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Hi, I used umis cb_histogram to calculate reads count for each cell and then chose a count cutoff based on that. For the downstream analysis, such as pseudo-align and count UMI, we only need to deal with reads from those cells. Is there any function for this based on umis cb_histogram results? Thanks.
The text was updated successfully, but these errors were encountered:
Sorry for not responding sooner. There isn't functionality for the FASTQ file, but there is from an alignment file. umis subset_bamfile will subset a BAM file and keep only the given cellular barcodes.
Hi, I used
umis cb_histogram
to calculate reads count for each cell and then chose a count cutoff based on that. For the downstream analysis, such as pseudo-align and count UMI, we only need to deal with reads from those cells. Is there any function for this based onumis cb_histogram
results? Thanks.The text was updated successfully, but these errors were encountered: