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Update README.md
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Millreefer Gao authored Apr 23, 2021
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Expand Up @@ -18,7 +18,7 @@ docker pull mingxuangao/dreamdia:v1.0.0
docker run -it --name dreamdia_example --gpus all -v /YOUR/OWN/WORK/PATH:/tmp/work mingxuangao/dreamdia:v1.0.0 /bin/bash
```

* Using the argument `--gpus all` will considerably accelerate the Dream-DIA-XMBD pipeline if you have some GPUs.
* Using the argument `--gpus all` will considerably accelerate the DreamDIA-XMBD pipeline if you have some GPUs.

3. Activate the conda environment.

Expand Down Expand Up @@ -58,8 +58,8 @@ If .raw files are going to be fed directly to Dream-DIA-XMBD in Linux systems, [
### Installation

```bash
git clone https://github.com/xmuyulab/Dream-DIA-XMBD
cd Dream-DIA-XMBD
git clone https://github.com/xmuyulab/DreamDIA-XMBD
cd DreamDIA-XMBD
bash build.sh
```

Expand All @@ -79,7 +79,7 @@ python Dream-DIA/DreamDIA.py dreamscore --file_dir rawdata_dir --lib library.tsv

* Centroided .mzML or .mzXML files are supported at any time.

* If .raw files are going to be fed directly to Dream-DIA-XMBD in Linux systems, [mono](https://www.mono-project.com/download/stable/#download-lin) must be installed first for the data format conversion by ThermoRawFileParser.
* If .raw files are going to be fed directly to DreamDIA-XMBD in Linux systems, [mono](https://www.mono-project.com/download/stable/#download-lin) must be installed first for the data format conversion by ThermoRawFileParser.

* You can also use our docker image to process .raw files directly, while it may take some time for data format conversion.

Expand All @@ -99,11 +99,11 @@ All raw data files should be at the same directory as below.

#### 2. Spectral libraries

Only .tsv libraries are supported. All of the fields required by Dream-DIA-XMBD are listed in `Dream-DIA/lib_col_settings`. Users can modify this file to adjust their own spectral libraries.
Only .tsv libraries are supported. All of the fields required by DreamDIA-XMBD are listed in `Dream-DIA/lib_col_settings`. Users can modify this file to adjust their own spectral libraries.

#### 3. window setting file

Dream-DIA-XMBD needs a tab separated window setting file **without overlapping** among the isolation windows as OpenSWATH. An example window setting file for classical SWATH acquisition strategy is shown below.
DreamDIA-XMBD needs a tab separated window setting file **without overlapping** among the isolation windows as OpenSWATH. An example window setting file for classical SWATH acquisition strategy is shown below.

```
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Expand Down Expand Up @@ -142,4 +142,4 @@ Dream-DIA-XMBD needs a tab separated window setting file **without overlapping**

#### 4. output

Dream-DIA-XMBD outputs peptide and protein identification and quantification results. An empty directory is suggested for the `--out` argument to save all of the output files.
DreamDIA-XMBD outputs peptide and protein identification and quantification results. An empty directory is suggested for the `--out` argument to save all of the output files.

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