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is there any function to extract fastq for certain cells passing the filter? #44

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lixin4306ren opened this issue Sep 6, 2017 · 1 comment

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@lixin4306ren
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Hi, I used umis cb_histogram to calculate reads count for each cell and then chose a count cutoff based on that. For the downstream analysis, such as pseudo-align and count UMI, we only need to deal with reads from those cells. Is there any function for this based on umis cb_histogram results? Thanks.

@roryk
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roryk commented Oct 11, 2017

Hi Xin,

Sorry for not responding sooner. There isn't functionality for the FASTQ file, but there is from an alignment file. umis subset_bamfile will subset a BAM file and keep only the given cellular barcodes.

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