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adapt paths relatively to ecDNA folder
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Madalina Giurgiu authored and Madalina Giurgiu committed Jan 16, 2023
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10 changes: 5 additions & 5 deletions genomics/circle-seq/README.md
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Expand Up @@ -17,13 +17,13 @@ mamba env create -f circleseq.yaml
Run the analysis for the PDX primary and relapse:
```
# setup variables
REF={zenodo}/circleseq/annotation/hs37d5_mm10.fa
REF=../genome/hs37d5_mm10.fa
PRIMARY_SAMPLE=PDXprimary
RELAPSE_SAMPLE=PDXrelapse
PRIMARY_1_FASTQ={zenodo}/circleseq/lane1PrimaryPDX_1_sequence.fq.gz
PRIMARY_2_FASTQ={zenodo}/circleseq/lane1PrimaryPDX_2_sequence.fq.gz
RELAPSE_1_FASTQ={zenodo}/circleseq/lane1RelapsePDX_1_sequence.fq.gz
RELAPSE_2_FASTQ={zenodo}/circleseq/lane1RelapsePDX_2_sequence.fq.gz
PRIMARY_1_FASTQ=ecDNA/lane1PrimaryPDX_1_sequence.fq.gz
PRIMARY_2_FASTQ=ecDNA/lane1PrimaryPDX_2_sequence.fq.gz
RELAPSE_1_FASTQ=ecDNA/lane1RelapsePDX_1_sequence.fq.gz
RELAPSE_2_FASTQ=ecDNA/lane1RelapsePDX_2_sequence.fq.gz
# run analysis
bash run.sh
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