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Update from the development fork #42

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b86dd0e
ensured all outputs are named by IDs and made output names more clear
zachary-foster Sep 13, 2023
4976227
added SNP alignment to test input data for main report
zachary-foster Sep 13, 2023
9f4654d
fixed a few channel operations breaking the cache
zachary-foster Sep 14, 2023
2e6c2a0
added depth-based read subsetting to speed up variant calling
zachary-foster Sep 14, 2023
ccb4557
changed report from bookdown to quarto
zachary-foster Sep 14, 2023
3d054d4
test
grunwald Sep 15, 2023
a6b736a
minor
grunwald Sep 15, 2023
c7559a0
random changes testing things
zachary-foster Sep 15, 2023
81357cd
merge
zachary-foster Sep 15, 2023
f0ba45d
remove python
grunwald Sep 15, 2023
110c016
added summary formatting
grunwald Sep 15, 2023
4022a0f
minor
grunwald Sep 15, 2023
de7408e
date format changed
grunwald Sep 15, 2023
ea780ea
date format
grunwald Sep 15, 2023
fb1790f
fixed table format
grunwald Sep 15, 2023
cbf17ec
minor
grunwald Sep 15, 2023
5db8232
changed to having all libaries loaded in a chunk at top for the rest …
grunwald Sep 15, 2023
39a1a86
minor edits
grunwald Sep 17, 2023
48c7a28
added draft about stuff. maybe this could be moved to one palced and …
grunwald Sep 17, 2023
d705f4b
added stats for # samples
grunwald Sep 18, 2023
d88aa68
submitter names parsed
grunwald Sep 18, 2023
7cd2d8e
minor edits
grunwald Sep 18, 2023
56163bb
minor edits
grunwald Sep 18, 2023
3275a98
minor edits
grunwald Sep 18, 2023
22e349f
minor changes
grunwald Sep 18, 2023
d666371
changes to about
grunwald Sep 19, 2023
bbedc80
added contributor
grunwald Sep 19, 2023
82e6ff0
added parameters to control subsampling
zachary-foster Sep 19, 2023
0671b04
Merge branch 'master' of github.com:grunwaldlab/pathogensurveillance
zachary-foster Sep 19, 2023
c4f54fe
various changes including methods section and first citations
grunwald Sep 19, 2023
dcb48fa
minor edit
grunwald Sep 19, 2023
1b0aca5
Just testing before making a few updates
Sep 20, 2023
e46be96
change to biblio ref
grunwald Sep 20, 2023
5a759fa
fix to subsampling code
zachary-foster Sep 20, 2023
1a1bd24
Merge branch 'dev' of github.com:grunwaldlab/pathogensurveillance int…
zachary-foster Sep 20, 2023
8369f1f
typo
grunwald Sep 20, 2023
79c191d
Merge branch 'dev' of github.com:grunwaldlab/pathogensurveillance int…
grunwald Sep 20, 2023
10ac12e
Adding comments and playing with MSN and SNP tree options
Sep 20, 2023
37f55f1
Updates to SNP tree
Sep 20, 2023
2871ab7
more test data inputs, but qmds still need updates
zachary-foster Sep 20, 2023
1980c74
Merge branch 'dev' of github.com:grunwaldlab/pathogensurveillance int…
zachary-foster Sep 20, 2023
b002ad7
ANI matrix explorations
Sep 20, 2023
195f723
updates-snp tree
Sep 20, 2023
f2fc479
Merge branch 'dev' of github.com:grunwaldlab/pathogensurveillance int…
Sep 20, 2023
827b644
quick updates to diversity report
Sep 20, 2023
ab52951
added parsing of new inputs
zachary-foster Sep 21, 2023
da80906
merge
zachary-foster Sep 21, 2023
e298860
updates to diversity.qmd
Sep 21, 2023
8acb412
Merge branch 'dev' of github.com:grunwaldlab/pathogensurveillance int…
Sep 21, 2023
43f7735
Quick updates to continue testing MSN functions
Sep 21, 2023
5f6abdc
