169.33333,32280.66667,28222,27851.11111,1.013316844,0.019085346,0.937206559,0.028164681 +PE-NMe(14:0/16:1(9Z)),29110.66667,22048.66667,40155.33333,29296,21362,30366.66667,30438.22222,27008.22222,1.126998363,0.172485419,0.597289333,0.223815241 +PE-NMe(14:1(9Z)/14:0),3545.333333,3206,7362,4508,3969.333333,4483.333333,4704.444444,4320.222222,1.088935754,0.12291884,0.789149692,0.102840609 +PE-NMe(14:1(9Z)/16:1(9Z)),4783.333333,5080,7541.333333,3774,3501.333333,4890.666667,5801.555556,4055.333333,1.430598937,0.516619275,0.146814412,0.83323131 +PE-NMe(15:0/14:1(9Z)),1141,2157,1931.333333,1407.333333,1050.666667,1435.333333,1743.111111,1297.777778,1.343150685,0.425621166,0.250870866,0.600549771 +PE-NMe(15:0/15:0),2386.666667,2344,4827.333333,2826,2009.333333,2892.666667,3186,2576,1.236801242,0.306613673,0.521175636,0.283015895 +PE-NMe(16:1(9Z)/16:0),3989.333333,3868.666667,6025.333333,3698.666667,3440.666667,4752.666667,4627.777778,3964,1.167451508,0.223362626,0.456759138,0.340312755 +PE-NMe(20:1(11Z)/14:1(9Z)),907033.3333,486712.6667,661669.3333,614881.3333,550855.3333,576845.3333,685138.4444,580860.6666,1.179522877,0.2382034,0.44537124,0.351277831 +PE-NMe(22:0/14:0),208310,121226,140264.6667,153630,155078.6667,168444,156600.2222,159050.8889,0.984591934,-0.022402175,0.931663755,0.0307408 +"PE-NMe(22:2(13Z,16Z)/16:0)",179283.3333,123844.6667,133130,138191.3333,130964,144032,145419.3333,137729.1111,1.055835852,0.07838556,0.683949777,0.164975788 +"PE-NMe(22:5(7Z,10Z,13Z,16Z,19Z)/16:1(9Z))",5069.333333,3462,5234.666667,5089.333333,5048.666667,5296.666667,4588.666667,5144.888889,0.89188839,-0.165064911,0.384812816,0.414750473 +PE-NMe2(14:0/14:1(9Z)),26499.33333,22118.66667,28611,21548,22719.33333,16233,25743,20166.77778,1.276505364,0.3521996,0.113808482,0.943825371 +PE-NMe2(14:0/18:1(11Z)),257952.6667,318855.3333,272548,318592.6667,286670,262682,283118.6667,289314.8889,0.97858312,-0.031233697,0.812657852,0.090092264 +PE-NMe2(16:0/18:1(11Z)),2014,2229.333333,2838,2898.666667,2393,2220,2360.444444,2503.888889,0.942711338,-0.085112016,0.677077785,0.169361435 +PE-NMe2(16:1(9Z)/16:0),83516.66667,116568,110564.6667,124812.6667,111101.3333,96630,103549.7778,110848,0.934160091,-0.098258283,0.605040733,0.218215387 +PE-NMe2(18:1(9Z)/16:1(9Z)),42633.33333,25606.66667,35669.33333,22772,23360,16962.66667,34636.44444,21031.55556,1.646879821,0.71973528,0.063692022,1.195914961 +PE-NMe2(20:0/15:0),502446.6667,313052,362081.3333,393779.3333,375332.6667,387386,392526.6667,385499.3333,1.018229171,0.026062303,0.907843224,0.041989144 +PG(16:0/16:1(9Z)),2136.666667,2156.666667,1948,1880,1842,2245,2080.444444,1989,1.045975085,0.064848487,0.561597383,0.250574925 +"PG(16:1(9Z)/18:3(6Z,9Z,12Z))",9203.333333,10362,7537.333333,7736.666667,8118,7987.333333,9034.222222,7947.333333,1.136761457,0.184929545,0.259243674,0.586291833 +"PG(16:1(9Z)/22:6(4Z,7Z,10Z,13Z,16Z,19Z))",2283.333333,3347.333333,3928,3238.666667,2452,3470.666667,3186.222222,3053.777778,1.043370688,0.061251809,0.828185136,0.081872569 +PG(i-12:0/a-15:0),6544,4269.333333,5884.666667,4474.666667,4580,3914.666667,5566,4323.111111,1.287498715,0.364570992,0.15337274,0.814251823 +PG(i-12:0/a-17:0),20788,14624.66667,22138.66667,17232.66667,15264.66667,14274.66667,19183.77778,15590.66667,1.230465521,0.299204232,0.219539349,0.658487629 +Phe Gly Ile,2812,5208.666667,8760,3369.333333,2303,6010,5593.555556,3894.111111,1.436413958,0.522471578,0.453547235,0.343377476 +Phenylacetylglycine,1750.666667,1181,2095,1782,2019,1623,1675.555556,1808,0.926745329,-0.109755156,0.671876991,0.172710232 +Phosphorylcholine; LC-tDDA; CE20,13286.66667,8668.666667,14140.66667,14826.66667,12566.66667,13117.33333,12032,13503.55556,0.891024586,-0.166462854,0.46656783,0.331085209 +"PI(16:0/16:2(9Z,12Z))",2497.333333,2574,2558,2190.666667,2260,2585.333333,2543.111111,2345.333333,1.084328217,0.116801514,0.1856021,0.731417114 +PI(16:0/18:0),892,1341.333333,1185.333333,1222,1414.666667,1272.666667,1139.555556,1303.111111,0.874488404,-0.193488842,0.318841672,0.496424922 +"PI(16:2(9Z,12Z)/18:0)",1432,1076,1226.666667,1359.333333,1176.666667,1274,1244.888889,1270,0.980227472,-0.028811515,0.839050666,0.076211814 +PI(18:1(11Z)/16:1(9Z)),8376.666667,10225.33333,9806,9756.666667,10093.33333,9181.333333,9469.333332,9677.11111,0.978528946,-0.031313567,0.754254843,0.122481892 +Pimelylcarnitine,2123,954.6666667,1904.666667,2670.666667,2123.333333,3592.666667,1660.777778,2795.555556,0.594077901,-0.751275971,0.11219047,0.950044032 +Pivaloylcarnitine,3888,1845.333333,3090,3026,2958,2578,2941.111111,2854,1.030522464,0.043375953,0.893430913,0.048939025 +PS(14:0/18:0),2697.333333,3664.666667,3003.333333,2810.666667,3176.666667,2743,3121.777778,2910.111111,1.072734909,0.101293606,0.539210917,0.268241323 +PS(14:0/18:1(11Z)),2660,3492,3622,3641.333333,3826.666667,4254,3258,3907.333333,0.833816755,-0.262197733,0.138615083,0.858189512 +PS(14:1(9Z)/18:1(9Z)),4034,3934.666667,4336,3320.666667,3584.666667,4019.333333,4101.555556,3641.555556,1.126319644,0.171616315,0.123952004,0.906746449 +PS(16:0/16:0),9558.666667,11262,11170.66667,9968.666667,11488,10729.33333,10663.77778,10728.66667,0.993951822,-0.008752171,0.931184062,0.030964466 +PS(16:1(9Z)/20:1(11Z)),28833.33333,32572,29956,32841.33333,33716,34726,30453.77778,33761.11111,0.902037189,-0.14874118,0.055243822,1.257716287 +PS(18:1(11Z)/18:1(9Z)),11700,21286.66667,13726,15150.66667,14794.66667,16159.33333,15570.88889,15368.22222,1.013187385,0.01890102,0.948448591,0.022986204 +PS(18:1(9Z)/14:0),2201,2257.333333,1773.333333,1530,1805.333333,1682.666667,2077.222222,1672.666667,1.241862628,0.312505595,0.078712121,1.103958388 +PS(18:1(9Z)/16:0),6930,9052,6472,7340,8054.666667,7906.666667,7484.666667,7767.111111,0.963635843,-0.053440038,0.749037427,0.125496482 +"PS(18:2(9Z,12Z)/16:0)",6707.333333,10912,9290.666667,8678,10662,12249.33333,8970,10529.77778,0.85186983,-0.231295099,0.385350006,0.41414463 +"PS(18:3(6Z,9Z,12Z)/16:0)",4416.666667,7347.333333,6350,6568,8176,11116,6038,8620,0.700464037,-0.513617112,0.178696279,0.747884492 +"PS(18:4(6Z,9Z,12Z,15Z)/22:2(13Z,16Z))",2360,2174,2671.333333,2701.333333,1542.666667,1859.333333,2401.777778,2034.444444,1.180557073,0.239467789,0.382714411,0.417125184 +PS(20:0/14:0),12093,18938,11724.66667,12387.33333,14399.33333,13696,14251.88889,13494.22222,1.056147487,0.078811316,0.76971624,0.11366935 +Pseudouridine,23497.33333,5137.333333,10903,30931.33333,10330,28184,13179.22222,23148.44444,0.569335113,-0.812650017,0.302547303,0.519206714 +S-(2-Methylpropionyl)-dihydrolipoamide-E,1550.666667,1724,1654,1639,1766,2162.666667,1642.888889,1855.888889,0.885230198,-0.175875428,0.267641399,0.572446708 +"Sedoheptulose 1,7-bisphosphate",18472,15465.33333,12595.33333,25571.33333,16668,36760.66667,15510.88889,26333.33333,0.589021097,-0.763608787,0.148421664,0.828502704 +Sedoheptulose 1-phosphate,17456,22200,33218.66667,19680.66667,21301.33333,26456,24291.55556,22479.33333,1.080617258,0.111855628,0.740097992,0.130710774 +Ser Ser Val,10297,9775.333333,11981,12618,12433.33333,14850,10684.44444,13300.44444,0.803314843,-0.315962561,0.062787363,1.202127759 +Ser Thr Leu,4933.333333,6566,8778,6939.333333,6040.666667,7375.333333,6759.111111,6785.111111,0.99616808,-0.00553891,0.98349462,0.007228011 +S-Glutaryldihydrolipoamide,888.6666667,956,1330,1057.333333,1149.333333,1034.666667,1058.222222,1080.444444,0.979432332,-0.029982273,0.882961956,0.054058008 +"SM(d17:2(4E,8Z)/16:0)",3400.666667,4873.333333,3557.333333,5320,4826.666667,4549.333333,3943.777778,4898.666667,0.805071675,-0.312810864,0.13933997,0.855924287 +SM(d18:1/15:0),23704.66667,36068.66667,24097.33333,31173.33333,32145.33333,27367.33333,27956.88889,30228.66666,0.924846908,-0.112713522,0.626120056,0.203342384 +sn-glycero-3-Phosphoethanolamine,899211.3333,575559.3333,688349.3333,1146993.333,802004,1163714,721040,1037570.444,0.694931129,-0.525058089,0.104590355,0.980508361 +Stearaldehyde,24598,23445.33333,14208,8569.333333,105205.3333,6625.333333,20750.44444,40133.33333,0.517037652,-0.951658749,0.585308404,0.23261524 +Taurine,546612,480426.6667,580535.3333,594326.6667,549429.3333,747392.6667,535858,630382.8889,0.850051626,-0.234377631,0.22965562,0.638922922 +TG(14:0/14:1(9Z)/16:1(9Z)),1196,1751.333333,2132,2673.333333,2120,1662.666667,1693.111111,2152,0.786761669,-0.346001424,0.314227124,0.50275633 +TG(14:0/14:1(9Z)/18:1(9Z)),1091.333333,1593.333333,1684,2090.666667,1755.333333,1560,1456.222222,1802,0.808114441,-0.307368481,0.224351547,0.649070932 +TG(14:0/14:1(9Z)/20:2n6),710,1274,1328,1996.666667,1542.666667,1002,1104,1513.777778,0.729301233,-0.455413262,0.305307792,0.515262111 +TG(14:1(9Z)/14:0/14:1(9Z)),942.6666667,1714.666667,2757.333333,2540,2039.333333,1723.333333,1804.888889,2100.888889,0.859107256,-0.219089837,0.635009053,0.197220083 +TG(14:1(9Z)/14:0/20:3n6),760.6666667,1233.333333,1282,1685.333333,1504,988.6666667,1092,1392.666667,0.784107228,-0.350877135,0.32281112,0.491051514 +TG(14:1(9Z)/14:1(9Z)/18:1(9Z)),1242,2042.666667,2294,2819.333333,2320,1506.666667,1859.555556,2215.333333,0.839402147,-0.252565942,0.513592742,0.289381123 +"TG(14:1(9Z)/14:1(9Z)/18:2(9Z,12Z))",919,1146.666667,1671.333333,1772.666667,1138.666667,1096,1245.666667,1335.777778,0.932540343,-0.100761956,0.787221482,0.103903063 +"TG(16:0/20:3(5Z,8Z,11Z)/14:1(9Z))",786,1072,1122,1664,1582.666667,821.3333333,993.3333333,1356,0.732546706,-0.449007349,0.276483098,0.558331413 +TG(8:0/10:0/10:0),1208,1936,3698.666667,1965.333333,1070.666667,2367.333333,2280.888889,1801.111111,1.266378779,0.340708985,0.595405613,0.225187075 +TG(8:0/13:0/i-12:0),2604.666667,3488,2021,2392.666667,2737,3290,2704.555556,2806.555556,0.963656518,-0.053409085,0.848355481,0.07142213 +TG(8:0/8:0/8:0),1637.333333,3623.333333,26598.66667,1904,1377,67782,10619.77778,23687.66667,0.448325195,-1.157382517,0.607168403,0.216690837 +Thr Thr Glu,5836.666667,9418.666667,4363.333333,2692.666667,3730,8940,6539.555556,5120.888889,1.277035237,0.352798334,0.593209188,0.226792131 +Threonic acid,8518.666667,12359,27684,15426,10526.66667,23903.33333,16187.22222,16618.66667,0.974038564,-0.037949202,0.954062331,0.020423251 +trans-2-Dodecenoylcarnitine,6899.333333,2760.666667,4334,10692,5846.666667,14031.33333,4664.666667,10190,0.457769055,-1.127308155,0.10679333,0.97145587 +Traumatic acid,2798,3479,3720.666667,4804,3600,3605.333333,3332.555556,4003.111111,0.832491396,-0.264492734,0.240159521,0.619500191 +UDP-D-galacturonic acid,3647.333333,3289,1807.333333,1910.666667,2408,3282,2914.555556,2533.555556,1.150381545,0.202112437,0.610856828,0.214060567 +UDP-N-acetyl-D-mannosamine,162077.3333,108898.6667,125510.6667,130358.6667,119662.6667,135976.6667,132162.2222,128666,1.027172852,0.038678978,0.841798224,0.074791995 +Uridine diphosphate glucose,57182.66667,41539.33333,41880,46106.66667,49815.33333,48922.66667,46867.33333,48281.55556,0.970708851,-0.042889448,0.802000134,0.095825559 +Uridine