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## Table of Contents {#table-of-contents}
# Introduction to the step-by-step guide {#introduction-to-the-step-by-step-guide}

[Table of Contents](#table-of-contents)
Here are step-by-step instructions on how to run the FlowJo stat workflow.
This workflow allows the automation and parallelization of many plot analyses.

[Introduction to the step by step guide](#introduction-to-the-step-by-step-guide)

[Create Project](#create-project)

[Preparation of the 3 files](#preparation-of-the-3-files)

[Uploading the data](#uploading-the-data)

[Finding the template](#finding-the-template)

[Open the workflow](#open-the-workflow)

[Gather Step](#gather-step)

[Join Step](#join-step)

[Wizard Step](#wizard-step)

[Checking if all the annotation are correct](#checking-if-all-the-annotation-are-correct)

[Create Filter](#create-filter)

[Customization of Plots](#customization-of-plots)

[Download the Data and Plots](#download-the-data-and-plots)

## Introduction to the step by step guide {#introduction-to-the-step-by-step-guide}

Here is a step by step instruction on how to run the FlowJo stat workflow.
It is a workflow allowing automation and parallelization of many plots analysis.

## Create Project {#create-project}
# Create Project

Create a Project in Tercen
Note: If you create in a Team then you can share it easier
Note: If you create in a Team, then you can share it easier

## Preparation of the 3 files {#preparation-of-the-3-files}
# Preparation of the three files

You require three files

Expand All @@ -49,31 +19,31 @@ You require three files
These can be created from the Flowjo Export Table file

Here is a link to a template of the three files.
Download them and fill them.
Download them and fill them out.

## Uploading the data {#uploading-the-data}
# Uploading the data {#uploading-the-data}

When uploading the data:

* ATP import as “character”
* SEB import as “character”
* Days import as “character”

## Finding the template {#finding-the-template}
# Finding the template

I copied it from a project
I copied it from a project.

## Open the workflow {#open-the-workflow}
# Open the workflow

### Gather Step {#gather-step}
## Gather Step

* Remove all and add all

### Join Step {#join-step}
## Join Step

Run the join

### Wizard Step {#wizard-step}
## Wizard Step

| Question | Action | Remark |
| ----- | :---: | ----- |
Expand All @@ -89,19 +59,19 @@ Run the join
| "Select the factors representing the conditions. Multiple selections are possible. Examples: \`sample.compound\`, \`sample.ATP\`, \`sample.Ab\`, \`sample.Ab2\` and etc..", | sample.Ab sample.Ab2 sample.treatment sample.STP sample.SEB | Manually selected |
| Selecting the control per condition (z-score on mean) Filters \- Select your control compound/condition | sample.treatment \= DMSO ATP \=0 SEB \=1 Ab= PD1 Ab2 \= Belrestotug | Manually selected Apply filter |

### Checking if all the annotation are correct {#checking-if-all-the-annotation-are-correct}
## Checking if all the annotations are correct

Checking if all there is one single value per cell
Checking if there is one single value per cell

### Create Filter {#create-filter}
## Create Filter

## Customization of Plots {#customization-of-plots}
# Customization of Plots

| Point plot | 2Gb |
| :---- | :---- |
| Barplot (mean) with jitter | 2Gb |
| Barplot (mean) with sd error bars | 2Gb |
| Heatmap | 2Gb, 1000x3000 |

## Download the Data and Plots {#download-the-data-and-plots}
# Download the Data and Plots

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