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real-sequences-note.md

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103 lines (92 loc) · 2.26 KB

All Iso-1

SRR22822929 ont-ligation SRR22822930 ont-ligation SRR23215007 ont-ligation SRR23215008 ont-ligation SRR23215010 ont-ligation SRR23215009 ont-ligation SRR11906526 pb-rs2 SRR11906525 pb-sequel iso1_hifi.fastq.gz pb-hifi iso1-R1041_ONT.fastq.gz ont-ligation iso1-R941-2_ONT.fastq.gz ont-ligation iso1-R941_ONT.fastq.gz ont-ligation A4_hifi_099.min6k.fasta.gz pb-hifi

SRR22822929

=SRX18782374 Q20 male adult flies LSK114 ligation kit MinION R10.4.1 flow cell at 400bps Guppy 6.4.2 in super-accuracy mode using the default quality filter.

SRR23215008

=SRX19162819 Q20 duplex only male adult flies LSK114 ligation kit MinION R10.4.1 flow cell at 400bps Duplex basecalling Guppy 6.4.2 in super-accuracy mode using the default quality filter duplex_tools

SRR23215007

=SRX19162820 Q20 simplex only LSK114 ligation kit MinION R10.4.1 flow cell at 400bps Duplex basecalling Guppy 6.4.2 in super-accuracy mode using the default quality filter duplex_tools Reads used for duplex calling were removed from the simplex read set

SRR22822930

=SRX18782373 Q20 male adult flies LSK114 ligation kit MinION R10.4.1 flow cell at 260bps Guppy 6.4.2 in super-accuracy mode using the default quality filter

SRR23215010

=SRX19162817 Q20 duplex only male adult flies LSK114 ligation kit MinION R10.4.1 flow cell at 260bps Duplex basecalling Guppy 6.4.2 in super-accuracy mode using the default quality filter duplex_tools

SRR23215009

=SRX19162818 Q20 simplex only male adult flies LSK114 ligation kit MinION R10.4.1 flow cell at 260bps Duplex basecalling Guppy 6.4.2 in super-accuracy mode using the default quality filter duplex_tools Reads used for duplex calling were removed from the simplex read set

SRR11906526

=SRX8453113 PacBio RS II female virgin 20 kb , BluePippin Size-Selection MagBead loading two SMART cells

SRR11906525

=SRX8453114 PACBIO_SMRT (Sequel) female virgin 20 kb , BluePippin Size-Selection MagBead loading two SMART cells

prefetch -pcv SRR11906526 SRR11906525

tech="ONT" for i in /pub/jenyuw/Eval-sv-temp/raw/{SRR22822929,SRR23215008,SRR23215007,SRR22822930,SRR23215010,SRR23215009}/*.sra do echo $i name=basename $i | sed "s/.sra/-R1041_${tech}.fastq.gz/" echo $name fastq-dump --split-spot --stdout $i | pigz -p 15 -v >/pub/jenyuw/Eval-sv-temp/raw/${name} done