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Process multi_sample_wgs:recalibrate_base:run_ApplyBQSR_GATK (30) terminated with an error exit status (3) #103

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graceooh opened this issue Aug 25, 2022 · 2 comments
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@graceooh
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Describe the issue
Pipeline crashes for one of my samples
Says in log file:
Process multi_sample_wgs:recalibrate_base:run_ApplyBQSR_GATK (30) terminated with an error exit status (3)

  • Pipeline release version- 9.1.0

  • Cluster you are using (SGE/Slurm-Dev/Slurm-Test)

  • Node type (F2s (lowmem) / F72s (midmem) / M64s (execute)) - F72

  • Submission method (interactive/submission script) - submission script

  • Actual submission script (python submission script, "nextflow run ...", etc.)

    python /hot/user/jieunoh/tools/tool-submit-nf/submit_nextflow_pipeline.py
    --nextflow_script /hot/software/pipeline/pipeline-call-gSNP/Nextflow/release/9.1.0/main.nf
    --sge_scheduler False
    --multi_node_mode False
    --nextflow_config "/hot/user/jieunoh/gap6_pilot/call-gSNP-input/2021-9127_bone/input_config_dir/BE-4.config"
    --pipeline_run_name BE-4-call-gsnp
    --partition_type F72
    --email [email protected]

  • Sbatch or qsub command and logs if applicable

  • Config files: /hot/user/jieunoh/gap6_pilot/call-gSNP-input/2021-9127_bone/input_config_dir/BE-4.config

  • Path to the working directory

  • Any logs produced by the pipeline:

/hot/user/jieunoh/gap6_pilot/call-gSNP-input/2021-9127_bone/log_files/BE-4-Bone/ BE-4-call-gsnp.log

To Reproduce
Steps to reproduce the behavior:

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  2. Click on '....'
  3. Scroll down to '....'
  4. See error

Expected behavior
Pipeline crashes possibly due to memory error

Screenshots
Screen Shot 2022-08-25 at 2 07 40 PM
Screen Shot 2022-08-25 at 2 08 01 PM

Additional context
Add any other context about the problem here.

@yashpatel6
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The issue seems to be disk space; the scratch space is running out. Could you try this run with a higher value for gatk_ir_compression? This controls the compression level of the output files produced by Indel Realignment; the default is 0 for fastest runtime but it also produced the least compressed outputs. Maybe try 5 since that's usually the current default GATK uses.

@yashpatel6 yashpatel6 self-assigned this Aug 25, 2022
@graceooh
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Thank you! I'll let you know how it goes. :D

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