Skip to content

Commit

Permalink
notes on cleaning
Browse files Browse the repository at this point in the history
  • Loading branch information
CamDavidsonPilon committed Nov 24, 2023
1 parent aaa30f9 commit 8a9f66e
Show file tree
Hide file tree
Showing 3 changed files with 16 additions and 11 deletions.
24 changes: 15 additions & 9 deletions user-guide/03-Extending your Pioreactor/07-using-pumps.md
Original file line number Diff line number Diff line change
Expand Up @@ -87,29 +87,35 @@ We strongly recommended you don't run peristaltic pumps *continuously* for hours

To avoid contamination, pumps should be sterilized before and after use.

#### Using heat

1. You can disassemble the pump head, extract the tubing, and autoclave the tubing. (If it's your first time, make note of how the pumps are put back together)
2. Put the tubing back in, reassemble the head, and your good to go.

#### Using bleach and ethanol

1. Create a 10% bleach dilution, or high concentration alcohol, in a container. Place the sink and source tubes of each pump you're sterilizing in the beaker.
2. On the UI, in the _Pioreactors_ tab, click _Manage_ and go to the _Dosing_ tab.
3. _Run continuously_ to cycle the disinfectant solution through each pump that you're using.
<img src="/img/user-guide/dosing_in_ui.png" width="735" style={{margin: "15px auto", display:"block"}}/>
4. _Interrupt_ after sufficient cycling, at least 2 minutes. Perform either of the following:
4. _Interrupt_ after sufficient cycling, at least 2 minutes.
5. If finishing an experiment, repeat the above with water. If starting an experiment, you'll need to prime the pumps, see the next section.

:::caution
It's important to not keep harsh chemicals, like bleach, in the silicone tubing for long. Cleaning pumps should always end with flushing the tubes with water or something inert. See below:
It's important to not keep harsh chemicals, like bleach, in the silicone tubing for long. Cleaning pumps should always end with flushing the tubes with water or something inert.
:::


#### A. Preparing for experiments
### Preparing for experiments

You can attach the source of your media pump to your media bottle and "prime" it by running the media pump until media comes out. The sink end can be left in a bleach/ethanol container, or attached to a Pioreactor vial.

5. From here, you can attach the source of your media pump to your media bottle and "prime" it by running the media pump until media, and not bleach solution, comes out. The sink end can be left in the bleach container, or attached to a Pioreactor vial.
6. Likewise, the waste pump can flushed with water, and then attached to the Pioreactor vial and waste bottle.
Likewise, the waste pump can flushed with water, and then attached to the Pioreactor vial and waste bottle.

:::tip
Before attaching to media, waste, or vials for your experiments, you can spray the luer lock ends with ethanol. Double check there's no liquid that may drip down.
Before attaching to media, waste, or vials for your experiments, you can spray the luer lock ends with ethanol. **Be careful that no liquid that drips down into the Pioreactor vial holder.**
:::

#### B. Cleaning
5. In another beaker with water, place the tubes in and repeat the cycling to rinse out the pumps.
6. Your pumps are now cleaned.


### Running pumps from the web interface
Expand Down
Original file line number Diff line number Diff line change
Expand Up @@ -53,7 +53,6 @@ There are three pumps on the Pioreactor: media, alt-media, and waste. You don't

:::info
You'll need the following:
1. A Pioreactor
2. A pump, with power connection to Pioreactor's PWM outputs
3. An accurate weighing scale
4. Container of water
Expand Down
2 changes: 1 addition & 1 deletion user-guide/99-common-questions.mdx
Original file line number Diff line number Diff line change
Expand Up @@ -70,7 +70,7 @@ Of course! The only two requirements are that the spectrum of the LED overlaps w

When you are using additional LEDs outside of the optical density LED & photodiode pair, you may worry that your additional LEDs will interfere with the optical density measurement. This won't happen, as we turn off all non-optical density LEDs before taking an optical density measurement.

### Is optical density the same as turbidity?
#### Is optical density the same as turbidity?

Turbidity is a measure of optical density, like how decibels are a measurement of sound intensity. Likewise, absorbance is a measure of optical density.

Expand Down

0 comments on commit 8a9f66e

Please sign in to comment.