From dd13493a1e5f68ecc046a166fc17ee5d6963fb3b Mon Sep 17 00:00:00 2001 From: Pranjal-Bioinfo Date: Thu, 16 Jan 2025 14:26:43 +0530 Subject: [PATCH 01/26] Update modules.config Included Trimmomatic --- conf/modules.config | 19 +++++++++++++++++++ 1 file changed, 19 insertions(+) diff --git a/conf/modules.config b/conf/modules.config index 701598db..848525a5 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -47,6 +47,25 @@ process { tag = { "${meta.id}_run${meta.run}" } } + withName: TRIMMOMATIC { + ext.args = "LEADING:30 TRAILING:30 SLIDINGWINDOW:4:20 MINLEN:35" + publishDir = [ + [ + path: { "${params.outdir}/QC_shortreads/trimmomatic/${meta.id}" }, + mode: params.publish_dir_mode, + pattern: "*_trim.log" + ], + [ + path: { "${params.outdir}/QC_shortreads/trimmomatic/${meta.id}" }, + mode: params.publish_dir_mode, + pattern: "*.fastq.gz", + enabled: params.save_clipped_reads + ] + ] + ext.prefix = { "${meta.id}_run${meta.run}_trimmomatic" } + tag = { "${meta.id}_run${meta.run}" } + } + withName: ADAPTERREMOVAL_PE { ext.args = [ "--minlength ${params.reads_minlength}", From 2effc942376ae559ada6d4bba78b90c5447668ef Mon Sep 17 00:00:00 2001 From: Pranjal-Bioinfo Date: Thu, 16 Jan 2025 14:29:06 +0530 Subject: [PATCH 02/26] Update test.config Added clip tool as trimmmomatic. removed skip_concoct=true --- conf/test.config | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/conf/test.config b/conf/test.config index 04fced63..411cf0da 100644 --- a/conf/test.config +++ b/conf/test.config @@ -33,5 +33,5 @@ params { busco_clean = true skip_gtdbtk = true gtdbtk_min_completeness = 0.01 - skip_concoct = true + clip_tool = 'trimmomatic' } From aa94221bd3a2979d5a0520913b19bb65c9ae9635 Mon Sep 17 00:00:00 2001 From: Pranjal-Bioinfo Date: Thu, 16 Jan 2025 14:35:03 +0530 Subject: [PATCH 03/26] Update modules.json --- modules.json | 5 +++++ 1 file changed, 5 insertions(+) diff --git a/modules.json b/modules.json index 05e3b3dd..a83e743a 100644 --- a/modules.json +++ b/modules.json @@ -282,6 +282,11 @@ "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", "installed_by": ["modules"] }, + "trimmomatic": { + "branch": "master", + "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1", + "installed_by": ["modules"] + }, "untar": { "branch": "master", "git_sha": "5caf7640a9ef1d18d765d55339be751bb0969dfa", From 94e9f54257c63c04bc8433af2f887fdd8093f4a9 Mon Sep 17 00:00:00 2001 From: Pranjal-Bioinfo Date: Thu, 16 Jan 2025 14:38:41 +0530 Subject: [PATCH 04/26] Create Trimmomatic --- modules/nf-core/Trimmomatic | 1 + 1 file changed, 1 insertion(+) create mode 100644 modules/nf-core/Trimmomatic diff --git a/modules/nf-core/Trimmomatic b/modules/nf-core/Trimmomatic new file mode 100644 index 00000000..8b137891 --- /dev/null +++ b/modules/nf-core/Trimmomatic @@ -0,0 +1 @@ + From 088207e207c56e9552fea3ec21f5a4188781526a Mon Sep 17 00:00:00 2001 From: Pranjal-Bioinfo Date: Thu, 16 Jan 2025 19:35:44 +0530 Subject: [PATCH 05/26] Delete modules/nf-core/Trimmomatic --- modules/nf-core/Trimmomatic | 1 - 1 file changed, 1 deletion(-) delete mode 100644 modules/nf-core/Trimmomatic diff --git a/modules/nf-core/Trimmomatic b/modules/nf-core/Trimmomatic deleted file mode 100644 index 8b137891..00000000 --- a/modules/nf-core/Trimmomatic +++ /dev/null @@ -1 +0,0 @@ - From f5effeb9c7954daf01effe15d2d33b42bd4a3766 Mon Sep 17 00:00:00 2001 From: Pranjal-Bioinfo Date: Thu, 16 Jan 2025 19:37:52 +0530 Subject: [PATCH 06/26] Create environment.yml --- modules/nf-core/Trimmomatic/environment.yml | 5 +++++ 1 file changed, 5 insertions(+) create mode 100644 modules/nf-core/Trimmomatic/environment.yml diff --git a/modules/nf-core/Trimmomatic/environment.yml b/modules/nf-core/Trimmomatic/environment.yml new file mode 100644 index 00000000..ab4b72b7 --- /dev/null +++ b/modules/nf-core/Trimmomatic/environment.yml @@ -0,0 +1,5 @@ +channels: + - conda-forge + - bioconda +dependencies: + - bioconda::trimmomatic=0.39 From