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RNA-seq of GR manipulation in A549 cells

This repository contains the RNA-seq processing and differential expression analysis for the paper Gazorpak*, Hugentobler* et al., "Harnessing PROTAC technology to combat stress hormone receptor activation".

Briefly, a novel custom-made GR-PROTAC is compared to 2 common inhibitors (Mifepristone and cort113176) both at baseline and upon Dex-mediated GR activation, demonstrating a higher specificity and superior blockage.

The code for the primary processing of the RNA-seq and differential expression analyses is available here, the results of which are available as a SummarizedExperiment here. The code for further downstream analyses, including the figures from the paper, are available in the docs.

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