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Inclusion criteria for MAGs when setting up custom database #104

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adityabandla opened this issue Nov 6, 2019 · 0 comments
Open

Inclusion criteria for MAGs when setting up custom database #104

adityabandla opened this issue Nov 6, 2019 · 0 comments

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@adityabandla
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Hi Stephen,
Thanks for the great tool. I have a set of species-level MAGs that range in completeness from 50-100% and redundancy/contamination 0-10%. These are species representatives chosen using dRep with a genome average ANI cutoff of 95%

Since these are environmental MAGs, I would like to construct my own database. Given the above numbers, can I include all MAGs or is it better to include only MAGs that are substantially complete say >70%? Also, to what extent does redundancy affect downstream steps such as calling SNPs?

Best,
Adi

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