diphosphate-N-acetylgalactosamine,65016.66667,44378,51019.33333,46362,46894,56454,53471.33333,49903.33333,1.07149823,0.099629467,0.632849925,0.198699267 +Val Asn Ser,2667.333333,3287,2586.666667,3146.666667,3406.666667,2498,2847,3017.111111,0.943617883,-0.083725334,0.651651577,0.185984549 +Val Glu,2998,1611.333333,2564.666667,2340,1296.666667,1782,2391.333333,1806.222222,1.323941929,0.404839844,0.314007359,0.503060174 +Val Lys Glu,55546.66667,39692,55424,54572,50306.66667,62636,50220.88889,55838.22222,0.899399854,-0.152965446,0.428747421,0.36779848 +Xanthine,4765,7717.333333,53729,10305.33333,3050,11260,22070.44444,8205.111111,2.689841021,1.427520907,0.436712847,0.359804032 diff --git a/test_dataset.csv b/test_dataset.csv new file mode 100644 index 0000000..a2ca6c6 --- /dev/null +++ b/test_dataset.csv @@ -0,0 +1,19025 @@ +GeneNames,value1,value2,log2FC,p-value +LOC_Os09g01000.1,8862,32767,-1.88653936,1.25E-55 +LOC_Os12g42876.1,1099,117,3.231610951,1.05E-55 +LOC_Os12g42884.2,797,88,3.179004295,2.59E-54 +LOC_Os03g16920.1,274,7,5.290677161,4.69E-54 +LOC_Os05g47540.4,308,18,4.096861539,2.19E-54 +LOC_Os09g00999.1,339,1213,-1.839222372,1.95E-54 +LOC_Os01g62900.1,193,4,5.592457037,1.56E-54 +LOC_Os09g27030.2,504,162,1.637429921,1.36E-54 +LOC_Os01g51410.2,225,22,3.354349573,6.76E-54 +LOC_Os12g33946.1,343,79,2.118284018,1.03E-53 +LOC_Os06g50300.1,377,99,1.929064093,1.71E-53 +LOC_Os04g37619.2,200,14,3.836501268,9.62E-53 +LOC_Os08g39140.3,239,32,2.900866808,9.89E-51 +LOC_Os09g31130.1,136,0,NA,2.47E-48 +LOC_Os03g03720.1,196,666,-1.764668523,5.45E-41 +LOC_Os01g62290.1,186,24,2.95419631,1.45E-40 +LOC_Os02g02400.3,94,443,-2.236574037,1.85E-40 +LOC_Os08g09200.1,296,84,1.817135943,6.83E-40 +LOC_Os01g50030.1,168,22,2.932885804,2.26E-36 +LOC_Os09g29840.2,205,42,2.287162677,2.36E-35 +LOC_Os05g47540.1,114,4,4.832890014,9.23E-35 +LOC_Os02g02410.1,164,23,2.833990049,1.71E-34 +LOC_Os12g19381.1,65,329,-2.339575961,1.80E-32 +LOC_Os03g60080.1,120,11,3.447458977,6.22E-30 +LOC_Os06g40940.3,148,25,2.565597176,8.86E-29 +LOC_Os12g17600.1,75,329,-2.133125083,2.30E-28 +LOC_Os11g05050.1,515,291,0.823553279,6.32E-28 +LOC_Os05g39250.1,84,2,5.392317423,4.53E-27 +LOC_Os02g33710.1,170,41,2.051838932,1.25E-26 +LOC_Os03g17870.1,109,11,3.308752706,1.39E-26 +LOC_Os03g51600.1,36,224,-2.637429921,2.13E-26 +LOC_Os05g33960.1,94,6,3.969626351,6.46E-26 +LOC_Os04g38600.3,39,228,-2.547487795,1.19E-25 +LOC_Os08g41390.2,95,7,3.762500686,1.80E-25 +LOC_Os01g57960.1,167,493,-1.561739544,8.18E-25 +LOC_Os01g22010.2,119,18,2.724892762,1.06E-24 +LOC_Os05g02530.1,161,41,1.973364873,3.12E-24 +LOC_Os12g23200.1,63,276,-2.131244533,5.82E-24 +LOC_Os04g47220.1,118,19,2.634715536,9.47E-24 +LOC_Os09g26880.3,111,17,2.706953025,6.99E-23 +LOC_Os11g26850.2,158,44,1.84434913,2.42E-22 +LOC_Os04g32950.2,190,64,1.569855608,2.52E-22 +LOC_Os01g52250.4,501,315,0.669458775,1.05E-21 +LOC_Os01g31690.1,211,542,-1.361049853,6.94E-21 +LOC_Os07g14270.3,160,49,1.707218251,7.15E-21 +LOC_Os05g38530.1,88,10,3.137503524,1.18E-20 +LOC_Os02g51080.1,27,170,-2.654503434,1.42E-20 +LOC_Os02g27030.1,302,153,0.981016897,1.41E-20 +LOC_Os12g08770.1,37,194,-2.390459477,2.85E-20 +LOC_Os02g39360.1,158,49,1.689070904,3.26E-20 +LOC_Os07g34570.1,505,330,0.613817363,4.84E-20 +LOC_Os01g55540.2,110,345,-1.649092838,1.41E-19 +LOC_Os03g05310.1,71,5,3.827819025,1.72E-19 +LOC_Os04g28420.1,53,0,NA,2.82E-19 +LOC_Os03g16020.1,67,4,4.06608919,3.39E-19 +LOC_Os08g32870.1,138,41,1.750972452,1.17E-18 +LOC_Os10g30580.1,178,68,1.38827059,1.92E-18 +LOC_Os02g52390.1,264,133,0.989111684,2.63E-18 +LOC_Os03g49440.1,50,0,NA,3.17E-18 +LOC_Os01g49370.1,67,249,-1.893912742,4.48E-18 +LOC_Os11g13890.2,178,457,-1.360316924,8.49E-18 +LOC_Os01g33960.1,25,150,-2.584962501,1.57E-17 +LOC_Os08g38086.4,99,21,2.237039197,1.72E-17 +LOC_Os02g44870.1,85,14,2.602036014,3.14E-17 +LOC_Os03g46770.1,1074,913,0.234307228,4.60E-17 +LOC_Os01g08860.2,61,4,3.930737338,4.93E-17 +LOC_Os05g39990.1,0,63,NA,7.05E-17 +LOC_Os02g32504.1,65,6,3.437405312,1.11E-16 +LOC_Os03g57340.1,75,11,2.769387072,3.06E-16 +LOC_Os12g44360.2,126,40,1.655351829,3.41E-16 +LOC_Os01g20850.1,44,0,NA,3.99E-16 +LOC_Os08g31510.1,104,27,1.945552216,6.24E-16 +LOC_Os09g36220.1,124,40,1.632268215,9.46E-16 +LOC_Os05g44340.1,53,3,4.142957954,1.39E-15 +LOC_Os02g54130.3,89,20,2.153805336,2.78E-15 +LOC_Os05g46040.1,49,2,4.614709844,2.86E-15 +LOC_Os06g43660.3,119,328,-1.462734241,4.25E-15 +LOC_Os01g64660.2,17,115,-2.75802721,5.13E-15 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large collection of high-level mathematical functions to operate on these arrays. \n", + "- [pandas](https://pandas.pydata.org/) is a fast, powerful, flexible and easy to use open source data analysis and data manipulation library built on top of the Python programming language. \n", + "- [matplotlib](https://matplotlib.org/) is a plotting library for the Python programming language and its numerical mathematics extension NumPy.\n", + "- [seaborn](https://seaborn.pydata.org/) is a Python data visualization library based on matplotlib. It provides a high-level interface for drawing attractive and informative statistical graphics. \n", + "- [scipy](https://www.scipy.org/) is a free and open-source Python library used for scientific and technical computing. It is a collection of mathematical algorithms and convenience functions built on the NumPy extension of Python. \n", + "- [scikit-learn](https://scikit-learn.org/stable/) is a free software machine learning library for the Python programming language. It features various classification, regression and