Made report mostly work with new test data and pipeline
zachary-foster Sep 21, 2023
611a574
merge
zachary-foster Sep 21, 2023
a20da53
Early work on diversity.qmd-very rough!
Sep 21, 2023
9fc65d5
Quick updates to organization of diversity doc.
Sep 21, 2023
9ec744d
Quick fixes to headers as I figure out organization
Sep 21, 2023
cae766c
Updates to diversity.qmd
Sep 24, 2023
9b34995
minor
grunwald Sep 25, 2023
e3a02d6
tried to embed softre_Versions.yml file with all versions. should pro…
grunwald Sep 25, 2023
b1aa8c0
include statement reads software_versions.yml, but does not break lin…
grunwald Sep 25, 2023
60c6938
deleted embed
grunwald Sep 25, 2023
cfce1af
software versions used are now displayed.
grunwald Sep 25, 2023
e8ddc4d
Quick updates to render .qmd file
Sep 26, 2023
286dfb9
update
Sep 26, 2023
7b897f0
adding sketch table and still playing with it.
cahuparo Sep 28, 2023
61a2ffe
Sendsketch table draft done - Camilo
cahuparo Sep 28, 2023
0fbfbb2
make report output as a single HTML file
zachary-foster Sep 28, 2023
76a2a3b
added custom css
zachary-foster Sep 28, 2023
efc19f4
changed static table to interactive
cahuparo Sep 28, 2023
242adbd
removed top nav bar, added CSS
zachary-foster Sep 28, 2023
eb25f61
Merge branch 'dev' of github.com:grunwaldlab/pathogensurveillance int…
zachary-foster Sep 28, 2023
9585d2c
quick updates to diversity section
Sep 29, 2023
f24298d
minor theme changes
zachary-foster Sep 29, 2023
afa8b76
merge
zachary-foster Sep 29, 2023
c4904ff
fixed some errors when using non-test data
zachary-foster Sep 29, 2023
83d8cea
Added requirement for report group
marinawitherell Oct 2, 2023
41c40e0
Merge branch 'master' of github.com:grunwaldlab/pathogensurveillance
marinawitherell Oct 2, 2023
645827c
made input to main report just the run directory
zachary-foster Oct 2, 2023
85f9844
made new test data that is easier to switch between
zachary-foster Oct 2, 2023
48a0d8f
created a favicon, but it is not displaying where I want to.
grunwald Oct 3, 2023
8d0336a
updates
Oct 3, 2023
7b75920
Merge branch 'dev' of github.com:grunwaldlab/pathogensurveillance int…
Oct 3, 2023
c43e1a7
updates
Oct 3, 2023
884b638
Updates
Oct 3, 2023
8741604
make reports all go into the same output directory
zachary-foster Oct 3, 2023
17ad56c
fix software versions not being tracked for much of the pipeline
zachary-foster Oct 3, 2023
0642e43
Merge branch 'dev' of github.com:grunwaldlab/pathogensurveillance int…
zachary-foster Oct 3, 2023
f8dc9d5
fix bug due to no taxa being found in sendsketch results
zachary-foster Oct 3, 2023
9828665
adding while loop to improve reference picking
cahuparo Oct 3, 2023
6a87d1d
SNP tree revisions
Oct 4, 2023
b7c1aa1
SNP tree update
Oct 4, 2023
73dcb12
Revising how sample names parsed for SNP analyses
Oct 4, 2023
95f1411
fixed divide by zero error when attempting to subsample low-depth sam…
zachary-foster Oct 4, 2023
ff32db7
Merge branch 'dev' of github.com:grunwaldlab/pathogensurveillance int…
zachary-foster Oct 4, 2023
7247e2a
Merge branch 'master' into dev
zachary-foster Oct 4, 2023
bb67b29
revise tip labels and strain names-Snp analysis
Oct 4, 2023
7e51955
Merge branch 'dev' of github.com:grunwaldlab/pathogensurveillance int…
Oct 4, 2023
359fd63
SNP analysis update
Oct 4, 2023
b5dec44
added a footer with osu and usda logos.