c4bf5e4c4f770f1f79de1ab9e555788ca1809b8a Mon Sep 17 00:00:00 2001 From: Pranjal-Bioinfo Date: Thu, 16 Jan 2025 19:39:04 +0530 Subject: [PATCH 07/26] Create main.nf --- modules/nf-core/Trimmomatic/main.nf | 71 +++++++++++++++++++++++++++++ 1 file changed, 71 insertions(+) create mode 100644 modules/nf-core/Trimmomatic/main.nf diff --git a/modules/nf-core/Trimmomatic/main.nf b/modules/nf-core/Trimmomatic/main.nf new file mode 100644 index 00000000..ee500d85 --- /dev/null +++ b/modules/nf-core/Trimmomatic/main.nf @@ -0,0 +1,71 @@ +process TRIMMOMATIC { + tag "$meta.id" + label 'process_medium' + + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/trimmomatic:0.39--hdfd78af_2': + 'biocontainers/trimmomatic:0.39--hdfd78af_2' }" + + input: + tuple val(meta), path(reads) + + output: + tuple val(meta), path("*.paired.trim*.fastq.gz") , emit: trimmed_reads + tuple val(meta), path("*.unpaired.trim_*.fastq.gz"), emit: unpaired_reads, optional:true + tuple val(meta), path("*_trim.log") , emit: trim_log + tuple val(meta), path("*_out.log") , emit: out_log + tuple val(meta), path("*.summary") , emit: summary + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + def trimmed = meta.single_end ? "SE" : "PE" + def output = meta.single_end ? + "${prefix}.SE.paired.trim.fastq.gz" // HACK to avoid unpaired and paired in the trimmed_reads output + : "${prefix}.paired.trim_1.fastq.gz ${prefix}.unpaired.trim_1.fastq.gz ${prefix}.paired.trim_2.fastq.gz ${prefix}.unpaired.trim_2.fastq.gz" + def qual_trim = task.ext.args2 ?: '' + """ + trimmomatic \\ + $trimmed \\ + -threads $task.cpus \\ + -trimlog ${prefix}_trim.log \\ + -summary ${prefix}.summary \\ + $reads \\ + $output \\ + $qual_trim \\ + $args 2> >(tee ${prefix}_out.log >&2) + cat <<-END_VERSIONS > versions.yml + "${task.process}": + trimmomatic: \$(trimmomatic -version) + END_VERSIONS + """ + + stub: + def prefix = task.ext.prefix ?: "${meta.id}" + + if (meta.single_end) { + output_command = "echo '' | gzip > ${prefix}.SE.paired.trim.fastq.gz" + } else { + output_command = "echo '' | gzip > ${prefix}.paired.trim_1.fastq.gz" + output_command = "echo '' | gzip > ${prefix}.paired.trim_2.fastq.gz" + output_command += "echo '' | gzip > ${prefix}.unpaired.trim_1.fastq.gz" + output_command += "echo '' | gzip > ${prefix}.unpaired.trim_2.fastq.gz" + } + + """ + $output_command + touch ${prefix}.summary + touch ${prefix}_trim.log + touch ${prefix}_out.log + cat <<-END_VERSIONS > versions.yml + "${task.process}": + trimmomatic: \$(trimmomatic -version) + END_VERSIONS + """ + +} From 42fef907f60c019c418097a8e0342c035f850da1 Mon Sep 17 00:00:00 2001 From: Pranjal-Bioinfo Date: Thu, 16 Jan 2025 19:39:45 +0530 Subject: [PATCH 08/26] Create meta.yml --- modules/nf-core/Trimmomatic/meta.yml | 85 ++++++++++++++++++++++++++++ 1 file changed, 85 insertions(+) create mode 100644 modules/nf-core/Trimmomatic/meta.yml diff --git a/modules/nf-core/Trimmomatic/meta.yml b/modules/nf-core/Trimmomatic/meta.yml new file mode 100644 index 00000000..e74014d5 --- /dev/null +++ b/modules/nf-core/Trimmomatic/meta.yml @@ -0,0 +1,85 @@ +name: "trimmomatic" +description: Performs quality and adapter trimming on paired end and single end reads +keywords: + - trimming + - adapter trimming + - quality trimming +tools: + - "trimmomatic": + description: "A flexible read trimming tool for Illumina NGS data" + homepage: "http://www.usadellab.org/cms/?page=trimmomatic" + documentation: "https://github.com/usadellab/Trimmomatic" + doi: "10.1093/bioinformatics/btu170" + licence: ["GPL v3"] + identifier: biotools:trimmomatic +input: + - - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - reads: + type: file + description: | + Input FastQ files of size 1 or 2 for single-end and paired-end data, respectively. + pattern: "*.fastq.gz" +output: + - trimmed_reads: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - "*.paired.trim*.fastq.gz": + type: file + description: The trimmed/modified paired end fastq reads + pattern: "*.paired.trim*.fastq.gz" + - unpaired_reads: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - "*.unpaired.trim_*.fastq.gz": + type: file + description: The trimmed/modified unpaired end fastq reads + pattern: "*.unpaired.trim_*.fastq.gz" + - trim_log: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - "*_trim.log": + type: file + description: trimmomatic log file, from the trim_log parameter + pattern: "*.log" + - out_log: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - "*_out.log": + type: file + description: log of output from the standard out + pattern: "*.log" + - summary: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - "*.summary": + type: file + description: trimmomatic summary file of surviving and dropped reads + pattern: "*.summary" + - versions: + - versions.yml: + type: file + description: File containing software versions + pattern: "versions.yml" +authors: + - "@alyssa-ab" +maintainers: + - "@alyssa-ab" From 42f99061d9e21f1abf54ae2f84cf8efbc12d0e55 Mon Sep 17 00:00:00 2001 From: Pranjal-Bioinfo Date: Thu, 16 Jan 2025 19:41:17 +0530 Subject: [PATCH 09/26] Create main.nf.test --- .../nf-core/Trimmomatic/tests/main.nf.test | 118 ++++++++++++++++++ 1 file changed, 118 insertions(+) create mode 100644 modules/nf-core/Trimmomatic/tests/main.nf.test diff --git a/modules/nf-core/Trimmomatic/tests/main.nf.test b/modules/nf-core/Trimmomatic/tests/main.nf.test new file mode 100644 index 00000000..fee08848 --- /dev/null +++ b/modules/nf-core/Trimmomatic/tests/main.nf.test @@ -0,0 +1,118 @@ +nextflow_process { + + name "Test Process TRIMMOMATIC" + script "../main.nf" + process "TRIMMOMATIC" + tag "modules" + tag "modules_nfcore" + tag "trimmomatic" + + test("Single-Read") { + config "./nextflow_SE.config" + when { + process { + """ + input[0] = [ + [ id: 'test', single_end:true ], + [ + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) + ] + ] + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert process.out.trimmed_reads != null }, + { assert process.out.trimmed_reads.get(0).get(1) ==~ ".*.SE.paired.trim.fastq.gz" }, + { assert process.out.out_log.get(0).get(1) ==~ ".*.log" }, + { assert snapshot(process.out.trimmed_reads, + process.out.trim_log, + process.out.summary, + process.out.versions + ).match() } + ) + } + } + + test("Paired-Reads") { + config "./nextflow_PE.config" + when { + process { + """ + input[0] = [ + [ id: 'test', single_end:false ], + [ + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true) + ] + ] + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert process.out.trimmed_reads != null }, + { assert process.out.trimmed_reads.get(0).get(1).get(0) ==~ ".*.paired.trim_1.fastq.gz" }, + { assert process.out.trimmed_reads.get(0).get(1).get(1) ==~ ".*.paired.trim_2.fastq.gz" }, + { assert process.out.out_log.get(0).get(1) ==~ ".*.log" }, + { assert snapshot(process.out.trimmed_reads, + process.out.trim_log, + process.out.summary, + process.out.versions + ).match() } + ) + } + } + + test("No Adaptors") { + + when { + process { + """ + input[0] = [ + [ id: 'test', single_end:false ], + [ + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true) + ] + ] + """ + } + } + + then { + assertAll ( + { assert process.failed } + ) + } + } + + test("Single-Read - stub") { + options "-stub" + when { + process { + """ + input[0] = [ + [ id: 'test', single_end:true ], + [ + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) + ] + ] + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + } + +} From 4da22b67e07fff718922287170f6efa7aa897721 Mon Sep 17 00:00:00 2001 From: Pranjal-Bioinfo Date: Thu, 16 Jan 2025 19:42:19 +0530 Subject: [PATCH 10/26] Create main.nf.test.snap --- .../Trimmomatic/tests/main.nf.test.snap | 178 ++++++++++++++++++ 1 file changed, 178 insertions(+) create mode 100644 modules/nf-core/Trimmomatic/tests/main.nf.test.snap diff --git a/modules/nf-core/Trimmomatic/tests/main.nf.test.snap b/modules/nf-core/Trimmomatic/tests/main.nf.test.snap