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JavaScript and most of the other programming languages uses 0 based indexing and half open intervals. i.,e interval(start, end) is exclusive of the end
The Feature data and gff files use 1 based indexing.
A padding of a single is added before every refSeq sequence so that the 1 based indexing of genomic Features can be used with 0 based indexing of JavaScript without any explicit conversion.
start and end intervals of the genomic Feature data are also half open intervals. This mean the base pair at the end coordinate is not a part of the given feature.