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example_data/example_config_files/config_training_golgi.yml
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--- | ||
name: "Cellpose Segmentation" | ||
input_channels: 3 | ||
output_channels: 5 | ||
ShardedCytosolSegmentationDownsamplingCellpose: | ||
input_channels: 2 | ||
output_masks: 2 | ||
shard_size: 120000000 # maxmimum number of pixel per tile | ||
overlap_px: 100 | ||
nGPUs: 2 | ||
chunk_size: 50 # chunk size for chunked HDF5 storage. is needed for correct caching and high performance reading. should be left at 50. | ||
threads: 1 # number of shards / tiles segmented at the same size. should be adapted to the maximum amount allowed by memory. | ||
cache: "/fs/pool/pool-mann-maedler-shared/temp" | ||
lower_quantile_normalization: 0.001 | ||
upper_quantile_normalization: 0.999 | ||
median_filter_size: 6 # Size in pixels | ||
nucleus_segmentation: | ||
model: "nuclei" | ||
cytosol_segmentation: | ||
model: "cyto2" | ||
chunk_size: 50 | ||
filtering_threshold: 0.95 | ||
downsampling_factor: 4 | ||
smoothing_kernel_size: 7 | ||
HDF5CellExtraction: | ||
compression: True | ||
threads: 80 # threads used in multithreading | ||
image_size: 128 # image size in pixel | ||
normalization_range: None #turn of percentile normalization for cells -> otherwise normalise out differences for the alexa647 channel | ||
cache: "/fs/pool/pool-mann-maedler-shared/temp" | ||
hdf5_rdcc_nbytes: 5242880000 # 5gb 1024 * 1024 * 5000 | ||
hdf5_rdcc_w0: 1 | ||
hdf5_rdcc_nslots: 50000 |