new file mode 100644 index 00000000..6b51bc4e --- /dev/null +++ b/modules/nf-core/Trimmomatic/tests/main.nf.test.snap @@ -0,0 +1,178 @@ +{ + "Single-Read - stub": { + "content": [ + { + "0": [ + [ + { + "id": "test", + "single_end": true + }, + "test.SE.paired.trim.fastq.gz:md5,68b329da9893e34099c7d8ad5cb9c940" + ] + ], + "1": [ + + ], + "2": [ + [ + { + "id": "test", + "single_end": true + }, + "test_trim.log:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "3": [ + [ + { + "id": "test", + "single_end": true + }, + "test_out.log:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "4": [ + [ + { + "id": "test", + "single_end": true + }, + "test.summary:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "5": [ + "versions.yml:md5,14413a048f088a147fb04f3d59c6c604" + ], + "out_log": [ + [ + { + "id": "test", + "single_end": true + }, + "test_out.log:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "summary": [ + [ + { + "id": "test", + "single_end": true + }, + "test.summary:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "trim_log": [ + [ + { + "id": "test", + "single_end": true + }, + "test_trim.log:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "trimmed_reads": [ + [ + { + "id": "test", + "single_end": true + }, + "test.SE.paired.trim.fastq.gz:md5,68b329da9893e34099c7d8ad5cb9c940" + ] + ], + "unpaired_reads": [ + + ], + "versions": [ + "versions.yml:md5,14413a048f088a147fb04f3d59c6c604" + ] + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.04.2" + }, + "timestamp": "2024-06-26T21:50:12.135007267" + }, + "Single-Read": { + "content": [ + [ + [ + { + "id": "test", + "single_end": true + }, + "test.SE.paired.trim.fastq.gz:md5,e68abbd3b88f7ec12940a4f5c2b8bfb9" + ] + ], + [ + [ + { + "id": "test", + "single_end": true + }, + "test_trim.log:md5,e4c3f619e9b0e26847f8f3e3d9af319b" + ] + ], + [ + [ + { + "id": "test", + "single_end": true + }, + "test.summary:md5,24c973237557a1439c775ca19a5deaa5" + ] + ], + [ + "versions.yml:md5,14413a048f088a147fb04f3d59c6c604" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.04.2" + }, + "timestamp": "2024-06-26T21:56:07.085100348" + }, + "Paired-Reads": { + "content": [ + [ + [ + { + "id": "test", + "single_end": false + }, + [ + "test.paired.trim_1.fastq.gz:md5,a866e64f451745f176316d0df7d52b30", + "test.paired.trim_2.fastq.gz:md5,725d4ab909b39291ab56b090cab32075" + ] + ] + ], + [ + [ + { + "id": "test", + "single_end": false + }, + "test_trim.log:md5,9629761761a34576b3484bf4174f681f" + ] + ], + [ + [ + { + "id": "test", + "single_end": false + }, + "test.summary:md5,9698e5e5c060bbe64588998fe35f8d71" + ] + ], + [ + "versions.yml:md5,14413a048f088a147fb04f3d59c6c604" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.04.2" + }, + "timestamp": "2024-06-26T21:56:18.779303181" + } +} From 574ff5c1d689119ca5e39896e3d6e889f6f10f62 Mon Sep 17 00:00:00 2001 From: Pranjal-Bioinfo Date: Thu, 16 Jan 2025 19:43:03 +0530 Subject: [PATCH 11/26] Create nextflow_PE.config --- modules/nf-core/Trimmomatic/tests/nextflow_PE.config | 6 ++++++ 1 file changed, 6 insertions(+) create mode 100644 modules/nf-core/Trimmomatic/tests/nextflow_PE.config diff --git a/modules/nf-core/Trimmomatic/tests/nextflow_PE.config b/modules/nf-core/Trimmomatic/tests/nextflow_PE.config new file mode 100644 index 00000000..3f8fdfe1 --- /dev/null +++ b/modules/nf-core/Trimmomatic/tests/nextflow_PE.config @@ -0,0 +1,6 @@ +process { + + withName: TRIMMOMATIC { + ext.args = 'ILLUMINACLIP:TruSeq3-PE.fa:2:30:10 LEADING:3 TRAILING:3 SLIDINGWINDOW:4:15 MINLEN:36' + } +} From 97626596586afea4edb74f1c62c362b47e3db53c Mon Sep 17 00:00:00 2001 From: Pranjal-Bioinfo Date: Thu, 16 Jan 2025 19:43:43 +0530 Subject: [PATCH 12/26] Create nextflow_SE.config --- modules/nf-core/Trimmomatic/tests/nextflow_SE.config | 6 ++++++ 1 file changed, 6 insertions(+) create mode 100644 modules/nf-core/Trimmomatic/tests/nextflow_SE.config diff --git a/modules/nf-core/Trimmomatic/tests/nextflow_SE.config