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Benchmarking bioinformatic tools for amplicon based sequencing of norovirus

This repository contains the final version of scripts used to prepare data for simulation, simulation scripts, alongside the prepared pipelines and classifier scripts used to process the simulated sequencing data for the publication on benchmarking of bioinformatic tools for amplicon based sequencing of norovirus.

Pipelines compared include DADA2, Deblur, USEARCH-UNOISE3, VSEARCH and FROGS.

Classifiers compared include RDP, BLASTn, IDTAXA, QIIME2 naive Bayes and sintax.

Associated datasets and input files have been deposited at zenodo at https://zenodo.org/record/7271067#.Y2EeeXbP3IU

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