grunwald Oct 4, 2023
76470fa
added fotoer and logos
grunwald Oct 4, 2023
9b766d8
minor edits
grunwald Oct 4, 2023
4f08ab3
Merge branch 'dev' of github.com:grunwaldlab/pathogensurveillance int…
grunwald Oct 4, 2023
2ab9c50
Minor edits to headers
grunwald Oct 4, 2023
b15e95c
moved about seciton to end
grunwald Oct 4, 2023
3c66e91
added nf-core logo
grunwald Oct 4, 2023
df7f0d8
minor edits
grunwald Oct 4, 2023
71d74e0
more detials added to footer
grunwald Oct 4, 2023
88fe796
typo
grunwald Oct 4, 2023
f67c113
minor edit
grunwald Oct 4, 2023
0228e17
ANI tree
Oct 4, 2023
9c6013b
Merge branch 'dev' of github.com:grunwaldlab/pathogensurveillance int…
Oct 4, 2023
620ab97
started work on handling errors/warnings and passing them to the report
zachary-foster Oct 5, 2023
5992793
Merge branch 'dev' of github.com:grunwaldlab/pathogensurveillance int…
zachary-foster Oct 5, 2023
b932539
ggtree plotly updates
Oct 5, 2023
1943057
Merge branch 'dev' of github.com:grunwaldlab/pathogensurveillance int…
Oct 5, 2023
db9a647
added icon collection and some icon images
grunwald Oct 5, 2023
af12f40
added icons
grunwald Oct 5, 2023
a14bb14
more icons
grunwald Oct 5, 2023
9ac75b7
added acknowledgement for icon libraries.
grunwald Oct 5, 2023
1fb61b9
change to feedback item
grunwald Oct 5, 2023
d6a2967
icon added
grunwald Oct 5, 2023
3fde3be
added R logo
grunwald Oct 5, 2023
5d66729
minor edit
grunwald Oct 5, 2023
8adbaf1
added nf-core reference
grunwald Oct 5, 2023
44c66eb
undo testing change
zachary-foster Oct 6, 2023
247e3d3
Merge branch 'dev' of github.com:grunwaldlab/pathogensurveillance int…
zachary-foster Oct 6, 2023
3065cf7
more work on trying to get status updates to the main report
zachary-foster Oct 6, 2023
6f78adb
updates
Oct 10, 2023
9678de3
Merge branch 'dev' of github.com:grunwaldlab/pathogensurveillance int…
Oct 10, 2023
591be64
minor
zachary-foster Oct 10, 2023
5c6fd06
Merge branch 'dev' of github.com:grunwaldlab/pathogensurveillance int…
zachary-foster Oct 10, 2023
3365a6a
fixes versiondump bug
zachary-foster Oct 10, 2023
6fe9bb3
redid main report inputs to allow for missing inputs from skipped pro…
zachary-foster Oct 10, 2023
fc8dbc8
treechart to report % of samples clasified as X taxa - needs more work.
cahuparo Oct 11, 2023
41e7a36
comments from Nik
grunwald Oct 11, 2023
90145fd
disable circos plotting in bakta that sometimes causes errors
zachary-foster Oct 12, 2023
780d52a
removed unneeded nesting in main workflow to make progress printing b…
zachary-foster Oct 13, 2023
05a2895
Stop optional columns with commas causing incorrect CSV parsing
zachary-foster Oct 13, 2023
9689a21
display the whole taxonomy with plotly using the `sunburst` and `tree…
zachary-foster Oct 13, 2023
b7254f9
update
Oct 14, 2023
7598181
Merge branch 'dev' of github.com:grunwaldlab/pathogensurveillance int…
Oct 14, 2023
99ca083
Updates to NJ tree
Oct 16, 2023
84b0d7b
images
grunwald Oct 16, 2023
ba5cf2d
icon libraries
grunwald Oct 16, 2023
a434605
Merge branch 'dev' of github.com:grunwaldlab/pathogensurveillance int…
grunwald Oct 16, 2023
d57040a
added 'color_by' column to example data
zachary-foster Oct 17, 2023
469a179
Merge branch 'dev' of github.com:grunwaldlab/pathogensurveillance int…
zachary-foster Oct 17, 2023
a2b0244
Adding a few notes
Oct 17, 2023
5e39045