b/modules/nf-core/Trimmomatic/tests/nextflow_SE.config new file mode 100644 index 00000000..af777fda --- /dev/null +++ b/modules/nf-core/Trimmomatic/tests/nextflow_SE.config @@ -0,0 +1,6 @@ +process { + + withName: TRIMMOMATIC { + ext.args = 'ILLUMINACLIP:TruSeq3-SE:2:30:10 LEADING:3 TRAILING:3 SLIDINGWINDOW:4:15 MINLEN:36' + } +} From 72c03d53569bd0eb102600dbd8ddcb269c520865 Mon Sep 17 00:00:00 2001 From: Pranjal-Bioinfo Date: Thu, 16 Jan 2025 19:44:15 +0530 Subject: [PATCH 13/26] Create tags.yml --- modules/nf-core/Trimmomatic/tests/tags.yml | 2 ++ 1 file changed, 2 insertions(+) create mode 100644 modules/nf-core/Trimmomatic/tests/tags.yml diff --git a/modules/nf-core/Trimmomatic/tests/tags.yml b/modules/nf-core/Trimmomatic/tests/tags.yml new file mode 100644 index 00000000..68c5af6a --- /dev/null +++ b/modules/nf-core/Trimmomatic/tests/tags.yml @@ -0,0 +1,2 @@ +trimmomatic: + - modules/nf-core/trimmomatic/** From 2327da571fd966745ea6cf804c5c8c6aa007ccd8 Mon Sep 17 00:00:00 2001 From: Pranjal-Bioinfo Date: Thu, 16 Jan 2025 19:45:50 +0530 Subject: [PATCH 14/26] Update nextflow_schema.json --- nextflow_schema.json | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/nextflow_schema.json b/nextflow_schema.json index 18307b05..bef8834f 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -269,7 +269,7 @@ "type": "string", "default": "fastp", "description": "Specify which adapter clipping tool to use.", - "enum": ["fastp", "adapterremoval"] + "enum": ["fastp", "adapterremoval", "trimmomatic"] }, "save_clipped_reads": { "type": "boolean", From 911da74fb4361fd809f0c47a42ae534b5e317adb Mon Sep 17 00:00:00 2001 From: Pranjal-Bioinfo Date: Thu, 16 Jan 2025 19:46:43 +0530 Subject: [PATCH 15/26] Update shortread_preprocessing.nf --- subworkflows/local/shortread_preprocessing.nf | 1 + 1 file changed, 1 insertion(+) diff --git a/subworkflows/local/shortread_preprocessing.nf b/subworkflows/local/shortread_preprocessing.nf index ad33b56f..421cb81d 100644 --- a/subworkflows/local/shortread_preprocessing.nf +++ b/subworkflows/local/shortread_preprocessing.nf @@ -5,6 +5,7 @@ include { FASTQC as FASTQC_RAW } from '../../modules/nf-core/fastqc/main' include { FASTQC as FASTQC_TRIMMED } from '../../modules/nf-core/fastqc/main' include { FASTP } from '../../modules/nf-core/fastp/main' +include { TRIMMOMATIC } from '../../modules/nf-core/trimmomatic/main' include { ADAPTERREMOVAL as ADAPTERREMOVAL_PE } from '../../modules/nf-core/adapterremoval/main' include { ADAPTERREMOVAL as ADAPTERREMOVAL_SE } from '../../modules/nf-core/adapterremoval/main' include { BOWTIE2_REMOVAL_BUILD as BOWTIE2_HOST_REMOVAL_BUILD } from '../../modules/local/bowtie2_removal_build' From 0f324b7b7fa482b9b468eff989232b22c60f1b8f Mon Sep 17 00:00:00 2001 From: Pranjal-Bioinfo Date: Thu, 16 Jan 2025 19:49:42 +0530 Subject: [PATCH 16/26] Update shortread_preprocessing.nf --- subworkflows/local/shortread_preprocessing.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/subworkflows/local/shortread_preprocessing.nf b/subworkflows/local/shortread_preprocessing.nf index 421cb81d..961d62ec 100644 --- a/subworkflows/local/shortread_preprocessing.nf +++ b/subworkflows/local/shortread_preprocessing.nf @@ -5,7 +5,7 @@ include { FASTQC as FASTQC_RAW } from '../../modules/nf-core/fastqc/main' include { FASTQC as FASTQC_TRIMMED } from '../../modules/nf-core/fastqc/main' include { FASTP } from '../../modules/nf-core/fastp/main' -include { TRIMMOMATIC } from '../../modules/nf-core/trimmomatic/main' +include { TRIMMOMATIC } from '../../modules/nf-core/Trimmomatic/main' include { ADAPTERREMOVAL as ADAPTERREMOVAL_PE } from '../../modules/nf-core/adapterremoval/main' include { ADAPTERREMOVAL as ADAPTERREMOVAL_SE } from '../../modules/nf-core/adapterremoval/main' include { BOWTIE2_REMOVAL_BUILD as BOWTIE2_HOST_REMOVAL_BUILD } from '../../modules/local/bowtie2_removal_build' From 68b1383838e50ccdc39c78d7d69cb16cb14634b5 Mon Sep 17 00:00:00 