New Novaseq X constants

Clinical-Genomics · Nov 22, 2024 · 025d7e9 · 025d7e9
1 parent 5f5db08
commit 025d7e9
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Showing 2 changed files with 6 additions and 3 deletions.
diff --git a/cg_lims/EPPs/udf/calculate/constants.py b/cg_lims/EPPs/udf/calculate/constants.py
@@ -21,6 +21,7 @@ class FlowCellLaneVolumes10B(FloatEnum):
     """The recommended volume of reagents per 10B flow cell lane. All values are in ul."""
 
     POOL_VOLUME: float = 35
+    DENATURATION_VOLUME: float = 34
     PHIX_VOLUME: float = 1
     NAOH_VOLUME: float = 8.5
     BUFFER_VOLUME: float = 127.5
@@ -30,6 +31,7 @@ class FlowCellLaneVolumes15B(FloatEnum):
     """The recommended volume of reagents per 1.5B flow cell lane. All values are in ul."""
 
     POOL_VOLUME: float = 35
+    DENATURATION_VOLUME: float = 34
     PHIX_VOLUME: float = 1
     NAOH_VOLUME: float = 8.5
     BUFFER_VOLUME: float = 127.5
@@ -39,6 +41,7 @@ class FlowCellLaneVolumes25B(FloatEnum):
     """The recommended volume of reagents per 25B flow cell lane. All values are in ul."""
 
     POOL_VOLUME: float = 57
+    DENATURATION_VOLUME: float = 56
     PHIX_VOLUME: float = 1.6
     NAOH_VOLUME: float = 14
     BUFFER_VOLUME: float = 210

diff --git a/cg_lims/EPPs/udf/calculate/novaseq_x_denaturation.py b/cg_lims/EPPs/udf/calculate/novaseq_x_denaturation.py
@@ -53,19 +53,19 @@ def get_reagent_list(self):
 
 DENATURATION_VOLUMES = {
     FlowCellTypes.FLOW_CELL_10B: NovaSeqXDenaturation(
-        pool=FlowCellLaneVolumes10B.POOL_VOLUME,
+        pool=FlowCellLaneVolumes10B.DENATURATION_VOLUME,
         phix=FlowCellLaneVolumes10B.PHIX_VOLUME,
         naoh=FlowCellLaneVolumes10B.NAOH_VOLUME,
         buffer=FlowCellLaneVolumes10B.BUFFER_VOLUME,
     ),
     FlowCellTypes.FLOW_CELL_15B: NovaSeqXDenaturation(
-        pool=FlowCellLaneVolumes15B.POOL_VOLUME,
+        pool=FlowCellLaneVolumes15B.DENATURATION_VOLUME,
         phix=FlowCellLaneVolumes15B.PHIX_VOLUME,
         naoh=FlowCellLaneVolumes15B.NAOH_VOLUME,
         buffer=FlowCellLaneVolumes15B.BUFFER_VOLUME,
     ),
     FlowCellTypes.FLOW_CELL_25B: NovaSeqXDenaturation(
-        pool=FlowCellLaneVolumes25B.POOL_VOLUME,
+        pool=FlowCellLaneVolumes25B.DENATURATION_VOLUME,
         phix=FlowCellLaneVolumes25B.PHIX_VOLUME,
         naoh=FlowCellLaneVolumes25B.NAOH_VOLUME,
         buffer=FlowCellLaneVolumes25B.BUFFER_VOLUME,