Preparation Steps for Whole Slide Images (WSIs) Processing
-
Environment Setup:
- First and foremost, ensure that
openslide
is properly installed and configured on your system. This library is essential for handling WSIs. - Additionally, install
pma_python
to interact with Pathomation software for digital microscopy.
- First and foremost, ensure that
-
Converting WSIs:
- For WSIs saved in the
.mds
format, utilize theconvert2tiff.py
script to convert them to TIFF format. This conversion is crucial for compatibility with most image processing tools.
- For WSIs saved in the
-
Analyzing WSIs:
- To examine the layers within WSIs, run the
openslidetest1.py
script. This script allows you to print out and inspect the different layers of the images.
- To examine the layers within WSIs, run the
Ensure all dependencies and libraries are correctly installed before proceeding with the scripts above for a smooth and efficient workflow.
Reference
[1] https://www.pathomation.com/
[2] https://github.com/Pathomation/pma_python
[3] https://openslide.org/api/python/