Ensure that reference IDs are assigned to a single unique reference …
zachary-foster Oct 17, 2023
5b4e819
Merge branch 'dev' of github.com:grunwaldlab/pathogensurveillance int…
zachary-foster Oct 17, 2023
ec55a59
Notes for diversity section
Oct 17, 2023
cef412c
Updates to ANI outputs
Oct 20, 2023
de5a01d
test graphics from dash-plotly-python and observable JS
grunwald Oct 20, 2023
fb985b8
Merge branch 'dev' of github.com:grunwaldlab/pathogensurveillance int…
grunwald Oct 20, 2023
1f5f4e0
tree updates
Oct 20, 2023
0876deb
Merge branch 'dev' of github.com:grunwaldlab/pathogensurveillance int…
Oct 20, 2023
e4976bf
small edits
grunwald Oct 20, 2023
ffd8981
new
zachary-foster Oct 20, 2023
7f9d6a3
Merge branch 'dev' of github.com:grunwaldlab/pathogensurveillance int…
zachary-foster Oct 20, 2023
5f2bac6
example plotly ggrtree with reference to url
grunwald Oct 20, 2023
d0680e9
Updates to ANI tree
Oct 23, 2023
0e79b16
Merge branch 'dev' of github.com:grunwaldlab/pathogensurveillance int…
Oct 23, 2023
72cabcc
tree output updates
Oct 23, 2023
6b3885d
Tree updates
Oct 23, 2023
2dcf9b4
Make more informative labels for tree and ANI matrix
Oct 23, 2023
d204984
MSN updates
Oct 24, 2023
e4bf806
Updates poppr portion
Oct 25, 2023
a6eb343
Testing different MSN outputs
Oct 25, 2023
8759df8
Quick changes MSN
Oct 31, 2023
2081900
trying to fix duplicated logo on readme
zachary-foster Nov 2, 2023
1c54615
clean up report and move images to their own directory
zachary-foster Nov 2, 2023
155a7d7
merge
zachary-foster Nov 2, 2023
ab41192
Bib update
Nov 9, 2023
69eb782
Updates
Nov 9, 2023
b2a8f5c
added busco module
zachary-foster Nov 9, 2023
e58f667
ggtree update to render doc more cleanly
Nov 14, 2023
59dc3f8
Tasks added after meeting today
Nov 14, 2023
39bb0b9
midwork on busco and core gene phylo filtering
zachary-foster Nov 17, 2023
0019cbc
Merge branch 'dev' of github.com:grunwaldlab/pathogensurveillance int…
zachary-foster Nov 17, 2023
2543f7c
Quick updates to MSN portion of report.
Nov 27, 2023
3231925
Updates to MSN portion of report
Nov 27, 2023
9d7043d
minor edits. added Ricardo
grunwald Nov 29, 2023
43e5b80
MSN upate
Dec 6, 2023
421b87d
Merge branch 'dev' of github.com:grunwaldlab/pathogensurveillance int…
Dec 6, 2023
fedb415
working on changes to how ID are handled; midwork
zachary-foster Dec 14, 2023
e80bd72
Merge branch 'dev' of github.com:grunwaldlab/pathogensurveillance int…
zachary-foster Dec 14, 2023
ea9b092
make test data work on tower
zachary-foster Dec 14, 2023
80bf308
change input paths to URLs
zachary-foster Dec 14, 2023
d69caef
fix typo
zachary-foster Dec 14, 2023
d7a276d
update docker container
zachary-foster Dec 14, 2023
9a57fcf
new abstracts
zachary-foster Dec 15, 2023
d0d2efd
updating modules to fix docker issues
zachary-foster Dec 15, 2023
4b4d031
debugging google cloud
zachary-foster Dec 15, 2023
48998c7
changed how R scripts are called
zachary-foster Dec 16, 2023
d4e7d6e
typo fix
zachary-foster Dec 16, 2023
0f0fbbb
Merge branch 'master' into dev
zachary-foster Dec 22, 2023
55b755d
fix
zachary-foster Dec 22, 2023
56bd22b
fixing merge problems
zachary-foster Dec 23, 2023
016d4cd
testing fix for reference download error on tower
zachary-foster Dec 23, 2023
6bb8825
trying to fix tower bug
zachary-foster Dec 23, 2023
becf499
trying out a custom docker image
