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Week04 Homework #114

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282 changes: 252 additions & 30 deletions weeks/week01/7_tf_binding_promoters.ipynb

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1 change: 1 addition & 0 deletions weeks/week01/README.md
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x
1 change: 1 addition & 0 deletions weeks/week01/biom262-hw1
Submodule biom262-hw1 added at 5b6660
1 change: 1 addition & 0 deletions weeks/week01/data/2
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Hello I am a message in standard error (stderr)
1,221 changes: 1,221 additions & 0 deletions weeks/week01/data/gencode.v19.annotation.chr22.transcript.promoter.nfkb.gtf

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18 changes: 18 additions & 0 deletions weeks/week03/.Rhistory
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lm <-read.table("littermates.txt",head=T)
lm
attach(lm)
names(lm)
mut
mean (mut)
medial (ctl)
median(ctl)
?median
?read
??read
plot(lm)
(q)
()q
[]q
q
quit
q()
1,288 changes: 1,288 additions & 0 deletions weeks/week03/R Practice in Class 1_19.ipynb

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59 changes: 46 additions & 13 deletions weeks/week04/0_alignment_expression_quantification.ipynb
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Expand Up @@ -270,7 +270,7 @@
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Expand All @@ -282,7 +282,18 @@
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"25"
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"5**2"
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Expand All @@ -317,14 +328,25 @@
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{
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"73"
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Expand All @@ -378,7 +411,7 @@
"0.000630957344480193"
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Expand All @@ -389,7 +422,7 @@
},
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Expand Down Expand Up @@ -505,7 +538,7 @@
}
},
"source": [
"YOUR ANSWER HERE"
"According to the gencode website, basic annotation \"identifies a subset of representative transcripts for each gene; prioritises full-length protein coding transcripts over partial or non-protein coding transcripts within the same gene, and intends to highlight those transcripts that will be useful to the majority of users.\" In contrats, the comprhensive annotation basically gives you all the information known about a gene including \"gene annotation on the reference chromosomes, scaffolds, assembly patches and alternate loci (haplotypes).\" The basic annotation is more straightforward but provides less information so I would want to use it if it provides information sufficient for the purpose of my study since it is more streamlined. "
]
},
{
Expand Down Expand Up @@ -857,7 +890,7 @@
}
},
"source": [
"1. -S\n",
"1. -S (though may not be necessary since it's the default)\n",
"2. -b\n",
"3. -@ 4"
]
Expand Down Expand Up @@ -968,7 +1001,7 @@
}
},
"source": [
"YOUR ANSWER HERE"
"If I were to use R or other programs I would use the tsv file. The h5 file would be used for kallisto's partner analysis software, sleuth."
]
},
{
Expand Down Expand Up @@ -1114,7 +1147,7 @@
}
},
"source": [
"YOUR ANSWER HERE"
"The featurecounts file was made by summing up all of the transcripts that matched to a given annotation where as the kallisto files gives an estimated abundance for each transcript through their \"pseudoalignment\" approach"
]
},
{
Expand Down
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