This template performs some basic preprocessing, clustering and dimension reduction steps on flow cytometry data.
- Preprocessing: channel-specific logicle transformation
- Clustering using PhenoGraph
- Dimension Reduction using UMAP
- Marker enrichment score
- Cell population (cluster) proportions computation across samples
- Relevant data visualisations
- An FCS File or a ZIP of multiple FCS files (imported using the "Upload File" button)
- A Sample Annotation file containing at least two columns: "filename" and "Condition"