zachary-foster Dec 23, 2023
5a0aa31
reduce cpu usage to meet google cloud quotas
zachary-foster Dec 24, 2023
c3e67bb
increase max concurrent downloads
zachary-foster Dec 24, 2023
27a8bcc
made location of bakta database variable
zachary-foster Jan 2, 2024
ad08799
fix ubuntu docker paths
zachary-foster Jan 3, 2024
fb2f855
add docker files
zachary-foster Jan 3, 2024
788541f
testing quay.io automated docker builds
zachary-foster Jan 3, 2024
cdb92e8
more testing of docker builds
zachary-foster Jan 3, 2024
17eda67
bug fixes
zachary-foster Jan 3, 2024
af3855b
trying to make pirate run faster for the small test dataset
zachary-foster Jan 3, 2024
9862bd4
remove API key
zachary-foster Jan 3, 2024
becb365
prepare for main report rendering in docker
zachary-foster Jan 4, 2024
4798cc5
increase memory for khmer_trimlowabund
zachary-foster Jan 4, 2024
cdcb30d
hard code path to bakta database on gogole storage
zachary-foster Jan 4, 2024
b6aa0e0
add code to download the bakta database
zachary-foster Jan 4, 2024
b56ce22
lower used resources for testing on google cloud
zachary-foster Jan 4, 2024
ba27333
testing out docker file for main report
zachary-foster Jan 5, 2024
6ed03fd
made docker image work for report template
zachary-foster Jan 8, 2024
549e6a8
increase max resources used for test dataset
zachary-foster Jan 8, 2024
24ba89f
add main report template as channel
zachary-foster Jan 9, 2024
88d32d0
trying to get reports to be downloadable on tower
zachary-foster Jan 9, 2024
1108d38
remove outdir path from test config
zachary-foster Jan 9, 2024
d8aad7d
enabled busco subworkflow
zachary-foster Jan 11, 2024
f46a2c7
updated documentation
zachary-foster Jan 18, 2024
89c6b30
restored old report for demo
zachary-foster Jan 19, 2024
f51ba91
testing out psminer
zachary-foster Jan 19, 2024
6d71690
update docker image version used for report
zachary-foster Jan 19, 2024
43a7959
temporarily disable busco
zachary-foster Jan 19, 2024
2786e17
fix report error on tower
zachary-foster Jan 19, 2024
a6f3c68
add ggdendro to dockerfile
zachary-foster Jan 19, 2024
0ff5a33
added DT pacakge to dockerfile
zachary-foster Jan 22, 2024
a750286
Updates to report-psminer troubleshooting
Jan 22, 2024
201430c
Simplify ANI command
Jan 23, 2024
70e3648
Updates incorporating psminer fxns
Jan 23, 2024
d0710fb
Update report as we test out psminer fxns
Jan 23, 2024
1036f68
fixed bug extracting taxononmy when no genus was present in classific…
zachary-foster Jan 23, 2024
d93ff95
Merge branch 'master' of github.com:grunwaldlab/pathogensurveillance
zachary-foster Jan 23, 2024
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31 changes: 24 additions & 7 deletions README.md
Original file line number Diff line number Diff line change
@@ -1,4 +1,9 @@
# ![nf-core/pathogensurveillance](docs/images/nf-core-pathogensurveillance_logo_light.png#gh-light-mode-only) ![nf-core/pathogensurveillance](docs/images/nf-core-pathogensurveillance_logo_dark.png#gh-dark-mode-only)
<picture>
<source media="(prefers-color-scheme: dark)" srcset="docs/images/nf-core-pathogensurveillance_logo_dark.png">
<source media="(prefers-color-scheme: light)" srcset="docs/images/nf-core-pathogensurveillance_logo_light.png">
<img alt="nf-core/pathogensurveillance" src="docs/images/nf-core-pathogensurveillance_logo_light.png">
</picture>