2001 From: Pranjal-Bioinfo Date: Thu, 16 Jan 2025 19:51:03 +0530 Subject: [PATCH 17/26] Update shortread_preprocessing.nf --- subworkflows/local/shortread_preprocessing.nf | 10 ++++++++++ 1 file changed, 10 insertions(+) diff --git a/subworkflows/local/shortread_preprocessing.nf b/subworkflows/local/shortread_preprocessing.nf index 961d62ec..586ee286 100644 --- a/subworkflows/local/shortread_preprocessing.nf +++ b/subworkflows/local/shortread_preprocessing.nf @@ -65,6 +65,16 @@ workflow SHORTREAD_PREPROCESSING { ch_multiqc_files = ch_multiqc_files.mix(ADAPTERREMOVAL_PE.out.settings) ch_multiqc_files = ch_multiqc_files.mix(ADAPTERREMOVAL_SE.out.settings) } + else if (params.clip_tool == 'trimmomatic') { + + TRIMMOMATIC(ch_raw_short_reads) + + ch_short_reads_prepped = Channel.empty() + ch_short_reads_prepped = TRIMMOMATIC.out.trimmed_reads + + ch_versions = ch_versions.mix(TRIMMOMATIC.out.versions.first()) + ch_multiqc_files = ch_multiqc_files.mix(TRIMMOMATIC.out.summary) + } } else { ch_short_reads_prepped = ch_raw_short_reads From 881f554f7760de1024d9959f1c9d7a5055e0a8e7 Mon Sep 17 00:00:00 2001 From: Pranjal-Bioinfo Date: Thu, 16 Jan 2025 19:51:48 +0530 Subject: [PATCH 18/26] Update mag.nf --- workflows/mag.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/workflows/mag.nf b/workflows/mag.nf index de353a40..231afcf9 100644 --- a/workflows/mag.nf +++ b/workflows/mag.nf @@ -380,7 +380,7 @@ workflow MAG { if (!params.single_end && !params.skip_spades) { METASPADES(ch_short_reads_spades.map { meta, reads -> [meta, reads, [], []] }, [], []) - ch_spades_assemblies = METASPADES.out.scaffolds.map { meta, assembly -> + ch_spades_assemblies = METASPADES.out.contigs.map { meta, assembly -> def meta_new = meta + [assembler: 'SPAdes'] [meta_new, assembly] } From af5adac67c07598de10c5ceb6a228fb6ae8b3f66 Mon Sep 17 00:00:00 2001 From: Pranjal-Bioinfo Date: Fri, 17 Jan 2025 12:03:02 +0530 Subject: [PATCH 19/26] Update modules.config added ext.args ='c 10000 -o 0 --merge_last at 642 --- conf/modules.config | 3 +++ 1 file changed, 3 insertions(+) diff --git a/conf/modules.config b/conf/modules.config index 848525a5..62e7949d 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -639,6 +639,9 @@ process { ] } + withName: 'CONCOCT_CUTUPFASTA' { + ext.args = '-c 10000 -o 0 --merge_last' + } withName: 'CONCOCT_.*' { publishDir = [ [ From b8f944d3781ba700324d0b79ea187334e08bde0b Mon Sep 17 00:00:00 2001 From: Pranjal-Bioinfo Date: Fri, 17 Jan 2025 13:00:03 +0530 Subject: [PATCH 20/26] Renamed folder from Trimmomatic to trimmomatic --- assets/nf-core-mag_logo_light.png | Bin 67534 -> 76588 bytes docs/images/nf-core-mag_logo_light.png | Bin 20607 -> 23432 bytes .../environment.yml | 0 .../{Trimmomatic => trimmomatic}/main.nf | 0 .../{Trimmomatic => trimmomatic}/meta.yml | 0 .../tests/main.nf.test | 0 .../tests/main.nf.test.snap | 0 .../tests/nextflow_PE.config | 0 .../tests/nextflow_SE.config | 0 .../tests/tags.yml | 0 subworkflows/local/shortread_preprocessing.nf | 2 +- 11 files changed, 1 insertion(+), 1 deletion(-) rename modules/nf-core/{Trimmomatic => trimmomatic}/environment.yml (100%) rename modules/nf-core/{Trimmomatic => 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rename from modules/nf-core/Trimmomatic/tests/main.nf.test rename to modules/nf-core/trimmomatic/tests/main.nf.test diff --git a/modules/nf-core/Trimmomatic/tests/main.nf.test.snap b/modules/nf-core/trimmomatic/tests/main.nf.test.snap similarity index 100% rename from modules/nf-core/Trimmomatic/tests/main.nf.test.snap rename to modules/nf-core/trimmomatic/tests/main.nf.test.snap diff --git a/modules/nf-core/Trimmomatic/tests/nextflow_PE.config b/modules/nf-core/trimmomatic/tests/nextflow_PE.config similarity index 100% rename from modules/nf-core/Trimmomatic/tests/nextflow_PE.config rename to modules/nf-core/trimmomatic/tests/nextflow_PE.config diff --git a/modules/nf-core/Trimmomatic/tests/nextflow_SE.config