New Novaseq X volume constants

[idalindegaard]

A patch after the change in issue #522

Added

• new denaturation volume constants in constants.py
• updated which constants to use in novaseq_x_denaturation.py

Changed

Fixed

Steps to consider while deploying

• Configuration changes:
• Documentation updates:
• Inform users by email:

Review:

• Code approved by
• Tests executed on stage by: (Document the test done with screen shots and description.)
• "Merge and deploy" approved by

This version is a:

• MAJOR - when you make incompatible API changes
• MINOR - when you add functionality in a backwards compatible manner
• PATCH - when you make backwards compatible bug fixes or documentation/instructions

[idalindegaard]

Testing on stage:

Expected result

We want the Adjusted Bulk Pool Volume (ul) in step Define Run Format and Calculate Volumes (NovaSeq X) to be 35 ul as a minimum volume per lane for 1.5B and 10B flowcells, and 57 ul per lane for a 25B flowcell. In step Make Pool and Denature (NovaSeq X) the Total Volume of Pool to Denature (ul) should reflect the value of Adjusted Bulk Pool Volume (ul) in the previous step, while Volume of Pool to Denature (ul) per Lane should be 34 ul for 1.5B and 10B flowcells, and 56 ul for a 25B flowcell, (the actual pipetting volume).

Result

1.5B flowcell for 1 lane:

10B flowcell for 1 lane:

25B flowcell for 1 lane:

10B flowcell for 8 lanes

Conclusion:

Expected result was achieved.

[Karl-Svard]

Nicely done!