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10x scRNA-seq #14

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doctorjb77 opened this issue Nov 6, 2020 · 1 comment
Open

10x scRNA-seq #14

doctorjb77 opened this issue Nov 6, 2020 · 1 comment

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@doctorjb77
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Hello,

Thanks for this resource.
I have a scRNA-seq dataset with close to 10,000 cells from droplet based sequencing (10x chromium).
How does the pipeline handle large number of cells from droplet based sequencing?

Thanks!
Brian

@zhouzilu
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Hi Brian,
Unfortunately, from our research, 10X based (5' or 3') scRNA-seq don't work well as only a tiny portion of the genome will be captured of very low depth...
Zilu

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