b/modules/nf-core/trimmomatic/tests/nextflow_SE.config similarity index 100% rename from modules/nf-core/Trimmomatic/tests/nextflow_SE.config rename to modules/nf-core/trimmomatic/tests/nextflow_SE.config diff --git a/modules/nf-core/Trimmomatic/tests/tags.yml b/modules/nf-core/trimmomatic/tests/tags.yml similarity index 100% rename from modules/nf-core/Trimmomatic/tests/tags.yml rename to modules/nf-core/trimmomatic/tests/tags.yml diff --git a/subworkflows/local/shortread_preprocessing.nf b/subworkflows/local/shortread_preprocessing.nf index 586ee286..f3822e1c 100644 --- a/subworkflows/local/shortread_preprocessing.nf +++ b/subworkflows/local/shortread_preprocessing.nf @@ -5,7 +5,7 @@ include { FASTQC as FASTQC_RAW } from '../../modules/nf-core/fastqc/main' include { FASTQC as FASTQC_TRIMMED } from '../../modules/nf-core/fastqc/main' include { FASTP } from '../../modules/nf-core/fastp/main' -include { TRIMMOMATIC } from '../../modules/nf-core/Trimmomatic/main' +include { TRIMMOMATIC } from '../../modules/nf-core/trimmomatic/main' include { ADAPTERREMOVAL as ADAPTERREMOVAL_PE } from '../../modules/nf-core/adapterremoval/main' include { ADAPTERREMOVAL as ADAPTERREMOVAL_SE } from '../../modules/nf-core/adapterremoval/main' include { 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zCJ5kz!KyTAx(1XLfcL1h7I`6&jGNb>J}^375)~E2uq8>TFb6{xellLtXXiVW>E9It z2uFiVI}+OvI9tsPJ}7?R#bI~qSQrH@&R#zh9{NUeMe4EKrI6*b>9f|z=;BV~ApqQ9x`q|m;tZ6JY zbHw}FF-}OR6zDWovWL*6AJgURApl(H;|q6R+q&^ed7uY18vZk#-%=zmZ>{<^olpw_ zD+P)k;N|-D+{%n!2_gmMOL0bWP5lYWNG^8PzHU2 zbF?ai77rZU3bqsj;NR_ylEVug(@f2^yqAulvzz+E0GurZm`zJ4^8DI# z5cYsO8QuAKAo@m1PWz&-7&yB~4i^r`sHD5~3&o9gC^Wv3$Eh$wD?_}3X<*4h)+fzY z8x6PnOO_Zlc4_>jb_*o{c^3CxKwbqhVhrdXAq-jLcrF@%fK$anS+B_>FJ&K#mI|d$vChZLnmx~!POB&Z9UQ};My}8 z@1A|(TQ<|9uMRPn$GlU~0ol18uCDO8vK-A-pULtkCpP)M0=dq*CR^S2cN@^tRWIWr zh-*I3)*fTlTI2yC-xb2rDGBb_aWq*^ku}nyk*`qZ*PO|}m8Z^yU8<|J^`mIMgxc64 zxt35E5ta*uh9#}JythI%i-h6yOIs|=E&~DZ_W=&<(f>+vzvC1V$>1kvS+?;6g4rij z*i|6)iY;XYEQoqV|3@NsSx(P2e_~}(R+hp31$qX{5T;^~R)t5b2Fe}_bBA=R&;hBa z4d^=la%dsEF9V2Z%<%w@>9$O*ej)APyWtSVen31A0T>?uT2|)Jki7#ESEqOu0O2x5 zL&tBbwWlZFYM46~F$~pBv=p8R4Iy&;Km4( Date: Mon, 20 Jan 2025 20:19:00 +1300 Subject: [PATCH 22/26] Removed test config change --- conf/test.config | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/conf/test.config b/conf/test.config index 411cf0da..04fced63 100644 --- a/conf/test.config +++ b/conf/test.config @@ -33,5 +33,5 @@ params { busco_clean = true skip_gtdbtk = true gtdbtk_min_completeness = 0.01 - clip_tool = 'trimmomatic' + skip_concoct = true } From b1eb9ed8e735daee753b4e73bf92e19c3d074760 Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Mon, 20 Jan 2025 20:30:03 +1300 Subject: [PATCH 23/26] Added parameter spades_use_contigs_not_scaffolds --- nextflow.config | 1 + nextflow_schema.json | 4 ++++ workflows/mag.nf | 9 +++++---- 3 files changed, 10 insertions(+), 4 deletions(-) diff --git a/nextflow.config b/nextflow.config index 387fee34..b7ac54e8 100644 --- a/nextflow.config +++ b/nextflow.config @@ -59,6 +59,7 @@ params { // assembly options coassemble_group = false spades_options = null + spades_use_contigs_not_scaffolds = false megahit_options = null skip_spades = false skip_spadeshybrid = false diff --git a/nextflow_schema.json b/nextflow_schema.json index bef8834f..a3fd6a35 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -570,6 +570,10 @@ "description": "Additional custom options for SPAdes and SPAdesHybrid. Do not specify `--meta` as this will be added for you!", "help_text": "An example is adjusting k-mers (\"-k 21,33,55,77\") or adding [advanced options](https://github.com/ablab/spades#advanced-options). But not --meta, -t, -m, -o or --out-prefix, because these are already in use. Must be used like this: --spades_options \"-k 21,33,55,77\")" }, + "spades_use_contigs_not_scaffolds": { + "type": "boolean", + "description": "Specify whether to use contigs or scaffolds assembled by