clustering algorithms including support vector machines, random forests, gradient boosting, k-means and DBSCAN, and is designed to interoperate with the Python numerical and scientific libraries NumPy and SciPy. " + ] + }, + { + "cell_type": "code", + "execution_count": 1, + "metadata": {}, + "outputs": [], + "source": [ + "# import libraries\n", + "import numpy as np\n", + "import pandas as pd\n", + "import matplotlib.pyplot as plt\n", + "import seaborn as sns\n", + "import dash_bio\n" + ] + }, + { + "cell_type": "markdown", + "metadata": {}, + "source": [ + "#### Visualizations\n", + "Full instructions can be found [here](https://hemtools.readthedocs.io/en/latest/content/Bioinformatics_Core_Competencies/Volcanoplot.html)\n" + ] + }, + { + "cell_type": "markdown", + "metadata": {}, + "source": [ + "##### Basic Plots" + ] + }, + { + "cell_type": "code", + "execution_count": 2, + "metadata": {}, + "outputs": [ + { + "data": { + "text/html": [ + "
\n", + " | Name | \n", + "Obp44a-/-_1 | \n", + "Obp44a-/-_2 | \n", + "Obp44a-/-_3 | \n", + "WT_1 | \n", + "WT_2 | \n", + "WT_3 | \n", + "7A Avg | \n", + "CS Avg | \n", + "FC | \n", + "Log2FC | \n", + "pval | \n", + "minuslog10(pval) | \n", + "
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
0 | \n", + "13-HODE | \n", + "6.652667e+03 | \n", + "8.484667e+03 | \n", + "4.238667e+03 | \n", + "2898.00000 | \n", + "2.331000e+03 | \n", + "2392.666667 | \n", + "6.458667e+03 | \n", + "2540.555556 | \n", + "2.542226 | \n", + "1.346092 | \n", + "0.034409 | \n", + "1.463326 | \n", + "
1 | \n", + "DG(i-14:0/0:0/13:0) | \n", + "2.812667e+03 | \n", + "2.066000e+03 | \n", + "2.966000e+03 | \n", + "1888.00000 | \n", + "1.489333e+03 | \n", + "1759.333333 | \n", + "2.614889e+03 | \n", + "1712.222222 | \n", + "1.527190 | \n", + "0.610880 | \n", + "0.040297 | \n", + "1.394723 | \n", + "
2 | \n", + "L-Phosphoarginine | \n", + "1.187180e+06 | \n", + "1.643805e+06 | \n", + "1.691681e+06 | \n", + "814283.33330 | \n", + "1.172822e+06 | \n", + "686245.333300 | \n", + "1.507556e+06 | \n", + "891116.888900 | \n", + "1.691760 | \n", + "0.758525 | \n", + "0.046787 | \n", + "1.329877 | \n", + "
3 | \n", + "LysoPE(0:0/20:0) | \n", + "4.179333e+03 | \n", + "3.395333e+03 | \n", + "4.147333e+03 | \n", + "2556.00000 | \n", + "2.978000e+03 | \n", + "1854.666667 | \n", + "3.907333e+03 | \n", + "2462.888889 | \n", + "1.586484 | \n", + "0.665833 | \n", + "0.025506 | \n", + "1.593356 | \n", + "
4 | \n", + "MG(22:0/0:0/0:0) | \n", + "5.464133e+04 | \n", + "5.547867e+04 | \n", + "5.519333e+04 | \n", + "41929.33333 | \n", + "3.487733e+04 | \n", + "37518.000000 | \n", + "5.510444e+04 | \n", + "38108.222220 | \n", + "1.445999 | \n", + "0.532066 | \n", + "0.001203 | \n", + "2.919832 | \n", + "