[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/pathogensurveillance/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX)

Expand All @@ -17,14 +22,18 @@
<!-- TODO nf-core: Write a 1-2 sentence summary of what data the pipeline is for and what it does -->

**nf-core/pathogensurveillance** is a population genomic pipeline for pathogen diagnosis, variant detection, and biosurveillance.
The pipeline accepts the paths to raw reads for one or more organisms and creates reports in the form of interactive HTML reports or PDF documents.
Significant features include the ability to analyze unidentified eukaryotic and prokaryotic samples, creation of reports for multiple user-defined groupings of samples, automated discovery and downloading of reference assemblies from NCBI RefSeq, and rapid initial identification based on k-mer sketches followed by a more robust core genome phylogeny.
The pipeline accepts the paths to raw reads for one or more organisms (in the form of a CSV file) and creates reports in the form of interactive HTML reports or PDF documents.
Significant features include the ability to analyze unidentified eukaryotic and prokaryotic samples, creation of reports for multiple user-defined groupings of samples, automated discovery and downloading of reference assemblies from NCBI RefSeq, and rapid initial identification based on k-mer sketches followed by a more robust core genome phylogeny and SNP-based phylogeny.

The pipeline is built using [Nextflow](https://www.nextflow.io), a workflow tool to run tasks across multiple compute infrastructures in a very portable manner. It uses Docker/Singularity containers making installation trivial and results highly reproducible. The [Nextflow DSL2](https://www.nextflow.io/docs/latest/dsl2.html) implementation of this pipeline uses one container per process which makes it much easier to maintain and update software dependencies. Where possible, these processes have been submitted to and installed from [nf-core/modules](https://github.com/nf-core/modules) in order to make them available to all nf-core pipelines, and to everyone within the Nextflow community!
The pipeline is built using [Nextflow](https://www.nextflow.io), a workflow tool to run tasks across multiple compute infrastructures in a very portable manner.
It uses Docker/Singularity containers making installation trivial and results highly reproducible.
The [Nextflow DSL2](https://www.nextflow.io/docs/latest/dsl2.html) implementation of this pipeline uses one container per process which makes it much easier to maintain and update software dependencies.
Where possible, these processes have been submitted to and installed from [nf-core/modules](https://github.com/nf-core/modules) in order to make them available to all nf-core pipelines, and to everyone within the Nextflow community!

<!-- TODO nf-core: Add full-sized test dataset and amend the paragraph below if applicable -->

On release, automated continuous integration tests run the pipeline on a full-sized dataset on the AWS cloud infrastructure. This ensures that the pipeline runs on AWS, has sensible resource allocation defaults set to run on real-world data sets, and permits the persistent storage of results to benchmark between pipeline releases and other analysis sources.The results obtained from the full-sized test can be viewed on the [nf-core website](https://nf-co.re/pathogensurveillance/results).
On release, automated continuous integration tests run the pipeline on a full-sized dataset on the AWS cloud infrastructure.
This ensures that the pipeline runs on AWS, has sensible resource allocation defaults set to run on real-world data sets, and permits the persistent storage of results to benchmark between pipeline releases and other analysis sources.The results obtained from the full-sized test can be viewed on the [nf-core website](https://nf-co.re/pathogensurveillance/results).

## Pipeline summary

Expand All @@ -39,7 +48,7 @@ On release, automated continuous integration tests run the pipeline on a full-si
3. Download the pipeline and test it on a minimal dataset with a single command:

```bash
nextflow run nf-core/pathogensurveillance -profile test,YOURPROFILE --outdir <OUTDIR>
nextflow run nf-core/pathogensurveillance -profile test,YOURPROFILE --outdir <OUTDIR> -resume
```

Note that some form of configuration will be needed so that Nextflow knows how to fetch the required software. This is usually done in the form of a config profile (`YOURPROFILE` in the example command above). You can chain multiple config profiles in a comma-separated string.
Expand All @@ -54,9 +63,17 @@ On release, automated continuous integration tests run the pipeline on a full-si
<!-- TODO nf-core: Update the example "typical command" below used to run the pipeline -->

```bash
nextflow run nf-core/pathogensurveillance --input samplesheet.csv --outdir <OUTDIR> --genome GRCh37 -profile <docker/singularity/podman/shifter/charliecloud/conda/institute>
nextflow run nf-core/pathogensurveillance --input samplesheet.csv --outdir <OUTDIR> -profile <docker/singularity/podman/shifter/charliecloud/conda/institute> -resume
```

You can also try running a small example dataset hosted with the source code using the following command (no need to download anything):

```
nextflow run nf-core/pathogensurveillance --input https://raw.githubusercontent.com/grunwaldlab/pathogensurveillance/master/test/data/metadata_small.csv --outdir test_out --download_bakta_db true -profile docker -resume
```



## Documentation

The nf-core/pathogensurveillance pipeline comes with documentation about the pipeline [usage](https://nf-co.re/pathogensurveillance/usage), [parameters](https://nf-co.re/pathogensurveillance/parameters) and [output](https://nf-co.re/pathogensurveillance/output).
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