SPAdes" + }, "megahit_options": { "type": "string", "description": "Additional custom options for MEGAHIT.", diff --git a/workflows/mag.nf b/workflows/mag.nf index 231afcf9..709d1a6b 100644 --- a/workflows/mag.nf +++ b/workflows/mag.nf @@ -380,10 +380,11 @@ workflow MAG { if (!params.single_end && !params.skip_spades) { METASPADES(ch_short_reads_spades.map { meta, reads -> [meta, reads, [], []] }, [], []) - ch_spades_assemblies = METASPADES.out.contigs.map { meta, assembly -> - def meta_new = meta + [assembler: 'SPAdes'] - [meta_new, assembly] - } + ch_spades_assemblies = ( params.spades_use_contigs_not_scaffolds ? METASPADES.out.contigs : METASPADES.out.scaffolds ) + .map { meta, assembly -> + def meta_new = meta + [assembler: 'SPAdes'] + [meta_new, assembly] + } ch_assembled_contigs = ch_assembled_contigs.mix(ch_spades_assemblies) ch_versions = ch_versions.mix(METASPADES.out.versions.first()) } From 5d8518e4be9d8d5d9ca7235d749b63db72fd3111 Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Mon, 20 Jan 2025 20:33:42 +1300 Subject: [PATCH 24/26] Fixed pre-commit --- conf/modules.config | 2 +- subworkflows/local/shortread_preprocessing.nf | 2 +- 2 files changed, 2 insertions(+), 2 deletions(-) diff --git a/conf/modules.config b/conf/modules.config index 62e7949d..8e913744 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -47,7 +47,7 @@ process { tag = { "${meta.id}_run${meta.run}" } } - withName: TRIMMOMATIC { + withName: TRIMMOMATIC { ext.args = "LEADING:30 TRAILING:30 SLIDINGWINDOW:4:20 MINLEN:35" publishDir = [ [ diff --git a/subworkflows/local/shortread_preprocessing.nf b/subworkflows/local/shortread_preprocessing.nf index f3822e1c..b71c47c2 100644 --- a/subworkflows/local/shortread_preprocessing.nf +++ b/subworkflows/local/shortread_preprocessing.nf @@ -65,7 +65,7 @@ workflow SHORTREAD_PREPROCESSING { ch_multiqc_files = ch_multiqc_files.mix(ADAPTERREMOVAL_PE.out.settings) ch_multiqc_files = ch_multiqc_files.mix(ADAPTERREMOVAL_SE.out.settings) } - else if (params.clip_tool == 'trimmomatic') { + else if (params.clip_tool == 'trimmomatic') { TRIMMOMATIC(ch_raw_short_reads) From 815681c6d594d7fea4d3b0c95e940cc768bb7e8d Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Mon, 20 Jan 2025 20:43:12 +1300 Subject: [PATCH 25/26] Restored logos from dev --- assets/nf-core-mag_logo_light.png | Bin 65555 -> 67534 bytes docs/images/nf-core-mag_logo_light.png | Bin 20508 -> 20607 bytes 2 files changed, 0 insertions(+), 0 deletions(-) diff --git a/assets/nf-core-mag_logo_light.png b/assets/nf-core-mag_logo_light.png index 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zZwV=U6qU`a>z}lS`R(7-z%UKKRr#@i|C{UP_spA66_;rqcZdfcaNw+&c63g|!ElD5kS!y^tae4bt5|-7PXOOT{&5gRKMJVZ+2dIN!Zul=)y|inccwL zc5epMsOJLMHlNO8y_A(@YX#9iI+K3$5^{PHsg4E#Tm4stAEeoQvTN|8GRH1_P#S>Ao*8Ny@7Sw!g1FrVkgn)d;Sl%Q+k2468=66bg7yR22T+KM54zp z*+x{yqI(%$`v|h;qc14{EurAjq`(PU9)s7^5TRO;symWZY$hjlNaRafMvqxU1>X!h z831l}WNDg9F?JxNc6+)GfuSA7Lg(s%X$P2 Date: Mon, 20 Jan 2025 09:51:06 +0000 Subject: [PATCH 26/26] update changelog --- CHANGELOG.md | 8 +++++++- 1 file changed, 7 insertions(+), 1 deletion(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index 004375b5..a80ea5db 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -3,14 +3,20 @@ The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/) and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html). -## dev [unreleased] +## 3.3.1dev [unreleased] ### `Added` +- [#745](https://github.com/nf-core/mag/pull/745) - Added `trimmomatic` as an additional pre-processing tool (added by @Pranjal-Bioinfo & @GallVp). + ### `Changed` +- [#745](https://github.com/nf-core/mag/pull/745) - Added pipeline parameter `spades_use_contigs_not_scaffolds` to use contigs instead of scaffolds (added by @Pranjal-Bioinfo & @GallVp). + ### `Fixed` +- [#745](https://github.com/nf-core/mag/pull/745) - Set `concoct/cut_up_fasta.py` default params in `modules.config` (added by @Pranjal-Bioinfo & @GallVp). + ### `Dependencies` ### `Dependencies`